scholarly journals Efek Protektif Sinergis Kombinasi Minyak Jintan Hitam (Nigella sativa) Komersial dan Madu terhadap Nefrotoksisitas pada Tikus akibat Cisplatin

2020 ◽  
Vol 9 (1) ◽  
pp. 67-80
Author(s):  
Rita Noviana ◽  
Muhammad In‟am Ilmiawan ◽  
Mitra Handini
Keyword(s):  
Combination Index ◽  
Nigella Sativa ◽  
Thiobarbituric Acid ◽  
Treated Group ◽  
Male Rats ◽  
Thiobarbituric Acid Reactive Substance ◽  
Dose Treatment ◽  
Treatment Groups ◽  
Nigella Sativa Oil ◽  
Ad Libitum

Abstrak Cisplatin (CP) merupakan agen kemoterapi yang banyak digunakan dan menyebabkan nefrotoksisitas. Minyak jintan hitam (MJH) dan madu (M) diketahui memiliki efek nefroprotektif. Tujuan: Mengetahui efek dan sinergitas kombinasi MJH dan madu pada kadar malondialdehid (MDA) ginjal tikus yang diberi pajanan CP. Metode: Penelitian ini menggunakan 30 ekor tikus jantan yang dibagi menjadi 10 kelompok: Kontrol Normal (KN) (diet ad libitum), MJH1 (1 mL/kgBB MJH), MJH2 (2 mL/kgBB MJH), M1 (3,7 mL/kgBB M), M2 (7,4 mL/kgBB M), MJH1-M1 (1 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH1-M2 (1 mL/kgBB MJH dan 7,4 mL/kgBB M), MJH2-M1 (2 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH2-M2 (2 mL/kgBB MJH dan 7,4 mL/kgBB M), dan CP (diet ad libitum). MJH dan madu diberikan secara oral selama 21 hari. Pada hari ke-18, KN diberikan NaCl 0,9% 1 ml/kgBB sedangkan sisanya diberikan CP dosis tunggal 8 mg/kgBB secara intraperitoneal. Kadar MDA ginjal diukur menggunakan metode thiobarbituric acid reactive substances (TBARS). Didapatkan kadar MDA ginjal yang lebih rendah pada semua kelompok dosis tunggal dan kombinasi dibandingkan dengan kelompok CP (p=0,000). Semua kelompok dosis kombinasi memiliki indeks kombinasi <1. Kombinasi MJH dan madu memberikan efek protektif sinergis terhadap kerusakan oksidatif jaringan ginjal tikus akibat pajanan CP. Kata kunci: Minyak jintan hitam, madu, indeks kombinasi, cisplatin, nefrotoksisitas Abstract Cisplatin (CP) is a widely used chemotherapeutic agent that induces nephrotoxicity. Nigella sativa oil (NSO) and honey (H) have demonsrated nephroprotective effect. To investigate the effect and synergy of NSO and honey combination on kidney malondialdehyde (MDA) levels in CP-induced rats. Thirty male rats were divided into ten groups: Normal Control (NC), NSO1, NSO2, H1, H2, NSO1-H1, NSO1-H2, NSO2-H1, NSO2-H2, and CP-treated group which received ad libitum diet, 1 mL/kgbwt. NSO, 2 mL/ kgbwt. NSO, 3.7 mL/kgbwt. H, 7.4 mL/kgbwt. H, 1 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 1 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, 2 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 2 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, ad libitum diet, respectively, for 21 days. On the 18th day, NC group received 1 ml/kgbwt. NaCl 0.9% while the rest received 8 mg/kgbwt. single dose CP intraperitoneally. Kidney MDA levels was measured using thiobarbituric acid reactive substance (TBARS) method. Kidney MDA levels was significantly lower in all single and combination dose treatment groups compared to CP-treated group (p=0,000). Combination index of all combination dose treatment groups is <1. NSO and honey combination provided synergistic protective effect against CP-induced oxidative damage in kidney tissue in rats. Keywords: Nigella sativa oil, honey, combination index, cisplatin, nephrotoxicity

scholarly journals Effect of different smoking methods on the quality of pork sausages

2018 ◽  
pp. 1712-1719 ◽  
Author(s):  
Debajit Bhuyan ◽  
Ankur Das ◽  
Saurabh Kumar Laskar ◽  
Durlav Prasad Bora ◽  
Shantanu Tamuli ◽  
...  
Keyword(s):  
Shear Force ◽  
Thiobarbituric Acid ◽  
Plate Count ◽  
Thiobarbituric Acid Reactive Substance ◽  
Refrigerated Storage ◽  
Treatment Groups ◽  
Acceptable Limit ◽  
Significant Difference ◽  
The Mean ◽  
Pork Sausages

