scholarly journals Studies on Biosynthesis of Silver Nanoparticles Using Rhizopus sp. and its Antibacterial Efficacy on E. coli MDR Strains

2014 ◽  
Vol 23 ◽  
pp. 27-35
Author(s):  
Jyothi Hiremath ◽  
Vandana Rathod ◽  
Shivaraj Ninganagouda ◽  
Dattu Singh ◽  
K. Prema
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Human Life ◽  
Antibacterial Properties ◽  
Pharmaceutical Products ◽  
Diffusion Method ◽  
Plasmon Resonance Peak ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Vis Spectroscopy

Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.

scholarly journals Ecofriendly synthesis of silver nanoparticles from leaves extract of Phyllanthus niruri (L.) and their antibacterial properties

2019 ◽  
Vol 9 (1-s) ◽  
pp. 196-200
Author(s):  
G Amalorpavamary ◽  
G Dineshkumar ◽  
K Jayaseelan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Good Alternative ◽  
Diffusion Method ◽  
Surface Plasmon Resonance Peak ◽  
Phyllanthus Niruri ◽  
Plasmon Resonance Peak ◽  
Synthesis Of Nanoparticles ◽  
Average Size

In recent times, plant-mediated synthesis of nanoparticles has garnered wide interest owing to its inherent features such as rapidity, simplicity, eco-friendliness and cheaper costs. For the first time, silver nanoparticles were successfully synthesized using Phyllanthus niruri leaf extract in the current investigation. The silver nanoparticles were characterized by UV–Vis spectrophotometer and the characteristic surface plasmon resonance peak was identified to be 423 nm. The morphology of the silver nanoparticles was characterized by scanning electron microscopy (SEM). The size of the silver nanoparticles was found to be 10-50 nm, with an average size 15 nm.  FTIR analysis was done to identify the functional groups responsible for the synthesis of the AgNPs. The antibacterial potential of synthesized AgNPs was compared with that of aqueous extracts of P.niruri by well diffusion method. The AgNPs at 50µl concentration significantly inhibited bacterial growth against A.hydrophila (16 ± 0.09 mm). Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future. Keywords: Phyllanthus niruri, AgNps, Aeromonas hydrophila, Antibacterial Activity.

scholarly journals GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY

2017 ◽  
Vol 10 (3) ◽  
pp. 196 ◽  
Author(s):  
S C Joshi ◽  
Utkarsh Kaushik ◽  
Aproova Upadhyaya ◽  
Priyanka Sharma
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Atomic Force Microscope ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Scanning Electron

ABSTRACTObjective: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. Silver nanoparticlesare usually synthesized by chemicals and physical method, which are quite toxic and flammable in nature. This study deals with an environmentfriendly biosynthesis process of antibacterial silver nanoparticles using Momordica charantia fruit.Methods: AgNO3 (5 mM) was allowed to react with fruit extract of M. charantia. Biosynthesis of AgNPs was optimized by changing temperature,pH, and solvent. The silver nanoparticles so formed were characterized using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infraredspectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscope (AFM), and scanning electron microscopy (SEM).Results: UV-VIS spectra show absorption peak between 420 and 430 nm. The FTIR analysis showed the alcoholic, lactam, and nitro group presentin the plant extract, which were responsible for the reduction in AgNPs. The SEM images showed the size distribution of the nanoparticles and theaverage size was found to be 50-100 nm. By DLS analysis and AFM analysis, average sizes of the silver nanoparticles were of 150 nm. The results ofthese analyses confirmed the formation of silver nanoparticles. Silver nanoparticles were tested against Bacillus cereus and Staphylococcus epidermidisstrains using disc diffusion method and were found to be effective.Conclusion: Silver nanoparticles so synthesized in this study using fruit extract of M. charantia are simple, easy, and effective technique of nanoparticlesproduction.Keywords: Silver nanoparticles, Momordica charantia, Optimization, Antibacterial, Atomic force microscope, Scanning electron microscopy.

