Development of a Functional Beverage Formulation with High Protein Content, Inulin and Stevia

A beverage formulation with proteins of high quality, inulin, Stevia and C vitamin has been prepared. The protein source was bovine plasma which was concentrated and demineralized by a combined membrane methodologies as microfiltration, ultrafiltration and discontinues diafiltration. The use of inulin as protective agent to prevent protein concentrate denaturation during freeze-drying was assayed. The inulin is a versatile food ingredient and it also has health benefits. The powder obtained has improved sensory characteristic and high solubility. Stevia has been added as sweetness, being a low-carbohydrate, low-sugar food alternative. The formulation could be destined as protein supplement with functional properties or for special dietary use.

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Optimised Extraction of Trypsin Inhibitors from Defatted Gac (Momordica cochinchinensis Spreng) Seeds for Production of a Trypsin Inhibitor‐Enriched Freeze Dried Powder

Separations ◽

10.3390/separations6010008 ◽

2019 ◽

Vol 6 (1) ◽

pp. 8 ◽

Cited By ~ 1

Author(s):

Anh V. Le ◽

Sophie E. Parks ◽

Minh H. Nguyen ◽

Paul D. Roach

Keyword(s):

Moisture Content ◽

Freeze Drying ◽

Trypsin Inhibitors ◽

Seed Kernel ◽

Solvent Ratio ◽

Optimal Conditions ◽

High Solubility ◽

High Quality ◽

Freeze Dried ◽

Momordica Cochinchinensis

The seeds of the Gac fruit, Momordica cochinchinensis Spreng, are rich in trypsin inhibitors (TIs) but their optimal extraction and the effects of freeze drying are not established. This study aims to (1) compare aqueous solvents (DI water, 0.1 M NaCl, 0.02 M NaOH and ACN)/water/FA, 25:24:1) for extracting TIs from defatted Gac seed kernel powder, (2) to optimise the extraction in terms of solvent, time and material to solvent ratio and (3) to produce a TI-enriched freeze-dried powder (FD-TIP) with good characteristics. Based on the specific TI activity (TIA), the optimal extraction was 1 h using a ratio of 2.0 g of defatted powder in 30 mL of 0.05 M NaCl. The optimisation improved the TIA and specific TIA by 8% and 13%, respectively. The FD-TIP had a high specific TIA (1.57 ± 0.17 mg trypsin/mg protein), although it also contained saponins (43.6 ± 2.3 mg AE/g) and phenolics (10.5 ± 0.3 mg GAE/g). The FD-TIP was likely stable during storage due to its very low moisture content (0.43 ± 0.08%) and water activity (0.18 ± 0.07) and its ability to be easily reconstituted in water due to its high solubility index (92.4 ± 1.5%). Therefore, the optimal conditions for the extraction of TIs from defatted Gac seed kernel powder followed by freeze drying gave a high quality powder in terms of its highly specific TIA and physical properties.

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Screening of the freeze‐drying protective agent for high quality milk beer yeast ( Kluyveromyces marxianus) and optimization of freeze‐drying process conditions

Journal of Food Processing and Preservation ◽

10.1111/jfpp.16016 ◽

2021 ◽

Author(s):

Liang Wang ◽

Mingying He ◽

Tao Wu ◽

Kangye Yang ◽

Yulian Wang ◽

...

Keyword(s):

Kluyveromyces Marxianus ◽

Freeze Drying ◽

Process Conditions ◽

Protective Agent ◽

Drying Process ◽

High Quality ◽

Beer Yeast

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Some Properties of Platelet Cofactor I Concentrates of Bovine Origin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654516 ◽

1960 ◽

Vol 04 (03) ◽

pp. 342-348 ◽

Cited By ~ 1

Author(s):

William J. Baker ◽

Walter H. Seegers

Keyword(s):

