scholarly journals ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES ON FIXED RETAINER ADHESIVE TOWARD TREPONEMA DENTICOLA

Author(s):  
JESSICA SANTOSO ◽  
MARIA PURBIATI ◽  
KRISNAWATI .

Objective: The objective of this study was to develop an antibacterial flowable composite containing silver nanoparticle (AgNP) for the prevention ofperiodontal disease in fixed retainers.Methods: About 1% AgNP was incorporated into a commercial composite (tetric flow). The experimental and control products were used to bondfixed retainers to 28 extracted mandibular first premolars. The samples were randomly divided into four groups (n=7): Premolar bonded withfiber-reinforced composites and tetric flow (F1); premolar bonded with fiber-reinforced composites and AgNP-enhanced tetric flow (F2); premolarbonded with multistranded wires (MW) and tetric flow (M1); and premolar bonded with MW and AgNP-enhanced tetric flow (M2). Each sample wassubmerged in a test tube containing bacterial Treponema denticola solution and was incubated for 24 h and 37°temperature. The bacterial colony ineach group was counted and analyzed.Results: This study showed that there was significant difference of T. denticola colony count between groups with and without AgNP-enhancedcomposites in both types of retainers.Conclusion: AgNP-enhanced flowable composites reduce the bacteria T. denticola colony count and possibly inhibit periodontal disease.

2014 ◽  
Vol 18 (2) ◽  
pp. 153-157
Author(s):  
Siti Sunarintyas ◽  
Widowati Siswomihardjo ◽  
Dyah Irnawati ◽  
Jukka Pekka Matinlinna

Matrix system used bis-GMA was reported hazardously. An alternative monomer such as 1,6-hexanediol dimethacrylate(HDDMA) was under research. The aim of this study was to evaluate residual monomer content of HDDMA basedmatrix compositions on FRCs (fiber-reinforced composites). Three monomers based on bis-GMA (Sigma-Aldrich,USA),methylmethacrylate (MMA, ProSciTech, Australia), HDDMA (Esstech, USA) were used and also camphorquinone (CQ,Esstech, USA), N,N-cyanoethyl methylaniline (CEMA, Esstech, USA), E-glass fibers (StickTech, Finland). The matrixratios (weight %) were 78.4% bis-GMA+19.6% MMA+1.0% CQ+1.0% CEMA (control-group), 78.4% HDDMA+19.6% MMA+1.0% CQ+1.0% CEMA (EXP-1group), and 49.0% HDDMA+49.0% MMA+1.0% CQ+1.0% CEMA(EXP-2group). Samples with fibers embedded in matrix were light-cured then powdered. Powder of 150mg was dilutedin acetonitrile to 10ml. The solution was filtered then injected into HPLC (20µL). Residual monomer content wasevaluated by mobile phase of acetonitrile and water of 7:3, flow rate 1mL/minute. The size of column was C185µ,125mm length, 4mm diameter. UV detection used 275nm. Data were analyzed by ANOVA. The result showed theaverage of residual monomer content (in %) was: 0.02125±0.00018 (control-group), 0.01660±0.00016 (EXP-1group),0.01676±0.00033 (EXP-2group). The ANOVA showed significant difference of monomer content among the groups(p<0.05). The LSD showed significant difference between EXP-1 and control-groups; also between EXP-2 and controlgroups (p<0.05). There was no significant difference between EXP-1 and EXP-2 groups (p>0.05). In conclusion, a resinmatrix system based on HDDMA–MMA (EXP-1 and EXP-2 groups) revealed significant difference of residualmonomer content to bis-GMA–MMA (control-group) system. The HDDMA-MMA matrix system had less residualmonomer content than bis-GMA-MMA.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Seyedeh Sareh Hendi ◽  
Negin Amiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  

