PERBANDINGAN KADAR NITRIC OXIDE PADA PEROKOK DAN BUKAN PEROKOK

Abstract Background: Cigarette smoke can affect macrophage metabolism by activating macrophages to release leukotrien B4, IL-8 and TNFα which lead to increased production of superoxide (O2-) and H2O2 and also cause oxidative damage to macromolecules such as lipids, proteins, DNA that can eliminate antioxidants and form free radicals such as Nitric Oxide (NO). NO has an important contribution in the occurrence of infection where NO will be produced more by iNOS. Methods: This study is a cross sectional study with a sample of samples taken in total sampling from medical students of Jambi University as many as 22 people with smokers and 22 non-smokers. Conducted NO examination by using microplate reader on wave 595. Research data obtained then tested by statistical analysis with mann whitney and wilcoxon test. Results : NO levels in smokers were higher than nonsmoker group NO levels. Based on normality test data with Mann Whitney obtained significant difference between both groups value of sig 0.030. Furthermore, Wilcoxon analysis. The result of wilcoxon analysis shows statistic test output where sig (<0,05). Conclusion : NO levels in the smoker group were higher than in the nonsmokers group. NO levels between smokers and nonsmokers were significantly different. Keywords : Smokers, non-smokers, nitric oxide Abstrak Pendahuluan : Asap rokok dapat mempengaruhi metabolisme makrofag dengan mengaktifkan makrofag untuk melepaskan leukotrien B4, IL-8 dan TNFα yang menyebabkan meningkatnya produksi superoksida (O2-) dan H2O2 dan juga menyebabkan kerusakan oksidatif makromolekul seperti lipid, protein, DNA yang dapat menghilangkan antioksidan serta membentuk radikal bebas seperti Nitrit Oxide (NO). NO mempunyai kontribusi yang penting dalam terjadinya infeksi dimana NO akan diproduksi lebih banyak oleh iNOS. Metode : Penelitian ini merupakan penelitian cross sectional dengan sampel penelitian yang diambil secara total sampling dari mahasiswa kedokteran Universitas Jambi sebanyak 22 orang dengan perokok dan 22 orang bukan perokok. Dilakukan pemeriksaan NO dengan menggunakan microplate reader pada gelombang 595. Data penelitian yang di dapatkan kemudian di uji dengan analisis statistic dengan uji mann whitney dan wilcoxon. Hasil : Kadar NO pada perokok lebih tinggi dibandingkan dengan kadar NO kelompok bukan perokok. Berdasarkan uji normalitas data dengan Mann whitney diperoleh perbedaan yang bermakna diantara kedua kelompok nilai sig 0.030. Selanjutnya dilakukan Analisis Wilcoxon. Hasil analisis wilcoxon menunjukkan output test statistic dimana sig (<0,05). Kesimpulan : Kadar NO pada kelompok perokok lebih tinggi dibandingkan dengan kelompok bukan perokok. Kadar NO antara kelompok perokok dan bukan perokok terdapat perbedaan yang signifikan. Kata Kunci : Perokok, bukan perokok, Nitric Oxide

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The Role of Pressure and Duration of Dive on Lung Physiology in Professional and Non-Professional Divers

Jurnal Respirologi Indonesia ◽

10.36497/jri.v40i2.104 ◽

2020 ◽

Vol 40 (2) ◽

pp. 103-112

Author(s):

Jerry Indra Setiawan ◽

Suradi Suradi ◽

Yusup Subagio Sutanto

Keyword(s):

Vital Capacity ◽

Cross Sectional Study ◽

Professional Group ◽

Sectional Study ◽

Test Statistic ◽

Cross Sectional ◽

Lung Physiology ◽

Significant Difference ◽

Normally Distributed

Backgrounds: Professional divers have a greater lung volume than non-professional divers in higher force vital capacity (FVC) and force expiratory flow in 1 second (FEV1) values. The purpose of this study is to analyse the effect of pressure and duration of diving on changes in lung physiology in professional divers and non-professional divers. Methods: This is a cross sectional study on the personnel of the Indonesian Navy Dislambair Koarmada II Surabaya and RSAL personnel of dr. Ramelan Navy Hospital in Lakesla Surabaya in March-April 2019. Samples was collected with purposive sampling. This study used unpaired subjects with the independent t-test statistic analysis if the data is normally distributed and the Mann-Whitney U test if the data is not normally distributed Results: There was a significant difference in the professional divers group compared to non-professional divers group in the values of FVC (P=0.042) and FEV1 (P=0.040) at 1.3 ATA for 10 minutes and the FEV1 (P=0.049) for 20 minutes. No significant differences in FVC (P=0.092) at 1.3 ATA pressure for 20 minutes. There were no significant differences in FVC (P=0.865), FEV1 (P=0.659) at 1.5 ATA pressure for 10 minutes and FVC (P=0.858) and FEV1 (P=0.857) for 20 minutes. Conclusions: The different pressure in non-professional group could reduce the FEV1 value. There was a difference in the FVC value of the professional diver group and non-professional divers at a pressure of 1.5 ATA for 10 minutes. (J Respir Indo. 2020; 40(2): 103-12)

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Leptospira spp. seroprevalence in humans involved in a cross-sectional study in Garissa and Tana River Counties, Kenya

10.1101/2020.07.17.208363 ◽

2020 ◽

Author(s):

Arithi Mutembei ◽

Festus K. Mutai ◽

Damaris Mwololo ◽

John Muriuki ◽

Mark Obonyo ◽

...

