scholarly journals Aktivitas Antioksidan dan Fitokimia dari Ekstrak Etil Asetat Pucuk Idat (Cratoxylum glaucum)

2018 ◽  
Vol 5 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Natural Antioxidant ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidants ◽  
Synthetic Antioxidant ◽  
Phytochemical Content

Neutralizing free radicals can use both synthetic and natural antioxidant compounds. Although synthetic antioxidant compounds are more active, but lately the use of synthetic antioxidants is being reduced because they are reported to have carcinogenic side effects. Natural antioxidant compounds found in plants are phenolic groups such as flavonoids, tannins, xanthones, and anthraquinones. This compound is widely found in the genus Cratoxylum where one of the species is pucuk idat (Cratoxylum glaucum). Pucuk idat are often used by people of Bangka as a flavoring dish and are believed to be traditional medicine to facilitate breastfeeding, tighten skin, treat fever, cough, and diarrhea. The increasing of antioxidants needs and lots efficacy of pucuk idat, hence this study aims to determine the antioxidant activity of ethyl acetate extract Cratoxylum glaucum. The antioxidant test in this study used the DPPH (Diphenylpicrylhydrazyl) method and vitamin C as a positive control. The results of this study indicate that the extract of ethyl acetate Cratoxylum glaucum has strong antioxidant activity with value IC50 32,213 μg / mL. Phytochemical content itself includes hydroquinone phenols (tannins), flavonoids, and steroids. This shows that ethyl acetate extract Cratoxylum glaucum can be used as natural antioxidant.

scholarly journals Formulation and Evaluation of Antioxidant Peel-Off Face Mask Containing Red Dragon Fruit Rind Extract (Hylocereus polyrhizus Haw.)

2020 ◽  
Vol 20 (3) ◽  
pp. 438
Author(s):  
Trasna Arman Jani ◽  
Aliefman Hakim ◽  
Yohanes Juliantoni
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Face Mask ◽  
Hexane Extract ◽  
Product Evaluations ◽  
Drying Time ◽  
Dragon Fruit ◽  
Dispersive Power

Peel-Off face mask is one of the cosmetics that is used to treat skin from free radicals. Peel-off face mask can minimize the effects of free radicals because it contains antioxidant. One of the antioxidant sources is red dragon fruit’s rind (Hylocereuspolyrhizus Haw.). The aim of this study is to obtain Peel-Off face mask of red dragon fruit’s rind extract that contains anthocyanin. Red dragon fruit’s rind was macerated by ethanol 96%, ethyl acetate and n-hexane added citric acid (4:1) solvents. TLC test used Chloroform: ethyl acetate: n-butanol (5:4:1) eluent and sprayed by AlCl3. Antioxidant activity of extract was tested using DPPH (1,1-Diphenyl-2-Picryhidrazyl) method. Peel-Off face mask was made using PVA, HPMC, methyl paraben, propylparaben, propylenglycol, extract, ethanol 96% and aquadest. The rendemen of ethanol 96% extract was 9,476%, ethyl acetate extract was 0,783% and n-hexane extract was 0,631%. The results of TLC test showed yellow spots on the ethyl acetate and ethanol 96% extracts which indicated that extract contained flavonoids (anthocyanin). The results of antioxidant activity test showed that IC50 value of ethanol 96% extract was 189,7422 (AAI=0,2087), ethyl acetate extract was 196,9398 (AAI=0,2011), and n-hexane extract was 385,3664 (AAI=0,1027). The result of the product evaluations showed that all the formulas complied the product requirements such as the organoleptic, homogenity, pH, dispersive power and drying time. Peel-off face mask already meet the product requirements but further research is needed to test the product stability and activity.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

scholarly journals KAJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN TEMBELEKAN (Lantana camara L) BERDASARKAN TINGKAT KEPOLARAN PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (3) ◽  
Author(s):  
Oktavian Mangela ◽  
Ahmad Ridhay ◽  
Musafira Musafira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Antioxidant Compounds ◽  
Ic50 Value

