scholarly journals Formulation of shea butter Nanoparticle containing griseofulvin: a combination of antifungal and anti-inflammatory treatments

2021 ◽  
Vol 11 (2-S) ◽  
pp. 59-65
Author(s):  
Sidy Mouhamed Dieng ◽  
Madieye Séne ◽  
Ahmedou Bamba Koueimel Fall ◽  
Moussa Diop ◽  
Papa Mady Sy ◽  
...  
Keyword(s):  
A Priori ◽  
Lipid Nanoparticles ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Shea Butter ◽  
Inversion Method ◽  
Temperature Phase ◽  
Anti Inflammatory ◽  
The Stability ◽  
Phase Inversion Method

Nanomedicine has been a booming industry with the development of nanovectors to encapsulate water-soluble or amphiphilic molecules for drug delivery. As the new therapeutic agents synthesized are increasingly lipophilic, the development of new nanoparticulate vectors allowing their transport and targeting is now a major challenge. These particles are lipid nanoparticles, a few hundred nanometers in diameter, stabilized by a layer of surfactants composed of castor oil and stealth agents. Solid lipid nanoparticles based on shea butter, stabilized by cremophor® ELP, encapsulating griseofulvin, were formulated by the temperature phase inversion method. The shea butter nanoparticles thus obtained were the subject of characterization relating to: determination of the morphology, size, polydispersity index, pH and zeta potential. The results confirm the stability of our preparations. The anti-inflammatory activity of shea butter being known, the tests were carried out on mice. The inflammation was induced by a solution of croton oil acetone. There is a very big improvement in anti-inflammatory activity. This is due to better penetration of the preparation through the different layers of the skin. Griseofulvin release studies have been carried out on our various preparations. Systems designed as reservoirs of active ingredients and intended for a priori controlled release obey kinetics of the order of one-half (½) corresponding to a proportionality between the quantity released and the square root of time. Shea butter in nanoparticulate forms has thus enabled us to considerably prolong the release of griseofulvin. Keywords: Nanoparticles; Shea Butter; Inflammation; Griseofulvin.

scholarly journals Study of the stability of lipid nanoemulsions with oleic acid

2021 ◽  
Vol 12 (2-2021) ◽  
pp. 174-176
Author(s):  
E. V. Mishchenko ◽  
◽  
E. E. Timofeeva ◽  
A. S. Artamonov ◽  
M. Yu. Koroleva ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Phase Inversion ◽  
Nonionic Surfactants ◽  
Inversion Method ◽  
Ionic Surfactant ◽  
Temperature Phase ◽  
The Stability ◽  
Tween 60 ◽  
Span 60 ◽  
Phase Inversion Method

This work presents the results of a study on the effect of ionic surfactant cetriltrimethylammonium chloride (CTAB) on the size and ζ-potential of lipid nanoemulsions composed of oleic acid, prepared by temperature phase inversion method and stabilized by nonionic surfactants — Tween 60, Span 60

scholarly journals ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

2017 ◽  
Vol 10 (6) ◽  
pp. 160
Author(s):  
Vinueza D ◽  
LÓpez E ◽  
Acosta K ◽  
Abdo S
Keyword(s):  
Freeze Drying ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Hydroalcoholic Extract ◽  
Cytotoxicity Assays ◽  
Aerial Parts ◽  
Controlled Conditions ◽  
Freeze Dried ◽  
Anti Inflammatory

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatoryactivity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference. 

scholarly journals STANDARDIZATION AND STABILITY EVALUATION OF DRY EXTRACTS OF MYRACRODRUON URUNDEUVA ALLEMÃO OBTAINED BY SPRAY DRIER

2017 ◽  
Vol 9 (2) ◽  
pp. 154 ◽  
Author(s):  
Renata Da Silva Leite ◽  
Valmir Gomes De Souza ◽  
Agna HÉlia De Oliveira ◽  
JosÉ VenÂncio Chaves JÚnior ◽  
Islaine De Souza Salvador ◽  
...  
Keyword(s):  
Silicon Dioxide ◽  
Feed Rate ◽  
Stability Study ◽  
Inflammatory Activity ◽  
Inlet Temperature ◽  
Dry Extract ◽  
Anti Inflammatory ◽  
The Stability ◽  
Drying Conditions ◽  
Quercetin Concentration

