scholarly journals EDEMA-REDUCING ACTIVITY OF SARGASSUM CRASSIFOLIUM Β-GLUCAN (LAMINARAN) ON EDEMA-INDUCED RATTUS NORVEGICUS

2017 ◽  
Vol 10 (7) ◽  
pp. 311 ◽  
Author(s):  
Anies Chamidah ◽  
Asep A Prihanto ◽  
Hardoko Hardoko
Keyword(s):  
Rattus Norvegicus ◽  
Brown Algae ◽  
Inflammatory Activity ◽  
Acid Extract ◽  
Inflammatory Agent ◽  
Reducing Activity ◽  
Anti Inflammatory ◽  
Leg Edema

Objectives: The aim of this study is to investigate anti-inflammatory activity of β-glucan (laminaran) of brown algae, Sargassum crassifolium which was extracted by using two different methods, β-glucan laminaran acid extract (LAE) and laminaran modified extract (LME).Methods: β-glucan (laminaran) of brown algae, S. crassifolium have been extracted using two different methods, namely, β-glucan LAE and LME. The edema reducing activity of the extracts was investigated with carrageenan- induced leg edema male of Rattus norvegicus as a model.Results: The swelling of edema was strongly inhibited by the treatment of LME with a percentage of 81.62% after 3rd hr. It also found that the size of edema were inhibited by all treatments with the value ranging from 25.49% to 81.68%. Smallest edema size was found on LME treatment, with a size of 0.68±0.09 mm where the control was about 0.92 mm. Furthermore, all β-glucan-treated mice showed lower edema size compared to control.Conclusion: It was obvious that all type of laminaran had anti-inflammatory activity. LME is the best method for production of β-glucan (laminaran) with high anti-inflammatory activity. Hence, in this study, we reported a new anti-inflammatory agent derived from S. crassifolium.

scholarly journals SYNTHESIS NEW POTENTIAL ANTI-INFLAMMATORY AGENT SODIUM SALT OF PENTAGAMAVUNON-0

2010 ◽  
Vol 4 (3) ◽  
pp. 180-185
Author(s):  
Enade Perdana Istyastono ◽  
Rr. Sri Untari Siwi S.M.P ◽  
Andreas Asdi Utama ◽  
Supardjan A.M
Keyword(s):  
Inflammatory Activity ◽  
Physiological Changes ◽  
Solubility In Water ◽  
Curcumin Analogues ◽  
Inflammatory Agent ◽  
Curcumin Derivatives ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Living Tissues ◽  
Anti Inflammation

Inflammation is the response of living tissues to injury. The process affects physiological changes such as erythema, edema, asthma and fever. Non-steroid Anti-inflammatory Drugs (NSAIDs) have been developed since they could inhibit inflammation process because of its ability to inhibit biosynthesis of prostaglandin, one of inflammation mediators, through inhibition of cyclooxigenase (COX) enzymes. Molecules, which have been reported having anti-inflammatory activity, for example, are curcumin, some curcumin derivatives and curcumin analogues. One of curcumin analogues that has been  developed is pentagamavunon-0 (PGV-0) whose IUPAC name is 2,5-bis(4'-hidroxy-3'-methoxy-benzylidene)cyclo-pentanone. But PGV-0, which is like curcumin, practically insoluble in water, so it causes problems in the development. The aim of this research is to synthesize a derivative of PGV-0, a natrium salt of PGV-0 (natrium pentagamavunonate-0/Na-pentagamavunonate-0), which is hoped to have a better anti-inflammatory activity and solubility in water than PGV-0. PGV-0 was synthesized by reacting vanillin and cyclopentanone catalized by acid. Na-pentagamavunonate-0 was synthesized with PGV-0 as a starting material using an appropriate method. This research was able to synthesize new compound that was estimated as a natrium salt of PGV-0 (natrium pentagamavunonate-0/Na-pentagamavunonate-0).   Keywords: Curcumin, PGV-0, Na-pentagamavunonate-0, anti-inflammation

