scholarly journals ANTIFUNGAL ACTIVITY OF KOMBUCHA TEA AGAINST HUMAN PATHOGENIC FUNGI

2016 ◽  
Vol 9 (5) ◽  
pp. 253 ◽  
Author(s):  
Ari - Yuniarto ◽  
Kusnandar Anggadiredja ◽  
Riana Annisa Nur Aqidah
Keyword(s):  
Antifungal Activity ◽  
Infectious Diseases ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Microsporum Gypseum ◽  
Major Contributor ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Kombucha Tea ◽  
Bacteria And Fungi

ABSTRACTObjective: In general, infectious diseases still known as a major contributor to the number of morbidity and mortality in the worldwide besideother diseases. Infections are ordinarily caused by viruses, bacteria, and fungi. Several human pathogenic fungi involving Aspergillus flavus, Candidaalbicans, and Microsporum gypseum have responsible against infectious diseases. The objective of the research was to evaluate the antifungal activityof kombucha tea against human pathogenic fungi such as A. flavus, C. albicans, and M. gypseum.Methods: The antifungal activity of the kombucha tea against A. flavus, C. albicans, and M. gypseum were tested by disc diffusion method with durationof fermentation in 6, 12, and 18 days.Results: Based on the present study, inhibitory diameter of A. flavus, C. albicans, and M. gypseum are 16.83, 15.36, and 25.06 mm, respectively. Theinhibitory diameter was obtained from kombucha tea activity with duration of fermentation in 6 days.Conclusion: The results provide support for the use of kombucha tea as a potential antifungal source against human pathogenic fungi involvingA. flavus, C. albicans, and M. gypseum.Keywords: Antifungal, Kombucha tea, Pathogenic, Fungi.

The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

10.5219/1695 ◽  
2021 ◽  
Vol 15 ◽  
pp. 1112-1119
Author(s):  
Hana Ďúranová ◽  
Veronika Valková ◽  
Lucia Galovičová ◽  
Jana Štefániková ◽  
Miroslava Kačániová
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Vapor Phase ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Wheat Bread ◽  
Citrus Reticulata ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

In Vitro Evaluation of Antifungal Activity of Three Traditionally Used Medicinal Plants; Umbilicus Intermedius Boiss, Cuminum Cyminum and Zingiber Officinale Extracts

2019 ◽  
Author(s):  
Ameneh Takesh ◽  
Mahnaz Fatahinia ◽  
Ali Zarei Mahmoudabadi
Keyword(s):  
Antifungal Activity ◽  
Culture Medium ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Cuminum Cyminum ◽  
Medicinal Plant Extracts ◽  
Alternaria Sp ◽  
Medium Method

Background and Aims: The study aimed to determine the effectiveness of three medicinal plant extracts on fungi with three methods and to compare methods.  Material and methods: This study examined the antifungal properties of cumin (Cuminum cyminum L), ginger (Zingiber officinale Roscoe) and Nafe Venus (Umbilicus intermedius boiss) extracts against fungi including, Aspergillus spp., Penicillium spp., Mucor spp., Stemphylium spp., Drechslera spp., Alternaria spp., Cladosporium spp., and Aureobasidium pullulans. Furthermore, 17 candida isolates including, C. albicans, C. glabrata and C. dubliniensis were tested. In the present study two methods of disc diffusion method, agar wells diffusion method were used for assay. Then, the mixing with culture medium method was used for assessment of the antifungal activity of extracts against Alternaria sp.(as black mold), A. terreus (as hyaline mold) and C. albicans (as yeast) to compare methods as well. Results: No fungi were susceptible to extracts in disc diffusion method and agar wells diffusion method. But, this study showed that in mixing with culture medium method, cumin extract has valuable anti-fungal property and Umbilicus intermedius boiss has the inhibitory properties against the black fungi. Furthermore, it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods. Alternaria sp. and C. albicans were susceptible and resistant to all extracts.  Conclusions: it is found that mixing with culture medium method is more efficient than disc and agar well diffusion methods and inhibitory potency of the extracts varies according to the type of extraction and their concentration.

