scholarly journals Effects of Extended Aging on the Flavor Characteristics of Grass and Grain Fed Australian Beef Longissimus Thoracis

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
O. E. Musa ◽  
A. J. Garmyn ◽  
J. F. Legako ◽  
D. R. Woerner ◽  
M. F. Miller

ObjectivesThe objective of this study was to investigate the effects of extended wet ageing on the flavor characteristics, of grass and grain fed Australian beef lumborum thoracis.Materials and MethodsCube rolls (HAM #2244) were collected from grass and grain fed cattle (n = 30) at a commercial abattoir near Brisbane, Australia. Cube rolls were vacuum packaged and shipped under refrigeration (0–2°C) to Texas Tech University. Each cube roll was cut into 2.5-cm steaks and labeled according to position from posterior to anterior end. Steaks were vacuumed packaged, stored through the appropriate postmortem ageing period (35, 45, 55, or 65 d postmortem), and then frozen until further analysis. One steak from each cube roll was used for trained descriptive flavor analysis with 8 trained panelists comprised of mostly graduate students from Texas Tech University. Flavor attributes of cooked steaks were scored using 100-point anchored line scales (0 = none, 50 = moderate, and 100 = strong).Data were analyzed used PROC GLIMMIX of SAS with diet, postmortem ageing, and their interaction as fixed effects and panelist as a random effect. Final temperature was tested as a covariate for all the flavor attributes.ResultsAn interaction was detected only for the bitter flavor and overall juiciness (P ≤ 0.03). Beef flavor ID, fat-like, metallic, umami, and sweet were not influenced by diet or postmortem ageing (P > 0.05). Ageing influenced bloody serumy flavor (P > 0.05) with 45 d samples having greater flavor than 55 d samples, but not differing (P > 0.05) from any other ageing period. Diet and ageing influenced rancid flavor (P > 0.05), with grass fed samples having a stronger rancid flavor than grain fed samples. Samples aged 65 d had a stronger rancid flavor than 45 or 55-d samples, and 35-d samples had the lowest rancid flavor. Diet and ageing influenced grassy flavor (P > 0.05), again being stronger in grass than grain fed samples. Samples aged 35 d had a weaker (P > 0.05) grassy flavor than any other ageing period, which did not differ (P > 0.05). Diet and ageing had an effect on liver-like flavor (P < 0.05) with stronger flavors in grain than grass fed samples and liver-like flavor increasing with postmortem ageing time. Sour flavor was affected by diet only (P < 0.05) with grass fed samples having stronger sour flavor than grain fed samples. Ageing had an effect on overall tenderness (P < 0.05); samples aged 35 d were least tender, and samples aged 45 d were more tender than 55 d samples but did not differ from 65 d samples.ConclusionThe results suggest that beef flavor as measured by beef flavor ID and umami were not impacted by extended ageing; however, some off-flavors grew stronger as ageing time extended. Flavor attributes such as rancid, grassy, sour were stronger in grass than grain fed samples, but grain fed has a stronger liver-like flavor. Ageing influenced both overall tenderness and juiciness, but typically not in a linear fashion.

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
L. M. Wang ◽  
B. M. Bohrer