Aim: An experiment was conducted to evaluate the nutritional, physicochemical, microbiological, and sensory attributes of pork sausages treated with conventional smoking (CS) and liquid smoke (LS). Materials and Methods: Pork sausages were prepared by employing CS (T1) and by addition of LS at 3% (T2A), 5% (T2B), and 7% (T2C) while smoking was not done in control (C) sausages. The ready-to-eat pork sausages were evaluated in terms of proximate composition, emulsion stability (ES), cooking loss (CL), pH, water activity (aw), texture profile analysis (TPA), and shear force on the day of preparation and the shelf life of the sausages was evaluated on the basis of thiobarbituric acid reactive substance (TBARS) value, organoleptic qualities, total viable plate count, total psychrophilic count, and yeast and mold counts at 5-day interval up to 15 days under refrigerated storage (6±1°C). Results: The mean percentage moisture and percentage ether extract contents of the conventionally smoked sausages (T1) exhibited significant difference (p≤0.01) with the rest of the formulations. However, in terms of mean percentage crude protein and percentage total solids, no significant difference (p≥0.05) was recorded between the treatment groups. The mean ES (ml of oil/100 g emulsion) of the different sausage emulsions ranged from 1.88 to 3.20, while the mean aw values among the sausage formulations were found to be non-significant. In terms of mean percentage, CL and pH values, significantly lowest (p≤0.01) values were recorded by the T1 sausages. The mean TBARS values recorded at different periods of time in respect of all the treatment groups ranged from 0.10 to 0.33 mg malanoldehyde [MDA]/kg of sausages which are well within the permissible limit. The highest shear force values (KgF) were recorded by the sausages of T1 formulation (p≤0.01), while TPA of the sausages did not record any significant difference (p≥0.05) among the treatments. Organoleptic studies revealed acceptability of the sausages up to 10 days of refrigerated storage irrespective of treatments employed; however, the sausages of T1 formulation scored significantly (p≤0.01) higher panel ratings. Microbiologically, sausages with different formulations were found to be within the acceptable limit up to the 15th day of refrigerated storage. Conclusion: The study revealed that traditional hot smoking has slightly higher edges over the LS-treated sausages in terms of lipid oxidation, microbiological safety, and sensory panel ratings. However, if not superior, the same was found to be well within the acceptable limit in case of LS-treated sausages proving the potentiality of the use of LS as a suitable replacement for the traditional hazardous hot smoking process.

scholarly journals Protective role of Nigella sativa oil on renal damage induced by acetaminophen in male rats

2017 ◽  
Vol 40 (2) ◽  
pp. 77-81
Author(s):  
Zena M. Hamad
Keyword(s):  
Serum Creatinine ◽  
Blood Urea Nitrogen ◽  
Nigella Sativa ◽  
Nitrogen Concentration ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Control Group ◽  
Urea Nitrogen ◽  
Nigella Sativa Oil ◽  
Nitrogen Concentrations

     Acetaminophen also called paracetamol is commonly used as analgesic and antipyretic agent which in high doses causes liver and kidney damage in man and animals. Nigella sativa oil have antioxidant properties. Thirty adult male rats were used and randomly divided into three equal groups. Group (A) untreated and served as control group; Group (B) rats were orally intubated (by gavages needle) acetaminophen suspension (150mg/kg B.W). Group (C) rats were given orally acetaminophen suspension (150mg/kg) plus 1ml/kg B.W of Nigella sativa oil for 42 days in both treated group. Fasting blood samples were collected at 21 and 42 days of experiment to study the following parameters:  Serum creatinine concentration and blood urea nitrogen concentration. The results revealed a significant increase of acetaminophen group in serum creatinine and blood urea nitrogen concentrations as compression with GA. Animals treated with Nigella sativa oil plus acetaminophen (C) showed a significant decline in serum creatinine and blood urea nitrogen concentrations. In conclusion, the acetaminophen was effective in induction of oxidative stress and change in some biological markers related to kidney disease. Also it seems that Nigella sativa oil exerts protective actions against the damaging effect of acetaminophen

scholarly journals HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

2017 ◽  
Vol 9 (10) ◽  
pp. 225
Author(s):  
Nadhira M. Dinar ◽  
Sheila Pratiwi ◽  
Rain Kihara ◽  
Nadia G. Paramita ◽  
Nazilla R. Fathurrahman ◽  
...  
Keyword(s):  
Body Weight ◽  
New Zealand ◽  
Oryctolagus Cuniculus ◽  
Nigella Sativa ◽  
Treated Group ◽  
Nigella Sativa Oil ◽  
Oral Group ◽  
Nephroprotective Activity ◽  
Per Oral ◽  
New Zealand Rabbits

