scholarly journals Efektivitas Stabilitas Bacillus thuringiensis H-14 Isolat Salatiga Sediaan Bubuk Terhadap Jentik Anopheles SPP. di Kabupaten Purworejo

2020 ◽  
Vol 48 (3) ◽  
Author(s):  
Arief Nugroho ◽  
Arum Triyas Wardani ◽  
Rendro Wianto

Abstract Malaria is a vector-borne disease that is still a health problem in Indonesia. Control of vector mosquito larvae using Bacillus thuringiensis (Bt H-14) is an alternative biolarvicide. B2P2VRP has developed Salatiga Bt H-14 isolate in powder preparation which need to be tested for effectiveness stability. This study aims to determine the effectiveness stability of Salatiga powder Bt H-14 isolate against larvae of Anopheles spp. Preparation of Salatiga powder Bt H-14 isolate and its bioassay test against larvae of Anopheles aconitus were carried out at laboratory of the B2P2VRP. The stability test was carried out at the laboratory of UII Yogyakarta. The stability effectiveness test was carried out in the field in Purworejo using Anopheles spp. larvae taken in the field. The bioassay test (LC90 ) at initial and after stored at 54 o C ± 2 o C for two weeks were 6.485 ppm and 13.009 ppm, respectively. The study showed the effectiveness of stability decreased on days 2 and 3 (<70%) at dose of 26,018 ppm because of rain. Statistical test showed a difference in the effectiveness of reducing larvae mortality at days 2 and 3. The study confirmed the reduction of effectiveness of Salatiga powder Bt H-14 Isolate after stability test. Keywords: Anopheles larvae, Bacillus thuringiensis, effectiveness, Purworejo, stability Abstrak Malaria merupakan salah satu penyakit tular vektor yang masih menjadi masalah kesehatan di Indonesia. Pengendalian jentik nyamuk vektor menggunakan Bacillus thuringiensis (Bt H-14) merupakan salah satu biolarvisida alternatif. B2P2VRP telah mengembangkan Bt H-14 isolat Salatiga sediaan bubuk maka perlu diuji stabilitas. Penelitian ini bertujuan menentukan efektivitas stabilitas Bt H-14 isolat Salatiga sediaan bubuk terhadap jentik Anopheles spp di lapangan. Pembuatan Bt H-14 Isolat Salatiga sediaan bubuk dan uji bioassay terhadap jentik Anopheles aconitus dilakukan di laboratorium B2P2VRP. Uji stabilitas dilakukan di laboratorium UII Yogyakarta. Uji efektivitas stabilitas dilakukan di Purworejo menggunakan jentik Anopheles spp. yang diambil di lapangan. Hasil uji bioassay (LC90 ) sebelum dan setelah penyimpanan suhu 54 o C ± 2 o C selama 2 minggu adalah 6,485 ppm dan 13,009 ppm. Hasil menunjukkan efektivitas stabilitas menurun pada hari ke 2 dan 3 (<70%) pada dosis 26,018 ppm adanya pengaruh hujan. Uji statistik menunjukkan adanya perbedaan efektivitas penurunan kematian jentik saat hari ke 2 dan 3. Hasil penelitian menunjukkan adanya penurunan efektivitas dari Bt H-14 Isolat Salatiga sediaan bubuk setelah dilakukan uji stabilitas. Kata kunci : Anopheles larvae, Bacillus thuringiensis, efektivitas, Purworejo, stabilitas

Author(s):  
Yulidar Yulidar

One of vector borne deseases which still remains a health problem in Indonesia is dengue fever (DBD). This desease is caused by dengue virus transmitted by Aedes aegypti mosquitoes. One of cities which is endemic for DBD in 2014 was Banda Aceh. The indicator for DBD endemic is based on entomology index. One of entomology index to be measured is the number of mosquito larvae-free. To know the status of mosquito larvae-free number in Banda Aceh, a suvey has been conducted in three subdistricts within Banda Aceh city, namely Banda Raya (Lamlagang village), Ulee Kareng (Ceurih village), and Baiturrahman (Peuniti village). A survey was conducted in 100 houses of every subdistrict. The total of 300 houses were investigated. From 300 houses investigated, there were 158 houses were positively had mosquito larvae. From 860 containers that were found and checked in those three subdistricts, it was found that there were 235 containers were positively contained mosquito larvae. The container which contained mosquito larvae dominantly was bathups inside the houses, there were 48,22% of larvae and 60,47% of pupas. The results showed that the number of mosquito larvae-free for Banda aceh was 47,33% which indicated that it was still below the number of national larvae-free that is > 95%. Therefore, Banda Aceh is still in a high risk of DBD transmission in the future.