The investigation about the antioxidant activity of tembelekan (Lantana camara L) leaf extract based on level polar solvent . Has been done the aim of this study was to determine the IC50 value and to know the content of antioxidant compounds from tembelekan leaf extract based on the level of polar solvent the extraction of tembelekan leaf was done with maceration method . The result showed that ethyl acetate extract of tembelekan leaf has the highest antioxidant activity with IC50 value was 71,70 ppm. Keywords: Tembelekan ( Lantana Camara L ) , IC50 , Antioxidant , DPPH

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG MAHONI (Swietenia mahagoni Jacq.) MENGGUNAKAN METODE DPPH (1,1 diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 548
Author(s):  
Amanda T. M. Turangan ◽  
Defny S. Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Medicinal Plant ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Oxidation Reactions ◽  
Dpph Method ◽  
Swietenia Mahagoni

Antioxidant are compounds that are able to inactivate the development of the oxidation reactions of the molecules or neutralize free radicals. Mahogany bark (Swietenia mahagoni Jacq.) has properties as a medicinal plant. Through the phytochemical screening, the mahogany bark (Swietenia mahagoni Jacq.) contains antioxidant compounds such as alkaloids, saponins and phenolics. The aim of this study was to determine the presence of antioxsidant activity in the extract of mahogany bark (Swietenia mahagoni Jacq.). using the DPPH method with a concentration of 250 mg / L, 200 mg / L, 150 mg / L and 100 mg / L and Vitamin C p.a as a positive control. Each sample made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The result of the study showed that mahogany bark (Swietenia mahagoni Jacq.) showed antioxidant activity with the percentage of 21,50% at the concentration at 250 mg / LKeywords : Swietenia mahagoni Jacq., Antioxidant, Ethanol, DPPHABSTRAKAntioksidan ialah senyawa yang mampu menginaktivasi berkembangnya reaksi oksidasi molekul lain atau menetralisir radikal bebas. Kulit Batang Mahoni (Swietenia mahagoni Jacq.) memiliki khasiat sebagai tanaman obat. Melalui skrining fitokimia, Swietenia mahagoni Jacq. Mengandung senyawa antioksidan seperti alkaloid, saponin dan fenolik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan didalam ekstrak tanaman Kulit Batang Switenia mahagoni Jacq. menggunakan metode DPPH dengan konsentrasi 250 mg/L, 200 mg/L, 150 mg/L dan 100 mg/L dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak Kulit Batang Swietenia mahagoni Jacq. memiliki aktivitas antioksidan dengan presentase sebesar 21,50% pada konsentrasi 250 mg/L Kata Kunci : Swietenia mahagoni Jacq., Antioksidan, Etanol, DPPH

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals Crystal Guava (Psidium guajava L. “Crystal”): Evaluation of In Vitro Antioxidant Capacities and Phytochemical Content

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Rika Hartati ◽  
Hashifah I. Nadifan ◽  
Irda Fidrianny
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Activity Index ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Positive Correlation ◽  
Phytochemical Content ◽  
Fruit Extracts

Free radicals can cause many diseases, such as cancer. Antioxidant is a compound that could scavenge free radicals. One of the natural antioxidants is guava. The goals of this research were to investigate the antioxidant activity of leaves and fruit of crystal guava by determining the value of the Antioxidant Activity Index (AAI) using DPPH, CUPRAC, and FRAP; evaluate the total phenolic content (TPC) and total flavonoid content (TFC); analyse the correlation between the TPC and TFC with AAI DPPH, CUPRAC, and FRAP, and analyse the correlation between the 3 methods. Extraction was performed by the reflux method using n-hexane, ethyl acetate, and ethanol. Determination of AAI DPPH, CUPRAC, FRAP, the TPC, and the TFC was performed by UV-visible spectrophotometry. The correlation of the TPC and TFC with AAI DPPH, CUPRAC, and FRAP and, also, the correlation of the 3 methods were investigated by Pearson’s method. The antioxidant activity of leaves and fruit extracts of crystal guava showed AAI DPPH in the range of 0.33–56.46, CUPRAC 0.20–7.31, and FRAP 1.65–59.89. The highest TPC was given by ethanol leaf extracts (49.55 ± 1.45 g GAE/100 g), while the highest TFC was for n-hexane leaf extracts (9.68 ± 0.210 g QE/100 g). The TPC of leaves extract had a significantly positive correlation with AAI DPPH, CUPRAC, and FRAP. AAI DPPH, AAI CUPRAC, and AAI FRAP of leaves and fruit extract of crystal guava showed a significantly positive correlation. In general, leaves extract had strong antioxidant activity by the three methods. For the highest antioxidant activity, ethanol was the best solvent for extraction leaves and ethyl acetate for extraction fruit of crystal guava. The TPC in leaves extract contributed to the antioxidant activity by DPPH, CUPRAC, and FRAP methods. The Antioxidant activity of leaves and fruit extracts of crystal guava was linear by the three methods.

