3D bioprinting for meniscus tissue engineering: a review of key components, recent developments and future opportunities

2021 ◽  
Author(s):  
Xavier Barceló ◽  
Stefan Scheurer ◽  
Rajesh Lakshmanan ◽  
Cathal J Moran ◽  
Fiona Freeman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Meniscal Repair ◽  
Spatial Patterning ◽  
3D Bioprinting ◽  
Research Areas ◽  
Engineered Tissues ◽  
Meniscal Tissue ◽  
Meniscus Tissue ◽  
Recent Developments

3D bioprinting has the potential to transform the field of regenerative medicine as it enables the precise spatial patterning of biomaterials, cells and biomolecules to produce engineered tissues. Although numerous tissue engineering strategies have been developed for meniscal repair, the field has yet to realize an implant capable of completely regenerating the tissue. This paper first summarized existing meniscal repair strategies, highlighting the importance of engineering biomimetic implants for successful meniscal regeneration. Next, we reviewed how developments in 3D (bio)printing are accelerating the engineering of functional meniscal tissues and the development of implants targeting damaged or diseased menisci. Some of the opportunities and challenges associated with use of 3D bioprinting for meniscal tissue engineering are identified. Finally, we discussed key emerging research areas with the capacity to enhance the bioprinting of meniscal grafts.

scholarly journals Cell-Free Strategies for Repair and Regeneration of Meniscus Injuries through the Recruitment of Endogenous Stem/Progenitor Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Weimin Guo ◽  
Wenjing Xu ◽  
Zhenyong Wang ◽  
Mingxue Chen ◽  
Chunxiang Hao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Progenitor Cells ◽  
Disease Transmission ◽  
Critical Role ◽  
Meniscal Repair ◽  
Vital Role ◽  
Meniscal Tissue ◽  
Increased Risk ◽  
Numerous Cell

The meniscus plays a vital role in protecting the articular cartilage of the knee joint. The inner two-thirds of the meniscus are avascular, and injuries to this region often fail to heal without intervention. The use of tissue engineering and regenerative medicine techniques may offer novel and effective approaches to repairing meniscal injuries. Meniscal tissue engineering and regenerative medicine typically use one of two techniques, cell-based or cell-free. While numerous cell-based strategies have been applied to repair and regenerate meniscal defects, these techniques possess certain limitations including cellular contamination and an increased risk of disease transmission. Cell-free strategies attempt to repair and regenerate the injured tissues by recruiting endogenous stem/progenitor cells. Cell-free strategies avoid several of the disadvantages of cell-based techniques and, therefore, may have a wider clinical application. This review first compares cell-based to cell-free techniques. Next, it summarizes potential sources for endogenous stem/progenitor cells. Finally, it discusses important recruitment factors for meniscal repair and regeneration. In conclusion, cell-free techniques, which focus on the recruitment of endogenous stem and progenitor cells, are growing in efficacy and may play a critical role in the future of meniscal repair and regeneration.

scholarly journals 3D Bioprinting Regulations: a UK/EU Perspective

2017 ◽  
Vol 8 (2) ◽  
pp. 441-447 ◽  
Author(s):  
Phoebe LI ◽  
Alex FAULKNER
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Medical Device ◽  
3D Bioprinting ◽  
Medicinal Products ◽  
Legal Regime ◽  
Advanced Therapy ◽  
Regulatory Instruments ◽  
The Eu ◽  
Medical Device Regulation

AbstractThis report introduces the challenges 3D bioprinting poses to the existing legal regime across bioethics, safety, regenerative medicine, and tissue engineering. We briefly review the 3D bioprinting technology and look into the relevant regulatory instruments for the pre-printing, printing, and post-printing stages. Special attention is paid to the applications of the EU Advanced Therapy Medicinal Products Regulation and the new Medical Device Regulation.

scholarly journals The Brazilian sectoral innovation system in the field of Tissue Engineering and Bioprinting: actors, challenges and perspectives

2019 ◽  
Vol 2 (1) ◽  
pp. 33
Author(s):  
Pedro Xavier Rodriguez Massaguer ◽  
Ana Luiza Garcia Massaguer Millás
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Regenerative Medicine ◽  
Innovation System ◽  
Innovation Systems ◽  
3D Bioprinting ◽  
System Framework ◽  
Medium Term ◽  
History Of ◽  
Systems Framework