This study investigates the antibacterial effects of erbium chromium laser at 2780 nm, silver nanoparticles, and erbium chromium along with silver nanoparticles on Enterococcus faecalis in comparison with sodium hypochlorite. In the present study, 90 extracted human single-rooted teeth were selected and standardized to a length of 15 mm. The canals were prepared by V-taper Gold rotary files and then incubated with E. faecalis for 21 days. The samples were divided into four experimental groups including hypochlorite sodium, silver nanoparticle, erbium chromium laser, and erbium chromium laser along with silver nanoparticle groups. Results showed that there was a significant reduction in colony count for all groups after interventions. Moreover, there was a significant reduction in the colony count for sodium hypochlorite group in comparison with another groups, and this group showed the highest reduction of colony count. There was a significant difference between silver nanoparticles and erbium chromium laser groups in colony count. According to the results, the silver nanoparticles offered strong antibacterial effects on E. faecalis and therefore can decrease bacterial colonies, while the use of the laser, despite the reduction of the bacterial colony, could not be sufficiently used for disinfection of root canal system.


2018 ◽  
Vol 2 (1) ◽  
pp. 29-35
Author(s):  
Widya Puspita Sari ◽  
Siti Sunarintyas ◽  
Nuryono Nuryono

E-glass fiber merupakan fiber reinforcement yang sering digunakan di kedokteran gigi karena berikatan baik dengan polimer melalui silane coupling agent, meningkatkan kekuatan mekanis dan tidak sensitif terhadap kelembaban. Ketersediaan E-glass fiber dental di Indonesia terbatas dengan harga relatif mahal. Glass fiber non dental banyak tersedia di Indonesia dengan harga terjangkau, yang biasa digunakan pada pembuatan panel gypsum dan komponen otomotif. Tujuan penelitian ini adalah mengetahui pengaruh komposisi glass fiber non dental terhadap kekuatan fleksural fiber reinforced composites. Bahan yang digunakan dalam penelitian ini adalah E-glass fiber dental (Fiber-splint, Polydentia SA, Switzerland), glass fiber non dental A (LT, China), B (CMAX, China) dan C (HJ, China), flowable composite (CharmFil Flow, Denkist, Korea) dan silane coupling agent (Monobond S, Ivoclar Vivadent, Liechtenstein). Subjek dibagi dalam 5 kelompok: tanpa fiber, E-glass fiber dental, glass fiber non dental A, B dan C . Uji kekuatan fleksural berdasarkan ISO 10477. Hasil yang diperoleh dianalisis menggunakan ANAVA satu jalur. Hasil penelitian menunjukkan rerata kekuatan fleksural (MPa) terendah pada kelompok tanpa fiber (126,18±5,21) dan tertinggi pada kelompok glass fiber non dental A (208,03±8,32). Hasil ANAVA satu jalur menunjukkan pengaruh bermakna dari komposisi fiber terhadap kekuatan fleksural (α < 0,05). Kesimpulan penelitian: Kandungan SiO2, Al2O3 dan alkali tanah oksida (MgO dan CaO) yang tinggi pada glass fiber meningkatkan kekuatan fleksural dari fiber reinforced composites.


Author(s):  
Divya Abraham ◽  
Vinitha Ravindran ◽  
Vinsley Rose ◽  
Joy Sarojini Michael

Objective: The main objective of the study was to determine whether bathing with 2% chlorhexidine or 0.9% saline or standard soap and water will reduce the bacterial colony count on skin effectively.Methods: Quantitative approach with experimental design and consecutive sampling was used. The study was conducted among 102 children admitted in pediatric wards of Christian Medical College, Vellore. Swabs from axilla and groin were collected at 0 h, 2 h, and 24 h of intervention to determine the bacterial colony count in subjects.Results: The results showed that, of 102 children, 73 (71.56%) of them had high axillary colony count and 69 (67.64%) of them had high groin colony count during admission. Majority 88.2% and 78% of them had colonization with coagulase-negative staphylococcus in the axilla and groin, respectively. There was no significant difference in the bacterial colony count in axilla at 2 and 24 h in all three groups. There was a significant difference in the bacterial colony count in groin at 2 h after the intervention in the chlorhexidine group, and the difference was not significant at 24 h. The colony count was not significantly different at 2 h post-intervention in saline and standard soap and water group but was statistically different in these groups at 24 h after the intervention.Conclusion: Chlorhexidine has shown to be effective for a shorter duration and it can be adopted for children who are undergoing invasive procedures and preoperatively in surgical wards while continuing routine standard bath for all children which is considered to be cost effective too.


