scholarly journals THE PROTECTIVE EFFECT OF THE CARTHMUS TINCTORIUS LEAVES EXTRACTON METHOTREXATE-INDUCED PULMONARY FIBROSIS IN RATS

2019 ◽  
pp. 89-92
Author(s):  
HANAA SALMAN KHADHEM ◽  
MAHDI MTHUWAINI ◽  
WAJDY J. MAJID
Keyword(s):  
Pulmonary Fibrosis ◽  
Histopathological Examination ◽  
Relative Weight ◽  
Carthamus Tinctorius ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Lung Weight ◽  
Group 2

Objective: Pulmonary fibrosis (PF) is an irreversible and untreatable human disease encompasses a large group of chronic lung disorders associated with excessive remodeling, scarring, and fibrosis. The current work was designed to study the harmful effects of methotrexate (MTX) administration on the lung and the possible protective role of Carthmus tinctorius leaves extract. The animals were utilized in this study. Methods: A total of 40 male healthy adult Wistar albino rats with anaverage body weight of 200±25 g, were divided into four groups (10 animals each). G1: control group, G2: MTX group, G3: Carthamus tinctorius (CT), group G4:MTX+Carthamus tinctorius(CT). CT was administered orally at a dose of (40 mg/kg/day) for 4 w to G3 and G4. The (CT) group were performed to explore any toxic effect of the (CT) extract on the lung. Rats of G2 and G4 administered 4 mg/kg dose of MTX orally for 28 d. Rats of G1 were intraperitoneally (i. p) administered with normal saline 0.5 ml ∕ day for four weeks (4wk) to serveas control. The animals were weighed at the beginning, though, and at the end of experiments. Results: The study showed that the relative lung weight was significantly increased at (P˂0.01) in MTX-treated animals in comparison to the control group. A combination of CT extract with MTX revealed significant decrease (P<0.01) in the lung relative weight in comparison to MTX group. Histopathological examination revealed that lung injury was less severe in group 3 and 4compared to group 2. The results indicated that CT significantly decreased collagen deposition, hydroxyproline content, and ameliorated pathological changes. Conclusion: The study has clearly identified the importance protective role of CT extract on pulmonary fibrosis induced by methoxerate. We recommended CT as one of therapeutic strategy to amelioratethe lung fibrosis associated with methotrexate therapy.

Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

2011 ◽  
Vol 31 (6) ◽  
pp. 565-573 ◽  
Author(s):  
M Tutanc ◽  
V Arica ◽  
N Yılmaz ◽  
A Nacar ◽  
I Zararsiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Antioxidant Parameters ◽  
Group 2 ◽  
Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

scholarly journals Changes in Adult Rats’ Testis structure Induced by Hypothyroidism and Alleviating Role of L-Carnitine

2019 ◽  
Vol 1 (4) ◽  
pp. 13-28
Author(s):  
Abdelmonem Awad Hegazy ◽  
Manal Mohammad Morsy ◽  
Rania Said Moawad ◽  
Gehad Mohammad Elsayed
Keyword(s):  
Fatty Acids ◽  
Semen Analysis ◽  
Protective Role ◽  
The Body ◽  
Albino Rats ◽  
Adult Rats ◽  
Fatty Acids Metabolism ◽  
Group 3 ◽  
Group 2

