scholarly journals Antimycotic potential of Kawayang tinik against pathogenic fungal species

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Abegail G Saducos
Keyword(s):  
Fungal Species ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Scientific Communities ◽  
Comparable Activity ◽  
Agar Well Diffusion Assay ◽  
Phytochemical Studies ◽  
Diffusion Assay ◽  
Society Today ◽  
Different Parts

The importance of discovering and obtaining new, natural and sustainable sources of potential drugs have been the focus of scientific communities due to the emergence of increasing cases of microbial resistance, one of the biggest health threats in our society today. This study aimed to determine the antimycotic potential of Bambusa blumeana (kawayang tinik) specifically its leaf, rhizome, root, inner culm and outer culm extracts using the agar well diffusion assay. Results of the study revealed that all kawayang tinik extracts produced statistically equal size zone of inhibition (ZOI) against Aspergillus niger at 1 mg/ ml concentration while the ethanolic root and leaf extracts showed larger ZOI against Penicillium chrysogenum compared to other kawayang tinik extracts. Furthermore, the results of the antifungal assay showed comparable activity of kawayang tinik extracts to Fluconazole, a pharmaceutically approved antifungal drug, at 1 mg/ml concentration. Phytochemical studies further revealed the presence of alkaloids, tannins, phenols, sterols, triterpenes and flavonoids in its different parts which may support its potential antimycotic properties.

scholarly journals Antimicrobial activity of leaf extracts of Euphorbia paralias L. and Melilotus sulcatus Desf. against some pathogenic microorganisms

2021 ◽  
pp. 10-15
Author(s):  
Miloud M. Miloud ◽  
Najma A. Senussi
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Fungal Species ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Bioactive Agents ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The present study was aimed to investigate the antimicrobial potential of leaf extracts of Euphorbia paralias and Melilotus sulcatus against four bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella sp. and two fungal species Asperigillus niger and Aspergillus flavus. The agar well diffusion assay was used to evaluate the antimicrobial activity. The effect of these extracts was most effective against the bacterial species compared to the fungal species at a used concentration (100 mg/ml). Methanolic extracts of selected plants displayed good antimicrobial activity against all tested microorganisms species, while, no activity for aqueous extracts against tested fungal species. Methanolic extracts were the most effective plant extracts against all tested bacterial species, with MIC and MBC reached 6.2 and 12.5 mg/ml, except Klebsiella sp. which was less sensitive to M. sulcatus methanolic extract and its MIC and MBC reached 12.5 and 25 mg/ml, respectively. These plant extracts which proved to be potentially effective can be used as bioactive agents to control microorganisms caused for diseases and they can be used naturally in the human and veterinary healthcare systems.

scholarly journals ANTIBACTERIAL ACTIVITY OF AQUEOUS ALCOHOLIC EXTRACT OF ABUTILON INDICUM AERIAL PARTS AGAINST E .FAECALIS – AN INVITRO STUDY

2017 ◽  
Vol 10 (5) ◽  
pp. 80
Author(s):  
Jannathul Feridoz ◽  
Anitha Roy
Keyword(s):  
Antibacterial Activity ◽  
Root Canal ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Aerial Parts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Microbroth Dilution ◽  
Abutilon Indicum

Objective: To evaluate the antibacterial activity of the aqueous alcoholic extracts of Abutilon indicum aerial parts against Enterococcus faecalis.Methods: Agar well diffusion assay, as well as microbroth dilution assays, were used for determination of antibacterial activity. The assay was done intriplicate, and chlorhexidine 0.2% was used as the control.Results: Different concentrations (200, 400, and 800 mg/mL) of the ethyl acetate extract of A. indicum aerial parts showed dose-dependantantibacterial activity. The maximum zone of inhibition was 30 mm at the maximum concentration used (800 mg/mL), and the minimum inhibitoryconcentration/minimum bactericidal concentration was found to be 200 mg/mL.Conclusion: This study suggests that the aqueous alcoholic extract of A. indicum aerial parts contains promising antibacterial substances which arehaving activity against E. faecalis. E. faecalis being one of the major threats for root canal failure during endodontic treatment; plants with antibacterialactivity against E. faecalis will be promising.Keywords: Abutilon indicum, Enterococcus faecalis, Antibacterial activity, Root canal failure.