Author(s):  
Sara Arastoo ◽  
Azam Behbudi ◽  
Vahid Rakhshan

Objectives: Pit and fissure sealants are recognized as an effective preventive approach in pediatric dentistry. Composite resin is the most commonly used sealant material. Adding nanoparticles to composite resin could result in production of flowable composite with higher mechanical properties and better flowability than previous sealants. This study aimed to compare the microleakage of a flowable nanocomposite and materials conventionally used as pit and fissure sealants. Materials and Methods: A total of 185 extracted mandibular third molar teeth were selected and randomly divided into 5 groups (n=36): flowable nanocomposite, flowable composite, filled sealants, nano-filled sealants, and unfilled sealants. Five teeth were reserved for examination under a scanning electron microscope (SEM). The samples were thermocycled (5-55°C, 1-minute dwell time) for 1000 cycles and immersed in 0.2% fuchsine solution for 24 hours. Teeth were sectioned buccolingually. Microleakage was assessed qualitatively and quantitatively by means of dye penetration and SEM. Data were analyzed using chi-square, Kruskal-Wallis, and Bonferroni-corrected Mann-Whitney U tests. Results: Qualitative microleakage assessment showed that flowable composite and nanofilled flowable composite had almost no microleakage (P<0.001). Regarding quantitative scores, the nanofilled flowable composite and unfilled fissure sealant showed the lowest and the highest rate of microleakage, respectively. No statistically significant difference was found between the two flowable composites (P=0.317). Filled resin-based sealant had significantly lower microleakage than unfilled resin-based sealant (P<0.001). Conclusion: Use of flowable and nanofilled flowable composites (but not unfilled resin-based fissure sealant) is recommended for sealing of pits and fissures of molars.


2013 ◽  
Vol 14 (4) ◽  
pp. 573-577 ◽  
Author(s):  
S Sujatha Gopal ◽  
B Shiva Kumar ◽  
P Spoorti ◽  
Jeetender Reddy ◽  
Jayaprakash Ittigi

ABSTRACT Aim Aim of this in vitro study was to evaluate the resistance to fracture of vertically fractured and reattached fragments bonded with fiber-reinforced composites. Materials and methods Root canals of 45 teeth were prepared, and the teeth were intentionally fractured into two separate fragments. Control groups (n = 15 each) consisted unfractured teeth with instrumented and obturated. Fractured teeth were divided into three groups (n = 15) and were attached using (1) dual-cure resin cement (RelyX U100), (2) dual-cure resin cement and polyethylene fiber (Ribbond), (3) dual-cure resin cement and glass fibers (stick-net). Force was applied at a speed of 0.5 mm/min to the root until fracture. Results and statistical analysis Group 1 (RelyX U100 group) demonstrated lowest fracture resistance. Group 4 (control group) showed highest fracture resistance followed by group 2 (Ribbond group) and group 3 (Stick-Net groups). Statistically no significant difference was there between groups 2, 3 and 4. Conclusion Vertically fractured teeth can be treated by filling the root canal space with dual-cure adhesive resin cement or by adding polyethylene fiber or glass fiber to increase the fracture resistance of the reattached tooth fragments, an alternative to extraction. How to cite this article Kumar BS, Spoorti P, Reddy J, Bhandi S, Gopal SS, Ittigi J. Evaluation of Fracture Resistance of Reattached Vertical Fragments Bonded with Fiber-reinforced Composites: An in vitro Study. J Contemp Dent Pract 2013;14(4):573-577.