Background Hypothyroidism is a metabolic disorder affecting the functions of many tissues in the body including the testis. Testis is rich in the polyunsaturated fatty acids content and lacks strong intrinsic antioxidant system making it prone to such oxidative stress. L-carnitine (LC) regulates long chain fatty acids metabolism; and is considered a valuable antioxidant factor. Aim It was to evaluate the effect of hypothyroidism induced by propylthiouracil (PTU) on rats’ testes and the possible protective role of LC. Methods Forty-eight adult male albino rats were used in this work. The animals were divided into three groups with sixteen animals in each. Group 1 (Control): Animals were kept without medications. Group 2 (PTU-treated): was subjected to administration of PTU; while group 3 (PTU and LC) received both PTU and LC. By the end of the experiment “30 days”, blood samples were taken for hormonal assay; then animals were anaesthetized and sacrificed. Specimens were homogenized for biochemical analysis; epididymal content of each rat was obtained immediately for semen analysis. Testes’ specimens were harvested, prepared and examined by light microscope examination. Results Induced hypothyroidism was noticed to cause histopathological, morphometric and biochemical changes in rat’s testes. LC protected the testicular specimens against such changes; it also improved the seminal quality and quantity as well as testicular structure and biochemistry. Conclusion Hypothyroidism could result in hazards to the structure of testis. Fortunately co-administration of LC might reduce such hazards.

scholarly journals Protective Effect of Boswellic Acids against Doxorubicin-Induced Hepatotoxicity: Impact on Nrf2/HO-1 Defense Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Bassant M. Barakat ◽  
Hebatalla I. Ahmed ◽  
Hoda I. Bahr ◽  
Alaaeldeen M. Elbahaie
Keyword(s):  
Day Treatment ◽  
Protective Role ◽  
Saline Control ◽  
Control Group ◽  
Boswellic Acids ◽  
Stress Markers ◽  
Inhibit Lipid Peroxidation ◽  
Group 2 ◽  
Altered Liver

The current study aimed to investigate the potential protective role of boswellic acids (BAs) against doxorubicin- (DOX-) induced hepatotoxicity. Also, the possible mechanisms underlying this protection; antioxidant, as well as the modulatory effect on the Nrf2 transcription factor/hem oxygenase-1 (Nrf2/HO-1) pathway in liver tissues, was investigated. Animals were allocated to five groups: group 1: the saline control, group 2: the DOX group, animals received DOX (6 mg/kg, i.p.) weekly for a period of three weeks, and groups 3–5: animals received DOX (6 mg/kg, i.p.) weekly and received protective doses of BAs (125, 250, and 500 mg/kg/day). Treatment with BAs significantly improved the altered liver enzyme activities and oxidative stress markers. This was coupled with significant improvement in liver histopathological features. BAs increased the Nrf2 and HO-1 expression, which provided protection against DOX-induced oxidative insult. The present results demonstrated that BAs appear to scavenge ROS and inhibit lipid peroxidation and DNA damage of DOX-induced hepatotoxicity. The antioxidant efficacy of BAs might arise from its modulation of the Nrf2/HO-1 pathway and thereby protected liver from DOX-induced oxidative injury.

scholarly journals Protective role of Nigella sativa in chemotherapy-induced alopecia

2017 ◽  
Vol 12 (4) ◽  
pp. 455
Author(s):  
Uzma Saleem ◽  
Shakila Sabir ◽  
Bashir Ahmad
Keyword(s):  
Nigella Sativa ◽  
Video Clip ◽  
Protective Role ◽  
Hair Follicles ◽  
Control Group ◽  
Albino Rats ◽  
Adult Rat ◽  
Anagen Phase ◽  
Full Screen

<p>Chemotherapy-induced alopecia affects the pathological as well as the psychological aspects of the cancer patient. In the present study, the protective role of Nigella sativa was evaluated in both adult and newborn albino rats. The anagen phase was first induced. N. sativa oil, N. sativa decoction (5%, 10% and 15%) and minoxidil lotion (standard) were applied daily to the rats two days after the depilation. During the anagen VI phase of the hair follicles, alopecia was induced by giving cyclophosphamide 125 mg/kg, ip to the adult rat and 50 mg/kg to the newborn rats. Cyclophosphamide-induced the alopecia to the whole depilated area of skin in adult rat while alopecia totalis was observed in the newborn rat disease control group. N. sativa oil, N. sativa decoction (5%) showed a significant protective effect against cyclophosphamide-induced alopecia. In conclusion, it is evident that N. sativa provides significant protection against chemotherapy-induced alopecia.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 43 sec:   <a href="https://www.youtube.com/v/AKhk27V3juE">Full Screen</a>   <a href="https://www.youtube.com/watch?v=AKhk27V3juE">Alternate</a></p>