scholarly journals Evaluation of antibacterial potentials of Parthenium hysterophorus L. leaf extract against some pathogenic and nonpathogenic bacteria

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
M. A. Kallol ◽  
N. J. Pinky ◽  
Asaduzzaman ◽  
B. K. Das ◽  
M. Rahman
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Folk Medicine ◽  
Parthenium Hysterophorus ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Pathogenic Vibrio ◽  
Zones Of Inhibition ◽  
Agar Well Diffusion Assay

Background: The herb, P. hysterophorus L. has been used in folk medicine for many years to treat neurological disorders, inflammation, fever and malaria. This study aimed to evaluate the antibacterial activity of P. hysterophorus L. leaf extracts against some pathogenic and nonpathogenic bacterial strains. Methods: Methanol and chloroform extracts of P. hysterophorus L. leaf were applied against pathogenic Vibrio (V.) parahaemolyticus ATCC 17802, Escherichia coli 0157 ATCC 43894, Sarcina (S.) lutea IFO 3232) and nonpathogenic Bacillus (B.) subtilis IFO 3026 strains by agar well diffusion assay. Visible zones of inhibition were measured. Results: The methanol and chloroform extracts of P. hysterophorus leaf exhibited remarkable antibacterial activity against all three pathogenic (V. parahaemolyticus, E. coli 0157, S. lutea) and nonpathogenic (B. subtilis) bacterial strains. Chloroform solvent extract of P. hysterophorus leaves showed highest zone of inhibition (23 mm) against gram positive pathogenic S. lutea and methanolic leaf extract showed highest zone of inhibition (26 mm) against B. subtilis. The lowest zone of inhibition was observed for gram negative pathogenic V. parahaemolyticus in the response of methanolic (9 mm) and the chloroform (10mm) leaf extract. Conclusion: The findings of this study suggest that, leaf extracts of Parthenium hysterophorus L. could be a potential source of antibacterial agents to cope with antibiotic resistance and new drug discovery.

Antimicrobial Effect of Toothpastes on Dental Caries

2021 ◽  
Vol 9 (VII) ◽  
pp. 945-951
Author(s):  
Mahasweta S. Pandit
Keyword(s):  
Comparative Analysis ◽  
Dental Caries ◽  
Agar Medium ◽  
Antimicrobial Effect ◽  
Poor Oral Hygiene ◽  
Zone Of Inhibition ◽  
Nutrient Agar Medium ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Harmful Effects

Dental caries is an infectious disease of teeth which end up in the destruction of dental hard tissue. Poor oral hygiene is one of the major factor for accumulation of microorganisms and their harmful effects. The current study was done on antimicrobial efficacy of different brands of toothpastes against organisms isolated from patients having dental caries and comparative analysis has been made between chemical and herbal based toothpastes. Primary screening of organisms had been carried out on sterile Nutrient Agar medium and five isolates (A1, A2, A3, A4 and A5) were obtained. Antimicrobial activity of toothpastes was checked using Agar well diffusion assay. In chemical based toothpastes, Close-up showed the maximum zone of inhibition of 30mm against A1 isolate and minimum efficacy was observed with Pepsodent with zone of 7mm against A4 isolate. In herbal based toothpastes, Babool showed highest effectiveness with zone of 28mm against A3 isolate and minimum with zone of 10 mm with Dabur red and Himalaya against A1 and A3 isolate. The comparative analysis showed that chemical based toothpastes are more effective than herbal based toothpastes. Further studies on probiotic approach should be done which has been a popular method used to affect oral microbial community.

scholarly journals Antimicrobial effects of Verbascum thapsus L. leaf extracts

2020 ◽  
Vol 49 (4) ◽  
pp. 1119-1125
Author(s):  
Wajid Khan ◽  
Rizwan Ullah ◽  
Zainul Wahab ◽  
Muhammad Nazir Uddin
Keyword(s):  
Bacterial Species ◽  
Alternaria Solani ◽  
Salmonella Typhi ◽  
Fungal Species ◽  
Zone Of Inhibition ◽  
Bacillus Atrophaeus ◽  
Leaf Extracts ◽  
Verbascum Thapsus ◽  
Antifungal Potential ◽  
Antibacterial And Antifungal