scholarly journals Green Synthesis and Antibacterial Activity of HAp@Ag Nanocomposite Using Centella asiatica (L.) Urban Extract and Eggshell

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Xuan Nui Pham ◽  
Hoa Thi Nguyen ◽  
Ngan Thi Pham
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Antibacterial Properties ◽  
Centella Asiatica ◽  
Diffusion Method ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Green Approach

In recent years, the green synthesis of nanoparticles via biological processes has attracted considerable attention. Herein, we introduce a facile and green approach for the synthesis of poriferous silver nanoparticles (Ag-NPs) decorated hydroxylapatite (HAp@Ag) nanoparticles with excellent antibacterial properties. All the nanocomposites were fully characterized in the solid state via various techniques such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectrometer (EDX), in which the synthesized Ag-NPs (24 nm in diameter) and their homogeneous incorporation on HAp have been studied by ultraviolet-visible (UV-vis) technique, transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. The obtained results indicate that the structure and morphology of HAp have no significant changes after the incorporation of Ag-NPs on its surface. Moreover, an impressive antibacterial activity of HAp@Ag nanocomposite against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa has been recorded by using the agar well diffusion method. As a result, the HAp@Ag nanocomposite promises to be a great biomedical material with high antibacterial properties.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

scholarly journals Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Francisco Javier Alvarez-Cirerol ◽  
Marco Antonio López-Torres ◽  
Ericka Rodríguez-León ◽  
César Rodríguez-Beas ◽  
Aaron Martínez-Higuera ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Early Mortality ◽  
White Shrimp ◽  
Ros Generation ◽  
Early Mortality Syndrome ◽  
Selected Area Electron Diffraction ◽  
Worldwide Production ◽  
Vis Spectroscopy

Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Syndrome (AHPNS) is a disease produced by gram-negative bacteria Vibrio parahaemolyticus (V. parahaemolyticus), which has caused declines in worldwide production of a white shrimp Litopenaeus vannamei (L. vannamei). In this work, we propose the implementation of silver nanoparticles (AgNPs) synthesized with Rumex hymenosepalus (Rh) extract as an alternative on V. parahaemolyticus control. AgNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED). AgNP mean sizes by DLS were 80.82±1.16 nm and sizes between 2 and 10 nm by TEM, with a zeta potential of −47.72±1.05 mV. This study evaluated AgNPs and Rh antimicrobial capacity on V. parahaemolyticus at different concentrations; the minimum inhibitory concentration (MIC) found was 25 μg/mL for AgNPs and 220 μg/mL for Rh. Additionally, were carried out time-kill curves and reactive oxygen species (ROS) generation for 1 and 4 MIC. Both concentrations (MIC) were tested for toxicity on Artemia nauplii from Artemia franciscana (A. franciscana), because nauplii were used as biocarriers for AgNPs and Rh extract on L. vannamei. Once the shrimp were treated, they were challenged with Vibrio infection and it was found that those who were treated with both agents showed greater survival than the control. V. parahaemolyticus and postlarval samples were taken from the bioassay and fixed and prepared for TEM and SEM in order to search NPs in internal structure of bacteria and the hepatopancreatic area of shrimps; AgNPs were detected in both cases. AgNPs and Rh extract show antibacterial properties on the infected shrimp with V. parahaemolyticus. The action mechanisms are interaction with the bacterial membrane and ROS generation; these effects are produced by both agents.

scholarly journals BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

2016 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Eli Rohaeti ◽  
Endang W Laksono ◽  
Anna Rakhmawati
Keyword(s):  
Waste Water ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Nitrate Solution ◽  
Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy ◽  
Chitosan Composite

