scholarly journals OPTIMIZATION OF MICROWAVE- AND ULTRASONIC-ASSISTED EXTRACTION OF MAHKOTA DEWA (PHALERIA MACROCARPA [SCHEFF.] BOERL.) FRUIT PULP

2020 ◽  
pp. 32-37
Author(s):  
ROSITA HANDAYANI ◽  
ANDRE BANGUN ◽  
PASKAH DEWI DEBORAH ◽  
ABDUL MUN’IM
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Water Addition ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Objective: This work aimed to determine the optimal extraction conditions of Mahkota dewa using different extraction methods: microwave-assistedextraction with ethanol (MAE-ethanol), ultrasonic-assisted extraction using a choline chloride natural deep eutectic solvent (UAE-NADES), andSoxhlet and reflux extractions.Methods: Experimental design was performed through Box-Behnken. The efficiency of the methods was evaluated based on the total phenolic content(TPC) and antioxidant activity. The presence of relevant compounds was qualitatively assessed by high-performance liquid chromatography (HPLC).Results: For the MAE-ethanol method, optimum extraction was achieved with 65% ethanol, in 1 min under 30% microwave power, and a 1:12 sample–solvent ratio, which yielded a TPC and antioxidant activity of 62.79±0.74 mg gallic acid equivalents (GAE)/g powder and 30.48±0.32%, respectively. InUAE-NADES, the optimal conditions were choline chloride: lactic acid ratio of 1:4 with a 50% water addition, 20 min extraction (TPC and antioxidantactivity of 65.25 mg GAE/g powder and 26.45±0.02%). The Soxhlet and reflux methods gave a TPC of 49.68±0.59 and 50.98±0.54 mg GAE/g powder,and antioxidant activity of 22.87±0.13% and 25.97±0.34%, respectively. Qualitative HPLC analysis showed the presence of quercetin, mahkoside A,4,6-dihydroxy-4-methoxybenzophenone-2-O-β-D-glucopyranoside, and mangiferin.Conclusion: MAE-ethanol was more efficient than the conventional Soxhlet and reflux methods and performed similarly to UAE-NADES in terms of TPC.

scholarly journals ULTRASONIC-ASSISTED EXTRACTION USING A BETAINE-BASED NATURAL DEEP EUTECTIC SOLVENT FOR RESVERATROL EXTRACTION FROM MELINJO (GNETUM GNEMON) SEEDS

2020 ◽  
pp. 26-31
Author(s):  
WIDYA DWI ARYATI ◽  
KHUSNA MILLATI AZKA ◽  
ABDUL MUN’IM
Keyword(s):  
Lactic Acid ◽  
High Performance ◽  
Deep Eutectic Solvent ◽  
High Water Content ◽  
Solvent Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Gnetum Gnemon ◽  
Ultrasonic Assisted Extraction ◽  
Natural Deep Eutectic Solvent

Objective: Melinjo (Gnetum gnemon L.) seeds are known to contain resveratrol, which are classified as a phenolic compound of the stilbenoid. Melinjoseeds have high water content, making them unstable to be stored for prolonged periods with open packaging at room temperature. The present studyaimed to explore the use of ultrasonic-assisted extraction with a betaine-based natural deep eutectic solvent (NADES) for resveratrol extraction fromirradiated melinjo seeds.Methods: The best betaine-based NADES component was identified among betaine-urea, betaine-lactic acid, and betaine-malic acid. Optimization ofextraction methods was performed using the best NADES and extraction variables such as time of extraction, water percentage, and sample: solventratio. The outcome of extraction was evaluated by measuring resveratrol content using high-performance liquid chromatography and the results wereanalyzed using response surface methodology.Results: The best betaine-based NADES was found to be betaine-lactic acid, yielding a resveratrol content of 0.3344 mg/g powder. The optimumextraction was achieved in 10 min with 60% water and a sample: solvent ratio of 1:10, yielding a resveratrol content of 0.227 mg/g powder.Conclusion: Betaine-based NADES can be purposed as an alternative solvent for resveratrol extraction from irradiated melinjo seeds.

scholarly journals Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Janieire Lorraine da Rocha Pittarello ◽  
Marcel Petreanu ◽  
Valdir Cechinel Filho ◽  
Clóvis Antonio Rodrigues ◽  
Luiz Carlos Klein-Júnior ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Solvent Ratio ◽  
Flavonoid Content ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Addition Level

