scholarly journals INVESTIGATION ON IN VIVO ANALGESIC ACTIVITY OF METHANOL EXTRACT OF MARINE BROWN ALGA SPATOGLOSSUM ASPERUM J. AGARTH

2018 ◽  
Vol 11 (11) ◽  
pp. 313
Author(s):  
Pandithurai M ◽  
Murugesan S ◽  
Sivamurugan V
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Brown Alga ◽  
Methanol Extract ◽  
Albino Rats ◽  
Significant Activity ◽  
Study Evaluation ◽  
Wistar Albino Rats

Objective: In the present study, evaluation of the methanol extract of Spatoglossum asperum, brown marine algae on the analgesic activity using animal model was focussed.Methods: Acetic acid-induced writhing test on Wistar albino rats at a dosage of 10 mg/kg body weight of algal extract have been carried out. It showed significant analgesic activity by reducing the number of acetic acid-induced writhing.Results: The animals at a dosage of 10 mg/kg body weight exhibited, 70.52% of the animals were protected using S. asperum extract, on the other hand, the standard, diclofenac protected 84.21% of the animals. The results are statistically significant at p<0.001, and the investigation revealed dose-dependent significant activity in comparison with standard and control.Conclusion: Hence, it can be concluded that the methanol extracts of the brown alga S. asperum have potent analgesic activity at moderate doses.

scholarly journals Analgesic and Antidiarrheal Activities of Leaf of Podocarpus neriifolius D. Don

2016 ◽  
Vol 19 (2) ◽  
pp. 215-218
Author(s):  
Md Rahatullah Razan ◽  
Muhammed Mahfuzur Rahman ◽  
Faiza Tahia ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Castor Oil ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Activity Assay ◽  
Swiss Albino Mice

The methanol extract of leaf of Podocarpus neriifolius D. Don exhibited in vivo peripheral analgesic and antidiarrheal activities in Swiss Albino mice. In the peripheral analgesic activity assay, the methanolic extract showed 50.00 ± 8.57% and 70.25 ± 1.18% inhibition of acetic acid-induced writhing at 200 and 400 mg/kg body weight, respectively. In addition, the extract also revealed a dose dependant inhibition of castor oil- induced diarrhea with 43.77 ± 3.13% and 56.23 ± 6.49% inhibition of feces at 200 and 400 mg/kg body weight, respectivelyBangladesh Pharmaceutical Journal 19(2): 215-218, 2016

scholarly journals “EVALUATION OF GALPHIMIA GLAUCA STEM METHANOL EXTRACT FRACTIONS FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES”

2019 ◽  
pp. 266-272
Author(s):  
GARIGE BABA SHANKAR RAO ◽  
SRISAILAM K ◽  
V UMA MAHESHWARA RAO ◽  
VASUDHA B
Keyword(s):  
Acetic Acid ◽  
Methanol Extract ◽  
Dose Range ◽  
Experimental Studies ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Methanol Fraction ◽  
Cotton Pellet ◽  
Anti Inflammatory

Objective: This current investigation assesses in vivo central and peripheral analgesic effects and anti-inflammatory properties of fractions obtained from Galphimia glauca (GG) stem methanol extract. Methods: The laboratory models such as Swiss albino mice and Wistar albino rats were employed in the studies. The GG stem methanol extract was subjected to fractionation with solvents such as hexane, chloroform, ethyl acetate, and methanol. Orally, the dose range of 100, 200, and 400 mg/kg was given for 1 day for evaluating analgesic (hotplate test, tail clip test, writhing test, and formalin test) and weekdays for assessing anti-inflammatory activity (carrageenan and cotton pellet test methods), respectively. The experimental studies were further conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by prechallenging it with naloxone and acetic acid, respectively. The in vivo anti-inflammatory studies were conducted using carrageenan-induced rat paw edema model and cotton pellet granuloma test. Results: The LD50 of the extract was found to be >2000 mg/kg b.w. The methanol fraction of 400 mg/kg dose exhibited significant (p≤0.001) and dose-dependent analgesic and anti-inflammatory activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid, respectively. Conclusion: The results revealed that the stem methanol fraction has more potential in terms of analgesic and anti-inflammatory properties.

