scholarly journals ANTIBACTERIAL ACTIVITY OF AQUEOUS ALCOHOLIC EXTRACT OF ABUTILON INDICUM AERIAL PARTS AGAINST E .FAECALIS – AN INVITRO STUDY

2017 ◽  
Vol 10 (5) ◽  
pp. 80
Author(s):  
Jannathul Feridoz ◽  
Anitha Roy
Keyword(s):  
Antibacterial Activity ◽  
Root Canal ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Aerial Parts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Microbroth Dilution ◽  
Abutilon Indicum

Objective: To evaluate the antibacterial activity of the aqueous alcoholic extracts of Abutilon indicum aerial parts against Enterococcus faecalis.Methods: Agar well diffusion assay, as well as microbroth dilution assays, were used for determination of antibacterial activity. The assay was done intriplicate, and chlorhexidine 0.2% was used as the control.Results: Different concentrations (200, 400, and 800 mg/mL) of the ethyl acetate extract of A. indicum aerial parts showed dose-dependantantibacterial activity. The maximum zone of inhibition was 30 mm at the maximum concentration used (800 mg/mL), and the minimum inhibitoryconcentration/minimum bactericidal concentration was found to be 200 mg/mL.Conclusion: This study suggests that the aqueous alcoholic extract of A. indicum aerial parts contains promising antibacterial substances which arehaving activity against E. faecalis. E. faecalis being one of the major threats for root canal failure during endodontic treatment; plants with antibacterialactivity against E. faecalis will be promising.Keywords: Abutilon indicum, Enterococcus faecalis, Antibacterial activity, Root canal failure.

scholarly journals Phytochemical Analysis, Antibacterial Activity, FTIR and GCMS Analysis of Ceropegia juncea Roxb.

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Visveshwari M. ◽  
Subbaiyan B. ◽  
Thangapandian V.
Keyword(s):  
Antibacterial Activity ◽  
Functional Group ◽  
Methanol Extract ◽  
Biological Activities ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Major Peak ◽  
Zone Of Inhibition ◽  
Natural Health ◽  
Aerial Parts

The present study was focused on the preliminary phytochemical, antibacterial activity, FTIR and GC-MS analysis of aerial parts of C. juncea. Phytochemical analysis of three extracts showed that the presence of alkaloids, tannins and flavonoids. The methanol extract of aerial parts were found to be exhibit highest zone of inhibition against S. aureus (19.3mm), P. florescence (17.6mm) and K. pneumonia (17.0mm). FTIR analysis of the methanol extract given the major peak observed was at wavenumber 3354.08cm-1 that indicates the presence of O-H Alcohol functional group. 29 components were identified through the GCMS analysis of methanol extract. From results to confirm the fact that C. juncea possesses potential of bioactive compounds which are responsible for the biological activities that is useful for natural health.

scholarly journals Antimycotic potential of Kawayang tinik against pathogenic fungal species

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Abegail G Saducos
Keyword(s):  
Fungal Species ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Scientific Communities ◽  
Comparable Activity ◽  
Agar Well Diffusion Assay ◽  
Phytochemical Studies ◽  
Diffusion Assay ◽  
Society Today ◽  
Different Parts

The importance of discovering and obtaining new, natural and sustainable sources of potential drugs have been the focus of scientific communities due to the emergence of increasing cases of microbial resistance, one of the biggest health threats in our society today. This study aimed to determine the antimycotic potential of Bambusa blumeana (kawayang tinik) specifically its leaf, rhizome, root, inner culm and outer culm extracts using the agar well diffusion assay. Results of the study revealed that all kawayang tinik extracts produced statistically equal size zone of inhibition (ZOI) against Aspergillus niger at 1 mg/ ml concentration while the ethanolic root and leaf extracts showed larger ZOI against Penicillium chrysogenum compared to other kawayang tinik extracts. Furthermore, the results of the antifungal assay showed comparable activity of kawayang tinik extracts to Fluconazole, a pharmaceutically approved antifungal drug, at 1 mg/ml concentration. Phytochemical studies further revealed the presence of alkaloids, tannins, phenols, sterols, triterpenes and flavonoids in its different parts which may support its potential antimycotic properties.