Anti-Inflammatory Activity of Ethanol Extract of Marine Sponge Petrosia Sp. by Supression the Level of Tumor Necrosis Factor-alpha

2021 ◽  
pp. 4435-4439
Author(s):  
Adryan Fristiohady ◽  
Muhammad Hajrul Malaka ◽  
Andi Rizqa Wahyuni Safitri ◽  
Dewo Diha ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Oral Treatment ◽  
Ethanol Extract ◽  
The Body ◽  
Inflammatory Activity ◽  
Inflammatory Agent ◽  
Edema Volume ◽  
Group I ◽  
Tnf Α ◽  
Anti Inflammatory

Inflammation is the host's protective response to any stimulus that harms the body. Excessive inflammatory process causes tissue damage. Therefore, an anti-inflammatory agent is needed. The use of natural ingredients, especially sea sponges, is an option to reduce the side effects of anti-inflammatory agents. This utilization is related to the discovery of new agents. So, we tested the effect of the ethanol extract of Petrosia sp. as an anti-inflammatory agent. Animal induced with 1% carrageenan and left for 1 hour. After that the animals were divided into 6 groups (n = 4) and given oral treatment, namely: Group I (normal group); Group II (negative group); Group III (ethanol extract of Petrosia sp. Concentration of 0.05mg/ml); Group IV (ethanol extract of Petrosia sp. Concentration 0.1mg/ml); Group V (ethanol extract of Petrosia sp. Concentration 0.2mg/ml); and Group VI (positive group, Diclofenac Sodium). After 1 hour, the animals were measured for edema volume and plasma TNF-α levels. Based on the research conducted, the ethanol extract of Petrosia sp. decreased edema volume and plasma TNF-α levels in inflammatory mice. The concentration of 0.2mg/mL had a significant effect on the negative control used (p <0.05). On the other hand, Petrosia sp. indicates the presence of alkaloids, flavonoids, and steroids. They may play an important role in the anti-inflammatory process. Thus, it can be concluded that the ethanol extract of Petrosia sp. has anti-inflammatory activity.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

2019 ◽  
pp. 278-281
Author(s):  
RAJESH A ◽  
DOSS A ◽  
TRESINA PS ◽  
MOHAN VR
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Potential Source ◽  
Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

scholarly journals Piper cubebaL. Methanol Extract Has Anti-Inflammatory Activity Targeting Src/Syk via NF-κB Inhibition

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Nurinanda Prisky Qomaladewi ◽  
Nur Aziz ◽  
Mi-Yeon Kim ◽  
Jae Youl Cho
Keyword(s):  
Inflammatory Activity ◽  
No Production ◽  
Immune Functions ◽  
Rt Pcr ◽  
Adaptive Immune ◽  
Inflammatory Agent ◽  
Inflammatory Mechanism ◽  
Gene Reporter Assay ◽  
Anti Inflammatory ◽  
Luciferase Gene Reporter Assay

Piper cubebaL. is a plant in the Piperaceae family that is generally found in tropical countries and acts as an antioxidant and anti-inflammatory agent. Unfortunately, the molecular mechanism of the anti-inflammatory activity has not been fully investigated. In this study, we elucidated the anti-inflammatory mechanism by focusing on NF-κB signaling, which is considered a prototypical inflammatory signaling pathway in both innate and adaptive immune functions. Cellular activity and the molecular target of Pc-ME were identified in macrophage RAW264.7 cells and HEK293T cells by assessing NO production, cytokine expression by RT-PCR, luciferase gene reporter assay, and protein regulation in cytoplasm by Western blot upon NF-κB activation. Pc-ME reduced NO production without any cell toxicity; inhibited expression of proinflammatory cytokines such as iNOS and IL-6; downregulated NF-κB activation mediated by both MyD88 and TRIF; and diminished the phosphorylation of IκBα, IKKα/β, Akt, p85, Src, and Syk. Pc-ME inhibited Syk and Src autophosphorylation during overexpression in HEK cells, which confirmed our hypothesis that Syk and Src were signaling targets of Pc-ME. These findings indicate thatPiper cubebaL. has anti-inflammatory activity by targeting Src/Syk in the NF-κB pathway.