ObjectivesLimited research has investigated the effects of plant-based additives fed to feedlot cattle beyond cattle growth performance and carcass characteristics. Thus, the objective of this study was to investigate the effects of feeding antibiotic supplements versus essential oils and/or benzoic acid to finishing cattle on meat quality and sensory attributes of the longissimus thoracis (LT) muscle.Materials and MethodsCrossbred steers (N = 63) were placed into 3 blocks based on initial weight. Within each block, 1 of 5 treatments were randomly applied using an Insentec feeding system for 98 d: (1) control (CON) diet (no supplement); (2) monensin/tylosin (M/T) diet (monensin supplemented at 33 mg/kg on dry matter (DM) basis; tylosin supplemented at 11 mg/kg on DM basis); (3) essential oils (EO) diet (supplemented at 1.0 g/steer/day); (4) benzoic acid (BA) diet (supplemented at 0.5% on DM basis); and (5) combination (COMBO) diet (essential oils supplemented at 1.0 g/steer/day and benzoic acid supplemented at 0.5% on DM basis). Beef rib (IMPS#107) sections from the right side of carcasses were collected from a commercial processing facility and transported to the U of Guelph meat science laboratory and processed into 2.54 cm LT steaks. pH and objective color were collected for the LT steaks at 6 d post-mortem. Samples for cooking loss and shear force were aged for 7 d and 14 d post-mortem. Samples for sensory were aged for 7 d post-mortem. Duplicate 5 to 6 g homogeneously blended LT samples were analyzed for moisture content by forced-air convection oven drying at 100°C for 24 h (Method 950.46, AOAC. Lipid content of the dried samples were determined by Soxhlet extraction with petroleum ether, followed by 24 h of oven drying at 100°C. Cooking loss was measured after cooking samples to an internal temperature of 72°C. Warner-Bratzler shear force (WBSF) was measured on 1.3 cm diameter cores that were cut parallel to muscle fibers. Meat quality results were analyzed as a randomized complete block design (RCBD) with fixed effects of treatment, block and their interaction using PROC GLIMMIX of SAS. For sensory analysis, 8 highly trained panelists evaluated the tenderness, juiciness, chewiness, beef flavor intensity, and off-flavor intensity of steaks using a 15-cm line scale. Each steak was cooked to 68°C and served to each panelist as two 1-cm cubes. Results were analyzed as a RCBD with the fixed effects of treatment, panelist, and their interaction and the random effect of session.ResultsThere were no significant differences (P > 0.07) among treatments in this study for pH, objective color, % moisture, WBSF, or cooking loss of LT samples. Ribeye from the CON diet had significantly less % crude fat (P = 0.05) compared to other treatments. There was an effect of diet on the tenderness, chewiness, juiciness and beef flavor intensity of steaks as determined by the panelist. Specifically, CON and COMBO steaks were tougher, chewier and less juicy. All steaks had strong beef flavor, especially the BA steaks. Off-flavors were barely detectable.ConclusionResults showed that EO and BA when fed to finishing cattle do not affect meat quality. Trained panelists reported steaks in the M/T, EO, and BA diet were tender, juicier, and had stronger beef flavors. Potential off-flavors and off-aromas in finishing feed did not translate to beef products.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
D. Evers ◽  
A. J. Garmyn ◽  
J. F. Legako ◽  
D. R. Woerner ◽  
M. F. Miller

ObjectivesThe objective of this study was to evaluate the effects of extended wet-aging on the beef flavor profile of grass and grain-fed Australian strip loins.Materials and MethodsStrip loins (HAM 2140) were collected from grass and grain finished cattle (n = 50) at a commercial abattoir near Brisbane, Australia. Subprimals were portioned into sections and assigned randomly to 1 of 3 postmortem aging periods (45, 70, or 135 d). Portions were individually vacuum packaged and shipped refrigerated (0–4°C) to Texas Tech University in Lubbock, TX. Upon arrival, the strip loin sections were sorted into respective aging groups of 45D, 70D, and 135D and stored at 1–2°C. On each respective day, sections were fabricated into 2.54-cm steaks, vacuum packaged and frozen (–21°C). Electric clamshell grills were used to cook thawed (held at 2–4°C for 24 h) steaks to a medium degree of doneness (71°C); cooked temperatures were recorded. Steaks were cut into cubes and evaluated by trained panelists (n = 6) for descriptive sensory attributes using a 100-mm anchored line scales (0 = slight, 50 = moderate and 100 = strong).ResultsThe sour flavor was the only trait where an interaction between diet and postmortem aging was detected (P < 0.01). Samples aged 135 d from both grass and grain were similarly (P > 0.05) scored with a stronger (P < 0.05) sour flavor than all other treatment combinations, which did not differ (P > 0.05). Aging impacted (P ≤ 0.01) beef flavor ID, liver-like, metallic, rancid, green-hay, umami, and bitter flavors, as well as overall juiciness and overall tenderness. For beef flavor ID, 45D aging resulted in the greatest intensity (P < 0.05), while 70D samples were intermediate, and 135D samples were the least intense. For liver-like, metallic, rancid, green-hay, and bitter flavors, 135D samples had the strongest flavor, while 70D samples were intermediate, and 45D samples had the weakest flavor intensity (P < 0.05). For umami, 45D samples had stronger (P < 0.05) umami flavor than 135D samples, but 70D samples did not differ from either 45D or 135D (P > 0.05). Panelists rated 70D and 135D samples juicier (P < 0.01) than 45D samples, but 70D and 135D did not differ (P > 0.05). For overall tenderness, panelists rated 135D samples more tender (P < 0.05) than 45D and 70D, which were similar (P > 0.05). Diet impacted (P < 0.05) bloody/serumy, liver-like, green-hay, and bitter flavors. For bloody/serumy and liver-like, the grain fed treatments resulted in greater (P < 0.05) flavor intensity than grass fed treatments. However, grass fed samples had stronger (P < 0.05) green-hay and bitter flavors compared to grain fed samples. Diet and aging had no effect (P > 0.05) on fat-like or sweet flavors.ConclusionExtending postmortem aging of Australian beef strip loins from 45 to 135 d resulted in decreased beef and umami flavors, along with concurrent increased detection of off-flavors, such as liver-like, rancid, and sour. Diet influenced fewer flavor traits than postmortem aging, but grass-fed samples still had stronger green-hay flavor, as would be expected. Based on these results, aging beef strip loins 135 d is not recommended based on reduced beef flavor and increased off-flavor detection.