scholarly journals Estimation of Alkaloids and Antibacterial Activity of Aconitum spicatum Bruhl Stapf from Manaslu Conservation Area

2013 ◽  
Vol 13 (1) ◽  
pp. 67-71
Author(s):  
Sajan L Shyaula ◽  
Ash B Ngakushi ◽  
Bijaya L Maharjan ◽  
Mangala D Manandhar
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Conservation Area ◽  
National Priority ◽  
Pathogenic Microbes ◽  
The Government ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

Aconitum spicatum is one of the deadly poisonous and highly valued medicinal plant. It is a national prioritized herb among 30 national priority herbs listed by the government of Nepal for their development, research and cultivation. The acute toxicity of the extract of this plant appeared to be directly related to the alkaloid content. The amount of total alkaloid of A. spicatum collected from Manaslu conservation area was calculated to be 1.7% by spectrophotometric method based on Dragendorff’s reagent. In-vitro evaluation of the crude extract of plant rhizomes using agar well diffusion assay against eight bacteria, Staphylococcus aureus, Escherichia coli, Schigella flexneri, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Salmonella typhimurium and Klebsiella pneumonia, displayed potential antibacterial activity. The diethyl ether fraction was the most effective against all pathogenic microbes with minimum bacterial concentration value 3.125-6.25 mg/ml. Nepal Journal of Science and Technology Vol. 13, No. 1 (2012) 67-71 DOI: http://dx.doi.org/10.3126/njst.v13i1.7443

scholarly journals Antibacterial evaluation of Styrax pohlii and isolated compounds

2013 ◽  
Vol 49 (4) ◽  
pp. 653-658 ◽  
Author(s):  
Camila Spereta Bertanha ◽  
Susane Hellen Utrera ◽  
Valéria Maria Melleiro Gimenez ◽  
Milton Groppo ◽  
Márcio Luis Andrade e Silva ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Preliminary Evaluation ◽  
Microdilution Method ◽  
Aerial Parts ◽  
Broth Microdilution Method ◽  
Weak Antibacterial Activity ◽  
Mic Value

The antibacterial activity of the compounds egonol (1) and homoegonol (2), of the crude ethanolic extract of Styrax pohlii (Styracaceae) aerial parts (EE), and of its n-hexane (HF), EtOAc (EF), n-BuOH (BF), and hydromethanolic (HMF) fractions was evaluated against the following microorganisms: Streptococcus pneumoniae (ATCC 6305), S. pyogenes (ATCC 19615), Haemophilus influenzae (ATCC 10211), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 10031). The broth microdilution method was used for determination of the minimum inhibitory concentration (MIC) during preliminary evaluation of antibacterial activity. The EE yielded MIC values of 400 µg/mL for S. pneumoniae and P. aeruginosa and 300 µg/mL for H. influenzae. The HF and EF fractions exhibited enhanced antibacterial activity, with MIC values of 200 µg/mL against S. pneumoniae, but only EF displayed activity against H. influenzae (MIC 200 µg/mL). The best MIC value with compounds 1 and 2 (400 µg/mL) was obtained for (1) against S. pneumoniae and P. aeruginosa. Therefore, 1 exhibited weak antibacterial activity against these standard strains.

scholarly journals Potential Antibacterial Activity of Bergenia purpurascens

2012 ◽  
Vol 12 ◽  
pp. 157-162 ◽  
Author(s):  
Gan B Bajracharya ◽  
Rokesh Maharjan ◽  
Bijaya L Maharjan
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Salmonella Typhi ◽  
Traditional Use ◽  
Conservation Area ◽  
Healing Of Wounds ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
And Staphylococcus Aureus