Hydrogen Peroxide ◽

Ascorbic Acid ◽

Magnesium Hydroxide ◽

Barium Sulfate ◽

Freeze Drying ◽

Sodium Sulfite ◽

Barium Carbonate ◽

High Quality ◽

Bovine Plasma ◽

Native Plasma

SummaryPlatelet cofactor I preparations from bovine plasma were studied from the viewpoint of stability in the presence of chemicals selected strategically. The preparations were of high quality having about 86 per cent of the protein as one component by ultracentrifuge analysis. The activity was easily destroyed with hydrogen peroxide, sodium sulfite, phenylhydrazine, formalin, acetone, and by the process of freeze drying. The activity was not lost in the presence of oxygen, ascorbic acid, cysteine, merthiolate, parachloromercuribenzoate or 0.5 per cent phenol. In the native plasma the activity is not adsorbed on barium carbonate, barium sulfate, or magnesium hydroxide. Nor is it destroyed by ether; but, in purified form it is adsorbed on these adsorbants and is destroyed by ether. In the purified preparations the activity was not adsorbed on IRC-50 resin thus making it possible to use this adsorbant to remove any thrombin that might be added to a platelet cofactor I preparation. By this technic it was possible to show that thrombin alone does not easily destroy platelet cofactor I activity.

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Post-Processing Techniques for the Improvement of Liposome Stability

Pharmaceutics ◽

10.3390/pharmaceutics13071023 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1023

Author(s):

Ji Young Yu ◽

Piyanan Chuesiang ◽

Gye Hwa Shin ◽

Hyun Jin Park

Keyword(s):

Freeze Drying ◽

Protective Agent ◽

Post Processing ◽

Additional Process ◽

Spray Freeze Drying ◽

The Stability ◽

Processing Techniques ◽

The Impact ◽

Self Aggregation ◽

Liposome Stability

Liposomes have been utilized as a drug delivery system to increase the bioavailability of drugs and to control the rate of drug release at the target site of action. However, the occurrence of self-aggregation, coalescence, flocculation and the precipitation of aqueous liposomes during formulation or storage can cause degradation of the vesicle structure, leading to the decomposition of liposomes. To increase the stability of liposomes, post-processing techniques have been applied as an additional process to liposomes after formulation to remove water and generate dry liposome particles with a higher stability and greater accessibility for drug administration in comparison with aqueous liposomes. This review covers the effect of these techniques including freeze drying, spray drying and spray freeze drying on the stability, physicochemical properties and drug encapsulation efficiency of dry liposomes. The parameters affecting the properties of liposomes during the drying process are also highlighted in this review. In addition, the impact of using a protective agent to overcome such limitations of each process is thoroughly discussed through various studies.

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Optimization of Protective Agents for The Freeze-Drying of Paenibacillus polymyxa Kp10 as a Potential Biofungicide

Molecules ◽

10.3390/molecules25112618 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2618

Author(s):

Hayatun Syamila Nasran ◽

Hidayat Mohd Yusof ◽

Murni Halim ◽

Nor’Aini Abdul Rahman

Keyword(s):

Fungal Pathogens ◽

Crop Production ◽

Freeze Drying ◽

Fungal Infections ◽

Skim Milk ◽

Paenibacillus Polymyxa ◽

Protective Agent ◽

Protective Agents ◽

Chemical Fungicides

Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively.

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Protein supplement consumption is linked to time spent exercising and high-protein content foods: A multicentric observational study

Heliyon ◽

10.1016/j.heliyon.2019.e01508 ◽

2019 ◽

Vol 5 (4) ◽

pp. e01508

Author(s):

Ewan Thomas ◽

Bettina Karsten ◽

Fatma Nese Sahin ◽

Goktug Ertetik ◽

Francesco Martines ◽

...

Keyword(s):

Observational Study ◽

Protein Content ◽

High Protein ◽

Protein Supplement ◽

High Protein Content

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THE PURIFICATION OF SARINASE FROM BOVINE PLASMA

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y56-112 ◽

1956 ◽

Vol 34 (1) ◽

pp. 1091-1094 ◽

Cited By ~ 1

Author(s):

P. A. Adie

Keyword(s):

Freeze Drying ◽

Electrophoretic Pattern ◽

Unit Weight ◽

Protein Solution ◽

Bovine Plasma

The purification of bovine plasma sarinase has been carried out using alcohol precipitation methods and ultracentrifugation. A protein solution was obtained which was 1250 times more active per unit weight of protein than the original plasma. Sixty per cent of this activity was lost on freeze-drying. The electrophoretic pattern of the preparation showed that the material was apparently homogeneous.