scholarly journals Aktivitas Penghambatan α-glukosidase dan Peredaman Radikal Bebas Ekstrak Kapang Endofit yang Diisolasi dari Rimpang Kunyit

2019 ◽  
Vol 29 (3) ◽  
pp. 189-196
Author(s):  
Eris Septiana ◽  
Bustanussalam Bustanussalam ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Blood Sugar ◽  
Endophytic Fungi ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Ic50 Values ◽  
Sugar Levels

Abstract Diabetes mellitus is one of the metabolic disorders that causes an increase in blood sugar levels above normal the normal threshold. The case of diabetes is usually accompanied by an increase in free radicals in the patient’s body. In Indonesia, one of the plants traditionally used to treat high blood sugar levels and contains antioxidant compounds is turmeric. The use of endophytic fungi from medicinal plants as a source of active compounds is widely carried out. Therefore this research aims to determine the antidiabetic and antioxidant activity of the extract of turmeric endophytic fungi from Bogor in vitro. The antidiabetic test used the method of inhibiting of α-glucosidase enzymes, while the antioxidant test used the method of reducing free radicals 2,2-Diphenyl-1-picrylhydrazyl (DPPH). The test results show that the five endophytic mold ethyl acetate extracts have the ability to inhibit the α-glukosidase enzyme and antioxidant activity. Ethyl acetate extract isolate Bo.Ci.Cl.R5 was the most active in α-the the inhibitory activity of a glucosidase enzyme activity and free radical reduction with IC50 values of 336.22 μg/mL and 91.70 μg/mL respectively. Therefore extract of endophytic fungi isolates Bo.Ci.Cl.R5 isolate has the potential to be developed as an alternative raw material for antidiabetic drugs. Abstrak Diabetes melitus merupakan salah satu kerusakan metabolisme tubuh yang menyebabkan naiknya kadar gula dalam darah di atas ambang batas normal. Kasus diabetes biasanya diiringi oleh meningkatnya radikal bebas dalam tubuh penderita. Di Indonesia, salah satu tanaman yang secara tradisional digunakan untuk mengobati kadar gula darah yang tinggi dan mengandung senyawa antioksidan ialah tanaman kunyit. Pemanfaatan kapang endofit asal tanaman berkhasiat obat sebagai sumber senyawa aktif banyak dilakukan. Oleh karena itu penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan antioksidan dari ekstrak kapang endofit rimpang kunyit asal Bogor secara in vitro. Uji antidiabetes menggunakan metode penghambatan aktivitas enzim α-glukosidase, sedangkan uji antioksidan menggunakan metode peredaman radikal bebas 2,2-Diphenyl-1-picrylhydrazyl (DPPH). Hasil pengujian menunjukkan bahwa kelima ekstrak etil asetat kapang endofit memiliki kemampuan dalam menghambat enzim α-glukosidase dan memiliki aktivitas antioksidan. Ekstrak etil asetat isolat Bo.Ci.Cl.R5 merupakan yang paling aktif pada uji aktivitas penghambatan aktivitas enzim α-glukosidase dan peredaman radikal bebas dengan nilai IC50 masing-masing uji sebesar 336,22 μg/mL dan 91,70 μg/mL. Oleh karena itu ekstrak isolat kapang endofit Bo.Ci.Cl.R5 berpotensi dikembangkan sebagai alternatif bahan baku obat antidiabetes.

