scholarly journals Profil Glukosa Darah, Lipid dan Visualisasi Pulau Langerhans sebagai Imunoreaktor Insulin dan Glukagon pada Pankreas Tikus (rattus norvegicus) Obesitas Menggunakan Teknik Imunohistokimia

2017 ◽  
Vol 1 (1) ◽  
pp. 24
Author(s):  
Dela Ria Nesti ◽  
Ahmad Baidlowi
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Cholesterol Level ◽  
Glucose Level ◽  
Blood Lipid ◽  
Blood Cholesterol ◽  
Blood Capillary ◽  
Blood Lipid Profile ◽  
Lipid Metabolism Disorder

Obesity is not concerned as specific congenital disorder or derived lipid metabolism disorder recently, but more to bod yinability to handle the increase of nutrition intake caused by behavioral changes. Langerhans Islet is a section in the pancreas which play crucial role in producing and regulating metabolism hormones, insulin and glucagon. This research was conducted to observe blood glucose and lipid profile and to visualize Langerhans Islet in rat pancreas which were induced obesity and compared it with normal ones. Ten of male non-genetic obese four weeks old Winstar rats were divided into two groups, normal (K) and induced with obesity (O). Blood plasma samples were collected to determine blood glucose and blood lipid profile. Pancreas (regio splenic) samples were collected and fixated with Bouin solution, blocked by paraffin and cut with 5 μm thickness. Histology preparats were stained with Hematoxylin-Eosin and Immunohistochemistry (IHC) is determined with Labeled Sterptavidin-Biotin (LSAB) method. Staining is act as visual detector with will be analyzed descriptively and quantitatively. The data of Obesity group showed that blood glucose level is 88.59 ± 1.77 mg/dl, blood cholesterol level is 160.62±3.52 mg/dl, triglycerides level is 98.61±2.66 mg/dl, HDL level is 44.68±2.25 mg/dl and LDL level is 67.51±1.81. Pancreas’s topography in obese group spread into three regions, which are: gastrium, duodenum and lien. Langerhans Islet morphology shaped oval and polygonal also surrounded by fine filaments and blood capillary. Insulin and glucagon immunoreactive cell’s morphology in obesity group shaped irregular polygonal with round/oval nucleus placed in central/peripheral. Langerhans Islet’s in obesity group has diameter (154.38±65.56) μm, circumference (373,51±146,69) μm and volume 3241105.96 μm3. Obesity induced rat showed the increase in cholesterol level, triglycerides level, LDL level, blood glucose level and Langerhans Islet’s diameter, volume and circumference compared to normal ones (P<0.05). Decrease of HDL, topography and morphology of pancreas in obesity induced rat did’t showed significant changes compared to normal ones.

scholarly journals High antioxidant level in cajanus sajan reduces blood glucose level and improves blood lipid profile of rats as diabetes mellitus models

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Yuli Laraeni ◽  
I Gusti Ayu Nyoman Danuyanti ◽  
Ersandhi Resnhaleksmana ◽  
Iswari Pauzi ◽  
I Komang Agusjaya Mataram ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Glucose Level ◽  
Blood Lipid ◽  
Polyol Pathway ◽  
Protein Glycation ◽  
Blood Lipid Profile ◽  
Reduce Blood Glucose ◽  
Reduce Blood Glucose Level

Hyperglycemia in DM was able to increase glucose auto-oxidation, protein glycation, and polyol pathway activation. As a result, it can accelerate the formation of reactive oxygen compounds acting as the main factor causing DM complication. Prior to our evaluation of pigeon bean Tempe with formulations of 25%, 50% and 75%, we tested the number and activity of its antioxidant. Then we substituted it on standard feed for 14 days to find the effects on glucose level and lipid profile. The effects was then compared to that before the intervention. The result showed that the total and activity of antioxidant in pigeon bean Tempe (Cajanus sajan) was 15% and 41.30% respectively. On the other side, pigeon bean Tempe with a formulation of 75% was more able to reduce blood glucose level, total cholesterol, triglycerides, and LDL-cholesterol by 44.08%, 34.38%,35.80%, and 43.22% respectively (p<0.05) if compared to pigeon bean Tempe with formulations of 25% and 50%. Meanwhile pigeon bean Tempe with formulation of 75% provided better effects for an increase of HDL level 56.20% (p<0.05). All test results on Tempe formulation used were compared to that before intervention.