The objective of this work is to map the main actors within the Brazilian innovation system framework in the field of tissue engineering and bioprinting, and analyze the main conditioners related to entrepreneurship and innovation. While keeping as a backdrop, the history of 3D Biotechnology Solutions startup, its challenges and projects. Tissue engineering is a subcategory of regenerative medicine with the purpose of repairing or substituting, partially or completely, tissues or organs that have been affected by some disease or lesion. The conventional methods used for the production of these biomaterials via tissue engineering do not have the capacity to mimic the reality of native structures in the nano, micro and macro scales, while guaranteeing the reproducibility andscalability ofthematerials. Technologies such as 3D bioprinting or additive manufacturing could change the way that many diseases are treated in the medium term by replacing the damaged tissues with custom bio-similar constructs. Mapping and reflections based on the innovation systems framework contribute to organize stimulus policies, stimulate interaction between actors, identify gaps and technological demands and periodically organize the analysis and expansion of this system in Brazil.

Nanomaterials for bioprinting: functionalization of tissue-specific bioinks

2021 ◽  
Author(s):  
Andrea S. Theus ◽  
Liqun Ning ◽  
Linqi Jin ◽  
Ryan K. Roeder ◽  
Jianyi Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Disease Modeling ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Significant Step ◽  
Printing Processes

Abstract Three-dimensional (3D) bioprinting is rapidly evolving, offering great potential for manufacturing functional tissue analogs for use in diverse biomedical applications, including regenerative medicine, drug delivery, and disease modeling. Biomaterials used as bioinks in printing processes must meet strict physiochemical and biomechanical requirements to ensure adequate printing fidelity, while closely mimicking the characteristics of the native tissue. To achieve this goal, nanomaterials are increasingly being investigated as a robust tool to functionalize bioink materials. In this review, we discuss the growing role of different nano-biomaterials in engineering functional bioinks for a variety of tissue engineering applications. The development and commercialization of these nanomaterial solutions for 3D bioprinting would be a significant step towards clinical translation of biofabrication.

scholarly journals Crosstalk between Substrates and Rho-Associated Kinase Inhibitors in Cryopreservation of Tissue-Engineered Constructs

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Arindam Bit ◽  
Awanish Kumar ◽  
Abhishek Kumar Singh ◽  
Albert A. Rizvanov ◽  
Andrey P. Kiassov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Kinase Inhibitors ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cells ◽  
Cellular Viability ◽  
Technical Limitation ◽  
Engineered Tissues

It is documented that human mesenchymal stem cells (hMSCs) can be differentiated into various types of cells to present a tool for tissue engineering and regenerative medicine. Thus, the preservation of stem cells is a crucial factor for their effective long-term storage that further facilitates their continuous supply and transportation for application in regenerative medicine. Cryopreservation is the most important, practicable, and the only established mechanism for long-term preservation of cells, tissues, and organs, and engineered tissues; thus, it is the key step for the improvement of tissue engineering. A significant portion of MSCs loses cellular viability while freeze-thawing, which represents an important technical limitation to achieving sufficient viable cell numbers for maximum efficacy. Several natural and synthetic materials are extensively used as substrates for tissue engineering constructs and cryopreservation because they promote cell attachment and proliferation. Rho-associated kinase (ROCK) inhibitors can improve the physiological function and postthaw viability of cryopreserved MSCs. This review proposes a crosstalk between substrate topology and interaction of cells with ROCK inhibitors. It is shown that incorporation of ionic nanoparticles in the presence of an external electrical field improves the generation of ROCK inhibitors to safeguard cellular viability for the enhanced cryopreservation of engineered tissues.

scholarly journals Recent Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: An Updated Review

2019 ◽  
Vol 20 (18) ◽  
pp. 4628 ◽  
Author(s):  
Kevin Dzobo ◽  
Keolebogile Shirley Caroline M. Motaung ◽  
Adetola Adesida
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Treatment Options ◽  
3D Bioprinting ◽  
Characterization Methods ◽  
Physical Strength ◽  
Decellularized Extracellular Matrix ◽  
The Right

The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix (ECM), cells, and inductive biomolecules. Regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. The coupling of regenerative medicine and tissue engineering with 3D printing, big data, and computational algorithms is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules, and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility, and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

scholarly journals An Update on Graphene-Based Nanomaterials for Neural Growth and Central Nervous System Regeneration

2021 ◽  
Vol 22 (23) ◽  
pp. 13047
Author(s):  
Maria Grazia Tupone ◽  
Gloria Panella ◽  
Michele d’Angelo ◽  
Vanessa Castelli ◽  
Giulia Caioni ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Smart Materials ◽  
Biological Properties ◽  
Neural Connections ◽  
Research Areas ◽  
Neural Growth