scholarly journals An Evaluation of The Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 102-110
Author(s):  
Masendra Masendra ◽  
Brandon Aristo Verick Purba Purba ◽  
Ganis Lukmandaru
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Compounds ◽  
Potential Source ◽  
Antioxidant Agents ◽  
Pinus Merkusii ◽  
Ethyl Acetate Extracts

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

scholarly journals POTENSI ANTIOKSIDAN METABOLIT SEKUNDER FRAKSI ETIL ASETAT DARI KULIT BATANG EBONI (Diospyros celebica Bakh.)

KOVALEN ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 155-165
Author(s):  
Vivi Sasmita ◽  
Ahmad Ridhay ◽  
Hardi Ys
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Spectral Data ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Wave Number ◽  
Antioxidant Compounds ◽  
Ic50 Value

Research on the antioxidant potential of the secondary metabolite compound, ethyl acetate from ebony bark (Diospyros celebica Bakh.) has been conducted. This study aims to characterize and determine the antioxidant activity of secondary metabolites of ethyl acetate fraction from ebony bark. Ethyl acetate extract was fractionated using Liquid Vacum Chromatography (KVC) and Gravity Column Chromatography (KKG). The isolated compounds were characterized using UV-Vis and IR spectrophotometers. Data on maximum absorption of λmax 273 nm from UV-Vis analysis showed the presence of chromophore phenol. IR spectral data showed several specific peaks, among others, at wave number (ʋ) of 3425.58 cm-1 indicating the presence of –OH group, C-H aliphatic bond at ʋ of  2924.09 cm-1  and 2854.65 cm-1, and the peak at ʋ of 1604.77 -1465.90 cm-1 and 3078.39 cm-1 indicated the aromatic group, reinforcing the assumption that the isolates produced were phenolic compounds. IC50 value of 75.47 µg/mL from antioxidant activity testing showed that the isolates obtained were antioxidant compounds with strong antioxidant categories. Keywords: antioxidant, Diospyros, ebony, phenolic, isolation

scholarly journals Extraction of Bioactive Compounds as Natural Antioxidants from Fresh Spirulina platensis using Different Solvents

2015 ◽  
Vol 18 (1) ◽  
Author(s):  
Fiya Firdiyani ◽  
Tri Winarni Agustini ◽  
Widodo Farid Ma'ruf
Keyword(s):  
Ethyl Acetate ◽  
Spirulina Platensis ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Natural Antioxidant ◽  
Synthetic Antioxidants ◽  
Effect Of Solvent ◽  
Ic50 Values ◽  
Keywords Antioxidants ◽  
Positive Results

Spirulina platensis is a bioactive-rich microalga of great potential as a source of natural antioxidant. Natural antioxidants are increasing in demand, because it is considered as more safe than synthetic antioxidants. The objective of this study was to determine the effect of solvent on the potential of natural antioxidant from fresh S. platensis. The antioxidant compound was extracted using either acetone or ethyl acetate by reflux method. The yield of extractions were 1.86% and 3.07% for aceton and ethyl acetate, respectively. Fresh S. platensis extracts were considered as potential antioxidant source with IC50 values 65.89 ppm, and 76.36 ppm when using acetone and ethyl acetate, respectivetly, even though it were still lower compare to the IC50 of positive control quercetin i.e 21.64 ppm. Phytochemical test showed positive results of phenolic compound, triterpenoids, steroid, flavanoid, and saponin. aw value of Spirulina platensis was 0.61 and 0.81 for aceton and ethyl acetate, respectively. It was concluded that different solvent affected the antioxidant activity of S. platensis extract. Keywords: Antioxidants, aceton, ethyl acetate, fresh Spirulina platensis

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