2012 ◽  
Vol 69 (11) ◽  
pp. 1881-1893 ◽  
Author(s):  
Verena M. Trenkel ◽  
Mark V. Bravington ◽  
Pascal Lorance

Catch curves are widely used to estimate total mortality for exploited marine populations. The usual population dynamics model assumes constant recruitment across years and constant total mortality. We extend this to include annual recruitment and annual total mortality. Recruitment is treated as an uncorrelated random effect, while total mortality is modelled by a random walk. Data requirements are minimal as only proportions-at-age and total catches are needed. We obtain the effective sample size for aggregated proportion-at-age data based on fitting Dirichlet-multinomial distributions to the raw sampling data. Parameter estimation is carried out by approximate likelihood. We use simulations to study parameter estimability and estimation bias of four model versions, including models treating mortality as fixed effects and misspecified models. All model versions were, in general, estimable, though for certain parameter values or replicate runs they were not. Relative estimation bias of final year total mortalities and depletion rates were lower for the proposed random effects model compared with the fixed effects version for total mortality. The model is demonstrated for the case of blue ling (Molva dypterygia) to the west of the British Isles for the period 1988 to 2011.


2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 22-22
Author(s):  
Charles A Zumbaugh ◽  
Susannah A Gonia ◽  
Kathryn M Payne ◽  
Thomas B Wilson

Abstract The objectives of this experiment were to determine changes in the nutritive value and ergot alkaloid concentrations of endophyte-infected tall fescue hay and haylage during a 180-d storage period. Forage from a single field of Kentucky-31 tall fescue was cut for hay in late June and allowed to dry in the field. The dry matter (DM) of the windrow of cut forage was measured every 2 h after clipping. Forage was sampled from the windrow in 6 location blocks once forage DM reached target levels for haylage and hay treatments. Haylage and hay samples were taken when the DM of the windrow reached 50% and 80%, respectively. Seven subsamples of each treatment within block were chopped to 1.91 cm in length with a lettuce chopper and vacuum sealed in oxygen-excluding bags. Sample bags were stored indoors and opened at 30 d intervals over the 180-d storage period. Samples were analyzed for pH, nutritive value, and individual ergot alkaloid concentrations using high-performance liquid chromatography. Within each storage day, treatment within block was considered the experimental unit. Data were analyzed in SAS using the MIXED procedure with fixed effects of treatment, day, and the treatment by day interaction. Location block was considered a random effect. As expected, pH was decreased for haylage compared to hay at all time points (P &lt; 0.01) and DM was greater (P &lt; 0.01) for hay compared to haylage. Neutral detergent fiber values were greater (P &lt; 0.01) for hay compared to haylage and declined during storage (P &lt; 0.01). Total ergot alkaloid concentrations did not differ by treatment (P = 0.61), but ergovaline concentrations declined (P &lt; 0.01) during storage. Collectively, these results indicate minimal differences in nutritive value and ergot alkaloid concentrations between hay and haylage during storage, and that ergovaline concentrations decline during storage.


Author(s):  
Rachel J Sorensen ◽  
James S Drouillard ◽  
Teresa L Douthit ◽  
Qinghong Ran ◽  
Douglas G Marthaler ◽  
...  