scholarly journals Effect of bitter melon and garlic on blood glucose level and blood cholesterol level in rats in diabetic condition

2018 ◽  
Vol 5 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Rupaly Akhter ◽  
Imrul Hasan Rasel ◽  
Mohammad Saiful Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Dose Rate ◽  
Cholesterol Level ◽  
Glucose Level ◽  
The Body ◽  
Blood Cholesterol ◽  
Bitter Melon ◽  
Blood Cholesterol Level

The aim of this research was designed to investigate the single and combine effect of bitter melon and garlic on blood glucose level and blood cholesterol level in rats in diabetic condition. In this study 60 rats (12 normal rats and 48 alloxan induced diabetic rats) was used for trials. The rats were divided into 5 groups for each trial, each containing 12 individuals as follows: First group was normal control (A), Second group was diabetic control (B), third group was alloxan with bitter melon treated (C). Group D was alloxan and Garlic treated and Group E was alloxan, bitter melon, garlic treated. Then alloxan injection was injected at the dose rate of 100mg/kg body weight intraperitoneally to each rate to induce diabetes in groups B, C, D and E. On 10th day blood glucose level, blood cholesterol level and the body weights were measured for the first time to ensure diabetic induction as well as hypercholesterolemia. Then all the rats of that group were kept for more 21 days for the treatment of hyperglycemia and hypercholesterolemia. During that period on Day 0, 7, 14 and 21st the body weight, blood cholesterol level and blood glucose level were measured. Aqueous extract of bitter melon and garlic were fed at a dose rate of 300mg/kg and 500 mg/kg body weight for 21 days in group C and D respectively and combine in group E. The blood sugar level and blood cholesterol level were reduced in bitter melon, garlic & combine treatment groups compare with untreated group. From these findings it is concluded that the combination of bitter melon and garlic can be used as anti-hyperglycemic and anti- hypercholesterolemic agent. Res. Agric., Livest. Fish.5(3): 359-363, December 2018

scholarly journals PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE

2018 ◽  
Vol 10 (1) ◽  
pp. 71
Author(s):  
Shibu Narayan Jana ◽  
Papiya Mitra Mazumder
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Cholesterol Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Fasting Blood Glucose Level ◽  
Β Cells ◽  
Partial Pancreatectomy ◽  
Swiss Albino Mice ◽  
Diabetes Status

Objective: The present study was aimed at the development of partial pancreatectomy in a murine model for diabetes.Methods: Diabetes model was successfully developed by partial pancreatectomy method. In this study, cyclosporine was used for influenced the glycaemic status. Diabetes status was evaluated by fasting blood glucose level (FBG), lipid profile (by estimation of total cholesterol level and HDL-level), serum amylase and biochemical assay like glutathione estimation.Results: We report here the restoration of euglycaemic status in cyclosporine-induced diabetic in swiss albino mice after 30% pancreatectomy. In this study, Pancreatectomised (partial) group of animals showed a rapid elevation of glycaemic status, starting from 15th post observational d, but the level of significance decreased gradually from 15th (P<0.01) to 60th (P<0.05) d. This was probably due to nesidioblastotic activity which shifted the fasting blood glucose level towards normoglycaemic status with β-cells regeneration. Cyclosporine treated a group of mice showed normoglycaemic status throughout the whole experimental period, but the cholesterol level remained significant (P<0.001) till the end of the experimental d. Gradually decrements in glycaemia of the diabetic pancreatectomised animals demonstrate islets neogenesis occurring after the operative activity, leading to normoglycaemic condition, probably attributed to β-cells proliferation.Conclusion: The biochemical and histopathological evaluations suggest that there is the development of the diabetic model in the pancreatectomized group and diabetes status induced by pancreatectomy is curable to a certain extent due to the regeneration of β-cells.

scholarly journals ASSOCIATION BETWEEN BLOOD LIPID PROFILE AND BLOOD GLUCOSE LEVELS IN MEN WITH CORONARY HEART DISEASE, METABOLIC SYNDROME, AND TYPE 2 DIABETES MELLITUS