Protective role of silymarin and D-penicillamine against lead-induced liver toxicity and oxidative stress

2017 ◽  
Vol 33 (6) ◽  
pp. 512-518 ◽  
Author(s):  
Seyedeh Missagh Jalali ◽  
Hossein Najafzadeh ◽  
Sadegh Bahmei
Keyword(s):  
Liver Toxicity ◽  
Serum Alkaline Phosphatase ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Antioxidant Defence ◽  
Antioxidant Defence System ◽  
Pb Exposure ◽  
Group 2

This study was performed to assess hepatotoxicity and alterations in liver antioxidant defence in acute lead (Pb) exposure and the protective effects of silymarin in comparison to D-penicillamine in rats. Forty eight Albino rats were divided in eight groups and received the following treatments in a 10-day experiment – group 1: normal saline as control; group 2: 25-mg/kg Pb acetate, intraperitoneally (IP) for the last 5 days; group 3: 100-mg/kg D-penicillamine, IP for the last 5 days; group 4: 200-mg/kg silymarin, orally for 10 days; and groups 5, 6, 7 and 8: in addition to Pb, they received D-penicillamine, for the last 5 days, silymarin for 10 days, a combination of silymarin for 10 days and D-penicillamine for the last 5 days and silymarin for the last 5 days, respectively. Pb acetate exposure induced significant elevation in serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities in group 2 compared to control group. Significant reductions in serum total protein and albumin in all Pb-exposed groups and in serum glucose in groups 2, 6 and 8 were also observed. Liver tissue superoxide dismutase and glutathione peroxidase were significantly lower in groups 2 and 8 compared to control group. Silymarin pretreatment and D-penicillamine administration in groups 5, 7 and 8 could significantly lower ALP, ALT and AST and improve liver antioxidant enzymes. Thus, acute Pb exposure induced hepatotoxicity with suppression of liver antioxidant defence system and silymarin, as an antioxidant could alleviate this effect.

scholarly journals Protective role of caffeine versus N-acetylcysteine in hyperoxic acute lung injury in neonatal rats

2017 ◽  
Vol 34 (02) ◽  
pp. 058-067
Author(s):  
A. Sadek ◽  
R. Khattab ◽  
A. Amer ◽  
A. Youssef
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Immune Reaction ◽  
Interleukin 1 ◽  
Protective Role ◽  
High Oxygen ◽  
Control Group ◽  
Albino Rats ◽  
Neonatal Rats

Abstract Introduction: Prolonged breathing of high oxygen concentration leads to hyperoxic acute lung injury. Neonatal Respiratory diseases usually require increased supplement of high oxygen concentrations, so neonates are more susceptible to hyperoxic acute lung injury. The aim of this work was to investigate the protective role of caffeine versus N-acetylcysteine against hyperoxic acute lung injury in neonatal rats. Materials and Methods: 32 albino rats aged seven days were used in this experiment. The pups were divided into four groups; 1) Control or normoxic group; rats placed in normoxic chamber where fraction of inspired oxygen (FiO2) was 0.21, 2) Hyperoxic group; rats were placed in hyperoxic chamber (FiO2>0.8) using an oxygen flow of 1.5 Litre/min, 3) Hyperoxia-CAF group; rats exposed to hyperoxia and received a single intra-peritoneal injection of 20 mg/kg caffeine just prior to exposure, and 4) Hyperoxia-NAC group; rats exposed to hyperoxia and received a single intra-peritoneal injection of 150 mg/kg N-acetylcysteine just prior to exposure. 48 hours after exposure, lung specimens were processed for histological and immunohistochemical study using caspase-3, cluster of differentiation-68-antibody (CD68) and interleukin-1-beta (IL-1β). Results: Neonatal hyperoxia led to severe impairment in lung architecture, with a highly significant increase in alveolar macrophages. Also, caspase and IL-1β immune-reaction were increased significantly as compared to control group. Caffeine could improve the histolopathological picture of hyperoxic acute lung injury, and also could decrease alveolar macrophage count and IL-1β immune-reaction better than N-acetylcysteine. Conclusion: Caffeine is more effective than N-acetylcysteine in prophylaxis against hyperoxic acute lung injury in neonates.