Abstract The effect of hay type on the microbiome of the equine gastrointestinal tract is relatively unexplored. Our objective was to characterize the cecal and fecal microbiome of mature horses consuming alfalfa or Smooth Bromegrass (brome) hay. Six cecally cannulated horses were used in a split plot design run as a crossover in 2 periods. Whole plot treatment was ad libitum access to brome or alfalfa hay fed over two 21-d acclimation periods with subplots of sampling location (cecum and rectum) and sampling hour. Each acclimation period was followed by a 24-h collection period where cecal and fecal samples were collected every 3 h for analysis of pH and volatile fatty acids (VFA). Fecal and cecal samples were pooled and sent to a commercial lab (MR DNA, Shallowater, TX) for amplification of the V4 region of the 16S rRNA gene and sequenced using Illumina HiSeq. Main effects of hay on VFA, pH, and taxonomic abundances were analyzed using the MIXED procedure of SAS 9.4 with fixed effects of hay, hour, location, period, all possible interactions and random effect of horse. Alpha and β diversity were analyzed using the R Dame package. Horses fed alfalfa had greater fecal than cecal pH (P ≤ 0.05) whereas horses fed brome had greater cecal than fecal pH (P ≤ 0.05). Regardless of hay type, total volatile fatty acid (VFA) concentrations were greater (P ≤ 0.05) in the cecum than in feces, and alfalfa resulted in greater (P ≤ 0.05) VFA concentrations than brome in both sampling locations. Alpha diversity was greater (P ≤ 0.05) in fecal compared to cecal samples. Microbial community structure within each sampling location and hay type differed from one another (P ≤ 0.05). Bacteroidetes were greater (P ≤ 0.05) in the cecum compared to the rectum, regardless of hay type. Firmicutes and Firmicutes:Bacteroidetes were greater (P ≤ 0.05) in the feces compared to cecal samples of alfalfa-fed horses. In all, fermentation parameters and bacterial abundances were impacted by hay type and sampling location in the hindgut.


2021 ◽  
pp. 227797522096830
Author(s):  
Palaniappan Gurusamy

The study aims to examine the relationship between corporate ownership structure and capital structure of BSE listed manufacturing firms in India. The study has included the sample of 357 companies which covers 16 major sectors during the period of 2006–2015. Considering the dynamic panel nature of the data relating to the capital structure and the ownership structure variables. The analysis undertakes a novel approach of examining the determinants both single equation and reduced equation models. In order to determine the most appropriate model, based on the F test, the Breusch Pagan LM test and finally the Hausman Test is conducted. The Hausman test result has been estimated by the fixed effect model is better than the other two models such as pooled OLS and random effect estimation. Based on the fixed effects results, size, risk and profitability have a highly significant relationship with leverage. Meanwhile, the growth opportunities and tangibility represent insignificant values. The study found that the explanatory variables of the promoters’ ownership and the institutional ownership have a negative impact on leverage, while the corporate ownership has a positive influence on the capital structure decision. The individual or public ownership has a negative and significantly related to the capital structure, whereas the effect of the foreign ownership inversely related to the firm’s leverage.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P &lt; 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P &lt; 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.


Author(s):  
Katherine D Vande Pol ◽  
Andres F Tolosa ◽  
Caleb M Shull ◽  
Catherine B Brown ◽  
Stephan A S Alencar ◽  
...  

Abstract Piglets experience a decline in body temperature immediately after birth, and both drying and warming piglets at birth reduces this. However, these interventions may have less effective at higher farrowing room temperatures. This study was carried out at a commercial facility to compare the effect of drying and/or warming piglets at birth on postnatal rectal temperature (RT) under relatively warm farrowing room temperatures (26.6 ± 2.09°C). Forty-five sows/litters were used in a completely randomized design to compare three Intervention Treatments (applied at birth): Control (no treatment); Warming (piglets placed in a plastic box under a heat lamp for 30 min); Drying+Warming (piglets dried with desiccant and warmed as above). Temperatures in the warming boxes over the study period averaged 37.7 ± 2.75°C. At birth, piglets were weighed; RT temperature was measured at 0, 10, 20, 30, 45, 60, 120, and 1440 min after birth. Blood samples were collected at 24 h after birth from a subsample of one piglet from each birth weight quartile within each litter to measure plasma immunocrit concentration. Data were analyzed using PROC MIXED of SAS with litter as the experimental unit; and piglet a subsample of litter. The model for analysis of piglet rectal temperature included fixed effects of treatment, measurement time (repeated measure), the interaction, and the random effect of sow. Compared to the Control, piglet RT were higher (P ≤ 0.05) for the Warming treatment between 10 and 60 min, and higher (P ≤ 0.05) for the Drying+Warming treatment between 10 and 120 min after birth. Rectal temperatures were higher (P ≤ 0.05) for the Drying+Warming than the Warming treatment between 20 and 120 min. Responses to drying and/or warming were greater for low birth weight piglets (&lt; 1.0 kg) than heavier littermates, but were generally less than observed in previous experiments with similar treatments carried out under cooler temperatures. Piglet immunocrit values were lower (P ≤ 0.05) for the Drying+Warming treatment compared to the other treatments, which were similar (P &gt; 0.05). Immunocrit values tended (P = 0.10) to be lower for light (&lt; 1.0 kg) compared to heavier birth weight piglets. In conclusion, drying and warming piglets at birth was more effective for reducing piglet RT decline after birth than warming alone, though the effect was less than observed in previous studies carried out under cooler farrowing room temperatures.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 221-222
Author(s):  
Melanie D Trenhaile-Grannemann ◽  
Ronald M Lewis ◽  
Stephen D Kachman ◽  
Kenneth J Stalder ◽  
Benny E Mote