Keyword(s):

Case Fatality ◽

Cross Sectional Study ◽

Wilcoxon Test ◽

Categorical Variables ◽

P Value ◽

Continuous Variables ◽

Sectional Study ◽

Test Statistic ◽

Cross Sectional ◽

Tana River

AbstractIntroductionLeptospirosis is a neglected bacterial zoonotic infection caused by spirochetes of Leptospira genus. Humans get infected through direct or indirect contact with urine of infected animals or environment. It accounts for more than 300,000 severe cases annually worldwide with case fatality rates of over 30%. Costs of diagnosis and treatment for human and animals, disruption of international trade of animals and products, reduced productivity and reproductivity in animals constitute economic importance. In Kenya, leptospirosis burden is significant but under-diagnosis and under-reporting affects the awareness of the disease. This study aimed to determine and compare the sero-prevalence and factors associated with Leptospira spp. in the two counties.MethodsWe conducted a cross-sectional study that involved apparently healthy people of at least 5 years of age in randomly selected households in Garissa and Tana River Counties. Blood samples were collected and tested for Leptospira spp antibodies using IgM ELISA. Standardized structured questionnaires were administered to collect socio-demographic and exposure information. We calculated frequencies and proportions for categorical variables and odds ratios (OR) and 95% confidence interval (CI) to evaluate association between sero-positivity and exposure factors. We used Wilcoxon test to evaluate statistical difference in sero-positivity for continuous variables and calculated test statistic (H) and p-value.ResultsA total of 952 subjects were recruited into the study – these included 482 persons from Garissa and 470 from Tana River. The overall sero-prevalence was 26% [(244/952); (CI: 23% to 29%)]. Garissa County had significantly higher Leptospira spp. seroprevalence (31%, n = 147; CI: 27% to 35%) compared to Tana River County (21 %, n = 97; CI: 17% to 25%). Being a female (OR=1.6, CI: 1.2-2.2) and engaging in pastoralism (OR=2.7, CI: 1.8-3.9) were significantly associated with higher odds of Leptospira spp. seropositivity compared to being a male or working in irrigated areas. The mean altitude of residence of sero-positive patients was 73m ± 21 SD (standard deviation) above sea level and that for sero-negative was 80m ± 22 SD (H=35, p-value = 0.00).ConclusionThis study determined the seroprevalence and risk factors for Leptospira spp. exposure in Garissa and Tana River Counties, Kenya. Females in pastoral communities experience high burden of the disease. Enhanced surveillance in humans and animals and further research is required to understand the complex and multifactorial drivers of leptospirosis transmission in the two Counties.

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Malondialdehyde Levels in Preeclampsia before and after Delivery

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v6i3.777 ◽

1970 ◽

pp. 143

Author(s):

Harold Rumopa ◽

Freddy W. Wagey ◽

Eddy Suparman

Keyword(s):

Oxidative Stress ◽

Plasma Levels ◽

Cross Sectional Study ◽

Hplc Method ◽

Wilcoxon Test ◽

Sectional Study ◽

Cross Sectional ◽

Average Value ◽

Significant Difference ◽

Before And After

Objective: Determine differences plasma levels MDA in preeclampsiabefore and 2 hours after delivery. Methods: This was an analytic cross-sectional study. Subjectconsists of 23 pregnancies with preeclampsia, where 23 bloodsamples taken before delivery and 23 were taken 2 hours afterdelivery. This study was conducted from August 2016 untilDecember 2016 at Department of Obstetrics and GynecologyFaculty of Medicine Universitas Sam Ratulangi / Prof. Dr. R. D.Kandou Hospital Manado and satellite hospital. Samples weretaken from plasma and analysed using HPLC method at Prodiaclinical laboratory. Results: In patients with severe preeclampsia before deliverywe found average value (1.4796  0.40819 nmol/ml), minimumvalue (1.03 nmol/ml) and maximal value (2.77 nmol/ml)and 2 hours after delivery with average value (1.2470 0.34324 nmol/ml), minimum value (0.91 nmol/ml), and maximumvalue (2.47 nmol/ml). by using Wilcoxon test, we foundthere were significant differences in plasma levels of MDA (p =0.000). Conclusion: This significant difference suggests that decreasedplasma levels of MDA 2 hours after delivery and gives the sense thatthere is a relationship between oxidative stress of cells with severepreeclampsia before and shortly after delivery, that MDA is an indicatorof oxidative stress. Keywords: malondialdehyde, oxidative stress, peroxidation lipid,preeclampsia

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Comparison of predicted psychological distress among workers between artificial intelligence and psychiatrists: a cross-sectional study in Tsukuba Science City, Japan

BMJ Open ◽

10.1136/bmjopen-2020-046265 ◽

2021 ◽

Vol 11 (6) ◽

pp. e046265

Author(s):

Shotaro Doki ◽

Shinichiro Sasahara ◽

Daisuke Hori ◽

Yuichi Oi ◽

Tsukasa Takahashi ◽

...