scholarly journals Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

2017 ◽  
Vol 104 (2) ◽  
pp. 139-149 ◽  
Author(s):  
M Savran ◽  
E Cicek ◽  
DK Doguc ◽  
H Asci ◽  
S Yesilot ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Histopathological Examination ◽  
Redox System ◽  
Protective Role ◽  
Hepatic Tissue ◽  
Control Group ◽  
Liver And Kidney ◽  
Vit C

Like several other anticancer drugs, methotrexate (MTX) causes side effects, such as neuropathic pain, hepatotoxicity, and nephrotoxicity. Abnormal production of reactive oxygen species has been suspected in the pathophysiology of MTX-induced hepatorenal toxicity. Therefore, the aim of this study was to investigate the probable protective role of vitamin C (Vit C) on oxidative stress induced by MTX in the liver and kidney tissues of rats. A total of 32 rats were randomly and equally divided into four groups. The first group served as the control group. The second group received a single dose of 20 mg/kg of MTX intraperitoneally. To demonstrate our hypothesis, the third and the fourth groups received 250 mg/kg of Vit C for 3 days by oral gavage, with or without MTX treatment. At the end of the study, the liver and kidney tissues of the rats were collected and examined using histology. Both the tissues were assayed for malondialdehyde concentration and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. In hepatic and renal tissues, lipid peroxidation levels were increased, whereas SOD, CAT, and GSH-Px levels were decreased by MTX. All parameters, including CAT levels in hepatic tissue, were significantly restored after the administration of Vit C for 3 days. Similar to the biochemical findings, evidence of oxidative damage was examined in both types of tissues by histopathological examination. From the results of this study, we were able to observe that Vit C administration modulates the antioxidant redox system and reduces the renal and hepatic oxidative stress induced by MTX. Vit C can ameliorate the toxic effect of MTX in liver and kidney tissues of rat.

scholarly journals Evaluation of the Curative and Protective Role of Fresh Chicory Juice in Treatment of Hepatic Fibrosis in Male Albino Rats

2021 ◽  
Vol 14 (3) ◽  
pp. 1331-1343
Author(s):  
Ahmed Algazeery ◽  
Ahmed H. Moustafa ◽  
Ashraf S. El-Sayed ◽  
Marwa G. Rizk ◽  
Norhan A. Sabbah
Keyword(s):  
Liver Fibrosis ◽  
Lipid Profile ◽  
Dna Fragmentation ◽  
Kidney Function ◽  
Histopathological Examination ◽  
Antimicrobial Properties ◽  
Protective Role ◽  
Albino Rats ◽  
Synthetic Drugs

Background: Using synthetic drugs for treating liver fibrosis remains a challenge since, in contrast to natural products, are remarkably expensive and associated with several adverse effects. Herbs and plants showed strong antioxidant, anti-inflammatory, and antimicrobial properties. Aim: To investigate the hepatoprotective role of fresh chicory juice in delaying the immune response of hepatic cells to Carbon tetrachloride [CCl4]-induced fibrosis. Methods: Fresh chicory plant juice [50%] was given instead of drinking water to male albino rats [150-200 g]. Blood samples were collected for biochemical evaluation of liver and kidney function, antioxidant markers, lipid profile, and gene expression of TGF-ß by quantitative real-time quantification polymerase chain reaction [q PCR]. Liver tissue was removed and subjected to histopathological and genomic DNA fragmentation assay. Results: Measurements of liver enzymes, kidney function, lipid profile and levels of antioxidants confirmed the ability of chicory to protect the liver against CCl4-induced liver fibrosis by acting as a good inhibitor of TGF-ß. These results were confirmed by histopathological examination and DNA fragmentation. Conclusion: Administration of fresh chicory juice [50%] showed a significant protective role of chicory plant in delaying CCl4-induced liver fibrosis by decreasing TGF-ß.