Bergenia purpurascens, an important traditional medicinal plant used as Ayurveda, was collected at 3800 m from the Manaslu Conservation Area. In vitro evaluation of the crude extracts of the plant rhizomes using agar well diffusion assay against eight bacteria displayed potential antibacterial activity. The minimum inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella paratyphi, Salmonella typhi, Salmonella typhimurium, Shiegella flexneri and Staphylococcus aureus were determined. This study supports the traditional use of the plant material for the healing of wounds and antibiotic effect.DOI: http://dx.doi.org/10.3126/njst.v12i0.6494 Nepal Journal of Science and Technology 12 (2011) 157-162 

scholarly journals THE DEVELOPMENT AND VALIDATION OF THE METHOD TO QUANTIFY THE AMOUNT OF PHENOLIC ACIDS IN AERIAL PARTS OF ZIZIPHORA CLINOPODIOIDES LAM.

2019 ◽  
pp. 161-168 ◽  
Author(s):  
Valeriya Yur'yevna Andreeva ◽  
Galina Il'inichna Kalinkina ◽  
Valeriya Vladimirovna Li
Keyword(s):  
Quantitative Determination ◽  
Phenolic Acids ◽  
Aerial Part ◽  
Raw Material ◽  
Alcoholic Extract ◽  
Extraction Temperature ◽  
Aerial Parts ◽  
Highly Sensitive ◽  
Development And Validation

Development and validation of method for quantitative determination of the amount of phenolic acids in the aerial part of the Ziziphora clinopodioides Lam. Objective: to develop and conduct a validation assessment of the method for determining the content of the amount of phenolic acids in the Ziziphora clinopodioides. The method of quantification of phenolic acids equivalent to chlorogenic acid by direct spectrophotometry in aerial parts of the Ziziphora clinopodioides has been developed based on the study of the absorption spectrum of an aqueous-alcoholic extract. The influence of the following factors on the yield of phenolic acids was studied: the nature of the extractant, the ratio of raw material: extractant, the degree of particle fineness, the extraction multiplicity, the extraction temperature; their optimum values were established. The method of quantitative determination of the amount of phenolic acids in the aerial part of the Ziziphora clinopodioides is validated; it has been established that the proposed method is highly sensitive, correct, reproducible and suitable for use in an analytical laboratory based on the data obtained.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Cordia verbenacea from the Venezuelan Andes

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Gina Meccia ◽  
Luis B. Rojas ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Alfredo Usubillaga ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Germacrene D ◽  
Diffusion Assay

The essential oil of Cordia verbenacea D.C. (Boraginaceae) that grows in Mérida-Venezuela was obtained by hydrodistillation from the aerial parts of the plant, yielding 0.21%. The oil was analyzed by GC-FID and GC-MS. Thirty one components which made up 94.3% of the oil were identified. The most abundant constituents found were: tricyclene (23.9%), bicyclogermacrene (11.7%), germacrene D (9.9%) and β-caryophyllene (8.2%). Antibacterial activity determination was carried out according to the disc diffusion assay. Activity against Gram-positive bacteria Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212, at a minimal inhibitory concentration (MIC) of 170 μg/mL and 200 μg/mL, was found.

scholarly journals Antibacterial Potential of the Extracts of the Leaves of Azadirachta indica Linn.

2011 ◽  
Vol 3 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Saradhajyothi KOONA ◽  
Subbarao BUDIDA
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Micrococcus Luteus ◽  
Chloroform Extract ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity ◽  
Agar Well Diffusion Assay ◽  
Negative Controls ◽  
Diffusion Assay ◽  
Antibacterial Potential

Azadirachta indica A. Juss (syn. Melia azadirachta) is well known in India and popularly known as Indian neem. To evaluate antibacterial potential, the agar well diffusion assay was used against Gram-negative and Gram-positive bacteria. Penicillin and Dimethyl sulfoxide were used as positive and negative controls, respectively. Methanol extract showed the highest and chloroform extract showed moderate to good antibacterial activity. Proteus vulgaris and Micrococcus luteus were the most susceptible bacteria while Bacillus subtilis was more resistant bacterium to the hexane, chloroform and methanol extracts of neem. The study recommended for the isolation and separation of bioactive compounds responsible for the antibacterial activity.