<pre><span lang="EN-GB">Bacterial cellulose (C) and its composites were synthesized from rice waste water<strong> </strong>with addition of glycerol (G) and chitosan (Ch).</span><strong></strong><span lang="EN-GB">Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against <em>S. aureus</em>, <em>E.</em> <em>coli</em>,  and yeast <em>C. albicans</em> has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against <em>S</em>. <em>aureus</em>, <em>E. coli</em>, and <em>C. albicans</em> by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of <em>S. aureus</em>, <em>E. coli</em>,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the <em>Staphylococcus aureus</em> ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the <em>E. coli</em> ATCC 25922 and the yeast <em>Candida albicans</em> ATCC 10231.</span></pre>

scholarly journals Silver Nanoparticles Obtained by Semicontinuous Chemical Reduction Using Carboxymethyl Cellulose as a Stabilizing Agent and Its Antibacterial Capacity

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
M. A. Pedroza-Toscano ◽  
S. López-Cuenca ◽  
M. Rabelero-Velasco ◽  
E. D. Moreno-Medrano ◽  
A. P. Mendizabal-Ruiz ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Carboxymethyl Cellulose ◽  
Chemical Reduction ◽  
Weight Ratio ◽  
Visible Spectrum ◽  
Reducing Agent ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Vis Spectroscopy

Preparation of silver nanoparticles was carried out by semicontinuous reduction of Ag+ ions at low temperatures. Silver nitrate was used as the Ag0 precursor, the carboxymethyl cellulose (CMC) as stabilizer and primary reducing agent, and sodium borohydride as reducing agent. Weight ratios of 1 : 1 and 1 : 2 of AgNO3 : CMC were used for carrying out the reactions. Silver nanoparticles were characterized by UV-VIS spectroscopy, transmission electronic microscopy (TEM), and X-ray diffraction (XRD). The formation of silver nanoparticles was confirmed by XRD spectroscopy and by the presence of an absorption peak around 400 nm in the UV-visible spectrum. Unimodal size distributions of spheroidal nanoparticles were observed by TEM. Greater productivities than those reported by other authors were obtained with the advantage of using a lower temperature and minor reaction times. By using a higher CMC/AgNO3 weight ratio or a higher concentration of AgNO3, AgNPs with larger average size were produced. Antibacterial activity of AgNPs against S. aureus and E. coli was determined by the agar disk diffusion method. The higher the AgNPs concentration, the larger the inhibition zone. The minimum inhibitory concentration (MIC) of AgNPs against S. aureus and E. coli was 5 μg/disk.

Investigating the role of phytochemicals in silver nanoparticle as an antibacterial agent using computational and experimental analysis

2021 ◽  
Vol 16 (12) ◽  
pp. 100-108
Author(s):  
Akanksha Dubey ◽  
Jayanthi Sivaraman
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Binding Proteins ◽  
Chemical Constituents ◽  
Experimental Studies ◽  
Antibacterial Properties ◽  
Bacterial Strains ◽  
Penicillin Binding Proteins ◽  
Synthesis Of Nanoparticles

Designing a powerful approach for the synthesis of metal nanoparticles is a critical footstep in the field of nanotechnology. Algae-mediated synthesis of nanoparticles is a substitute to overthrow the restrictions of traditional methods. Penicillin-binding proteins are proteins binding to β-lactams and are convoluted in cell wall biosynthesis. The present study aimed to investigate the potential role of phytochemicals in inhibiting these penicillin binding proteins against bacterial agents using computational and experimental studies. Biosynthesis of silver nanoparticles was done using aqueous extract of Dictyota bartayresiana and was evaluated for antibacterial activity. Characterization was done via UV-visible spectroscopy, Scanning electron microscopy, Transmission electron microscopy and Xray diffraction studies. It was found that synthesized nanoparticle was spherical in shape and possessed antibacterial property against Staphylococcus aureus and Escherichia coli. Phytochemical screening was performed to identify the chemical constituents present in silver nanoparticles followed by molecular docking studies against penicillin binding proteins found in bacterial strains. In silico designing of silver nanoparticles was done using material science suite followed by probe target interactions. The results displayed a highly stable binding amongst designed nanoparticle and phytochemicals and indicated that the silver nanoparticles possessed antibacterial properties due to phytochemicals present in the extract.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM FRUIT RIND EXTRACT OF GARCINIA MANGOSTANA L. AND EVALUATION OF ANTIBACTERIAL PROPERTIES