Abstract Conformation-based sow selection is performed prior to reaching mature size, yet little is known about how conformation changes as growth continues. To assess conformation changes, 9 conformational traits were objectively measured at 12 discrete time points between 112 d of age and parity 3 weaning on 622 sows in 5 cohorts. The 9 traits included 5 body size traits (body length, body depth at the shoulder and flank, and height at the shoulder and flank) and 4 joint angles (knee, hock, and front and rear pastern). Data were analyzed with a repeated measures model (SAS V 9.4) including cohort and time point as fixed effects, sire as a random effect, and heterogeneous compound symmetry as the covariance structure. Sire variance ranged from 0.16 (body depth shoulder) to 2.00 (body length) cm2 for body size traits and 2.28 (rear pastern) to 4.22 (front pastern) degrees2 for joint angles. Cohort had an effect on all traits (P &lt; 0.05). All traits displayed changes over time (P &lt; 0.001). Size traits increased between 112 d of age and parity 3 weaning (64.16 vs. 107.57 cm, 26.62 vs. 44.14 cm, 23.32 vs. 36.92 cm, 46.10 vs. 73.55 cm, 49.36 vs. 77.47 cm for body length, body depth shoulder and flank, and height shoulder and flank, respectively); however, they fluctuated within parity by increasing during gestation and decreasing at weaning. Knee angle decreased (164.12 vs. 150.72 degrees) while fluctuating within parity by decreasing in the second half of gestation and increasing after weaning. Front and rear pastern angles decreased over time (60.89 vs. 53.74 degrees and 64.64 vs. 55.50 degrees for front and rear pastern, respectively), while biologically negligible change was observed in hock angle (148.63 vs. 147.48 degrees). Sow conformation changes throughout life, and these changes may require consideration when making selection decisions.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 70-71
Author(s):  
Julia P Holen ◽  
Robert D Goodband ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Joel M DeRouchey

Abstract Two experiments were conducted to determine the effects of increasing levels of soybean meal (SBM) replacing feed grade amino acids in corn or corn-dried distillers grains with solubles (DDGS)-based diets on growth performance of late finishing pigs. In both experiments, there were 22 to 27 pigs per pen and 14 pens per treatment. Average length of the experiments was 35 (Exp. 1) and 29 days (Exp. 2). Diets were balanced to contain 0.70% SID Lys and 2,667 or 2,610 kcal NE/kg for Exp. 1 and 2, respectively. Minimum amino acid ratios relative to Lys were: Ile, 55; Met&Cys, 60; Thr, 65; Trp, 19.5, and Val, 70. Dietary crude protein ranged from 10.1 to 15.2 for Exp. 1 and 13.6 to 19.4 for Exp. 2. The statistical model considered fixed effects of treatment, linear and quadratic contrasts, and random effect of block. In Exp.1, 1,793 pigs (L337×1050, PIC; initially 104.9 ± 1.4 kg) were fed corn-based diets and pens of pigs were assigned to 1 of 5 dietary treatments with increasing SBM from 5 to 20%. Overall, average daily gain (ADG) and gain-to-feed (G:F) increased (linear; P &lt; 0.05) as SBM increased with the greatest improvement observed as SBM increased from 5 to 8.75%, with little improvement thereafter. In Exp. 2, 1,827 pigs (L337×1050, PIC; initially 97.9 ± 1.1 kg) were used in a similar study as Exp. 1, but all diets contained 25% DDGS and SBM levels increased from 0 to 16%. Overall, G:F and final bodyweight of pigs marginally improved (linear and quadratic, respectively; P &lt; 0.10) as SBM increased, with the greatest performance observed when diets contained 8% SBM. These results suggest that increasing SBM up to 8% at the expense of feed grade amino acids in corn or corn-DDGS-based diets improved ADG or G:F in late-finishing pigs.


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