Antimicrobial activity of leaves of Verbascum thapsus L. using different extractions solvents was evaluated. Extracts were prepared and tested against different bacterial (Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus atrophaeus, Salmonella typhi, Citrobacter freundii and fungal species (Alternaria solani, Candida albicans, Aspergillus fumigatus). Disc and well diffusion assays were used for screening antibacterial and antifungal potential of extracts. The ethyl acetate extract produced the maximum zone of inhibition (70% ZI) against K. pneumoniae at 3000 μg/disc. Methanol extracts formed 68% ZI against S. typhi, 54% ZI against A. tumefacian and 50% ZI against C. freundii, respectively at 3000 μg/disc respectively. Against the fungal species, the methanol extract was effective and formed maximum zone of inhibition (50% ZI) against A. solani at 3000 μg/well. Hexane extract was moderately active against bacterial species but inactive against tested fungal species. T. rubrum was resistant and none of the tested extracts affected its growth.

scholarly journals Evaluation of the anti-mycotic activity of Rosemary Oil Against Candida al-bicans

2019 ◽  
Vol 10 (2) ◽  
pp. 1228-1232
Author(s):  
Shebi S ◽  
Geetha RV ◽  
Lakshmi Thangavelu Lakshmi Thangavelu
Keyword(s):  
Antifungal Activity ◽  
Fungal Growth ◽  
Fungal Species ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Rosemary Oil ◽  
Athlete’S Foot ◽  
Systemic Infections ◽  
Sterile Filter

An antifungal medication, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycoses such as athlete's foot, ringworm, candidiasis, serious systemic infections such as Cryptococcal meningitis, and others. In traditional medicine, extracts and essential oil from flowers and leaves are used in the belief they may be useful to treat a variety of fungal disorders. The aim of this study was to analyse the antimycotic properties of rosemary oil and its principal components. The Rosemary oil was screened for antifungal activity by the disc diffusion method. Activated cultures of Candida albicans in Sabouraud’s broth was adjusted to 0.5 McFarland standards [108cfu/ml]. 100 µl of the inoculum was introduced to molten Sabourauds dextrose agar and poured in the sterile Petri plates and allowed to set. Sterile filter paper discs (6.0 mm diameter) impregnated with 25µl, 50µl and 100µl /disc were placed on fungal seeded plates and incubated at 28oC for 48 hrs. Clear zones within which fungal growth was absent were measured and recorded as the diameter (mm) of complete growth inhibition. All the concentrations of the test solution inhibited the fungal species with varying degree of sensitivity. The extract showed good antifungal activity at different concentrations with a maximum zone of inhibition of 38 mm at concentration 100µl. This study provides a sample large enough to determine the antifungal properties of Rosemary oil and suggests further studies for possible therapeutic use.

scholarly journals GC-MS Analysis and Antimicrobial Activities of Ethanol Alkaloid Leaf Extracts of Delonix elata.L

2021 ◽  
Vol 68 (2) ◽  
Author(s):  
D. Muthuselvam ◽  
Kathick Kathick
Keyword(s):  
Present Finding ◽  
Cell Formation ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Escherchia Coli ◽  
Physiological Abnormalities

Delonix elata L., belongs to family Fabaceae used by the traditional various medicinal practices to cure jaundice, skin disease, heart disease, cancer cell formation, physiological abnormalities, heptoprotective, bronchial and rheumatic problems. The present study was screen the antimicrobial and phytochemical activity of alkaloid leaf extracts. This extracts was assessed on multidrug resistant clinical isolated from both gram positive, gram negative and antifungal strains including Bacillus subtilis, Staphylococcus aureus, Escherchia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The zone of inhibition was determined by Agar well diffusion method with various concentration. GC- MS analysis was performed to identify major bioactive compounds present in the extracts. The GC – MS studies shown the present of 25 compound were identified in the leaf extract composition. The antimicrobial analysis revealed that C. albicans showed a highest zone of inhibition 25mm at 100 mg/ml of extracts. Present finding suggest that D. elata as plant pharmaceutical and pharmacological importance.