scholarly journals Evaluation of marine brown algae Sargassum ilicifolium extract for analgesic and anti-inflammatory activity

2013 ◽  
Vol 5 (3) ◽  
pp. 146 ◽  
Author(s):  
ChandrarajC Simpi ◽  
ChannabasappaV Nagathan ◽  
SantoshR Karajgi ◽  
NavanathV Kalyane
Keyword(s):  
Brown Algae ◽  
Inflammatory Activity ◽  
Marine Brown Algae ◽  
Anti Inflammatory

scholarly journals IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ALCHORNEA CORDIFOLIA (SCHUMACH AND THONN.) MULL. ARG. AND ANTROCARYON KLAINEANUM PIERRE EXTRACTS

2020 ◽  
pp. 129-135
Author(s):  
ROSETTE CHRISTELLE NDJIB ◽  
STEVE VALDI DJOVA ◽  
CHRISTELLE WAYOUE KOM ◽  
AGBOR GABRIEL AGBOR ◽  
AMINA MAMAT ◽  
...  
Keyword(s):  
Concentration Level ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Reducing Activity ◽  
Anti Inflammatory ◽  
Β Carotene

Objective: This study aims to evaluate the in vitro antioxidant, anti-inflammatory activities of the aqueous and hydroethanolic extracts recipe of Alchornea cordifolia and Antrocaryon klaineanum. A preliminary phytochemical screening was carried out. Methods: The total phenols content was determined by the Folin Ciocalteu reagent method, while the antioxidant activity of both extracts was characterized by the 2-2diphenyl-1-picrilhidrazil (DPPH) and β-carotene assays. The anti-inflammatory activity of the extracts was evaluated as the inhibition of Bovine Serum Albumin (BSA) denaturation and antiproteinase activity. Results: The aqueous extracts of Alchornea cordifolia and Antrocaryon klaineanum contained more polyphenols [270 mg Ascorbic acid equivalent (AAE)/g dry weight (dw)] than the hydroethanolic recipe extract (262.41 mg AAE/g dw) at the same concentration level. On the other hand, the aqueous and hydroethanolic recipe extract had the same radical scavenging activity with the antiradical power of 0.851 and 0.830, respectively. Similarly, the recipe extract had the same reducing activity with reducing the power of 94.2±2.03 mg EAA/g dw and 97.4±4.16 mg EAA/g dw for the aqueous and hydroethanolic recipe extract of Alchornea cordifolia and Antrocaryon klaineanum respectively. For the anti-inflammatory activity it was observed that both extracts possess the same activity as Diclofenac® with an IC50 of 50.21 μg/ml. The phytochemical screening of the extracts revealed the presence of alkaloids, flavonoids, carbohydrates, phenols and tannins, which may account for their activities. Conclusion: The plant recipe extract studied possess antioxidant and anti-inflammatory potentials, which may be beneficiary to its consumers.

Silica catalyzed one pot synthesis of hybrid thiazolidin-4-one derivatives as anti-tubercular and anti-inflammatory agent by attenuating COX-2 pathway

2019 ◽  
Vol 49 (20) ◽  
pp. 2725-2759 ◽  
Author(s):  
Prateek Pathak ◽  
Parjanya Kumar Shukla ◽  
Vladislav Naumovich ◽  
Maria Grishina ◽  
Vladimir Potemkin ◽  
...  
Keyword(s):  
Wistar Rat ◽  
Catalytic Synthesis ◽  
Inflammatory Activity ◽  
Inhibition Assay ◽  
One Pot ◽  
Inflammatory Agent ◽  
Hybrid Class ◽  
Anti Inflammatory

