Optimization of Ultrahigh Pressure Extraction of Momordicosides from Bitter Melon

2010 ◽  
Vol 6 (6) ◽  
Author(s):  
Hongfang Ji ◽  
Lingwen Zhang ◽  
Jian Li ◽  
Mingduo Yang ◽  
Xin Liu
Keyword(s):  
Ethanol Concentration ◽  
Maximum Yield ◽  
Dry Weight ◽  
Extraction Time ◽  
Ultrahigh Pressure ◽  
Bitter Melon ◽  
Hplc Profile ◽  
Pressure Extraction ◽  
Independent Parameters ◽  
Sample Ratio

Response surface methodology was used to optimize the conditions of ultrahigh pressure extraction of momordicosides from fresh fruits of bitter melon. The effect of extraction pressure, extraction time, ethanol concentration and solvent to sample ratio, and their interactions on yield of total momordicosides was investigated. Results showed that data could be well fitted to a second-order polynomial model. The independent parameters of extraction pressure, extraction time, solvent to sample ratio, all quadratics as well as the interactions between extraction pressure and solvent to sample ratio affected the yield significantly (p < 0.05). The model predicted that the optimal conditions were 423.1 MPa, extraction time 7.0 min, ethanol concentration 70.0 percent (V/V) and solvent to sample ratio 45.3 : 1 mL/g, and under such conditions, the maximum yield of 3.270 g Rg1 equivalents /100 g bitter melon dry weight could be achieved. In comparison with conventional heat reflux extraction, ultrahigh pressure extraction is more efficient and rapid to extract momordicosides from bitter melon. Additionally, the momordicosides compositions in extracts by both the methods were similar, which were investigated by using HPLC profile.

Effects of Ultrahigh Pressure Extraction Conditions on Yields and Antioxidant Activities of Flavonoid from Laoying Tea

2011 ◽  
Vol 7 (5) ◽  
Author(s):  
Hongfang Ji ◽  
Lingwen Zhang ◽  
Ailin Du ◽  
Mingduo Yang ◽  
Qing Wang
Keyword(s):  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Maximum Yield ◽  
Radical Scavenging ◽  
Holding Time ◽  
Water Bath ◽  
Model Systems ◽  
Ultrahigh Pressure ◽  
Pressure Extraction ◽  
Sample Ratio

Ultrahigh pressure extraction technique was employed to extract flavonoids from Laoying tea. Orthogonal design was applied to evaluate the effects of four independent factors (extraction pressure, pressure holding time, ethanol concentration, and solvent to sample ratio) on the yields of total flavonoids. Subsequently, the antioxidant activities of ultrahigh pressure extracts and conventional water bath extracts were analyzed by employing two antioxidant model systems including 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power. Results showed that the optimal conditions of UHPE were obtained at extraction pressure 400 MPa, pressure holding time 10 min, ethanol concentration 70% (V/V), and solvent to sample ratio 50:1 mL/g, and under such conditions, the maximum yield of 6.237 g quercetin equivalents /100 g Laoying tea dry weight could be achieved. Three flavonoid compounds, namely quercetin-3-O-β-D-galactoside, quercetin-3-O-β-D-glucoside, and kaempferol-3-O-β-D-glucoside were identified and quantified by HPLC. Compared with conventional water bath extraction, ultrahigh pressure extraction exhibited higher extraction effectiveness in terms of higher extraction yield, higher antioxidant activity with lower temperature and shorter extraction time.

scholarly journals Deep Eutectic Solvent-Based Ultrahigh Pressure Extraction of Baicalin from Scutellaria baicalensis Georgi

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3233 ◽  
Author(s):  
Hui Wang ◽  
Xiaodi Ma ◽  
Qibin Cheng ◽  
Li Wang ◽  
Liwei Zhang
Keyword(s):  
Extraction Method ◽  
Choline Chloride ◽  
Maximum Yield ◽  
Extraction Yield ◽  
Scutellaria Baicalensis ◽  
Extraction Time ◽  
Ultrahigh Pressure ◽  
Scutellaria Baicalensis Georgi ◽  
Solid Ratio ◽  
Pressure Extraction

