STUDY ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF  ALPINIA KWANGSIENSIS T. L. WU AND S. J. CHEN COLLECTED IN VIETNAM

Three compounds named ester methyl-trans-p-coumarate (1), 7-hydroxy-6-methoxycoumarin (2), and (+)-gallocatechin (3) have been isolated from the ethyl acetate fraction of methanol extract of Alpinia kwangsiensis roots collected in Thai Nguyen province. Their structures were elucidated on the basis of spectroscopic data and by comparison with their spectral data reported in literature. This is the first isolation of three compounds from this species. The ethyl acetate fraction was found to be active against bacterias as Staphylococcus aureus and Bacillus subtilis with the IC50 values ranging at 74.65 mg/ml and 80.54 mg/ml, respectively. This fraction also showed antioxidant activity through DPPH test with the EC50 value 87.98 mg/ml.

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Penentuan Aktivitas Antioksidan Berdasarkan Nilai IC50 Ekstrak Metanol dan Fraksi Hasil Partisinya pada Kulit Biji Pinang Yaki (Areca vestiaria Giseke)

Jurnal MIPA ◽

10.35799/jm.3.2.2014.6002 ◽

2014 ◽

Vol 3 (2) ◽

pp. 149

Author(s):

Filbert . ◽

Harry S. J. Koleangan ◽

Max R. J. Runtuwene ◽

Vanda S. Kamu

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Ic50 Values

Tujuan dari penelitian ini adalah untuk menentukan nilai IC50 ekstrak metanol dan hasil partisinya dari ekstrak Areca vestiaria Giseke. Penelitian dimulai dengan proses maserasi menggunakan pelarut metanol, kemudian dievaporasi pada suhu 40oC dan menghasilkan 27,3 g ekstrak pekat dari 330,2 g sampel. Selanjutnya, ekstrak metanol diuji aktivitas antioksidannya dan diperoleh nilai IC50 sebesar 8,3 ppm.. Ekstrak kemudian dipartisi dengan pelarut n-heksana, etil asetat dan air dan dievaporasi. Seluruh fraksi kemudian diuji aktivitas antioksidannya dan fraksi etil asetat menunjukkan aktivitas antioksidan yang paling baik dengan nilai IC50 sebesar 10,9 ppm.The purposes of this research were to determine the IC50 values from methanol extract and its extract partition of extract Areca vestiaria Giseke. The research was started with maceration process using methanol as a solvent, then evaporated at 40oC which produce 27,3 g extract from 330,2 g sample. After that, methanol extract was tested its antioxidant activity and the result of IC50 values was 8,3 ppm. And then, the extract was partitioned with n-hexane, ethyl acetate and water and evaporated. All of the fraction was then tested its antioxidant activity. Ethyl acetate fraction showed the best antioxidant activity with IC50 values of 10,9 ppm.

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Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽

10.3390/molecules26092555 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2555

Author(s):

Sang Koo Park ◽

Yoon Kyung Lee

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Scavenging Activity ◽

Polyphenol Content ◽

Abts Assay ◽

Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

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Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v15i1.2820 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Irfan Zamzani ◽

Nita Triadisti

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Methanol Extract ◽

Oxidation Process ◽

Antioxidant Activities ◽

Alternative Source ◽

Dpph Method ◽

Indigenous Plant ◽

Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15724 ◽

2017 ◽

Vol 10 (2) ◽

pp. 343 ◽

Cited By ~ 4

Author(s):

Resmi Mustarichie

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

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Study on biological activities of extracted fractions from Typhonium flagelliforme (Lodd.) Blume

VNU Journal of Science Natural Sciences and Technology ◽

10.25073/2588-1140/vnunst.4575 ◽

2017 ◽

Vol 33 (2S) ◽

Cited By ~ 1

Author(s):

Le Quy Thuong ◽

Bach Tuyet Mai ◽

Nguyen Minh Chau ◽

Le Thi Phuong Hoa ◽

Nguyen Quang Huy

Keyword(s):