2013 ◽  
Vol 12 (5) ◽  
pp. 29-33
Author(s):  
S. A. Matveeva
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Lipoprotein Cholesterol ◽  
Blood Lipid Profile ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aim.To study the associations between blood lipid profile and blood glucose levels in men with coronary heart disease (CHD), stable effort angina (SEA), metabolic syndrome (MS), and Type 2 diabetes mellitus (DM-2).Material and methods.The study included 82 men (mean age 50,5±0,9 years) with CHD, Functional Class I–III SEA, MS, and DM-2. The following lipid profile parameters were assessed: total cholesterol (TCH), triglycerides (TG), low-density lipoprotein cholesterol (LDL–CH), very low-density lipoprotein cholesterol (VLDL–CH), high-density lipoprotein cholesterol (HDL–CH), atherogenic index (AI), and triglyceride index (TGI), together with fasting blood glucose.Results.There were positive (direct) associations between higher levels (>90th percentile) of lipid profile parameters (TCH, TG, LDL–CH, VLDL– CH, HDL–CH, AI, TGI) and blood glucose, as well as between lower levels (≤10th percentile) of lipid profile parameters (TCH, TG, LDL–CH, VLDL– CH, AI, TGI) and blood glucose. At the same time, there were negative (inverse) associations between lower lipid levels (≤10th percentile of TCH, TG, LDL–CH, VLDL–CH, HDL–CH, AI, TGI) and higher glucose levels (>90th percentile), as well as between higher lipid levels (>90th percentile of TCH, TG, LDL–CH, VLDL–CH, HDL–CH, AI, TGI) and lower glucose levels (≤10th percentile).Conclusion.Dyslipidemia and hyperglycemia demonstrate synergetic proatherogenic effects in patients with CHD, SEA, MS, and DM-2, as suggested by significant heterogeneous (direct and inverse) associations between lipid profile parameters and fasting blood glucose. The results obtained provide an opportunity for the assessment of risk levels, prognosis, and need for pharmacological prevention and treatment in patients with combined cardiovascular pathology. 

scholarly journals Treatment of Diabetic Condition in Streptozotocin Induced Diabetic Wister Rats Using Food Blends Such as Unripe Plantain, Soybean and Ginger

2019 ◽  
pp. 1-12
Author(s):  
I. Iwanegbe ◽  
M. Suleiman ◽  
A. Jimah
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Cell Volume ◽  
Glucose Level ◽  
Normal Control ◽  
Packed Cell Volume ◽  
Diabetic Rats ◽  
Diabetic Patients

Aims: To investigate the effect of food blends (plantain, soybean and ginger) on the blood glucose, lipid profile and haematological indices on streptozotocin induced diabetic rats. Methodology: A total of 35 rats of mean body weight 219.07 g separated into7 groups (5 per group) where induced by a single intraperitoneal (I.P) injection of streptozotocin (0.1 g dissolved in 5 ml of freshly prepared sodium citrate buffer 0.1 M, pH 4.5) at a dose of 40 mg/kg body weight after fasting for 12 hours and fed with flours/blends. The flours were produced from plant materials for different treatments/blends (blend A=100% unripe plantain, B=80% unripe plantain, 14% soybean, 6% ginger, C=70% unripe plantain, 26% soybean, 4% ginger, D= 60% unripe plantain, 38% soybean, 2% ginger, E= 50% unripe plantain, 50% soybean) and the phytochemicals and minerals content were determined. Blood glucose was determined at 5 days interval for 25 days. Diabetes was confirmed in rats with blood glucose concentrations >200 mg/dl. After 25 days rats were anaesthetized with chloroform vapour and blood samples collected by cardiac puncture for haematology and lipid profile determination. Results: The results showed that unripe plantain, soya beans and ginger in adequate proportion(C=70% unripe plantain, 26% soybean, 4% ginger or D= 60% unripe plantain, 38% soybean, 2% ginger) could help to reduce blood glucose, improve haematological parameters and lipid profile. Significant reduction was observed in the blood glucose level of rats fed blends C and D from 286 to 85 mg/dl and 307 to 90 mg/dl respectively at the end of experiment. These results also demonstrated that the inclusion of ginger at 6% causes rise in blood glucose level. Total cholesterol (TC) increased in all the blends. However, the lowest concentration of TC was observed in blends C and D. The highest packed cell volume (60%) and Haemoglobin (20 g/dl) level observed in rats fed blend C was significantly higher than the normal control fed conventional feeds. The increase in packed cell volume (PCV) (50%) and Hb (17 g/dl) in diabetic rats demonstrated that the formulated blend C was able to raise PCV and Hb above 50% and 17 g/dl (Normal control NC) respectively. Significant increase (P<0.05) in low density lipoprotein cholesterol (LDLc) was also observed in all the blends with blend C having the least (4.0 mg/dl) close to NC (2.0 mg/dl). Conclusion: From the results it is evident that blend C will manage and improve the health status of diabetic patients.

