Identification of Secondary Metabolite Compounds and Cytotoxic Activity Test of Papuan Grape (Sararanga Sinuosa Hemsley) Extract using Brine Shrimp Lethality Test (BSLT) Method

Papua has a diversity of flora species, one of which is the Papuan Grape (Sararanga sinuosa Hemsley). It is commonly used by the Depapre community, Jayapura, as a stamina booster. This research aims to identify the secondary metabolite compounds, to test the cytotoxic activity of Papuan Grape (Sararanga sinuosa Hemsley) extract, and to determine the best concentration that inhabits the growth of Artemia salina larvae using the BSLT method. This study was conducted with the extraction stage using the maceration method by making use of 96% ethanol solvent. Subsequently, the concentration series 0, 50, 100, 150, 200, 250, 300 ppm of Papuan Grape (Sararanga sinuosa Hemsley) extract were made to test the cytotoxic activity on the mortality of Artemia salina shrimp larvae. The results showed that Alkaloids, Flavonoids, Saponins, and Tannins were compounded as secondary metabolite. An antioxidant research that had been carried out previously had LC50 of green-white fruit (12,49 ± 0,35 mg/ml), orange-red fruit (17,62 ± 3,49 mg/ml) and red fruit (12,23 ± 0,46 mg/ml). The community process one stalk of it into juice and used or consumed it two times a day. An inappropriate dose of traditional medicine usage can affect the organ system and had adverse effects in the future The result of cytotoxic research obtained the value of LC50 in ethanol extract of Papuan Grape was 140,863 ppm, and concentration of 250 ppm was the best concentration to inhibit the growth of shrimp larvae (Artemia salina L). The conclusion of this study was the ethanol extract of Papuan Grape (Sararanga sinuosa Hemsley) showed the highest cytotoxic activity and potentially become an anti-cancer agent.

Formulation and Stability Test of Lip Cream Preparation of Kenop Flower Ethanol Extract (Gomphrena globosa L.)

Kenop Flower (Gomphrena globosa L.) is used in the manufacture of lip cream because contains betacyanin pigments that function as color pigments. This study aims to determine the physical quality and stability of the lip cream preparation of the ethanol extract of kenop flower (Gomphrena globosa L.). This research was conducted experimentally, including the manufacture of lip cream formulations with ethanol extract of kenop flower (Gomphrena globosa L.) with a concentration of 10%. The results of the physical examination test for lip cream preparations for all preparations have a distinctive vanilla aroma with a semi-solid texture, F0 has ivory white color and F1-F3 has a brown color. The preparations had a homogeneous composition, average pH 6-7, had good greasing power, 5.0-5.8 average dispersion and 60.33-66.67 seconds average adhesion. The stability test carried out on day 28 found that all preparations were stable, had a distinctive vanilla aroma with a semi-solid texture, F0 had ivory white color and F1-F3 had a brown color. The preparation has a homogeneous composition; the average pH is 6-7. Where the lip cream formulas F1 and F3 decreased the pH value on the 28th day from 7 to 6 but tended to be stable and in the pH range that matched the lip pH. And there is no phase separation in all formulas.

Systematic Literature Review: The Effectiveness of giving Cinnamon (Cinnamon) on Reducing the Intensity of Menstrual Pain (Dysmenorrhea)

Every woman experiences menstruation, which is the process of releasing blood, mucus, and cell debris from the dissertation of the uterine mucosa by removing the endometrium (desquamation) periodically and cyclically. It begins about 14 days after ovulation. Sometimes menstruation causes pain (primary dysmenorrhea) during menstruation. Primary dysmenorrhea can be reduced with natural ingredients, one of which is cinnamon, which has the main compounds cinnamaldehyde and eugenol as anti-inflammatory and antispasmodic. This study aimed to determine the effectiveness of cinnamon in reducing the intensity of menstrual pain (dysmenorrhea). The method used Systematic Literature Review or SLR to analyze articles from some databases The results showed that eight from ten studies were researched with the intervention group (cinnamon) and two journals were descriptive studies. Eight cinnamon intervention groups, three used pills, two used aromatherapy massage, one used cinnamon tea, one used cinnamon extract, and one did not explain the method. All journals show that cinnamon is effective for reducing the intensity of dysmenorrhea.

Antioxidant Activity of Dendrobium lasianthera and Geodorum densiflorum

Many antioxidants are obtained from plants that contain secondary metabolic compounds such as alkaloids, flavonoids, saponins, tannins, etc. Thus, the search for plants that are rich in natural antioxidants is conducted continuously, including orchids. Dendrobium lasianthera and Geodorum densiflorum are orchids having secondary metabolites that are thought to have antioxidant activity. This study aims to determine the antioxidant activity of the ethanolic extracts of these two types of orchids using the DPPH method (1,1-diphenyl-2-picrylhydrazine). The results showed that the IC50 of the ethanolic extract of the leaves of D. lasianthera was 288.24 ppm and the stem was 26.27 ppm. The IC50 of the ethanol extract of leaves of G. densiflorum was 277.65 ppm and of the tubers was 197.48 ppm. Finally, the leaves have negligible antioxidant activity, but the stems and tubers had activity ranging from weak to very strong.

Antibacterial Activity Test of Various Fraction of Lick leaves (Villebrunea rubescens) against Staphylococcus aureus and Staphylococcus epidermidis Bacterial

Lick leaves (Villebrunea rubescens) are one of the medicinal plants from the Urticaceae family. In Papua, we called it Daun jilat, which is used empirically by the community to treat contusion or bruises in the Serui area, Yapen Island Regency, Papua, Indonesia. Based on the result of the phytochemical screening, V. rubescens contained Alkaloids, Tannins, Saponins, Quinones, and Flavonoids. In previous research on an extract of Lick leaves, it was found that the antibacterial activities within inhibition zone have value of 8.7 mm (125 ?g/mL). This research aims to find out the antibacterial activities of the various fraction of Lick leaves in inhibiting the growth of Staphylococcus epidermidis and Staphylococcus aureus bacterial and to find out the most effective concentration of the various fraction of Lick leaves. The method used in this research was disc diffusion method and the solution used was ethanol-water, ethyl acetate, and N-hexane with the variable concentration used was 100, 250, 500, 750, 1000ppm. The result showed that Lick leaves fraction of ethanol-water, ethyl acetate, and n-hexane had an inhibition zone against the growth of Staphylococcus epidermidis bacteria, while the n-hexane and ethyl acetate had an inhibition zone against the growth of Staphylococcus aureus bacteria. However, for the ethyl-acetate, the inhibition zone was categorized as weak. The concentration of Lick leaves which effectively inhibited the growth of bacteria was ethanol-water fraction with an inhibition zone of 7.78 mm against Staphylococcus epidermidis bacteria and ethanol-water fraction with an inhibition zone of 3.89 mm against Staphylococcus aureus bacteria.