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

scholarly journals Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract

2013 ◽  
Vol 3 (7) ◽  
pp. 563-568 ◽  
Author(s):  
Sherif W. Mansour ◽  
Sibghatullah Sangi ◽  
Sree Harsha ◽  
Mueen A. Khaleel ◽  
A.R.N. Ibrahim
Keyword(s):  
Olive Oil ◽  
Nigella Sativa ◽  
Male Rats ◽  
Nigella Sativa Oil

scholarly journals Development of nutritional iron deficiency in growing male rats: haematological parameters, iron bioavailability and oxidative defence

2010 ◽  
Vol 105 (4) ◽  
pp. 517-525 ◽  
Author(s):  
María J. M. Alférez ◽  
Javier Díaz-Castro ◽  
Inmaculada López-Aliaga ◽  
María Rodríguez-Ferrer ◽  
Luis Javier Pérez-Sánchez ◽  
...  
Keyword(s):  
Thiobarbituric Acid ◽  
Iron Bioavailability ◽  
Male Rats ◽  
Haematological Parameters ◽  
Fe Deficiency ◽  
Albino Rats ◽  
Thiobarbituric Acid Reactive Substance ◽  
Sequence Of Events ◽  
Wistar Albino Rats ◽  
The Brain

Despite Fe deficiency having been widely studied, the sequence of events in its development still remains unclear. The aim of the present study was to elucidate the effects of nutritional Fe-deficiency development on haematological parameters, Fe bioavailability and the enzymes involved in oxidative defence in recently weaned male Wistar albino rats. Control (C) and Fe-deficient (ID) groups were fed the AIN-93 G diet with a normal Fe level (45 mg/kg diet) or with a low Fe level (5 mg/kg diet), respectively, for 20, 30 or 40 d. At day 20 serum Fe, serum ferritin and the saturation of transferrin decreased drastically, decreasing further in the course of Fe-deficiency development for the saturation of transferrin. The development of Fe deficiency did not affect plasma thiobarbituric acid-reactive substance production, or catalase (CAT) and glutathione peroxidase (GPx) activities in erythrocyte cytosol. Fe deficiency diminished hepatic Fe content and CAT and GPx activities in hepatic cytosol only at day the 20. However, in spite of the minor Fe deposits in the brain of ID rats, the CAT and GPx activities in the brain cytosolic fraction did not differ in any of the studied periods v. control rats. These results show that brain is a tissue that does not seem to depend on Fe levels for the maintenance of antioxidant defence mechanisms in the course of nutritional Fe deficiency.

Combined administration of taurine and meso 2,3–dimercaptosuccinic acid in the treatment of chronic lead intoxication in rats

2004 ◽  
Vol 23 (4) ◽  
pp. 157-166 ◽  
Author(s):  
S JS Flora ◽  
Manisha Pande ◽  
Smrati Bhadauria ◽  
G M Kannan
Keyword(s):  
Oxidative Stress ◽  
Soft Tissues ◽  
Aminolevulinic Acid ◽  
Thiobarbituric Acid ◽  
Concomitant Administration ◽  
Significant Inhibition ◽  
Male Rats ◽  
Lead Intoxication ◽  
Zinc Protoporphyrin ◽  
Thiobarbituric Acid Reactive Substance

The present study describes the dose–dependent effect of taurine, an amino acid and a known antioxidant, either alone or in combination with meso 2,3–dimercaptosucci–nic acid (DMSA) in the treatment of subchronic lead intoxication in male rats. The effects of these treatments in influencing the lead–induced alterations in haem synthesis, hepatic, renal or brain oxidative stress and lead concentration from soft tissues were investigated. Exposure to lead produced a significant inhibition of blood–aminolevulinic acid dehydratase (ALAD) activity, reduction in glutathione (GSH) and an increase in zinc protoporphyrin (ZPP) suggesting an altered haem synthesis pathway. Only DMSA was able to increase the activity of ALAD, while both taurine and DMSA were able to significantly increase GSH level towards normal. Animals treated with taurine significantly reduced the alterations in some of the biochemical parameters indicative of oxidative stress. Thiobarbituric acid reactive substance (TBARS) levels reduced significantly in liver, kidney and red blood cells, while GSH level increased. Activity of superoxide dismutase (SOD) also showed an increase in blood and brain in animals treated with taurine. The data also provided a promising role of taurine during chelation of lead by potentiating the depletion of blood, liver and brain lead compared to DMSA alone. It can thus be concluded from the study that concomitant administration of an antioxidant could play a significant and important role in abating a number of toxic effects of lead when administered along with the thiol chelators.

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