Deep eutectic solvents (DESs), promising green solvents, and ultrahigh pressure extraction (UPE) as an effective auxiliary extraction method, have attracted wide attention. In this study, DES was coupled with UPE to efficiently extract baicalin from Scutellaria baicalensis Georgi. First, choline chloride: lactic acid (ChCl-LA, molar ratio 1:1) was selected as the most appropriate DES by comparing the extraction yield of different DESs. Second, the extraction protocol was optimized by response surface methodology (RSM) considering the impacts of ChCl-LA concentration, extraction pressure, extraction time and liquid-solid ratio on the extraction yield. Under the optimal condition (40 vol% water content, extraction pressure of 400 MPa, extraction time of 4 min and a liquid-solid ratio of 110 mL/g), a maximum yield of 116.8 mg/g was achieved, higher than that obtained by the traditional extraction method. The microstructure of the raw and extracted Scutellaria baicalensis Georgi samples according to scanning electron microscope (SEM) images revealed that the dissolution of chemical components was enhanced from the disrupted root tissues after DESs-UPE. DESs coupled with UPE could effectively extract the baicalin from Scutellaria baicalensis Georgi as a rapid and efficient extraction method.

scholarly journals Extraction Process of Polyphenols from Soybean (Glycine max L.) Sprouts: Optimization and Evaluation of Antioxidant Activity

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 489 ◽  
Author(s):  
Xuan Tien Le ◽  
Vo Luu Lan Vi ◽  
Tran Quoc Toan ◽  
Long Giang Bach ◽  
Tran Thanh Truc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Dry Weight ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Polyphenol Content ◽  
Experimental Conditions ◽  
Total Polyphenol ◽  
Total Polyphenol Content

This research aimed to optimize the total polyphenol content (TPC) extracted from soybean sprout powder under different experimental parameters, including ethanol concentration (60–100% v/v), extraction temperature (40–80 °C), extraction time (15–150 min), material:solvent ratio (1:4–1:10 g/mL), the number extraction cycles (1, 2 and 3 times), the age of sprout (0–7 days), and the used part of the sprout (cotyledon, hypocotyl, or radicle). The obtained results were used in response surface methodology, in combination with a central composite design, to model the total polyphenol content (TPC) with respect to three variables, including ethanol concentration, extraction temperature, and material:solvent ratio. The experimental conditions for optimal recovery of TPC consisted of ethanol concentration of 88% (v/v), extraction temperature of 59 °C, material:solvent ratio of 1:6.5 g/mL, extraction time of 60 min, and 2 cycles of maceration. In addition, for maximal TPC, the sprout should undergo the germination of 5 days and the radicle fraction should be used. Based on the suggested optimum conditions, the obtained and verified TPC was 19.801 mg genistein (GE)/g dry weight (d.w.). The obtained dried extract also exhibited low antioxidant activity.

scholarly journals Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 678
Author(s):  
Ao Shang ◽  
Min Luo ◽  
Ren-You Gan ◽  
Xiao-Yu Xu ◽  
Yu Xia ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Sample Ratio

In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows—ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 ℃ and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 ± 4.42 μM Fe(II)/g dry weight (DW), 613.11 ± 9.32 μM Trolox/g DW and 135.94 ± 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases.

Extraction of Fish Oil from the Muscle of Sturgeon Using Supercritical Fluids

2013 ◽  
Vol 655-657 ◽  
pp. 1975-1981 ◽  
Author(s):  
Shu Xian Hao ◽  
Hui Huang ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Jian Wei Cen ◽  
...  
Keyword(s):  
Fish Oil ◽  
Supercritical Fluids ◽  
Flow Rate ◽  
Polynomial Regression ◽  
Maximum Yield ◽  
Extraction Time ◽  
Coefficient Of Determination ◽  
Acipenser Baeri ◽  
Pressure Extraction ◽  
Co2 Flow

This study evaluated the extraction of oil from sturgeon (Acipenser baeri) muscle using supercritical fluids. Response surface methodology (RSM) was used to identify and quantify the variables, namely extraction pressure, extraction time and CO2 flow rate on the yield of oil. Statistical analysis indicated that for all three variables, the quadratic terms and interactions between the variables had significant effects on yield (p < 0.05). Polynomial regression model predictions were in good agreement with the experimental results, with a coefficient of determination of 0.9936 for yield. Maximum yield from sturgeon muscle was 26.83% with a pressure of 315.8 bar, extraction time of 10.8 min and CO2 flow rate of 3.5 l/min, which closely matched the predicted value (26.70%). The characteristics of the fish oil extracted with the supercritical fluids were superior to those of oil obtained by other methods.

scholarly journals EKSTRAKSI MULTI TAHAP KURKUMIN DARI TEMULAWAK (Curcuma xanthorriza Roxb.) MENGGUNAKAN PELARUT ETANOL