A novel series of a hybrid class of hybrid thiazolidin-4-one derivatives were designed and synthesized through one-pot catalytic synthesis. The reaction was catalyzed in the presence of silica-H2SO4(+6). The derivatives computational ADMET profile was calculated. The study shows that most active derivatives have optimal logP, higher anti-inflammatory activity score, and poor metabolism at the sight of P450-3A4 and 2D6. The entire series of derivatives were further evaluated for anti-tubercular (against Mycobacterium tuberculosis H37Rv (Resistant strain)) and anti-inflammatory activity (in-vivo assay using Wistar rat). The result showed that derivatives 4c, 4h, and 4m have significant potency against tested M. tuberculosis. However, derivatives 4i and 4j found significantly active against inflammation. In vitro COX inhibition assay also supported the result in favor of selectivity and efficacy of derivatives.

scholarly journals The Analgesic and Anti-Inflammatory Activity of Linum usitatissimum in Balb/c Mice

2017 ◽  
Vol 22 (4) ◽  
pp. 892-896 ◽  
Author(s):  
Mahmoud Rafieian-kopaei ◽  
Abdollah Shakiba ◽  
Mehrnoosh Sedighi ◽  
Mahmoud Bahmani
Keyword(s):  
Antioxidant Activity ◽  
Analgesic Activity ◽  
Linum Usitatissimum ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Inflammatory Agent ◽  
Linum Usitatissimum L ◽  
Anti Inflammatory ◽  
Analgesic Activities

Linum usitatissimum L is traditionally used for relief of pain and inflammation. In this study, the analgesic and anti-inflammatory effects of this plant were evaluated. Xylene test was used for anti-inflammatory evaluation in which 48 mice were randomly designated into 6 groups of 8 each including: control, dexamethasone as positive control (15 mg/kg), and experimental groups (42, 85, 170, and 340 mg/kg, respectively). For analgesic evaluation, 192 mice were randomly designated into 4 sets of 6 groups of 8 mice, including control, morphine as positive control, morphine plus naloxone, experimental groups (200 and 500 mg/kg extract), and extract along with naloxone group, which received 500 mg/kg. The analgesic activities were evaluated at 5, 15, 30, and 60 minutes, respectively, in each set. Both doses showed analgesic activity, the 200 mg/kg possessed higher effects ( P < .05). Naloxone reduced a section of its effect ( P < .001). The 170 mg/kg dose of the extract showed anti-inflammatory activity ( P < .05). The extract had phenolic, flavonoid, and flavonol compounds with antioxidant activity. Linum usitatissimum L dose dependently had analgesic activity partially like morphine and might be used as analgesic and anti-inflammatory agent.

Anti-inflammatory activity of some water-soluble bioflavonoids

1959 ◽  
Vol 196 (6) ◽  
pp. 1205-1210 ◽  
Author(s):  
Valy Menkin
Keyword(s):  
Vitamin C ◽  
Capillary Permeability ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Inflammatory Exudate ◽  
Inflammatory Agent ◽  
Anti Inflammatory ◽  
Permeability Increase ◽  
Ph Range

The water-soluble citrus bioflavonoid mixture contained in the preparation C.V.P., without added vitamin C, is capable of inhibiting the increased capillary permeability referable in turn to an inflammatory exudate, irrespective of the pH of that exudate. The suppressing effect of these water-soluble bioflavonoids can be demonstrated in concentrations of 5 or 10 mg/0.25 ml of exudative material. They likewise inhibit the effect of leukotaxine that has been extracted from alkaline exudates. In this way they repress not only increased permeability of capillaries, but also the local emigration of leucocytes. Finally this water-soluble bioflavonoid mixture appears to behave as an anti-inflammatory agent over a larger pH range of exudates than either cortisone or corticotropin, (ACTH). Cortisone represses permeability increase and leucocytic migration of alkaline exudate. ACTH suppresses the activity of exudin in its ability to increase local capillary permeability induced by acid exudates. This bioflavonoid mixture is capable of inhibiting in this regard both alkaline and acid exudates. The possible potentials of C.V.P. are pointed out.

Sign in / Sign up

Export Citation Format

Share Document