2017 ◽  
Vol 2 (1) ◽  
pp. 13
Author(s):  
Ariyani Faizah ◽  
W. Widjijono ◽  
N Nuryono

The effect of composition glass fiber non dental on water solubility of fiber reinforced composites. E glass fiber dental is one of the most used dental fibers in several applications in the dental  field. However, the available of E glass fiber dental in Indonesia is very limited. A variety of types of non-dental glass fiber material is easily found as the materials engineering. The purpose of the study was to evaluate the effect of composition non dental glass fiber on the component solubility of FRC. The materials used in the research was E glass fiber dental (Fiber splint, Polydentia SA, Switzerland), composition A non-dental glass fiber (LT, China), composition B (CMAX, China), composition C (HJ, China), flowable composite (Charmfill Flow, Denkist, Korea) and silane coupling agent (Monobond S, Ivoclair Vivadent, Liechtenstein). The subject was divided into 4 groups. Component solubility test was based on the ISO 4049. The result was then analyzed with one way ANOVA (α=0,05). The result of the research showed that on the average percentage of the solubility (%), the lowest was on the group of E glass fiber dental (0.476±0.03) and the highest was on the non dental glass fiber C (0.600±0.01). The result of the one way ANOVA test showed a significant difference between the compositiom fiber on the component solubility. The conclusion the research was that low content of Na2O K2O, CaO and MgO decreased the component solubility of FRC.ABSTRAKE glass fiber dental adalah fiber yang sering digunakan di kedokteran gigi. Ketersediaan E glass fiber di Indonesia masih sangat terbatas. Berbagai jenis bahan glass fiber non dental banyak ditemukan dipasaran sebagai material engeenering dengan harga yang relatif murah sehingga diharapkan dapat digunakan sebagai alternatif pengganti E glass fiber dental. Komposisi glass fiber non dental hampir sama dengan E glass fiber dental. Komposisi berpengaruh terhadap sifat mekanis dan sifat-sifat kimia fiber. Komposisi glass fiber seperti Na2O dan K2O akan meningkatkan ketahanan terhadapap air. Tujuan dari penelitian ini adalah mengetahui pengaruh komposisi glass fiber non dental terhadap kelarutan komponen. Bahan yang digunakan dalam penelitian ini adalah E glass fiber dental (Fiber-splint, Polydentia SA, Switzerland), glass fiber non dental komposisi A (LT, China), komposisi B (CMAX, China), komposisi C (HJ, China), flowable komposit (CharmFill Flow, Denkist, Korea) dan silane coupling agent (Monobond S, Ivoclar Vivadent, Liechtenstein). Subjek dibagi dalam 4 kelompok untuk dilakukan uji kelarutan berdasarkan ISO 4049. Hasil yang diperoleh dianalisis menggunakan ANAVA satu jalur (a = 0,05). Hasil penelitian menunjukkan rerata kelarutan komponen (%) yang terendah pada kelompok E-glass fiber dental (0,476±0,03) dan hasil tertinggi pada glass fiber non dental C (0,600±0,01). Hasil uji Anava satu jalur menunjukkan perbedaan yang bermakna antara komposisi fiber pada kelarutan komponen (p<0,05). Kesimpulan penelitian adalah komposisi Na2O dan K2O serta CaO dan MgO yang rendah dapat menurunkan sifat kelarutan komponen dari fiber reinforced composites.


Author(s):  
Mohammadreza Moaddeli ◽  
Abdolmehdi Araghizadeh ◽  
Ehsan Shabani

Introduction: Cumin (Nigella Sativa) seed oil extract has some ingredients which have antimicrobial effects. The essential oils present in cumin act as antimicrobial agent and it influence on different type of Gram-negative and Gram-positive bacteria and also viruses, parasites and fungi. This study aimed to investigate the antimicrobial properties of cumin extract in disinfecting dentistry surfaces. Material and Methods: This study was performed experimentally and had three groups of cumin extract, Deconex and control group. For each of these groups, 12 culture media were prepared and we counted the colonies created in 24 hours and 48 hours and significance level was assessed using SPSS software and t-test.    Results: At 24 hours, there was a significant difference between the bacterial colony counts of the petri dishes from Cumin Seed (Nigella Sativa) Oil Extract at 5.83 and the Deconex at 0. And at 48 hours, there was also a significant difference since the bacterial colony count on the petri dishes with Cumin (Nigella Sativa) Oil Extract was too many to count and a 0.83 bacterial colony count for the petri dishes with the Deconex. Conclusion: The Cumin (Nigella Sativa) seed oil extract is not suitable to use as an alternative disinfectant of dental surfaces lonely. But some of its ingredients such as thymoquinone and hydroquinone can be used to produce a disinfecting solution.