scholarly journals New Diarylheptanoid from Garuga pinnata Roxb

2013 ◽  
Vol 61 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mst Taslima Khatun ◽  
M. Mahboob Ali Siddiqi ◽  
Al-Mansur MA ◽  
MH Sohrab ◽  
AFM Mustafizur Rahman ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Microbial Growth ◽  
Stem Bark ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi

9´-Desmethylgaruganin I has been isolated from the dichloromethane extract of the stem bark of Garuga pinnata Roxb. The crude extract was screened for antimicrobial activity against a wide range of gram-positive and gram-negative bacteria and fungi by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The dichloromethane extract showed moderate inhibitory activity to microbial growth and weak cytotoxicity having LC90 25.703?g-mL–1 DOI: http://dx.doi.org/10.3329/dujs.v61i2.17058 Dhaka Univ. J. Sci. 61(2): 131-134, 2013 (July)

scholarly journals SPECIES COMPOSITION OF CANDIDA SPP. ISOLATED FROM DIFFERENT SOURCES AND THEIR SENSITIVITY TO ANTIFUNGAL PREPARATIONS

2018 ◽  
Vol 97 (3) ◽  
pp. 204-207
Author(s):  
Yana N. Frolova ◽  
M. A. Morozova ◽  
I. V. Didenko
Keyword(s):  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Microscopic Fungi ◽  
Disc Diffusion Method ◽  
Candida Spp ◽  
Adequate Treatment ◽  
Specific Composition ◽  
Different Sources

About 100,000 species of microscopic fungi were currently identified. Data on diseases caused by conditionally pathogenic fungi is particularly noteworthy. Both specific composition of Candida spp. found at fish-rearing farms of Rostov and Krasnodar regions and their susceptibility to antibiotics preparations have been studied. Strain susceptibility to drugs was determined by the disc-diffusion method. Significant variations are observed in the Candida resistance to four antibiotic preparations. Some sturgeon fingerlings and breeders have been found to be infected with candida. The drugs from the group of polyenes showed the greatest activity against the studied crops Candide. Despite the susceptibility of C. glabrata, C. guillermondii, C. tropicalis C. albicans to nystatin, the application of reabsorbing antifungals (in vivo) candidiasis of the intestine fails to be efficient. The data obtained show antibiogram to be expedient when assigning an adequate treatment of candidosis.

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

Novel N-(2-bromo-phenyl)-2-hydroxy-benzamide Derivatives with Antifugal Activity

2018 ◽  
Vol 69 (7) ◽  
pp. 1876-1880
Author(s):  
Ioana Maria Carmen Ienascu ◽  
Tiberius Balaes ◽  
Cristiana Virginia Petre ◽  
Raluca Oana Pop ◽  
Adina Cata ◽  
...  
Keyword(s):  
Pathogenic Fungi ◽  
Positive Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Chemical Methods ◽  
Yeast Saccharomyces Cerevisiae ◽  
Physico Chemical ◽  
Benzamide Derivatives ◽  
Pure Dmso

In order to increase the biological activity, some novel molecules, esters, hydrazides, hydrazones of N-(2-bromo-phenyl)-2-hydroxy-benzamide, were obtained in good yields (86-93%), working at 150 �C, 500 W, 7-11 min, under microwave irradiation. All synthesized compounds were characterized using modern physico-chemical methods (FTIR, 1H-NMR, 13C-NMR and elemental analysis). Eight dilutions in dimethyl sulfoxide of these derivatives were tested against two phyto-pathogenic fungi, Fusarium oxysporum, Sclerotinia sclerotiorum and one common yeast, Saccharomyces cerevisiae. The antifungal activity was assessed using disc diffusion method, both negative, pure DMSO, and positive control, nystatin, were used. S. cerevisiae was slightly more sensitive than filamentous fungi, the strongest inhibition, MIC=0.3125g/L, was observed for N-(2-bromo-phenyl)-2-hydroxy-benzamide and N-(2-bromo-phenyl)-2-(4-dimethylamino-benzylidene-hydrazinocarbonylmethoxy)-benzamide. The most active compounds against F. oxysporum and S. sclerotiorum were N-(2-bromo-phenyl)-2-hydroxy-benzamide (MIC= 0.625g/L), N-(2-bromo-phenyl)-2-hydrazinocarbonylmethoxy-benzamide (MIC=1.25g/L) and N-(2-bromo-phenyl)-2-(4-dimethylamino-benzylidene-hydrazinocarbonyl-methoxy)-benzamide (MIC=0.625g/L), N-(2-bromo-phenyl)-2-hydrazinocarbonyl-methoxy-benzamide (MIC=1.25g/L), respectively.