2015 ◽  
Vol 4 (2) ◽  
pp. 39-45 ◽  
Author(s):  
Dwimas Anggoro ◽  
Rajian Sobri Rezki ◽  
Siswarni MZ
Keyword(s):  
Extraction Method ◽  
Ethanol Concentration ◽  
Maximum Yield ◽  
Yellow Crystal ◽  
Extraction Time ◽  
Solvent Concentration ◽  
Multi Stage ◽  
Three Stages ◽  
Two Stages ◽  
Efficiency Rate

Curcumin is a pigment in turmeric (Curcuma xanthorriza Roxb.), which has an orange-yellow crystal appearance, and commonly being used as a colouring agent. Extraction method which is used in extracting curcumin from turmeric is one stage extraction. This research will study a multi stage extraction of curcumin from turmeric. The purposes of this research is to find the best condition in extracting curcumin using multi stages extraction method to increase the efficiency rate in curcumin extraction. This research used ethanol as a solvent, and effect from variables such as extraction time, solvent concentration, and number of extraction stages are observed. Extraction times are 60 minutes, 120 minutes, and 180 minutes. Variations of ethanol concentration are 50%, 70%, and 96%. Stage numbers of extraction are two stages extraction and three stages extraction. Extracts of curcumin are examined with qualitative analysis and quantitative analysis. The best condition of multi stages curcumin extraction is determined based on yield and content of curcumin. The maximum yield obtained in this study was 16,35 % with conditions 180 minutes extraction time, 96% ethanol concentration, and three stages extraction. The highest content of curcumin obtained is 2,617% with conditions 180 minutes extraction time, 96% ethanol concentration, and three stages extraction. Content means a fraction of yield. These results show that increase in the extraction time, the solvent concentration and the stage numbers of extraction will increase the yield and content of curcumin.

scholarly journals Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Avinash Singh Patel ◽  
Abhijit Kar ◽  
Sukanta Dash ◽  
Sanjaya K. Dash
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Flow Rate ◽  
Extraction Efficiency ◽  
Maximum Yield ◽  
Extraction Time ◽  
Bitter Melon ◽  
Fluid Extraction ◽  
Storage Study ◽  
Β Carotene

AbstractStudy ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO2) flow rates (ranged from 15 to 55 ml/min), temperatures (from 50 to 90 °C), and extraction periods (from 45–225 minutes) were observed on the extraction efficiency of β-carotene. Results showed that extraction pressure (X1) among extraction parameters had the most significant (p < 0.05) effect on extraction efficiency of the β-carotene followed by allowed extraction time (X4), CO2 flow rate (X2) and the temperature of the extraction (X3). The maximum yield of 90.12% of β-carotene from lyophilized enzymatic pretreated ripe bitter melon pericarp was achieved at the pressure of approx. 390 bar, flow rate of 35 mL/min, temperature at 70 °C and extraction time of 190 min, respectively. Based on the accelerated storage study the 70% retention shelf life of the β-carotene into extract was estimated up to 2.27 months at 10 °C and up to 3.21 months at 5 °C.

scholarly journals OPTIMASI EKSTRAKSI DAN PENENTUAN KANDUNGAN KATEKIN DALAM TEH HIJAU (Camellia sinensis) MENGGUNAKAN METODA HPLC

2019 ◽  
Vol 2 (1) ◽  
pp. 43-49
Author(s):  
Sudjatini Sudjatini
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
High Performance ◽  
Dry Weight ◽  
Extraction Process ◽  
Boiling Water ◽  
Water Extraction ◽  
Extraction Time ◽  
Pressure Extraction ◽  
Optimum Period

 This research was designed to determine the optimum period extraction time for catechin green tea using boiling water and ethyl acetate as a solubilizing agent, whereas the composition and the content of catechin green tea were measured using High Performance Liquid Chromatography (HPLC). A single factor experiment design was used. The period extraction time of green tea using boiling water and ethyl acetate were 5, 20, 35 and 60 minutes. The proportion of green tea at the extraction process was 1:15 (weight/volume). The extraction process using boiling water was done twice to get the maximum extracted catechin yield. The extracted catechin of green tea were, therefore, compared with epi-catechin as standard solution. The research’s results show that the optimum period for extraction time of green tea using boiling water and ethyl acetate were 35 and 20 minutes, respectively. The extracted green tea using boiling water was 41.39 % of its dry weight, and those extracted green tea contained catechin as high as 60.62-61.78 %. Catechin compound in green tea residues extracted using ethyl acetate was 5.31 % from 58.35 % the total green tea residues. The composition of catechin in the first boiling water extraction, the second boiling water extraction and pressure extraction consisted of epigallocatechin (EGC), epigallocatechin-gallat (EGCG), epicatechin (EC) and epicatechin-gallat (ECG). ECG was only found in green tea residue extracted using ethyl acetate. The total catechin compound in green tea extracted using boiling water and ethyl acetate was 27.73 % of dry weight, consisted of EGC, EGCG, EC and ECG were 14.74, 1.73, 8,35 and 2.91 % of green tea dry weight, respectively

scholarly journals EKSTRAKSI MULTI TAHAP KURKUMIN DARI KUNYIT (Curcuma domestica Valet) MENGGUNAKAN PELARUT ETANOL