<p><strong>Objective</strong>:<strong> </strong>This study aimed to obtain standardised dry extracts of <em>Miracrodruon urundeuva </em>Allemão using spray-dryer and evaluate the stability of the extracts.</p><p><strong>Methods</strong>:<strong> </strong>It evaluated the drying parameters: Proportion of colloidal silicon dioxide (CSD) (10, 15 and 20%), inlet temperature (160, 170 and 180 °C) and feed rate (4, 6 and 8 ml/min). The study of the accelerated stability of dry extract occurred in temperature of 40 °C (±2 °C) and relative humidity of 75% (±5%) for 6 mo. The anti-inflammatory activity of the dry extract was evaluated in Swiss mice by the paw edema method.</p><p><strong>Results</strong>:<strong> </strong>Variations in drying conditions did not represent significant variations in yields of the process. The drying temperature and feed rate significantly influenced the concentration of quercetin (p≤0.05). The increase in inlet temperature and feed flow promoted the increase of quercetin concentration in the extracts. The stability study showed that the concentration of quercetin in dry extract was stable over a period of 6 mo. The dry extract showed anti-inflammatory activity in mice orally.</p><p><strong>Conclusion</strong>:<strong> </strong>A condition of 10% of colloidal silicon dioxide with an 180 °C inlet temperature and a feed rate of 8 ml/min was considered the most adequate for obtaining the extracts and the drying process resulted in stable dry extracts and the quercetin was a suitable biomarker for monitoring the process.</p>

scholarly journals Anti-Inflammatory Activity of Water-Soluble Polysaccharide ofAgaricus blazeiMurill on Ovariectomized Osteopenic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Peng Wang ◽  
Xiao-Tao Li ◽  
Lei Sun ◽  
Lei Shen
Keyword(s):  
Interleukin 1 ◽  
Total Antioxidant Status ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovx Rats ◽  
Total Antioxidant ◽  
Soluble Polysaccharide ◽  
Cox 2 ◽  
Anti Inflammatory

In the present study, we investigated the anti-inflammatory activity of water-soluble polysaccharide ofAgaricus blazeiMurill (WSP-AbM) on ovariectomized osteopenic rats. The rats were administered orally WSP-AbM (200 mg/kg BW) for 8 weeks. Subsequent serum maleic dialdehyde (MDA) level, total antioxidant status (TAOS), nuclear factor kappa B (NF-κB) level, polymorphonuclear (PMN) cells level, interleukin-1β(IL-1β) level, inducible nitric oxide synthase (iNOS) level, tumor necrosis factor-α(TNF-α) level, adhesion molecule (ICAM-1), and cyclooxygenase-2 (COX-2) were determined by enzyme linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. WSP-AbM administration markedly (P<0.05) decreased serum IL-1βand TNF-αlevels and the expressions of ICAM-1, COX-2, and iNOS NF-κB compared with OVX rats. WSP-AbM administration alsomarkedly (P<0.05) decreased PMN infiltration. In conclusion, we observed that WSP-AbM supplementation had anti-inflammatory effects in a model of osteoporosis disease.

scholarly journals Formulation Design and Preclinical Evaluations of Surface Modified Lipid Nanoparticles-Coupled Gel Encapsulating Dihydroartemisinin for Treatment of Localized Inflammation

2021 ◽  
Vol 11 (3) ◽  
pp. 3745-3769
Keyword(s):  
Particle Size ◽  
Sustained Release ◽  
Surface Charge ◽  
Ex Vivo ◽  
Lipid Nanoparticles ◽  
Inflammatory Activity ◽  
Drug Content ◽  
Ex Vivo Permeation ◽  
Anti Inflammatory ◽  
Surface Modified

