Flavon Compound from The Ethyl Acetate Extract of The Stem of  Supit  (Tetracera indica Merr.)

Tetracera indica Merr. in Musi Banyuasin, is one of the traditional medicine used by the community for the treatment of kidney stone disease and gout, but this claim is not recorded in the treatment of kidney stones and gout in Indonesia. In this study, isolation of antioxidant compound from ethyl acetate extracts of supit (Tetracera indica) was done. The isolation was carried out through step gradient polarity extraction, and separated and purified by chromatography technique. The determination of the structure of the isolated compound was performed by spectroscopy method including UV, IR, and NMR 1D and 2D, and antoxidant activity was determined by DPPH method. An active antioxidant compound was isolated from ethyl acetate extract in form of yellow solid (15 mg). Based on spectroscopic analysis the isolated compound was 5,8-dihydroxy-7-methoxyflavone. The compound showed strong antioxidant activity (IC50 8.25 μg/mL) higher than standard ascorbic acid (IC50 11.3 μg/mL). This data concluded that efficacy of supit (Tetracera indica) for the treatment related to antioxidant activity (uric acid) is proven by the identification of one antioxidant compound of this plant.

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SKRINING SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETILASETAT DAUN WEDUSAN (Eupatorium odoratum)

Molekul ◽

10.20884/1.jm.2009.4.2.67 ◽

2009 ◽

Vol 4 (2) ◽

pp. 94

Author(s):

Purwati Purwati ◽

Undri Rastuti

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Research Method ◽

Oxidation Process ◽

Ethyl Acetate Extract ◽

Natural Process ◽

Inhibition Activity ◽

Octadecanoic Acid ◽

Fat Quality

Oxidation process is a natural process which always occurs in fat. The process affects and decreases the fat quality. Oxidation in fat can be hampered by the addition of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know the possibility of wedusan leaf as an antioxidant. Hence, the aims of the research were to determine the antioxidant activity of ethyl acetate extract of wedusan leaf using TBA method, and to compare the antioxidant activity of wedusan leaf and that of BHT. The research method consisted of sample preparation, extraction, and determination of antioxidant activity using TBA method. Wedusan leaf was extracted by maceration using n-hexane and ethyl acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate extract was 13.12 gram. Based on qualitative screening on secondary metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS indicated that ethyl acetate extract contained methyl heptadecanoic, methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v) of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05% (w/v) of ethyl acetate extract.

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Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.13662 ◽

2020 ◽

Vol 6 (3) ◽

pp. 198-205

Author(s):

Nur Faidah ◽

Nurhaeni ◽

Ahmad Ridhay ◽

Jusman ◽

Abd. Rahman Razak ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Allium Cepa ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Root Extract ◽

Polar Solvents ◽

Ic50 Value ◽

Dpph Method ◽

Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES BY DPPH RADICAL SCAVENGER ACTIVITY OF RUELLIA BRITTONIANA FLOWER

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33531 ◽

2019 ◽

pp. 24-28

Author(s):

NADZILA ANINDYA TEJAPUTRI ◽

ADE ARSIANTI ◽

FONA QORINA ◽

QOTRUNNADA FITHROTUNNISA

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Properties ◽

Chemical Components ◽

Phytochemical Analysis ◽

Radical Scavenger Activity ◽

Scavenger Activity ◽

Dpph Method ◽

Tlc Analysis

Objective: The genus Ruellia has been widely used in traditional and Ayurvedic medicine as an antioxidant. This study seeks to examine the antioxidant activity of the species Ruellia brittoniana. Methods: In this study, Ruellia brittoniana flowers were acquired from Depok, West Java, Indonesia. The flowers were cleaned and ground to form a powder, then dissolved in hexane, ethanol and ethyl acetate solvents. These three extracts were then tested for phytochemicals and thin layer chromatography (TLC) analysis. Ethanol and ethyl acetate extracts were also analyzed for antioxidants using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: Phytochemical results from the three extracts proved that Ruellia brittoniana contains flavonoids, alkaloids, tannins, glycosides and triterpenes. These results are comparable to the results from TLC analysis, which showed the samples contained 4–5 chemical components. Furthermore, the best antioxidant activity resulted from the ethyl acetate extract of the Ruellia brittoniana flower with an IC50 value of 68.42 ppm. Conclusion: An ethyl acetate extract from the Ruellia brittoniana flower can be used as a natural source of additional antioxidants.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.51 ◽

2021 ◽

Vol 3 (2) ◽

pp. 53-61

Author(s):

Angga Crystal Loasana Yami ◽

Irmanida Batubara ◽

Kholis Abdurachim Audah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Vibrio Cholerae ◽

Pathogenic Bacteria ◽

Ethyl Acetate Extract ◽

Antibacterial Activities ◽

Diffusion Method ◽

Ic50 Value ◽

Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.433 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41-47

Author(s):

Poppy Anjelisa Zaitun Hasibuan ◽

Mardiana

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Ethanol Extract ◽

Positive Control ◽

Hexane Extract ◽

Phytochemical Screening ◽

Ic50 Value ◽

Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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Antioxidant Activity of Syzygium samarangense L. and Their Endophytic Fungi