2015 ◽  
Vol 4 (3) ◽  
pp. 29-34 ◽  
Author(s):  
Rajian Sobri Rezki ◽  
Dwimas Anggoro ◽  
Siswarni MZ
Keyword(s):  
Extraction Method ◽  
Ethanol Concentration ◽  
Maximum Yield ◽  
Yellow Crystal ◽  
Extraction Time ◽  
Solvent Concentration ◽  
Orange Yellow ◽  
Three Stages ◽  
Two Stages ◽  
Efficiency Rate

Curcumin is a pigment in Curcuma domestica Valet, which has an orange-yellow crystal appearance, and commonly being used as a colouring agent. Extraction method which is used in extracting curcumin from Curcuma domestica Valet is one stage extraction. This research studied a multi stages extraction of curcumin from Curcuma domestica Valet. The purposes of this research is to find the best condition in extracting curcumin using multi stages extraction method, to increase the efficiency rate in curcumin extraction. This research used ethanol as a solvent, and effect from variables such as extraction time, solvent concentration, and number of extraction stages are observed. Extraction times are 60 minutes, 120 minutes, and 180 minutes. Variations of ethanol concentration are 50%, 70%, and 96%. Stage numbers of extraction are two stages extraction and three stages extraction. Extracts of curcumin are examined with qualitative analysis and quantitative analysis. The best condition of multi stages curcumin extraction is determined based on yield and content of curcumin. The maximum yield obtained in this study was 12% with conditions 180 minutes extraction time, 96% ethanol concentration, and two stages extraction. The highest content of curcumin obtained is 16% with conditions 180 minutes extraction time, 96% ethanol concentration, and three stages extraction. Content means a fraction of yield. These results show that increase in extraction time, solvent concentration, and stage numbers of extraction  will increase the yield and content of curcumin extracted from Curcuma domestica Valet.

scholarly journals Ultrasound-Assisted Extraction of Phenolic Compounds from Moroccan Lavandula stoechas L.: Optimization Using Response Surface Methodology

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yassine Ez zoubi ◽  
Mouhcine Fadil ◽  
Dalila Bousta ◽  
Abdelhakim El Ouali Lalami ◽  
Mohammed Lachkar ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Total Concentration ◽  
Maximum Yield ◽  
Extraction Time ◽  
Plant Metabolites ◽  
Ultrasound Assisted Extraction ◽  
Secondary Plant Metabolites ◽  
Solid Ratio ◽  
Lavandula Stoechas

Secondary plant metabolites, in particular phenolic compounds, are characterized by great diversity in the plant kingdom and are widely used in the medical and industrial fields. The extraction of these compounds represents a critical step, and the differences in extraction conditions strongly affect the yields and the total concentrations of polyphenols in the extracts. In this way, the objective of the present study was to optimize the extraction parameters of the polyphenols from Lavandula stoechas using the extraction technique assisted by ultrasound. Yield and the total concentration of polyphenols have been optimized, taking into account three variables, the extraction time (min), the ethanol concentration (%), and the solvent/extract ratio (ml/g). The optimum extraction yield (31.88%) was obtained by ensuring the following parameters: an ethanol concentration of 40%, a liquid/solid ratio of 30 ml/g, and a time processing of 32.62 min. The maximum concentration of total polyphenols (190.14 mg gallic acid equivalents (GAE)/g) was obtained after 21.5 min of extraction, with a liquid/solid ratio of 30 ml/g and a concentration of ethanol at 40%. In order to obtain the maximum yield (24.9%) and the total concentration of polyphenols (190.14 mg GAE/g) simultaneously, the following parameters must be adjusted: an extraction time of 21.5 min, a liquid/solid ratio of 30 ml/g, and a concentration of ethanol at 40%. The experimental values of the yield and the total concentration of the polyphenols were in good agreement with the predicted values, which suggests that the ultrasonic extraction model adopted in this study is validated.

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