Ethyl Acetate ◽

Cytotoxic Activity ◽

Biological Activities ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Chemical Compositions ◽

Active Components ◽

Stomach Pain ◽

Ic50 Values

Typhonium flagelliforme is a medicinal plant that has variety of uses. In medicinal traditional T. flagelliforme is used to treatment cough, headache, stomach pain chronic, and tracheitis. Moreover, use fresh bulbs treatment furuncle, the bites of poisonous insects. The active components in T. flagelliforme are flavonoids. In this study, the T. flagelliforme extract was obtained by methanol to determine the chemical composition. Then, The extracts of methanol are extracted with polarization increases gradually solvents such as haxane, dichloromethane and ethyl acetate. Determination of antioxidant activity, cytotoxic activity of extracted fractions. Results obtained showed that the chemical compositions by the qualitative reaction preliminary were identified from T. flagelliforme containing reducing sugars, amino acids, organic acids, flavonoids, alcaloids, sterols. The antioxidant capacity of the ethyl acetate fraction reached 94.76 μg/ml, 10 times higher than the positive control is Quercetin. Cytotoxic activity of the haxane and diclomethane extracted fractions from T. flagelliforme exhibited cytotoxic activity on all three experimental cancers cell lines: KB, HepG2, Lu after 72h of culture with IC50 values range from 92.8 to 107.76 μg/ml. From dichlomethane extracted of T. flagelliforme was purified TF1 as Stigmast-4-en- 3-on.

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Phytochemicals, Antimicrobials and Antioxidants Studies of the Stem Bark Extract from Calophyllum ferrugineum

Scientific Research Journal ◽

10.24191/srj.v17i2.6917 ◽

2020 ◽

Vol 17 (2) ◽

pp. 1

Author(s):

Izzah Afifah Noh ◽

Vivien Yi Mian Jong

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Biological Activities ◽

Stem Bark ◽

Extraction Process ◽

Bark Extract ◽

Chemical Components ◽

Isolation And Purification ◽

Antioxidant Agents ◽

Ic50 Values

Traditionally, Calophyllum genus was used for swollen gums, arthritis, diarrhea, chronic abscess, skin infections, and lesions treatment. C. ferrugineum from Sarawak has least studied about their phytochemical constituents and biological activities. The objectives are to isolate and characterize the chemical components from C. ferrugineum and to determine their antimicrobial and antioxidant activity. The plant stem barks were collected from National Park in Sarawak and underwent extraction process. The extracts underwent isolation and purification processes by using several chromatographic methods. Structural elucidation was achieved by using infrared, MS and NMR spectra. The isolation process from the species has afforded 5 known compounds; isocalanone (1) and 1-hydroxy-7-methoxy-9H-xanthen-9-one (2), lupeol (3), friedelin (4) and diethylene glycol dibenzoate (5). The chloroform and methanol extract showed strong inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with the MIC and MBC values ranging from 225 and 112.5 µg/mL. Isocalanone (1) and the methanol extract of C. ferrugineum showed potent antioxidant activity with the IC50 values of 28 ± 5.23 and 35 ± 3.69 µg/mL compared to ascorbic acid, respectively. The results obtained from this study emphasized the potential of the species as antimicrobial and antioxidant agents.

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AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽

10.33751/ekol.v19i1.1660 ◽

2020 ◽

Vol 19 (1) ◽

pp. 34-38

Author(s):

Siti Warnasih ◽

Diana Widiastuti ◽

Uswatun Hasanah ◽

Laksmi Ambarsari ◽

Purwantiningsih Sugita

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Soxhlet Extraction ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Palm Fruit ◽

Ic50 Value ◽

Dpph Method ◽

Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

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Aktivitas Antioksidan Fraksi Etil Asetat Dari Ekstrak Metanol Sargassum sp.

