scholarly journals Bio-degradation of Feathers Waste by the Staphylococcus epidermidis P3A

2020 ◽  
Vol 14 (4) ◽  
pp. 2611-2617
Author(s):  
A.R. Jagtap ◽  
M.G. Bodhankar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biochemical Analysis ◽  
Bacterial Strains ◽  
Feather Degradation ◽  
Environment Friendly ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Keratinous Waste ◽  
Potential Use ◽  
Selection Of

Development of industrialization is directly proportional to the development of nations. Rising industrializations also increase the pollutions. One of them is poultry industry which discharges a huge amount of keratinous waste. The present study focused on the selection of feather degrading bacteria from Barshi region of Maharashtra. Bacterial strains were grown in whole feather broth medium. Out of 16 proteolytic bacterial isolates, 9 feather degrading isolates were selected from the Parande road side feather waste dumping area. Among these isolates, P3A was selected and classified on the basis of morphological and biochemical analysis. 16S rRNA studies confirmed that the isolated strain was Staphylococcus epidermidis. Staphylococcus epidermidis P3A shows 84% feather degradation and 72 U/ml maximum keratinase activity after 96 hrs. The isolate shows potential use in feather waste disposal methods which are safe and environment friendly.

scholarly journals Screening and Characterisation of Keratin-Degrading Bacillus sp. from Feather Waste

2019 ◽  
pp. 1-12
Author(s):  
M. T. Dada ◽  
S. M. Wakil
Keyword(s):  
Bacillus Subtilis ◽  
Proteolytic Activity ◽  
Bacillus Licheniformis ◽  
Basal Medium ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Feather Degradation ◽  
Feather Waste ◽  
Degrading Bacteria ◽  
Significant Difference

Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, nitrogen, sulfur and energy. Feather degrading bacteria were screened for both proteolytic activity and keratin degradation on skimmed milk agar and keratin azure medium respectively. They were also screened for their ability to degrade other keratin substrates such as hair and nail. Results: Three of the isolates with higher feather degradation levels also showed high proteolytic activity and release of azure dye. They were selected and identified phenotypically and genotypically using 16S rRNA sequencing as Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. The bacteria were capable of degrading other keratin-containing substrates such as nail and hair. Bacillus subtilis-K50 and Bacillus licheniformis-K51 showed significant difference (P) in degradation among the three different keratin sources used yielding higher degradation with feather as keratin source with respective optical densities of 0.07 and 0.11 followed by hair and least in nails with optical densities of 0.05 and 0.07 respectively. Highest degradation of all the three keratin substrates was observed in Bacillus licheniformis-K51. Conclusion: The three isolated bacteria possess the ability to degrade keratin and utilize feather as keratin substrate. As a result, these can be considered as potential candidates for degradation and utilization of feather keratin.

scholarly journals Oral Bacteria as Potential Probiotics for the Pharyngeal Mucosa

2010 ◽  
Vol 76 (12) ◽  
pp. 3948-3958 ◽  
Author(s):  
Simone Guglielmetti ◽  
Valentina Taverniti ◽  
Mario Minuzzo ◽  
Stefania Arioli ◽  
Milda Stuknyte ◽  
...  
Keyword(s):  
Oral Bacteria ◽  
Upper Respiratory Tract ◽  
Bacterial Strains ◽  
Pharyngeal Mucosa ◽  
Probiotic Potential ◽  
Healthy Donors ◽  
Tract Infections ◽  
Potential Use ◽  
Selection Of

ABSTRACT The research described here was aimed at the selection of oral bacteria that displayed properties compatible with their potential use as probiotics for the pharyngeal mucosa. We included in the study 56 bacteria newly isolated from the pharynges of healthy donors, which were identified at the intraspecies level and characterized in vitro for their probiotic potential. The experiments led us to select two potential probiotic bacterial strains (Streptococcus salivarius RS1 and ST3) and to compare them with the prototype oral probiotic S. salivarius strain K12. All three strains efficiently bound to FaDu human epithelial pharyngeal cells and thereby antagonized Streptococcus pyogenes adhesion and growth. All were sensitive to a variety of antibiotics routinely used for the control of upper respiratory tract infections. Immunological in vitro testing on a FaDu layer revealed different responses to RS1, ST3, and K12. RS1 and ST3 modulated NF-κB activation and biased proinflammatory cytokines at baseline and after interleukin-1β (IL-1β) induction. In conclusion, we suggest that the selected commensal streptococci represent potential pharyngeal probiotic candidates. They could display a good degree of adaptation to the host and possess potential immunomodulatory and anti-inflammatory properties.