Molekul ◽

10.20884/1.jm.2019.14.1.503 ◽

2019 ◽

Vol 14 (1) ◽

pp. 48 ◽

Cited By ~ 2

Author(s):

Budiono Budiono ◽

Elfita Elfita ◽

Muharni Muharni ◽

Heni Yohandini ◽

Hary Widjajanti

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Endophytic Fungi ◽

Chemical Structure ◽

Ethyl Acetate Fraction ◽

Spectroscopy Analysis ◽

Potato Dextrose Broth ◽

Dpph Method ◽

Local Residents ◽

Isolated Compound

Leaves of jambu air (Syzygium samarangense L.) has been used by local residents as medicine for various diseases caused by free radical agents in human’s body. This study aims to find a new source of antioxidants from medicinal plants and their endophytic fungi Syzygium samarangense leaves was fractionated by maceration method using gradient solvent i.e. n-hexane, ethyl acetate, and methanol. The antioxidant activity of the leaf fractions was determined using 1,1diphenyl-2-picryl hydrazyl (DPPH) method. The chemical compound was isolated from active fraction by chromatographic techniques and their chemical structure was identified using spectroscopy techniques. The endophytic fungi was isolated from S. samarangense leaves and continued to cultivation in potato-dextrose broth (PDB) medium for four weeks. The medium was extracted using ethyl acetate and determined its antioxidant activity. Fungi’s isolate with highest activity was analyzed to identify its molecular. Ethyl acetate fraction of S. samarangense leaves showed highest antioxidant activity. Spectroscopy analysis result concluded the isolated compound is 5,7-dihydroxy-6,8-dimethyl flavanone. Four endophytic fungi had been isolated form S. samarangense leaves. Antioxidant activity test showed that ethyl acetate extract of endophytic fungi BJA-1 has the highest value. Molecular identification of BJA-1 shows high homology with Lasiodiplodia venezuelensis strain CBS 129753.

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PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

Jurnal Ilmiah As-Syifaa ◽

10.33096/jifa.v11i1.501 ◽

2019 ◽

Vol 11 (1) ◽

pp. 09-16

Author(s):

Epi Supri Wardi ◽

Zulkarni R Zulkarni R ◽

Desy Nurdianti

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Phenolic Content ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Hexane Extract ◽

Total Phenolic ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

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Isolation and Structure Determination of Antioxidants Active Compounds from Ethyl Acetate Extract of Heartwood Namnam (Cynometra cauliflora L.)

Jurnal Kimia Terapan Indonesia ◽

10.14203/jkti.v19i1.325 ◽

2017 ◽

Vol 19 (1) ◽

pp. 11-17

Author(s):

Dede Sukandar ◽

Siti Nurbayti ◽

Tarso Rudiana ◽

Tsalats Wahyul Husna

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Aromatic Ring ◽

1H Nmr ◽

Ethyl Acetate Extract ◽

Active Compounds ◽

Chromophore Group ◽

Ic50 Value ◽

High Antioxidant Activity

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring at band I (λmax 330.22 nm), chromophore group of alkene (C=C) at band II (λmax 268.67 nm) and functional groups such as O−H (3343.91 cm-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1), C−O (1269.89) and C−H ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at δH 6.86 ppm (1H, s), C-6 at δH 5.95 ppm (1H, d, J=1.95 Hz), C-8 at δH 6.25 ppm (1H, d, J= 1.95 Hz), C-2’ and C-6’ at δH 7.03 ppm (2H, d, J=7.87 Hz), C-3’ and C-5’ at δH 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC50 value of 5.18 ± 0.014 ppm.

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ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.)

CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) ◽

10.24843/ck.2017.v05.i01.p04 ◽

2017 ◽

Vol 5 (1) ◽

pp. 26

Author(s):

Ni Wayan Rika Kumara Dewi ◽

I Wayan Gunawan ◽

Ni Made Puspawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Infrared Spectra ◽

Ethyl Acetate Extract ◽

Total Flavonoid ◽

Separation And Purification ◽

Ic50 Value ◽

Ethyl Acetate Extracts ◽

In Vitro Antioxidant Activity

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol. ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.

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Phytochemical Investigation and Antioxidant Activity of Globularia alypum L.

Molecules ◽

10.3390/molecules26030759 ◽

2021 ◽

Vol 26 (3) ◽

pp. 759 ◽

Cited By ~ 1

Author(s):

Fadoua Asraoui ◽

Ayoub Kounnoun ◽

Hafssa El Cadi ◽

Francesco Cacciola ◽

Yassine Oulad El Majdoub ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Properties ◽

Soxhlet Extraction ◽

Chloroform Extract ◽

Total Phenolic ◽

Frap Assay ◽

Globularia Alypum

The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 ± 0.61 µg GAE/mg of extract in comparison with the chloroform extract (18.9 ± 0.48 µg GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 ± 0.55 µg CE/mg of extract) in comparison with the chloroform extract (18.0 ± 0.36 µg CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 ± 3.83 µg/mL and IC50 = 37.0 ± 2.45 µg/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 ± 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.

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