Previously, it has been claimed that artemisinin derivatives, e.g., dihydroartemisinin, possess very potent anti-inflammatory activity. The study aimed to formulate gels based on surface-modified nanostructured lipid carrier (NLC) and contain dihydroartemisinin (DHA) to treat localized inflammation. NLC was developed using Softisan®154 and Tetracarpidium conophorum oil and structured using PEG 4000. Physicochemical characterization of NLC, including surface charge, particle size, and encapsulation efficiency (EE%), was evaluated. NLCwas dispersed in hydroxypropyl cellulose, and the resulting nanogels were evaluated for drug content, ex vivo permeation, and anti-inflammatory activity. The surface charge and particle size of NLC ranged from -15.3 ± 1.1 to -25.5 ± 2.1 mV and 85.5 ± 8.6 – 108.7 ± 5.5 nm respectively. EE% of NLC was in the range of 90.0 ± 1.21 – 99.3 ± 1.60 %. NLC gels had high drug content (83 – 99 %). Ex vivo permeation study showed sustained-release of DHA over 24 h. The gels produced a sustained-release reduction of egg albumin-induced inflammation in rats up to 8 h for 7 days. Development of surface-modified lipid nanoparticles-based gel containing DHA produced controlled release of the drug localized inflammation.

scholarly journals Lipid Nanoparticles for Enhancing the Physicochemical Stability and Topical Skin Delivery of Orobol

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 845
Author(s):  
Min-Hwan Kim ◽  
Yae-Eun Jeon ◽  
Soobeen Kang ◽  
Jae-Young Lee ◽  
Ki Won Lee ◽  
...  
Keyword(s):  
Skin Irritation ◽  
Entrapment Efficiency ◽  
Aging Effect ◽  
Lipid Nanoparticles ◽  
Skin Aging ◽  
Shea Butter ◽  
Skin Delivery ◽  
Cadaver Skin ◽  
The Stability

Orobol is one of the major soy isoflavones, and has been reported to have various pharmacological activities, including an anti-skin-aging effect. However, since it has low solubility in water and physicochemical instability, the formulation of orobol for delivery into the dermal layer of the skin could be challenging. The objective of this study was to prepare lipid nanoparticles formulations of orobol to enhance its stability as well as its deposition into the skin. Formulations of orobol-loaded solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) were characterized in terms of their mean particle size, entrapment efficiency, and morphology. The nano-sized spherical NLCs formulations maintained the stability of orobol for up to 28 days. Moreover, the NLCs formulation significantly increased the in vitro deposition of orobol into both Strat-M membranes and human cadaver skin compared with the other formulations. Additionally, the NLCs formulation did not cause significant skin irritation in clinical study. These results demonstrate that a shea butter-based NLC formulation could be a promising and safe carrier system for improving the stability of orobol and enhancing its topical skin delivery.

LIPID NANOPARTICLES FOR TRANSDERMAL DELIVERY OF CELECOXIB: AN IN VITRO AND IN VIVO INVESTIGATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 38-48
Author(s):  
S. V Shinde ◽  
S Nikam ◽  
P Raut ◽  
M. K. Ghag ◽  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Ex Vivo ◽  
Research Work ◽  
Lipid Nanoparticles ◽  
Inflammatory Activity ◽  
Promising Alternative ◽  
Solid Lipid ◽  
Anti Inflammatory

In the present research work, celecoxib (CXB) loaded solid lipid nanoparticles (SLNs) were prepared using the probe sonication method, wherein Glyceryl monostearate and Tween 80 were used as solid lipid and surfactant, respectively. To obtain the statistically optimized batch, 32 factorial design was applied. The optimized batch was characterized physicochemically and evaluated through DSC, SEM and XRD studies. The mean particle size of the optimized batch was found to be 135.41± 0.24 nm with a mean % entrapment efficiency of 80 ± 1.69%. The optimized batch was further lyophilized and dispersed into 1% w/v Carbopol 934P to form a gel. Prepared gel was further evaluated for in vitro drug release, occlusivity, ex vivo permeability, local toxicity, in vivo anti-inflammatory activity and accelerated stability study. The study resulted in stable, safe and prolonged anti-inflammatory activity with quick onset of action. Hence, celecoxib loaded solid lipid nanoparticles can be considered as promising alternative to conventional topical systems.

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