scholarly journals Antibacterial activity of selected snake venoms on pathogenic bacterial strains

2019 ◽  
Vol 27 (3) ◽  
pp. 305-317 ◽  
Author(s):  
Francisc Andrei Boda ◽  
Anca Mare ◽  
Zoltán István Szabó ◽  
Lavinia Berta ◽  
Augustin Curticapean ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Snake Venoms ◽  
Starting Point ◽  
Sds Page ◽  
Potential Use ◽  
Selection Of

Abstract Snake venoms are aqueous solutions containing peptides and proteins with various biochemical, physiological, and pathophysiological effects. Several snake venom components are used as lead molecules in the development of new active substances for the treatment of cardiovascular diseases, clotting disorders, cancer or pain. Antibacterial activity has also been attributed to snake venoms and proteins isolated from snake venoms. This study provides information regarding the antibacterial activity of venoms obtained from various snake species from the Elapidae and Viperidae families. Minimum inhibitory and bactericidal concentrations of snake venoms were determined for three Gram-positive (Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 29213, and Methicillin-resistant Staphylococcus aureus ATCC 43300) and three Gram-negative (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, and Pseudomonas aeruginosa ATCC 27853) pathogenic bacteria. The observed effects were correlated with the protein content of each venom, determined using SDS-PAGE analysis and comparison with data available in the literature. Our findings represent a starting point for the selection of snake venoms containing components with potential use as lead molecules in the development of new antibacterial agents, targeting multidrug resistant bacterial strains.

scholarly journals Bio-Mercury Remediation Suitability Index: A Novel Proposal That Compiles the PGPR Features of Bacterial Strains and Its Potential Use in Phytoremediation

2021 ◽  
Vol 18 (8) ◽  
pp. 4213
Author(s):  
Marina Robas ◽  
Pedro A. Jiménez ◽  
Daniel González ◽  
Agustín Probanza
Keyword(s):  
Phosphate Solubilization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Soil Bioremediation ◽  
Bacterial Strains ◽  
Auxin Production ◽  
Suitability Index ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Potential Use ◽  
Selection Of

Soil pollution from heavy metals, especially mercury, is an environmental problem for human health. Biological approaches offer interesting tools, which necessarily involve the selection of organisms capable of transforming the environment via bioremediation. To evaluate the potential use of microorganisms in phytorhizoremediation, bacterial strains were isolated from rhizospheric and bulk soil under conditions of chronic natural mercury, which were identified and characterized by studying the following: (i) their plant growth promoting rhizobacteria (PGPR) activities; and (ii) their maximum bactericide concentration of mercury. Information regarding auxin production, phosphate solubilization, siderophore synthesis and 1-aminocyclopropane-1-carboxylic acid deaminase (ACCd) capacity of the isolates was compiled in order to select the strains that fit potential biotechnological use. To achieve this objective, the present work proposes the Bio-Mercury Remediation Suitability Index (BMR-SI), which reflects the integral behavior of the strains for heavy metal polluted soil bioremediation. Only those strains that rigorously fulfilled all of the established criteria were selected for further assays.

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

scholarly journals Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

2013 ◽  
Vol 33 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Cintia Anabela Mazzucotelli ◽  
Alejandra Graciela Ponce ◽  
Catalina Elena Kotlar ◽  
María del Rosario Moreira
Keyword(s):  
Bacterial Strains ◽  
Isolation And Characterization ◽  
By Products ◽  
Potential Use

Wound-Care Resources on the Internet

2006 ◽  
Vol 96 (3) ◽  
pp. 264-268
Author(s):  
Charles R. Fikar ◽  
Brigitte Delinois
Keyword(s):  
Web Sites ◽  
Wound Care ◽  
Medical Practitioner ◽  
Antibiotic Use ◽  
Educational Process ◽  
Reference Source ◽  
Internet Resources ◽  
Hyperbaric Medicine ◽  
Potential Use ◽  
Selection Of