scholarly journals In vivo Analgesic activity of methanolic extract of Helianthus annuus seeds

2016 ◽  
Vol 5 (4) ◽  
pp. 38-40 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Ahmed Tanjimul Islam ◽  
Mir Monir Hossain ◽  
Kishor Mazumder
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Helianthus Annuus ◽  
Analgesic Activity ◽  
Latency Period ◽  
Pharmaceutical Journal ◽  
Mice Model ◽  
Hot Plate ◽  
Writhing Test

The sunflower seed is the seed of the sunflower (Helianthus annuus). The methanol extract of seeds of Helianthus annuus were screened for analgesic activity in mice model to systematically explore the medicinal values of the plant. Acetic acid induced writhing and hot plate methods were used to confirm the central and peripheral analgesic action. In case of acetic acid-induced writhing test the extract showed significant (P <0.05) analgesic potential at doses 100 and 200 mg/kg body weight (50.35 and 57.85% inhibition, respectively). In the hot plate method, increase (p < 0.05) of latency period was also observed in comparison to standard aspirin. At 60 minutes, the latency period of two different doses (100 and 200 mg/kg body weight) was found at 13 ± 0.91 and 16.5 ± 1.55 second. The results obtained support the use of Helianthus annuus seeds in painful conditions acting both centrally and peripherally.Islam et al., International Current Pharmaceutical Journal, March 2016, 5(4): 38-40http://www.icpjonline.com/documents/Vol5Issue4/02.pdf

Chemical composition analysis and in vivoAnti-diabetic activity of aqueous extract of aerial part of Pallenis spinosa in diabetic rats

2021 ◽  
Vol 17 ◽  
Author(s):  
Abdelkrim Khettaf ◽  
Seloua Dridi
Keyword(s):  
Body Weight ◽  
Chemical Analysis ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Control Group ◽  
Albino Rats ◽  
Spectrometric Analysis ◽  
Composition Analysis ◽  
Significant Activity

Background: The aim of this work is to confirm the anti-diabetic activity of Pallenis spinosa as recommended in traditional medicine and the chemical analysis of the aerial part of Pallenis spinosa. Methods: The isolated compounds have been identified by the chemical methods and spectrometric analysis such as: UV, 1H NMR, and 13C NMR spectroscopy. With regard to anti-diabetic activity, a series of experiments have been carried out in vivo on Westar albino rats. Diabetes has been induced in the animals by an intraperitoneal injection of streptozotocine; they have been treated a day with an aqueous extract from the aerial part infusion (250 and 500 mg/kg body weight) and glibenclamide (5 mg/kg body weight) for 21 days. Results: The chemical analysis of the aerial part of Pallenis spinosa has led to the isolation of five known flavanoid: Patuletin 7-galactopyranoside, patuletin-3-O-α-L-rharnnopyranosyl (1-6)-β-D-galactopyranoside, tricin 7-glucopyranoside, tricin and quercetin. The aqueous extract of both doses 250 mg and 500 mg has shown a significant activity in reducing blood sugar with 43.38% for the dose of 250 mg / kg and 37.76% for the dose of 500 mg / kg, as well as a significant decrease in the total fatness, triglycerides, and the total cholesterol levels in animals Treatment compared to diabetes control group (P = 0.05). We’ve used Glibenclamide as a reference, and it has shown similar results. Conclusion: In order to explore and develop new anti-diabetes drugs, more studies are needed on this plant for scrutinizing its mechanism of activity.

scholarly journals Onosma bracteatum wall: A Potent analgesic agent

2018 ◽  
Vol 17 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Hina Imran ◽  
Atiq Ur Rahman ◽  
Tehmina Sohail ◽  
S Intasar H Taqvi ◽  
Zahra Yaqeen
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Latency Period ◽  
Maximum Effect ◽  
Tail Flick ◽  
Significant Activity ◽  
Tail Flick Test ◽  
Writhing Test ◽  
Post Feeding