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Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic Acid on Survival of Lactobacillus acidophilus during Freeze-Drying

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.568.279 ◽

2012 ◽

Vol 568 ◽

pp. 279-282

Author(s):

He Chen ◽

Zhen Xing Ma ◽

Guo Wei Shu ◽

Tao Qin

Keyword(s):

Ascorbic Acid ◽

Survival Rate ◽

Lactobacillus Acidophilus ◽

Freeze Drying ◽

Protective Agent ◽

Survival Ratio ◽

Protective Agents ◽

Viable Cells ◽

Before And After

Effect of NaH2PO4, Na2HPO4, MgSO4, and Ascorbic acid on survival of Lactobacillus acidophilus was investigated before and after freeze-drying. Editing different concentrations of protective agents respectively during freeze-drying. After completion of the pilot, the survival ratio of Lactobacillus acidophilus and the number of viable cells were being measured. Results were as follows: the highest survival rate and the largest number of viable cells was Na2HPO4as the protective agent.

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Effect of K2HPO4, KH2PO4, NaHCO3, and Sodium Ascorbate on Survival of Lactobacillus acidophilus during Freeze-Drying

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.531.284 ◽

2012 ◽

Vol 531 ◽

pp. 284-287 ◽

Cited By ~ 2

Author(s):

Zhen Xing Ma ◽

He Chen ◽

Guo Wei Shu ◽

Tao Qin

Keyword(s):

Survival Rate ◽

Lactobacillus Acidophilus ◽

Freeze Drying ◽

Sodium Ascorbate ◽

Protective Agent ◽

Survival Ratio ◽

Protective Agents ◽

Viable Cells ◽

Key Factor

In the present study, the experiments were investigated with the effecting of NaHCO3, KH2PO4, K2HPO4 and Sodium ascorbate on survival of Lactobacillus acidophilus during freeze-drying. Designing different concentrations of protective agents, respectively. The survival ratio of Lactobacillus acidophilus and the number of viable cells will be measured after completing drying. In the process, there were major differences between the different concentrations of protective agents in survival during freeze-drying. As the result, the protective agent was playing a key factor affecting the survival of Lactobacillus acidophilus. At present in the study of the experiments, the highest survival rate was NaHCO3 as the protective agent, and when the protective agent as K2HPO4 the number of viable cells was largest.

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Health Food Supplements (“Health Food”) Highly Nutritious From Chlorella And Oil Catfish (Pangasius hypopthalmus)

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v19i3.14564 ◽

2016 ◽

Vol 19 (3) ◽

pp. 251

Author(s):

Syahrul Syahrul ◽

Dewita Dewita

Keyword(s):

Fatty Acids ◽

Raw Materials ◽

Essential Fatty Acids ◽

Food Supplements ◽

Raw Material ◽

Food Sources ◽

Protein Concentrate ◽

Fish Protein ◽

Health Food ◽

Food Ingredient

The utilization of microalgae as a food ingredient considered effective, because in addition to alternative food sources also contains nutrients chlorella microalgae in particular is very good for health. This microalgae rich in protein (60.5%), fat (11%), carbohydrates (20.1%), water, dietary fiber, vitamins and minerals Besides these microalgae contain pigments (chlorophyll), tocopherol and the active component (antimicrobial and antioxidants). This is what underlies microalgae is very useful to be used as a source of raw materials of health food supplements. Currently the health food supplements have become a necessity for people to maintain their health in order to remain vibrant. This study aims to produce high nutritious health food supplements from raw material chlorella enriched with fish protein concentrate and oil catfish. The method used in the manufacture of high nutritious health food supplement is a method of microencapsulation with different formulations. The results showed that the best formulations based on the profile of amino acids, fatty acids and standards AAE per day especially essential fatty acids oleic and linoleic is formulation B (chlorella 2%, 1% fish oil and fish protein concentrate 1%.

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