scholarly journals Effect of dietary fatty acid on blood lipid profile and induced bacterial infection in rat

2015 ◽  
Vol 1 (2) ◽  
pp. 235-243
Author(s):  
Most Sumona Akter ◽  
Md Abu Yousuf ◽  
Md Yousuf Ali ◽  
Most Mahfuja Khatun ◽  
Sukumar Saha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Lipid Profile ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Blood Cholesterol ◽  
Control Group ◽  
Blood Lipid Profile ◽  
Bacterial Colony ◽  
Group A ◽  
Group B

Ground nut is one of the commonly used decorative nutritious relish seed around the world. Ground nut have hypocholesterolamic, hypoglycemic, hypolipidemic, anti-atherosclerotic, immune-modulatory and bacterial counting effects. In Bangladesh, limited research has so far been performed on the action of ground nut in biological system and its comparative efficacy with commercialized drugs that reduce blood cholesterol. The aim of this study was carried on the effects of PUFA on blood total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride (TG) and against artificial inoculation (I/N) Staphylococcus aureus infection of experimental rat by feeding of ground nut. A total of fifteen long Evans rats (Rattus norvegicus) were used for this study. The rats were randomly divided into three equal groups (n=5) and numbered as A, B and C. Group A (control), Group B (50gm ground nut/ day/group), group C (100 gm ground nut/day/group). All groups were supplied with standard broiler pellet and fresh drinking water throughout the experimental period (January to June /2012). The blood samples were collected directly from the heart at the 1st and 60th day for biochemical test (TC, LDL, HDL and TG) and test was performed as per Memorial Diagnostic Centre, Charpara, Mymensingh. Staphylococcus aureus was cultured in nutrient broth and 100 µl of their culture was inoculated into the rats through intranasal route. Among all the treated groups, the rats of group C exhibited the lowest TC value, TG and LDL and reduced blood cholesterol significantly than (control group A) followed by group B. In bacteriological examinations, it was found that the number of bacterial colony lowest in group C in comparison with the group of A and B. From the present experiment, it can be assumed that PUFA has significant effect on blood lipid profile and against bacterial infection.Asian J. Med. Biol. Res. June 2015, 1(2): 235-243

The Effects of Soyferment-Tempeh on Lipid Profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression in Type 2 Diabetic Mice

2021 ◽  
pp. 193-200
Author(s):  
Prasetyastuti Prasetyastuti ◽  
Dian Setiawan Ghozali
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Glucose Level ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Retinol Binding Protein ◽  
Atherogenic Index ◽  
Diabetic Mice

Diabetes Type 2 can cause oxidative stress leading to the accumulation of reactive oxygen species. Soyferment-Tempeh, a fermented soybean product with aerobic and anaerobic R. oligosporus incubation has a high antioxidant content of isoflavones that can regulate oxidative stress in diabetes. In this study, we evaluate the effects of Soyferment-Tempeh on lipid profile, Retinol-Binding Protein 4 (RBP4), and Phosphoenolpyruvate Carboxykinase (PEPCK) gene expression in type 2 diabetic mice. A total of 30 mice with the age of eight weeks were divided into six groups as follows: A) nondiabetic, B) diabetic mice, C) diabetic mice with metformin, D), E), and F) diabetic mice with Soyferment-Tempeh doses of 10, 20, or 40mg/100g body weight (BW), respectively, were administered treatments orally by gavages. Blood was collected for assessment of blood glucose level, and lipid profile before and after 3 weeks of the administration. After sacrificing the mice, livers were used for RBP4 and PEPCK gene expression assessment. Supplementation with three different doses of Soyferment-Tempeh in streptozotocin-induced diabetic mice for 21 days significantly (p<0.001) reduced blood glucose level, total cholesterol, triglycerides, low-density lipoprotein level, atherogenic index, and increased high-density lipoprotein level. There was a significant decrease in RBP4 gene expression in the Soyferment-Tempeh of dose 10mg/100g BW treatment groups (p<0.05), while the PEPCK gene expression did not significantly differ (p>0.05). These results demonstrate that supplementation with Soyferment-Tempeh decreases blood glucose level, atherogenic index, improves lipid profile, and decreases RBP4.