scholarly journals Antimicrobial Activity of Some Indigenous Plants of Bangladesh

1970 ◽  
Vol 7 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Mohammad S Rahman ◽  
Mohammed Z Rahman ◽  
Md Abdul Wahab ◽  
Rasheduzzaman Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Indigenous Plants ◽  
Gram Negative ◽  
Agar Disc ◽  
Bacteria And Fungi

The antimicrobial activity of methanol extracts of 17 plant species of Bangladesh was evaluated by the agar disc diffusion method. Among those, eight plant extracts exhibited potent antimicrobial activity against a wide variety of Gram positive and Gram negative bacteria and fungi at a concentration of 400 μg /disc.Key words: Antimicrobial activity, Disc diffusion method.DOI = 10.3329/dujps.v7i1.1213Dhaka Univ. J. Pharm. Sci. 7(1): 23-26, 2008 (June)

scholarly journals ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS

2017 ◽  
Vol 9 (7) ◽  
pp. 140
Author(s):  
Anto Suganya Regis ◽  
Jeya Jothi Gabriel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
High Concentration ◽  
Disc Diffusion Method ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Methanol Extracts

Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential extraction method using solvents hexane, ethyl acetate and methanol based on their polarity. The antifungal activity of the extracts was tested against 12 opportunistic fungal strains by disc diffusion method. Minimum inhibitory concentration (MIC) was determined using microtiter plate method.Results: The hexane, ethyl acetate and methanol extracts showed significant antifungal activities. The highest antifungal activity was recorded for ethyl acetate extract of C. axillaris. In disc diffusion method at high concentration (5 mg/ml), the ethyl acetate extract exhibited the zone of inhibition>30 mm against C. krusei, mentagrophytes, Scopulariopsis sp. and B. cinerea. In MIC the ethyl acetate extract inhibited the growth of T. mentagrophytes, Scopulariopsis sp., B. cinerea in its low dose (0.031 mg/ml). The hexane, ethyl acetate and methanol extracts of C. axillaris did not show activity against M. gypseum, T. rubrum and E. floccosum.Conclusion: This is the first report for the antifungal efficacy of C. axillaris. The results proved that the extracts of C. axillaris have high potential antifungal principles which could fight against the opportunistic and multidrug resistant fungal strains.

scholarly journals Antifungal Activity of TiO2/Ag Nanoparticles under Visible Light Irradiation

2020 ◽  
Vol 21 (1) ◽  
pp. 14
Author(s):  
Nahzim Rahmat ◽  
Endang Tri Wahyuni ◽  
Adhitasari Suratman
Keyword(s):  
Antifungal Activity ◽  
Visible Light ◽  
Visible Light Irradiation ◽  
Ag Nanoparticles ◽  
Irradiation Time ◽  
Light Irradiation ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Research Results

The doping of TiO2 by Ag(I) from [Ag(S2O3)2]3– contained in radiophotography wastewater by photoreduction method has been performed. TiO2/AgNPs photocatalyst was examined for its activity as an antifungal material for the inhibition of C. albicans in water under visible light irradiation. In the doping process, the weight of TiO2 was varied to obtain TiO2/AgNPs with different amounts of Ag. The TiO2/AgNPs samples were characterized by using FTIR, SRUV, TEM, SEM-EDX, and XRD methods. The antifungal test was carried out by disc diffusion method under visible light irradiation, wherein the amount of Ag-doped on TiO2, the dose of TiO2/AgNPs, and the irradiation time were optimized. The research results indicated that the antifungal activity of TiO2/AgNPs in inhibiting C. albicans has been successfully prepared. The highest inhibition was achieved by using 0.5 g/L of TiO2/AgNPs (I), at 5 h of irradiation time.

Sign in / Sign up

Export Citation Format

Share Document