ANTIBACTERIAL ACTIVITY OF Zingiber officinale AND Zingiber zerumbet ESSENTIAL OILS EXTRACTED BY USING TURBO EXTRACTOR DISTILLATOR (TED)

2015 ◽  
Vol 77 (3) ◽  
Author(s):  
Noor Amirah Azelan ◽  
Rosnani Hasham ◽  
Mohd Azrie Awang ◽  
Roslinda Abd Malek ◽  
Nur Fashya Musa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Zingiber Officinale ◽  
Therapeutic Agents ◽  
Negative Control ◽  
Zone Of Inhibition ◽  
Zingiber Zerumbet ◽  
Escherichia Coli Bacteria ◽  
Diffusion Assay

Plants Zingiber officinale (ginger) and Zingiber zerumbet (lempoyang) of Zingiberaceae family have been traditionally used as treatment for stomach problems, nausea, vomiting, epilepsy, sore throat, muscular pains and several other disorders. In this study, essential oils from both plants were investigated for their efficacy on antibacterial activity against two Gram positive (Staphylococcus aureus, and Bacillus cereus) and two Gram negative (Pseudomonas aeruginosa and Escherichia coli) bacteria species using the disc diffusion assay. A zone of inhibition was compared with the standard antibiotic chloramphenicol, whilst a blank disc impregnated with the methanol was used as negative control. At concentration 20 mL/disc, Z. officinale essential oil produced zone of inhibition ranging from 16 to 36 mm, while Z. zerumbet essential oil produced zone inhibition ranging from 11 to 14 mm. These findings showed Z. officinale essential oil inhibited the growth of all tested bacteria with large zone of inhibition. The most susceptible bacteria was B. cereus while the lowest was P. aeruginosa. It can be concluded that, Z. officinale and Z. zerumbet essential oils might provide potential therapeutic agents against bacterial infection.

scholarly journals IN VITRO ANTIMYCOTIC EVALUATION AND PRELIMINARY COMBINATORIAL STUDIES OF IBUPROFEN WITH STANDARD ANTIFUNGAL DRUGS AGAINST ASPERGILLUS SPP.

2017 ◽  
Vol 10 (8) ◽  
pp. 113
Author(s):  
Mrridula Dangi Narwal ◽  
Meenakshi Balhara ◽  
Renu Chaudhary ◽  
Anil Kumar Chhillar
Keyword(s):  
Spore Germination ◽  
Inhibitory Concentration ◽  
Antifungal Drugs ◽  
Germination Inhibition ◽  
Zone Of Inhibition ◽  
Dilution Assay ◽  
Invasive Mycoses ◽  
Diffusion Assay ◽  
Microbroth Dilution

Objective: The prevalence of invasive mycoses is increased in the immunocompromised patients with an increase in resistance developed againstcurrent antifungal drugs. This has led to the need for discovering novel combinations of the antifungal drugs to combat against resistant pathogenic spp.This study mainly targets to evaluate the antifungal activity of ibuprofen (IBU) alone and in combination with the standard antifungal drugs (polyenesand azoles) against eight isolates of Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger.Methods: The study was performed using the disc diffusion assay (DDA), microbroth dilution assay and spore germination inhibition assay. Moreover,cytotoxicity was checked by heamolytic assay.Results: Minimum inhibitory concentration (MIC) of IBU against A. fumigatus and A. flavus using DDA is found to be in the range of 250-275 μg/disc while for A. niger isolates, the range was 500-575 μg/disc. Likewise, by broth microdilution assay and spore germination inhibitory assay, MICdetermined, were in the range of 500-750 μg/ml against A. fumigatus and A. flavus while for A. niger, it was 1000-1500 μg/ml.Conclusion: IBU demonstrated its antimycotic potential against all the eight isolates of Aspergillus spp. Moreover, preliminary combinatorialevaluation of IBU with the standard antifungal drugs reported by DDA revealed an increase in zone of inhibition as compared to the drugs alone.Further research regarding the confirmation of synergistic interaction between the selected drugs is in progress.

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