Journal of Marine Research ◽

10.14710/jmr.v8i1.24326 ◽

2019 ◽

Vol 8 (1) ◽

pp. 41-46

Author(s):

Hafida Salma ◽

Sri Sedjati Sedjati ◽

Ali Ridlo

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Vitamin C ◽

Ethyl Acetate ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Rotary Evaporator ◽

Total Antioxidant

Sargassum sp. adalah salah satu jenis rumput laut paling banyak di Indonesia dan memiliki manfaat sebagai antioksidan, obat penyakit jantung, stroke, dll. Penelitian ini bertujuan untuk menentukan kandungan antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. Sampel diambil dari Pantai Sundak, Gunung Kidul, Yogyakarta. Metode yang digunakan adalah eksploratif diskriptif. Sampel dikeringkan dalam suhu ruangan selama 7 hari dan dimaserasi dengan pelarut metanol, lalu dievaporasi dengan rotary evaporator. Ekstrak metanol di fraksinasi menggunakan etil asetat. Pengujian aktivitas antioksidan dilakukan dengan menggunakan 2 metode, yaitu metode penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Vitamin C digunakan digunakan sebagai standar penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Kadar total fenolat diuji menggunakan larutan Folin-Ciocalteu dengan standar asam galat. Hasil penelitian menunjukkan bahwa kadar total fenolat fraksi etil asetat Sargassum sp. sebesar 64,42 mg GAE/g sampel. Aktivitas antioksidan dengan metode penangkapan radikal bebas DPPH memiliki nilai IC50 1.289 ppm, vitamin C memiliki IC50 sebesar 122,71 ppm, sedangkan total kapasitas antioksidan adalah 39,52 mg AAE/g sampel. Kesimpulannya, yaitu kandungan aktivitas antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. dengan metode penangkapan radikal bebas DPPH diduga sangat lemah dan kandungan aktivitas antioksidan dengan menggunakan metode fosfomolibdat diduga tinggi. Sargassum sp. is one of the many types of seaweed in Indonesia and has benefits as an antioxidant, a drug for heart disease, stroke, etc. This study aims to determine the antioxidant content of ethyl acetate fraction of methanol extract of Sargassum sp. Samples were taken from Sundak, Gunung Kidul, Yogyakarta. The method used is descriptive explorative. Samples were dried at room temperature for 7 days and macerated with methanol, then evaporated at a rotary evaporator. The methanol extract fractionation using ethyl acetate. Testing the antioxidant activity using two methods, the method of catching free radicals DPPH and total antioxidant capacity fosfomolibdat. Vitamin C is used as a standard arrest DPPH free radical and total antioxidant capacity fosfomolibdat. Levels of total phenolics were tested using the Folin-Ciocalteu solution with gallic acid standard. The results showed that levels of total phenolic fraction of ethyl acetate Sargassum sp. amounting to 64,42 mg GAE/g sample. The antioxidant activity with catching free radicals DPPH methods have IC50 values 1.289 ppm, vitamin C has an IC50 of 122,71 ppm, while the total antioxidant capacity was 39,52 mg AAE/g sample. In conclusion, the content of the antioxidant activity of ethyl acetate fraction of methanol extract of Sargassum sp. with catching free radicals DPPH methods allegedly extremely weak and the content of the antioxidant activity using methods fosfomolibdat predictably high.

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Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma spinosum Segar dari Perairan Pulau Nain Sulawesi Utara

JURNAL ILMIAH SAINS ◽

10.35799/jis.21.1.2021.33881 ◽

2021 ◽

Vol 21 (1) ◽

pp. 91

Author(s):