Recently, it has been demonstrated that a rare flavonoid, 5,7-dihydroxy-6,8,4′-trimethoxyflavonol (1), isolated from the leaves of Rubus rosifolius showed significant antiproliferative activity against different cancer cell lines and had a diuretic effect. This study aimed to determine the best extraction method evaluating dynamic maceration extraction (DME), ultrasonic assisted extraction (UAE), and microwave assisted extraction (MAE). In addition, level optimization for different factors was focused using Design of Experiments. The flavonoid content was estimated by high performance liquid chromatography (HPLC). The results indicated that UAE provided the highest content with the shortest extraction time. For optimization, response surface methodology (RSM) was used, applying a Box-Behnken Design (BBD) with three factors at three levels. It was possible to observe that the flavonoid content significantly increased with the increase of temperature (max. 70°C). The time had little influence on the extraction and, for this reason, was set at 25 min. With regard to the drug/solvent ratio, it was observed that this had an impact on yield with a ratio of 3.5%. A model was built and a determination coefficient of 0.98 was obtained. After validation, an optimized method was developed, able to extract 1 with a lower time, lower solvent consumption, and affording higher contents.

scholarly journals Physicochemical, functional properties and antioxidant activity of protein extract from spent coffee grounds using ultrasonic-assisted extraction

2021 ◽  
Vol 6 (3) ◽  
pp. 864-878
Author(s):  
Namfon Samsalee ◽  
◽  
Rungsinee Sothornvit ◽  
Keyword(s):  
Antioxidant Activity ◽  
Functional Properties ◽  
Total Phenolic ◽  
Spent Coffee Grounds ◽  
Protein Extract ◽  
Assisted Extraction ◽  
Coffee Grounds ◽  
Spent Coffee Ground ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

<abstract> <p>Spent coffee grounds, the residue from coffee brewing, are still underutilized even though they contain several useful organic compounds including proteins. To valorize the spent coffee grounds, the spent coffee ground protein was investigated using ultrasonic-assisted extraction as a pretreatment to conventional extraction. The pretreatments involved different ultrasound amplitudes (40%, 60% and 80%) and extraction times (10, 20 and 30 min) and their effects on the physicochemical and functional properties including antioxidant activity of protein extract. It was found that the protein content extracted was increased approximately 2 times, compared to the initial spent coffee grounds. Furthermore, the ultrasonic-assisted extraction affected the physicochemical properties, functional properties and antioxidant activity of the protein extract. The 80% amplitude for 10 min extraction time improved the foaming capacity, foaming stability, emulsifying activity index and the emulsifying stability index of protein extract. The pretreatment at 20 min extraction time provided the highest antioxidant activity (933.92-976.03 mM Trolox eq/g protein extract) and the highest total phenolic content (267.66-304.81 mg GAE/g protein extract). Nonetheless, protein extract using ultrasonic-assisted extraction resulted in higher total phenolic content and antioxidant activity without changes in the protein structure as confirmed by changes in FT-IR spectra and SDS-PAGE profiles. Thus, the spent coffee ground protein can be an interesting and alternative plant protein with functional properties for food application. Moreover, this work showed the feasibility to reduce waste and the food waste valorization.</p> </abstract>

scholarly journals EXTRACTION OF PHENOLIC COMPOUNDS FROM PETAI LEAVES (PARKIA SPECIOSA HASSK.) USING MICROWAVE AND ULTRASOUND ASSISTED METHODS

2017 ◽  
Vol 6 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Buanasari Buanasari ◽  
Willy Tirza Eden ◽  
Ayu Ina Sholichah
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Natural Antioxidants ◽  
The Body ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ic50 Values

The antioxidant has an activity to neutralize free radical compound that the body needs to avoid damage cells and tissues. Phenolic is one of the compounds that have an antioxidant activity. The influences of ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) conditions on phenolic compounds of Parkia speciosa Hassk. leaves were investigated. The effects of temperature (40C, 50C, 60C and 70C), time (10, 30 and 50 minutes) and material-solvent ratio (1:10, 1:13, 1:15 ) were evaluated based on the yield, total phenolic content (TPC) and antioxidant activity. The result showed that the highest yield (15.82%) was obtained at 1:15 (w/w) of material-solvent ratio, 50C of temperature and 50 minutes of extraction time for MAE. The highest yield of UAE is 15.53% that sample was obtained at 1:13 (w/w) of material-solvent ratio, 60C of optimal temperature and 30 minutes extraction time. The highest IC50 of UAE method extract was 52.55 ppm, while the extract obtained using MAE method was 50.44 ppm. UAE is more stable at higher temperatures. Time and solvent which was used more efficient than MAE. Extract of petai leaves (Parkia speciosa Hassk.) were very potential to be used as a source of natural antioxidants because they have IC50 values from 41.39 to 66.00 ppm. Its antioxidants capacity is ranged from strong to very strong capacity.

scholarly journals Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 582
Author(s):  
Inês Mansinhos ◽  
Sandra Gonçalves ◽  
Raquel Rodríguez-Solana ◽  
José Luis Ordóñez-Díaz ◽  
José Manuel Moreno-Rojas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Industrial Applications ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvents ◽  
Ultrasonic Assisted

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.

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