Thanks to their reduced size, great surface area, and capacity to interact with cells and tissues, nanomaterials present some attractive biological and chemical characteristics with potential uses in the field of biomedical applications. In this context, graphene and its chemical derivatives have been extensively used in many biomedical research areas from drug delivery to bioelectronics and tissue engineering. Graphene-based nanomaterials show excellent optical, mechanical, and biological properties. They can be used as a substrate in the field of tissue engineering due to their conductivity, allowing to study, and educate neural connections, and guide neural growth and differentiation; thus, graphene-based nanomaterials represent an emerging aspect in regenerative medicine. Moreover, there is now an urgent need to develop multifunctional and functionalized nanomaterials able to arrive at neuronal cells through the blood-brain barrier, to manage a specific drug delivery system. In this review, we will focus on the recent applications of graphene-based nanomaterials in vitro and in vivo, also combining graphene with other smart materials to achieve the best benefits in the fields of nervous tissue engineering and neural regenerative medicine. We will then highlight the potential use of these graphene-based materials to construct graphene 3D scaffolds able to stimulate neural growth and regeneration in vivo for clinical applications.

scholarly journals 3D Bioprinting at the Frontier of Regenerative Medicine, Pharmaceutical, and Food Industries

2021 ◽  
Vol 2 ◽  
Author(s):  
Qasem Ramadan ◽  
Mohammed Zourob
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Production Process ◽  
Paradigm Shift ◽  
3D Bioprinting ◽  
Printing Technology ◽  
Food Industries ◽  
3D Printing Technology ◽  
Key Driver

3D printing technology has emerged as a key driver behind an ongoing paradigm shift in the production process of various industrial domains. The integration of 3D printing into tissue engineering, by utilizing life cells which are encapsulated in specific natural or synthetic biomaterials (e.g., hydrogels) as bioinks, is paving the way toward devising many innovating solutions for key biomedical and healthcare challenges and heralds' new frontiers in medicine, pharmaceutical, and food industries. Here, we present a synthesis of the available 3D bioprinting technology from what is found and what has been achieved in various applications and discussed the capabilities and limitations encountered in this technology.

scholarly journals 3D Printed Poly(ε-Caprolactone)/Meniscus Extracellular Matrix Composite Scaffold Functionalized With Kartogenin-Releasing PLGA Microspheres for Meniscus Tissue Engineering

2021 ◽  
Vol 9 ◽  
Author(s):  
Hao Li ◽  
Zhiyao Liao ◽  
Zhen Yang ◽  
Cangjian Gao ◽  
Liwei Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Composite Scaffold ◽  
Prolonged Release ◽  
Plga Microspheres ◽  
Cell Vitality ◽  
Meniscal Tissue ◽  
Meniscus Tissue ◽  
Meniscus Regeneration

Meniscus tissue engineering (MTE) aims to fabricate ideal scaffolds to stimulate the microenvironment for recreating the damaged meniscal tissue. Indeed, favorable mechanical properties, suitable biocompatibility, and inherent chondrogenic capability are crucial in MTE. In this study, we present a composite scaffold by 3D printing a poly(ε-caprolactone) (PCL) scaffold as backbone, followed by injection with the meniscus extracellular matrix (MECM), and modification with kartogenin (KGN)-loaded poly(lactic-co-glycolic) acid (PLGA) microsphere (μS), which serves as a drug delivery system. Therefore, we propose a plan to improve meniscus regeneration via KGN released from the 3D porous PCL/MECM scaffold. The final results showed that the hydrophilicity and bioactivity of the resulting PCL/MECM scaffold were remarkably enhanced. In vitro synovium-derived mesenchymal stem cells (SMSCs) experiments suggested that introducing MECM components helped cell adhesion and proliferation and maintained promising ability to induce cell migration. Moreover, KGN-incorporating PLGA microspheres, which were loaded on scaffolds, showed a prolonged release profile and improved the chondrogenic differentiation of SMSCs during the 14-day culture. Particularly, the PCL/MECM-KGN μS seeded by SMSCs showed the highest secretion of total collagen and aggrecan. More importantly, the synergistic effect of the MECM and sustained release of KGN can endow the PCL/MECM-KGN μS scaffolds with not only excellent cell affinity and cell vitality preservation but also chondrogenic activity. Thus, the PCL/MECM-KGN μS scaffolds are expected to have good application prospects in the field of MTE.

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