Background: This study was aimed to find out the central and peripheral analgesic activity of hydro methanolic extract of aerial parts of Onosma bracteatum.Material and methods: The central and peripheral analgesic activity is evaluated by tail flick test and acetic acid induced writhing test at the doses of 50, 100, 250 and 500mg/kg body weight respectively in animal models.Results: The results obtained from Tail flick test revealed that O. bracteatum possesses potent analgesic effects by inducing significant increase in latency period in dose dependent manner at all doses at 1, 2 and 3 hours post feeding respectively. The maximum effect was observed at a dose of 500mg/kg i.e. 258.9% (p<0.05) at 3hrs post feeding. Diclofenac sodium (5mg/kg body weight) run as standard also increased the latency period continuously and highest activity was noted at 3hr i.e. 284.5% (p<0.05). Acetic acid induced writhing test also showed significant activity in a similar manner by O. bracteatum i.e 54% (p<0.05) at 500mg/kg while standard drug Diclofenic sodium (5mg/kg body weight) showed 45.9% (p<0.05) activity.Conclusion: It is concluded that O. bracteatum possesses significant central and peripheral analgesic activity in animal model.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.36-41

ANTIDIABETIC EVALUATION OF CHONEMORPHA MACROPHYLLA ROOTS BY IN VIVO METHODS USING MALE WISTAR ALBINO RATS AND ITS MECHANISM OF ACTION

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (12) ◽  
pp. 42-51
Author(s):  
K. R. Krishna Kumar ◽  
◽  
K. K. Srinivasan
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glucose Uptake ◽  
Normal Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Wistar Albino Rats ◽  
Isolated Rat

Antidiabetic activity of 95% and 50% ethanol extracts of C. macrophylla roots was evaluated using male Wistar albino rats to gather support for the promising results of glucose uptake against normal control in studies conducted on L-6 muscle cell lines as well as isolated rat hemidiaphragm. Initially, preliminary phytochemical studies were performed and identified the presence of phenolics, flavonoids and their glycosides. Before doing in vivo experiment, by following RRR concept, glucose uptake in L-6 muscle cell lines were performed, detected better glucose uptake against normal control and decided to do in vivo experiments. The estimation of glucose uptake in isolated rat hemidiaphragm treated with the extract was employed for the study of peripheral glucose uptake and the results were significant. Content of blood glucose was high in STZ-diabetic rats as compared to normal rats. Treatment of STZ-diabetic rats with the test extract ME -CM RH significantly reduced the hyperglycaemia when compared with STZ only treated rats. Rats lost their body weight after STZ treatment, which was reversed by the treatment of test extracts and gliclazide. HbA1C levels were higher in the STZ-induced diabetic rats compared to the normal control rats. Treatment with ME-CMRH (methanol eluate – CMRH) decreased the HbA1C level of the STZ induced diabetic rats. Antidiabetic activity of the test extract ME-CMRH at 500 mg/kg body weight dose was comparable with the effect produced by the standard drug gliclazide in restoring the levels of blood glucose, body weight and HbA1C towards normal levels. The above data are indicative of the antidiabetic potential of C. macrophylla roots. In the evaluation of mechanism of action by gene expression studies, upregulation of Glut-4 as well as PPAR-γ were demonstrated.

scholarly journals In vitro Membrane Stabilizing and In vivo Analgesic Activities of Boehmeria glomerulifera Miq. in Swiss-Albino Mice Model

2016 ◽  
Vol 19 (2) ◽  
pp. 185-189
Author(s):  
Sabikunnahar Papia ◽  
Muhammed Mahfuzur Rahman ◽  
Md Mustafezur Rahman ◽  
Mohaiminul Adib ◽  
Mohammad Firoz Khan
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Hypotonic Solution ◽  
Mice Model ◽  
Immersion Method ◽  
Tail Immersion ◽  
Crude Methanol Extract

