scholarly journals DETECTION OF URER AND UREC AMONG PROTEUS MIRABILIS

2017 ◽  
Vol 10 (8) ◽  
pp. 386 ◽  
Author(s):  
Abdul Kareem Muneeralatrash ◽  
Ahlam Kadhum Al-yasseen
Keyword(s):  
Molecular Weight ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Proteus Mirabilis ◽  
Biochemical Test ◽  
Urine Samples ◽  
Molecular Technique ◽  
Bacterial Isolates ◽  
Conventional Methods

Objective: This study aimed to investigate the correlation between ureR and ureC genes with the production of urease by Proteus mirabilis.Methods: A total of 450 mid-stream urine samples have been collected from patients with urinary tract infection whom admitted to the hospitals in Annajaf Al-Ashraf province for consultancy during the period from October 2015 to February 2016.Out of 150 bacterial isolates, only 29 isolates were belong to P. mirabilis according to conventional methods (depending on microscopic and culturing examination as well as biochemical test) and molecular technique using 16SrRNA gene.Results: The results of phenotypic and genotypic detection of urease in P. mirabilis showed that all isolates were able to produce urease and possess ureR and ureC that encodes to urease by appearing of amplicon with molecular weight 359 and 533 bp, respectively, when electrophoresed on 1% agarose gel.Conclusion: A correlation has been found between ureR and ureC genes with the production of urease by P. mirabilis.

scholarly journals Isolation & Pathogenic Study on Proteus mirabilis

2010 ◽  
Vol 7 (1) ◽  
pp. 317-426
Author(s):  
Baghdad Science Journal
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Sodium Chloride ◽  
Tract Infection ◽  
Proteus Mirabilis ◽  
Bacterial Isolates ◽  
Extract Agar ◽  
Bacterial Cultures ◽  
Biochemical Studies ◽  
Deficient Medium

During the period October 2003 till July 2004, about (253) urine samples have been collected from urinary tract infection. The study has shown that the bacterium Proteus mirabilis is the responsible for (11.85%) of the urinary tracts infections. Also, the study has declared that the ratio of separation this bacterium from women was (7.51%) and it is higher than the ratio of separation in both men and children which ranged (1. 58%) and (2.76%) respectively . About (30) samples of stool have been collected from children and the ratio of isolation this bacterium has been showen to be( 30%) from children aged bellow 3 years,as well as, we have got bacterial cultures related to P.mirabilis isolated from the infections of middle-ear and bacteremia . Morphological and biochemical studies have been applied to characterize the isolation bacterium as well as other kinds of micro-orgarisms that were isolated from infections of urinary tracts in this study. The results of the study demonstrated the bacterial isolates have shown an absolute resistance with a ratio of (100%) for both the antibiotic Ceftazidime and Cephalothin. Also, the study has shown that the antibiotic Ciprofloxacin is the most effective antibiotic against this type of bacterium . The percentage of sensitivity for the local isolates to this kind of antibiotic was (96.7%) then Gentamycin and Cephotaxim and the ratio of sensitive isolates to these antibiotics were (80%) and (76.7%) respectively. We have studied some virulence factors which the bacterium owns like the production of enzymes ?-Lactamase and Extended spectrum ?–Lactamase . The study has shown that the local isolates of this bacterium produce these enzymes with a ratio of (100 %) . The study confirmed the efficiency of fish extract agar prepared locally in growing bacterium . Also , it affirmed that the fish extract agar supplemented with (4-6 %) of sodium chloride is mimcs the appearing in C.L.E.D (cystien lactose electrolyte deficient medium

Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

2018 ◽  
pp. 26
Author(s):  
Rana M. Abdullah Al-Shwaikh ◽  
Abbas Falih Alornaaouti
Keyword(s):  
Molecular Weight ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Otitis Media ◽  
Urinary Tract Infections ◽  
Gel Electrophoresis ◽  
Wound Infections ◽  
Tract Infections

       Current study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014        The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 18 isolates (78.26%) from burn followed by 8 isolate (80%) from wound infection and 5 isolates (62.5%) from urinary tract infection , finally 6 isolates (100%) from blood have this gene.