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Manju Madhavan ◽  
Elsa Rose P.J. ◽  
Arunima Francis ◽  
Grace Maria Benny ◽  
Anila Wilson
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Wave Length ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Toxic Substances ◽  
Garcinia Mangostana ◽  
Vis Spectroscopy ◽  
Fruit Rind

Nanotechnology is an emerging area of science that involves the engineering of nano sized particles of various materials. Among the various nanoparticles, the silver nanoparticles are used in various applications due to their unique characters. The production of silver nanoparticles using a chemical method is harmful and produces toxic substances as by-products, so the aim of our study was to green synthesis silver nanoparticles using the fruit rind of Garcinia mangostana L. which is being discarded as a waste material. Aqueous rind extract of G. mangostana was prepared and synthesis of silver nanoparticles was analysed by UV-Vis spectrophotometer by optimizing various parameters like pH, time and concentration of extract. The nanoparticles were characterized using UV-Vis spectroscopy and the peak was obtained between the wave length of 410 - 433 nm in various treatments. The antibacterial activities of synthesized silver nanoparticles were tested against both gram negative (Pseudomonas) and gram positive (Staphylococcus) bacteria using the well diffusion method. The aqueous extract shows the remarkable zone of inhibition against Pseudomonas and Staphylococcus.

scholarly journals Investigating the Possibility of Green Synthesis of Silver Nanoparticles Using Vaccinium arctostaphlyos Extract and Evaluating Its Antibacterial Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Sedighe Khodadadi ◽  
Nafiseh Mahdinezhad ◽  
Bahman Fazeli-Nasab ◽  
Mohammad Javad Heidari ◽  
Baratali Fakheri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Ftir Spectroscopy ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antibacterial Properties ◽  
Silver Nitrate Solution ◽  
X Ray Diffraction ◽  
X Ray ◽  
Vis Spectroscopy

Objective. Vaccinium genus plants have medicinal value, of which Vaccinium arctostaphylos (Caucasian whortleberry or Qare-Qat in the local language) is the only available species in Iran. Public tendency to use herbal remedies and natural products such as synthesized nanoparticles is increasing due to the proof of the destructive side effects of chemical drugs. Nanosilver products have been effective against more than 650 microbe types. This study was aimed at assessing the possibility of green synthesis of silver nanoparticles using Vaccinium arctostaphylos aqueous extract and at evaluating its antibacterial properties, as well. Materials and Methods. In order to synthesize silver nanoparticles, different volumes of Vaccinium arctostaphylos aqueous extract (3, 5, 10, 15, and 30 ml) were assessed with different silver nitrate solution concentrations (0.5, 1, 3, 5, and 10 mM) and different reaction time durations (1, 3, 5, 10, and 20 minutes) at room temperature using a rotary shaker with a speed of 150 rpm. Ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction analysis (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) were carried out. The antibacterial activity of the aqueous extract and the synthesized nanoparticles was evaluated, as well. Results. Silver nanoparticle formation process was confirmed with XRD analysis, transmission electron microscopy (TEM), and FTIR spectroscopy. The UV-Vis spectroscopy of silver colloidal nanoparticles showed a surface plasmon resonance peak at 443 nm under optimal conditions (3 ml aqueous extract volume, 1 mM silver nitrate solution concentration, and 3 min reaction time under sunlight exposure). The reduction of silver ions to silver nanoparticles in solution was confirmed, as well. Based on X-ray diffraction analysis, the size of silver nanoparticles was in the range of 7-16 nm. TEM images showed an even distribution of silver nanoparticles, with a spherical shape. FTIR spectroscopy demonstrated the presence of different functional groups of oxygenated compounds such as carboxyl, hydroxyl, and nitrogenous groups. The antibacterial properties of the synthesized nanoparticles were confirmed. Conclusion. The synthesized nanoparticles showed more antibacterial properties against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) than gram-negative ones (Escherichia coli and Salmonella enteritidis).

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