Boehmeria glomerulifera Miq. is an indigenous plant of Bangladesh. Traditional healers use this plant to treat various diseases. The crude methanol extract of whole plant of B. glomerulifera and its Kupchan fractions were screened for in vitro membrane stabilizing and only the crude extract at doses of 200- and 400- mg/kg body weight were screened for analgesic activity in mice model in order to establish the medicinal values of this plant. The membrane stabilizing activity of the extractives was evaluated by the ability of test materials to inhibit hypotonic solution- and heat-induced haemolysis of human erythrocytes. Moreover, the analgesic activity of methanol extract was evaluated by acetic acid induced writhing method and tail immersion method in mice. In hypotonic solution- and heat-induced conditions, the crude methanol extract showed inhibition of haemolysis by 64.80±0.34% and 21.63±0.76%, respectively as compared to 77.74±0.67% and 40.41±0.69% demonstrated by the standard, acetyl salicylic acid. On the other hand, the analgesic activity was determined for its peripheral and central pharmacological responses using acetic acid-induced writhing test and tail immersion method at doses of 200- and 400- mg/kg body weight. The extract reduced the acetic acid-induced writhings significantly (p < 0.05) in a dose dependent manner with the highest activity observed at 400 mg/kg (46.0%) body weight when compared to that of Diclofenac sodium (64.0%), as the standard drug. A significant increase (p < 0.01) of latency period was also found in tail immersion method. Therefore, the present study established that the methanol extract of B. glomerulifera possesses moderate membrane stabilizing and significant analgesic activities.Bangladesh Pharmaceutical Journal 19(2): 185-189, 2016

Analgesic Activity of the Rhizomes of Curcuma zedoaria

1970 ◽  
Vol 3 (1) ◽  
Author(s):  
Mohammad Shawkat Ali ◽  
ASM Masum ◽  
Sitesh C Bachar ◽  
Md. Saiful Islam
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Oral Administration ◽  
Analgesic Activity ◽  
Full Text ◽  
Significant Activity ◽  
Curcuma Zedoaria ◽  
Methanol Extracts ◽  
Successive Extraction ◽  
Activity Screening

Successive extraction of the dried rhizomes of Curcuma zedoaria Rosc. provided hexane, chloroform and methanol extracts. In analgesic activity screening, the chloroform and methanol extracts showed significant activity on Swiss albino mice. Oral administration of the crude extracts at a dose of 400 mg/kg body weight, exhibited 29.5 and 38.0 % inhibition of acetic acid induced writhing in mice, respectively while the hexane extract showed weak analgesic activity in the experiment. Key words: Curcuma zedoaria, Zingiberaceae, Analgesic activity. Dhaka Univ. J. Pharm. Sci. Vol.3(1-2) 2004 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals in vivo antibacterial and therapeutic properties of P. ostreatus against Staphylococcus aureus

2021 ◽  
Vol 9 (3) ◽  
pp. 273-281
Author(s):  
B.A. Oyekanmi ◽  
A.K. Onifade ◽  
I.B. Osho ◽  
O.T. Ajayi
Keyword(s):  
Staphylococcus Aureus ◽  
Pleurotus Ostreatus ◽  
Methanol Extract ◽  
Bacterial Count ◽  
Blood Parameters ◽  
Negative Control ◽  
Albino Rats ◽  
World Population ◽  
Wistar Albino Rats

Objectives: The use of natural products is embraced by a larger percentage of the world population. Most species of fungi including mushrooms produce useful secondary metabolites that stimulate the immune system against infection and diseases. Investigations were conducted to assay the therapeutic potentials of P. ostreatus against pathogenic staph infection.Methods: The methanol extract of P. ostreatus was prepared using the cold extraction method. Thirty Wistar albino rats weighing 82.0 g to 99.2 g were distributed into 6 groups of 5 and inoculated orally with actively growing Staphylococcus aureus suspension. P. ostreatus methanol extract LD > 5000 mg/kg 50 was used to determine the graded doses for the study. Graded doses of the extract 625 mg, 1250 mg, and 2500 mg were administered orally to the experimental animals for seven days.Results: The negative control and 625 mg had skin ulceration while 1250 mg to 2500 mg produced apparently healthy skin. Bacterial count after 7 days post-treatment was significantly high in the negative control and 625 mg dose (32.00 x 104 ± 6.10b; 43.40 x 104 ± 6.20b Cfu/ml) P< 0.05. Haematological and serum biochemical values were not significantly P < 0.05 affected. Pleurotus ostreatus administration at 1250 to 2500 mg produced a statistically low colony count that was comparable with 13.33 mg Ciprofloxacin and placebo.Conclusion: Pleurotus ostreatus at 1250 to 2500 mg was able to produce clinical recovery from S. aureus infection while 625 mg could not. The extract had no deleterious effect on the blood parameters, liver enzymes, and kidney biomarkers.  

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