Lena Jeane Damongilala ◽

Fitje Losung ◽

Verly Dotulong

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Inhibition Zone ◽

Ethyl Acetate Fraction ◽

Disc Diffusion ◽

Butanol Fraction ◽

Inhibition Zones

Penelitian terhadap ekstrak metanol, fraksi etil asetat, dan fraksi butanol dari rumput laut Eucheuma spinosum menggunakan metode Disc Diffusion Test dilakukan untuk mengetahui peranannya sebagai antibakteri Escherichia coli ATCC 25922, dan Staphylococcus aureus ATCC 6538. Sebagai kontrol digunakan ekstrak (negatif) pelarut metanol, dan Amoxicilin. Konsentrasi sampel uji ditetapkan 10.000 ppm. Hasil pengujian menunjukkan adanya aktivitas antibakteri yang ditandai dengan terbentuknya zona hambat terhadap bakteri Escherichia coli ATCC 25922. Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi butanol, dan fraksi etil asetat, ialah : 6,98 mm, 7,85 mm, dan 7,88 mm. Nilai zona hambat tersebut, relatif sama untuk setiap sampel uji. Sampel uji juga menunjukkan terdapat aktivitas antibakteri, ditandai dengan terbentuknya zona hambat terhadap bakteri Staphylococcus aureus ATCC 6538. Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi butanol, fraksi etil asetat, dan kontrol negatif pelarut metanol, ialah : 7,42 mm, 14.40 mm, 7,90 mm, dan 6,75 mm. Zona hambat fraksi butanol lebih tinggi dibanding yang lain, ini menunjukkan bahwa aktivitas antibakteri Staphylococcus aureus pada fraksi butanol lebih tinggi dibanding fraksi lainnya.Kata kunci: Antibakteri; Eucheuma spinosum; Escherichia coli; Staphylococcus aureus Antibacteria Activities of Extract Sea Algae Eucheuma spinosum Fresh from Nain Island Waters North Sulawesi ABSTRACTA research on antibacterial activity of methanol extract, ethyl acetate, and butanol fraction from Eucheuma spinosum seaweed using the disc diffusion method to know the role as antibacterial activity was tested against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. Methanol was used as negative control while amoxicillin was used as positive control. The concentration of the test sample was set at 10,000 ppm. The antibacterial activity was indicated by the formation of an inhibition zone against the tested microorganisms. The inhibition zones against E.coli for methanol extract, butanol fraction, and ethyl acetate fraction were 6.98 mm, 7.85 mm, and 7.88 mm, respectively. The value of the zone of inhibition is relatively the same for each sample. In addition, the inhibition zones of methanol extract, butanol and ethyl acetate fraction against Staphylococcus aureus were 7.42 mm, 14.40 mm, 7.90 mm, and 6.75 mm, respectively. The inhibition zone of the butanol fraction is higher than the others, this indicated that the antibacterial activity for butanol fraction is higher than other fractions. Keywords: Antibacterial activity; Eucheuma spinosum; Escherichia coli; Staphylococcus aureus

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QUANTIFICATION OF TOTAL PHENOLS AND ANTIOXIDANT ACTIVITY IN CLEMATIS ERECTA AERIAL PARTS

INDIAN DRUGS ◽

10.53879/id.53.12.10665 ◽

2016 ◽

Vol 53 (12) ◽

pp. 52-54

Author(s):

R. Chawla ◽

◽

D. Kumar ◽

K Rana ◽

H. Joshi ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Methanol Extract ◽

Antioxidant Properties ◽

Ethyl Acetate Fraction ◽

Total Phenols ◽

Standard Drug ◽

Butanol Fraction ◽

Aerial Parts

The present investigation was planned to estimate total phenols and antioxidant activity in Clematis erecta aerial parts. Properly identified powdered plant was successively extracted in Soxhlet apparatus with solvents in order of increasing polarity viz., petroleum ether, chloroform, methanol and water. Ethyl acetate fraction and 1-butanol fraction of methanol extract were prepared using standardized procedure. Quantification of phenols was done on the basis of standard curve of gallic acid using standardized procedures. In vitro antioxidant activity was evaluated using DPPH assay in comparison to standard drug rutin. Maximum phenols content and antioxidant activity was found in ethyl acetate fraction followed by methanol extract and 1-butanol fraction, whereas negligible phenols content and antioxidant activity was observed in petroleum ether, chloroform and water extracts. The available literature reveals that polyphenols have been reported to possess antioxidant properties. Finally, it can be concluded that these polyphenols may be responsible for antioxidant properties.

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