2020 ◽  
Vol 11 (2) ◽  
pp. 181-186
Author(s):  
Tara Zarabian ◽  
Saba Azadi Mood ◽  
Nazanin Kiomarsi ◽  
Homa Noorollahian ◽  
Neda Hakimiha

Introduction: Advances have been made in the composition of flowable composites in recent years and self-adhering composites, which do not require a bonding agent, have recently been introduced to the market. This study aimed to assess the microshear bond strength (µSBS) of a self-adhering flowable composite (Vertise) to primary enamel treated with a graphite disc with silicon carbide particles (SIC) and laser irradiation, the result of which was compared to that of a conventional flowable composite (Premise). Methods: In this in vitro, experimental study, 72 samples of sound primary enamel were evaluated. A smooth enamel surface was obtained using a graphite disc. Next, the erbium chromium yttrium scandium gallium garnet (Er,Cr:YSGG) laser was used for enamel surface treatment in half of the samples (n=36). All the samples were then randomly divided into 4 groups of (i) Premise flowable composite (PF) without laser (n=18), (ii) Vertise flowable composite (VF) without laser (n=18), (iii) PF with laser (n=18), and (iv) VF with laser (n=18). The teeth were then incubated at 37°C for 24 hours and were then subjected to thermocycling. The µSBS of samples was measured using a universal testing machine and reported in megapascal (MPa). Data were analyzed using SPSS via the two-way ANOVA and independent-samples t test at P<0.05. Results: The mean µSBS of VF was significantly higher to the laser-treated samples (13.60±5.47) compared with the non-treated samples (5.89±2.42) (P<0.001). However, no significant difference was noted in the µSBS of PF to the laser-treated (13.18±3.45) and non-treated samples (16.06±3.52) (P=0.058). Conclusion: The µSBS of the conventional flowable composite is higher than that of the selfadhering flowable composite to the enamel of primary teeth. Enamel surface treatment with laser irradiation increases the µSBS of self-adhering flowable composites.


2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Mahtab Ebrahimi Nezhad ◽  
Dana Jafarpour ◽  
Mahdi Gholamrezaei Saravi

Objective: The present study aimed to assess the influence of vibration effect on microshear bond strength (µSBS) of flowable composite to enamel. Material and Methods: Sixty non-carious extracted human premolar teeth were collected and randomly divided into six groups (n = 10) after being trimmed to produce a smooth flat surface: Flowable composites [Wave (SDI), Wave HV (SDI) and Grandioflow (Voco)] were used as bonding agents without or with vibration using an ultrasonic scaler (Mini Piezon, EMS, Switzerland). Composite resin, with an internal diameter of 0.7mm and height of 1mm, was cured on the substrate. The specimens’ µSBS was tested by a microtensile tester (Bisco, USA) at a crosshead speed of 0.5 mm/min. The bond strength values were analyzed using one-way ANOVA and post hoc Tukey test (p < 0.05). Results: Vibration did not lead to any significant difference in the µSBS values of Wave, Wave HV, and Grandio Flow µSBS values (P=0.690, P=1.000 and P=0.947, respectively). No significant difference was found between flowable composites in terms of micro shear bond strength to enamel (p > 0.05). Conclusions: The application of ultrasonic vibration might not be advantageous in terms of improving the shear bond strength of flowable composites to enamel. KEYWORDS Bond strength; Enamel; Flowable composite; Ultrasonic; Vibration.


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