scholarly journals Plasma lipid profile of mice fed diet supplemented with broiler fat

2014 ◽  
Vol 43 (1) ◽  
pp. 21-24 ◽  
Author(s):  
GB Das ◽  
ME Hossain ◽  
MA Akbar
Keyword(s):  
Lipid Profile ◽  
Palm Oil ◽  
Low Density Lipoprotein ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Dietary Treatment ◽  
Blood Lipid ◽  
Blood Cholesterol ◽  
Control Group ◽  
Blood Lipid Profile

This study was conducted with 180 female Swiss albino mice to examine the effects of supplementing broiler fat in the diets of mice on blood lipid profile. Twenty one days old mice were collected from ICDDRB, Mohakhali, Dhaka. They were randomly distributed in four groups having three replications each. Each replicate group had 15 mice. Results revealed that supplementation of broiler fat in mice diet significantly changed (p<0.05) triglyceride (TG) and low density lipoprotein (LDL) among groups. The control group (T0) had the highest level of cholesterol and LDL. Cholesterol, TG and LDL markedly increased (p<0.05) in all dietary treatment groups, while high density lipoprotein (HDL) significantly (p<0.01) decreased after supplementation of experimental diets. It was concluded that all the dietary treatments in general increased blood cholesterol, TG and LDL in mice fed broiler fat with different oil supplements. However, the soybean and palm oil group was comparatively better than other groups in respect of cholesterol, HDL and LDL at the blood lipid profile of mice. Therefore, it can be concluded that consumption of broiler fat with soybean and palm oil could be less harmful for mice.DOI: http://dx.doi.org/10.3329/bjas.v43i1.19380 Bang. J. Anim. Sci. 2014. 43 (1): 21-24

scholarly journals UJI EFEKTIFITAS SINBIOTIK KEFIR TEPUNG PISANG BATU TERHADAP KADAR GLUKOSA DARAH DAN KADAR PROFIL LIPID TIKUS MODEL SINDROM METABOLIK Effectiveness Test of banana (Musa balbisiana) Flour Kefir Synbiotic on Blood Glucose Level and Lipid Profile Level in Metabolic Syndrome Rats Model

2021 ◽  
Vol 16 (3) ◽  
pp. 296
Author(s):  
Taufik Maryusman ◽  
Christine Diane Dien ◽  
Santi Herlina Mail
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Glucose Level ◽  
Intervention Group ◽  
Control Group ◽  
Musa Balbisiana ◽  
Group I ◽  
Bonferroni Test

Metabolic Syndrome is a metabolic disorder characterized by hyperglycemia, hypertriglyceridemia, and dyslipidemia which can be repaired through synbiotics. This study is to analyzed the effects of banana (Musa balbisiana) flour kefir synbiotic on blood glucose level and lipid profile level of Metabolic Syndrome rats. This is true experimental study with pre-posttest with control goup design of 24 male Sprague Dawley rats which were divided into 4 groups randomly. Negative control goup (K-) given standard food only, positive control group (K +) given standard food with High Fat Fructose Diet (HFFD), intervention group I (PI) and intervention group II (PII) were given standard food with HFFD and banana (Musa balbisiana) flour kefir synbiotic 1,8 ml/200 g weight/day (PI) and 3,6 ml/200 g weight/day (PII) for three weeks. Blood glucose level was analyzed using glucose oxidase-peroxidase amino antipyrine method, cholesterol level was analyzed using cholesterol oxidase-peroxidase amino antipyrine, and triglyceride level was analyze using glycerol 3 phosphate oxidase phenol amino phenazone method. Result of Analysis of Varian test shows significant differences in mean between group (p=0,000) followed by PostHoc Bonferroni test or Post Hoc Games-Howel test which showed a mean difference in the PI and PII groups. Banana (Musa balbisiana) flour kefir synbiotic can reduce blood glucose level and improve lipid profile level.

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