scholarly journals Synthesis, Molecular Docking and Antimicrobial Evaluation of Some Benzothiazoles

2021 ◽  
Author(s):  
Smita J. Pawar ◽  
Amol Kale ◽  
Priya Zori ◽  
Rahul Dorugade
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Property ◽  
Docking Study ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Good Antibacterial Activity ◽  
Comparable Activity ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi

Abstract Abstract The new series of 2-(substituted amino)-N-(6- substituted-1,3-benzothiazol-2yl) acetamide BTC(a-t) has been synthesized by appropriate synthetic route from substituted 2-amino benzothiazole. The synthesized compounds were screened experimentally for its antimicrobial property against gram positive, gram negative bacteria and fungi. Zone of inhibition and minimum inhibitory concentration of compounds was determined against selected bacterial and fungal strains. Compound BTC-j N-(6-methoxy-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide and compound BTC-r N-(6-nitro-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide found to have good antimicrobial potential. The compound BTC-j has shown good antibacterial activity against S. aureus at MIC of 12.5 µg/ml, B. subtilis at MIC of 6.25µg/ml, E. coli at MIC of 3.125µg/ml and P. aeruginosa at MIC of 6.25µg/ml. No statistical difference in antimicrobial activity of standard and test compounds was found indicating test compounds have comparable activity. Further docking study was carried out to check the probable interactions with the selected protein using V-life MDS 3.5 software. (DNA gyrase, PDB: 3G75). The dock score of compounds and antimicrobial activity found to be consistent.

scholarly journals Antibacterial Activities ofGinkgo bilobaL. Leaf Extracts

2011 ◽  
Vol 11 ◽  
pp. 2237-2242 ◽  
Author(s):  
S. C. Sati ◽  
Savita Joshi
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Erwinia Chrysanthemi ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Broth Dilution

The antibacterial activity of methanol, ethanol, chloroform, and hexane extracts of the leaves of Himalayan gymnospermous plantGinkgo bilobaL. was assessed against five animal and plant pathogenic strains (Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli) employing disc-diffusion and broth-dilution assays. The methanol extract showed the highest activity (zone of inhibition of 15–21 mm) followed by ethanol (14–19 mm), chloroform (15–20 mm), and hexane (14–19 mm) extracts at 250 μg/mL. A minimum inhibitory concentration (MIC) of 7.8 μg/mL was found for the methanol extract against most of the pathogens tested.

IN VITRO ANTIMICROBIAL ACTIVITY OF CYNODON DACTYLON (L.) PERS. LEAF EXTRACTS. ANTIMICROBIAL ACTIVITY BY ZONE OF INHIBITION ESTIMATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 37-41
Author(s):  
S. M Pawaskar ◽  
◽  
K. C. Sasangan
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cynodon Dactylon ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity

The present study was undertaken to evaluate in vitro antimicrobial activity of the successive leaf extracts of Cynodon dactylon in petroleum ether, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The microorganisms used in the test were – Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Bacillus subtilis, Streptococcus pyogenes, Vibrio cholerac and Enterobacter aerogenes. Two fungal strains - Candida albicans and Saccharomyces cerevisiae were also tested to evaluate the antifungal potential of the said plant extract. The reference antibiotics chloramphenicol & ampicillin (antibacterial); nystatin & clotrimazole and (antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts.The results of the study revealed that all the seven successive extracts of the leaf powder of Cynodon dactylon ( L.) Pers. exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. The nonpolar extracts i.e. petroleum ether, ethyl acetate and acetone showed the most significant antibacterial and antifungal activity against all tested organisms. The petroleum ether and ethyl acetate extracts showing maximum inhibition in the range of 8 mm - 15 mm. This was closely followed by, acetone extract, which showed the inhibition in the range of 9 mm - 13 mm.

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