An updated selection of high-quality Internet resources related to wound and ulcer care is presented. Of potential use to the podiatric medical practitioner, educator, resident, and student, some Web sites that cover hyperbaric medicine, antibiotic use, and wound and ulcer prevention are also included. These Web sites have been evaluated on the basis of their potential to enhance the practice of podiatric medicine, in addition to contributing to the educational process. Readers who require a quick reference source to wound and ulcer care may find this report useful. (J Am Podiatr Med Assoc 96(3): 264–268, 2006)

scholarly journals SELECTION OF HERBICIDES TARGETING THE USE IN CROP SYSTEMS CULTIVATED WITH SHOWY CROTALARIA

2015 ◽  
Vol 33 (3) ◽  
pp. 521-534 ◽  
Author(s):  
G. B. P BRAZ ◽  
R. S OLIVEIRA JR ◽  
J. CONSTANTIN ◽  
H. K. TAKANO ◽  
C. A. CHASE ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Chemical Control ◽  
Clayey Soil ◽  
Chlorimuron Ethyl ◽  
High Production ◽  
Low Levels ◽  
Postemergence Herbicides ◽  
Potential Use ◽  
Selection Of

ABSTRACT The increase in the area planted with Crotalaria spectabilishas occurred by several factors, highlighting the potential to reduce the nematodes, nitrogen fixation and the high production of biomass. By becoming a species sown as a crop, it is necessary to control the weeds that coexist with showy crotalaria. This change in the use of this crop creates the possibility of this specie becoming a weed. The aim of this study was to assess the potential use of herbicides applied in preemergence and postemergence of C.spectabilisfor different purposes (control of volunteer and selectivity plants). Three experiments were installed in a greenhouse (two with herbicides applied in preemergence - in soils with distinct textural categories; and one experiment with herbicides applied in postemergence). The results of the experiments with herbicides applied in preemergence showed that: amicarbazone, atrazine, diuron, metribuzin, prometryn, fomesafen and sulfentrazone showed effectiveness for control of C.spectabilis in clayey soil. Besides these, flumioxazin and isoxaflutole also showed potential to be used in the control of showy crotalaria in soils with loam texture. In relation to the postemergence herbicides, atrazine, diuron, prometryn, flumioxazin, fomesafen, lactofen, saflufenacil, amonio-glufosinate and glyphosate can be used aiming the chemical control of C.spectabilis. Herbicides chlorimuron-ethyl, diclosulan, imazethapyr, pyrithiobac-sodium, trifloxysulfuron-sodium, clomazone, pendimethalin, S-metolachlor and trifluralin applied in preemergence, and imazethapyr, pyrithiobac-sodium, flumiclorac, bentazon and clethodim applied in postemergence caused low levels of injury to C.spectabilis plants, making necessary the development of new searches to ensure the selectivity of these products.

scholarly journals Use of multi-locus sequencing typing as identification method for the food-borne pathogen Listeria monocytogenes: a review

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Sonia Lamon ◽  
Domenico Meloni ◽  
Simonetta Gianna Consolati ◽  
Anna Mureddu ◽  
Rina Mazzette
Keyword(s):  
Listeria Monocytogenes ◽  
Detection Methods ◽  
Causative Organism ◽  
Intracellular Pathogen ◽  
Bacterial Strains ◽  
New Approach ◽  
Food Borne ◽  
Global Epidemiology ◽  
Selection Of

<em>Listeria monocytogenes</em> is an ubiquitous, intracellular pathogen which has been implicated within the past decade as the causative organism in several outbreaks of foodborne diseases. In this review, a new approach to molecular typing primarily designed for global epidemiology has been described: multi-<em>locus</em> sequencing typing (MLST). This approach is novel, in that it uses data that allow the unambiguous characterization of bacterial strains via the Internet. Our aim is to present the currently available selection of references on <em>L. monocytogenes</em> MLST detection methods and to discuss its use as <em>gold</em> <em>standard</em> to <em>L. monocytogenes</em> subtyping method.

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