scholarly journals The effectiveness of the protective role of beetroot juice for the male reproductive system of albino rats against the toxicity of cadmium chloride

2019 ◽  
Vol 24 (1) ◽  
pp. 43
Author(s):  
Qasim Azeez Razooqi AL-Janabi ◽  
Rashid Khamees Shaban ◽  
Noor Ibrahem Hasan AL-Kraie
Keyword(s):  
Cadmium Chloride ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Beetroot Juice ◽  
Negative Effects ◽  
Fourth Group ◽  
Abnormal Sperm

This study was designed to evaluate the protective role of beetroot juice against the toxicity of cadmium chloride (CdCl2) in male albino rats. The study included 20 male albino rats ages ranged between (2-3 months) and weights ranged between (220-260g), which were randomly distributed into 4 groups, the first group that has been promised to control group was given distilled water, the second group was given beetroot juice 10 ml/kg b.w., group third was given at a dose of cadmium chloride 5 mg/kg  b.w. for 30 days, which promised an infected control, and the fourth group were treated beetroot juice 10 ml/kg b. w. with cadmium chloride. The results showed that the treatment of animals cadmium chloride led group to a significant decrease (P ≤ 0.05) in the hormonal serum, to negative effects on of the natural shape of the sperm and to negative effects cleared on histological in testes tissues compared with the control group, In general has dosage rats treatment cadmium chloride juice of beetroot, to the positive improvement for most of the values ​​of previous indicators has led to increase significantly, the concentration of the LH, FSH and Testosterone hormone , to an improvement in the natural shape of the sperm, decreased significantly in the form abnormal sperm, led to an improvement cleared tissue in these totals became approach with what has been observed in the control group.   http://dx.doi.org/10.25130/tjps.24.2019.007     

The protective role of curcumin against toxic effect of nonylphenol on bone development

2021 ◽  
pp. 096032712110305
Author(s):  
P Alısan Suna ◽  
O Cengız ◽  
A Ceyhan ◽  
E Atay ◽  
T Ertekin ◽  
...  
Keyword(s):  
Toxic Effect ◽  
Corn Oil ◽  
Bone Development ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Wistar Albino Rats ◽  
The Sacrifice

Introduction: In the study, it was aimed to investigate the possible protective effects of curcumin, a potent antioxidant, against the toxic effect of nonylphenol on bone development. Methods: Thirty pregnant female Wistar albino rats were used. The rats were randomly divided into the following five groups; the control group, corn oil group (150 µl/kg/day), nonylphenol group (50 µl/kg/day), curcumin group (100 mg/kg/day) and curcumin + nonylphenol group (100 mg/kg/day + 50 µl/kg/day). The doses were given by gavage from the 5th day to the 20th day of gestation. The fetuses were removed out on the 20th day of pregnancy by cesarean at the end of the study. After the sacrifice of the animals, double skeletal staining in front extremity (clavicula, scapula, humerus, radius, ulna) and hind extremity (femur, tibia, fibula), additionally histological and immunohistochemical examinations in femur bone were performed. Results: The nonylphenol group offspring have the lowest weights of fetuses and placenta, head-to-hip lengths, biparietal and occipitofrontal length, and also, bone length percentage and percentage of the ossification area in all measurements of the front extremity and hind extremity Interestingly, the groups treated with curcumin showed close to the control group in terms of double skeletal staining, histological, and immunohistochemical examinations. Conclusions: Our findings demonstrated an association between bone development and exposure to nonylphenol. The findings suggest that curcumin treatments may be effective in accelerating bone formation.

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