Author(s):  
Junhao Huang ◽  
Fang Fang ◽  
Zhihua Wang ◽  
Mingxuan Hang ◽  
Yibo Wang ◽  
...  

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Szymon Tomczak ◽  
Aleksandra Gostyńska ◽  
Malwina Nadolna ◽  
Karolina Reisner ◽  
Marta Orlando ◽  
...  

Intravenous drug incompatibilities are a common cause of medical errors, contributing to ineffective therapy and even life-threatening events. The co-administration of drugs must always be supported by studies confirming compatibility and thus guarantee the therapy’s safety. Particular attention should be paid to the possible incompatibilities or degradation of intravenous cephalosporins in different infusion regimens since the administration of drugs with inadequate quality may cause treatment failure. Therefore, an appropriate stability test should be performed. The study aimed to present various aspects of the stability and compatibility of five cephalosporins: cefepime (CFE), cefuroxime (CFU), ceftriaxone (CFX), ceftazidime (CFZ), and cefazoline (CFL). The degradation studies in parenteral infusion fluids and PN admixtures were conducted for CFE and CFU. The interactions between CFX or CFZ and PN admixtures, as well as the compatibility of CFL with five commercial parenteral nutrition (PN) admixtures, were investigated. The content of CFX and CFZ in PN admixture after 24 h was >90%. CFL administered simultaneously with PN admixture by the same infusion set using Y-site was compatible only with Nutriflex Lipid Special. CFE and CFU were stable in all tested infusion fluids for a minimum of 48 h and decomposed in PN admixtures during storage.


Author(s):  
Mevi Irianti Tonapa ◽  
Rani Dewi Pratiwi ◽  
Elsye Gunawan

Kenop Flower (Gomphrena globosa L.) is used in the manufacture of lip cream because contains betacyanin pigments that function as color pigments. This study aims to determine the physical quality and stability of the lip cream preparation of the ethanol extract of kenop flower (Gomphrena globosa L.). This research was conducted experimentally, including the manufacture of lip cream formulations with ethanol extract of kenop flower (Gomphrena globosa L.) with a concentration of 10%. The results of the physical examination test for lip cream preparations for all preparations have a distinctive vanilla aroma with a semi-solid texture, F0 has ivory white color and F1-F3 has a brown color. The preparations had a homogeneous composition, average pH 6-7, had good greasing power, 5.0-5.8 average dispersion and 60.33-66.67 seconds average adhesion. The stability test carried out on day 28 found that all preparations were stable, had a distinctive vanilla aroma with a semi-solid texture, F0 had ivory white color and F1-F3 had a brown color. The preparation has a homogeneous composition; the average pH is 6-7. Where the lip cream formulas F1 and F3 decreased the pH value on the 28th day from 7 to 6 but tended to be stable and in the pH range that matched the lip pH. And there is no phase separation in all formulas.


2013 ◽  
Vol 63 (2) ◽  
Author(s):  
Nur Syahidah Yusoff ◽  
Maman Abdurachman Djauhari

The stability of covariance matrix is a major issue in multivariate analysis. As can be seen in the literature, the most popular and widely used tests are Box M-test and Jennrich J-test introduced by Box in 1949 and Jennrich in 1970, respectively. These tests involve determinant of sample covariance matrix as multivariate dispersion measure. Since it is only a scalar representation of a complex structure, it cannot represent the whole structure. On the other hand, they are quite cumbersome to compute when the data sets are of high dimension since they do not only involve the computation of determinant of covariance matrix but also the inversion of a matrix. This motivates us to propose a new statistical test which is computationally more efficient and, if it is used simultaneously with M-test or J-test, we will have a better understanding about the stability of covariance structure. An example will be presented to illustrate its advantage


2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Glaucia A. Rocha-Selmi ◽  
Carmen S. Favaro-Trindade ◽  
Carlos R. F. Grosso

The interest in lycopene has increased in recent years due to studies that associate it with the reduction in risk of developing cardiovascular diseases and cancer. However, due to its high degree of unsaturation, this carotenoid is inclined to isomerize and oxidize during processing and storage, making it difficult to use in the food industry. Microencapsulation can improve this situation, increasing its stability and making incorporation into food formulations possible. Thus, the aim of this study was to microencapsulate lycopene by complex coacervation using gelatin and gum Arabic as the encapsulating agents. The microcapsules were evaluated based on the encapsulation efficiency and their morphology and then submitted to a stability test and applied in cake making. Most of the systems studied presented spherical microcapsules with defined walls. The encapsulation efficiency values were above 90%, and the average diameter of the capsules ranged from 61 to 144 μm. The stability test showed that microencapsulation offered greater protection to the lycopene as compared to its free form. The application of nonfreeze dried coacervated microcapsules in cake making was satisfactory, but the color transference was low when freezedried coacervated microcapsules were used.


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