scholarly journals Using Lactobacillus as Probiotic to Inhibit Growth and Adhesion of Proteus mirabilis Causing Urinary Tract Infection

2008 ◽  
Vol 2 (1) ◽  
pp. 19-32
Author(s):  
Ghydaa H. A al-jeboury ◽  
Abdul Wahed Baker
Keyword(s):  
Urinary Tract Infection ◽  
Lactic Acid ◽  
Urinary Tract ◽  
Epithelial Cell ◽  
Tract Infection ◽  
Proteus Mirabilis ◽  
Inhibitory Effect ◽  
Adhesion Property ◽  
Significant Inhibitory Effect ◽  
Urine Specimens

The aim of the study was to use lactic acid bacteria (LAB), as probiotic, to treat growth and adhesion property of Proteus mirabilis isolated from patients suffering from urinary tract infection (UTI). For this purpose, one P. mirabilis isolate (P.M.9) was selected out of 9 isolates obtained from 150 urine specimens. Due to its resistance to 11 antibiotics tested, this isolate was treated with three-fold concentrated filtrates of two lactobacillus isolates (as probiotic). Results after treatment, showed that the filtrates exhibited significant inhibitory effect against the pathogenic P.M.9 and its adhesion property especially when only an average of 3-10 bacteria /cell were adhered to each epithelial cell compared to 44-55 bacteria/cell.

scholarly journals Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

2015 ◽  
Vol 2 (4) ◽  
pp. 31990-31990 ◽  
Author(s):  
Fariha Akhter Chowdhury ◽  
Mohammad Nurul Islam ◽  
Anamika Saha ◽  
Sabrina Mahboob ◽  
Abu Syed Md. Mosaddek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples

scholarly journals Multidrug Resistance of Uropathogens at Governmental Hospitals in the Gaza Strip/Palestine

10.3823/855 ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sameh A. Alkhodari ◽  
Abdelraouf A. Elmanama
Keyword(s):  
Urinary Tract Infection ◽  
Multidrug Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Gaza Strip ◽  
Public Health Problem ◽  
Urine Samples ◽  
E Coli ◽  
The Gaza Strip ◽  
Klebsiella Spp

Urinary tract infection is a public health problem worldwide. E. coli and klebsiella are among the main etiologic for UTI in Gaza Strip. The growing variations in resistance among uropathogens to antimicrobials is multifactorial and varies globally. It greatly reduces/limits or complicate treatment option. Aims: To determine the pattern of antimicrobial resistance and multidrug resistance among uropathogens at governmental hospitals. Methods: We analyzed the data of 11,890 urine samples processed in governmental hospitals in the Gaza Strip, Palestine during 2019. The percentage of resistance was calculated for uropathogens, and then multidrug resistance was calculated according to “CDC” definition. Results: Of 11,890 urine samples, 2910 (24.5%) showed significant growth.  Escherichia coli was isolated most frequently (1743; 59.9%), followed by Klebsiella spp. (725; 24.9%), Pseudomonas spp. (123; 4.2%), Streptococcus spp. (98; 3.4%), Staphylococcus aureus (41; 1.4%). Microorganisms resistance was high against Ampicillin (92.4%) and Amoxicillin (91.1%), Co-Trimoxazole (68.2%), Cefalexin (64.9%), Doxycycline (61.9%), Nalidixic acid (53.6%), Cefuroxime (53.0%), Ceftriaxone (48.9%), Ceftazidime (43.1%), Ciprofloxacin (36.9%), Gentamicin (25.8%), Amikacin (3.2%). The resistance of microorganisms in males is higher than females. Multidrug resistance was detected in 37% of E. coli and 53% in Klebsiella spp. Conclusion: Resistance is high and variable among uropathogens isolated from patients in Gaza strip. Both age and gender are risk factors in both infection and resistance pattern. The multidrug resistance percentage is growing remarkably in Gaza Strip. Keywords: Uropathogens, Resistance, Urinary tract infection, Multidrug resistance, Gaza strip, Palestine  

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