Keyword(s):

Neural Network ◽

Artificial Intelligence ◽

Psychological Distress ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Explanatory Variables ◽

The Neural Network ◽

Significant Difference ◽

Total Data

ObjectivesPsychological distress is a worldwide problem and a serious problem that needs to be addressed in the field of occupational health. This study aimed to use artificial intelligence (AI) to predict psychological distress among workers using sociodemographic, lifestyle and sleep factors, not subjective information such as mood and emotion, and to examine the performance of the AI models through a comparison with psychiatrists.DesignCross-sectional study.SettingWe conducted a survey on psychological distress and living conditions among workers. An AI model for predicting psychological distress was created and then the results were compared in terms of accuracy with predictions made by psychiatrists.ParticipantsAn AI model of the neural network and six psychiatrists.Primary outcomeThe accuracies of the AI model and psychiatrists for predicting psychological distress.MethodsIn total, data from 7251 workers were analysed to predict moderate and severe psychological distress. An AI model of the neural network was created and accuracy, sensitivity and specificity were calculated. Six psychiatrists used the same data as the AI model to predict psychological distress and conduct a comparison with the AI model.ResultsThe accuracies of the AI model and psychiatrists for predicting moderate psychological distress were 65.2% and 64.4%, respectively, showing no significant difference. The accuracies of the AI model and psychiatrists for predicting severe psychological distress were 89.9% and 85.5%, respectively, indicating that the AI model had significantly higher accuracy.ConclusionsA machine learning model was successfully developed to screen workers with depressed mood. The explanatory variables used for the predictions did not directly ask about mood. Therefore, this newly developed model appears to be able to predict psychological distress among workers easily, regardless of their subjective views.

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Prevalence of dysmenorrhoea, associated risk factors and its relationship with academic performance among graduating female university students in Ethiopia: a cross-sectional study

BMJ Open ◽

10.1136/bmjopen-2020-043814 ◽

2021 ◽

Vol 11 (3) ◽

pp. e043814

Author(s):

Mesfin Tadese ◽

Andargachew Kassa ◽

Abebaw Abeje Muluneh ◽

Girma Altaye

Keyword(s):

Risk Factors ◽

Academic Performance ◽

University Students ◽

Cross Sectional Study ◽

Secondary Outcome ◽

Sectional Study ◽

Cross Sectional ◽

Grade Point ◽

Significant Difference ◽

Associated Risk Factors

ObjectivesThe study aimed to provide an association between dysmenorrhoea and academic performance among university students in Ethiopia. Further, the study attempts to determine the prevalence and associated risk factors of dysmenorrhoea.Design and methodInstitution-based cross-sectional study was conducted from 1 April to 28 April 2019. A semistructured and pretested self-administered questionnaire was used to collect data. Binary logistic regression analysis and one-way analysis of variance were performed to model dysmenorrhoea and academic performance, respectively.Setting and participantsEthiopia (2019: n=647 female university students).OutcomesThe primary outcome is dysmenorrhoea, which has been defined as painful menses that prevents normal activity and requires medication. The self-reported cumulative grade point average of students was used as a proxy measure of academic performance, which is the secondary outcome.ResultsThe prevalence of dysmenorrhoea was 317 (51.5%). The educational status of father (adjusted OR (AOR) (95% CI) 2.64 (1.04 to 6.66)), chocolate consumption (AOR (95% CI) 3.39 (95% 1.28 to 8.93)), daily breakfast intake (<5 days/week) (AOR (95% CI) 0.63 (0.42 to 0.95)), irregular menstrual cycle AOR (95% CI) 2.34 (1.55 to 3.54)) and positive family history of dysmenorrhoea AOR (95% CI) 3.29 (2.25 to 4.81)) had statistically significant association with dysmenorrhoea. There was no statistically significant difference in academic performance among students with and without dysmenorrhoea (F (3611)=1.276, p=0.28)).ConclusionsDysmenorrhoea was a common health problem among graduating University students. However, it has no statistically significant impact on academic performance. Reproductive health officers should educate and undermine the negative academic consequences of dysmenorrhoea to reduce the physical and psychological stress that happens to females and their families.

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Evaluation of e-health (Seha) application: a cross-sectional study in Saudi Arabia

BMC Medical Informatics and Decision Making ◽

10.1186/s12911-021-01437-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Abeer Alharbi ◽

Joharah Alzuwaed ◽

Hind Qasem

Keyword(s):

Saudi Arabia ◽

Health Services ◽

Healthcare Services ◽

Cross Sectional Study ◽

Advanced Technology ◽

Sectional Study ◽

Cross Sectional ◽

Technical Problems ◽

Significant Difference ◽

Ease Of Access

Abstract Background The Ministry of Health in Saudi Arabia is expanding the country’s telemedicine services by using advanced technology in health services. In doing so, an e-health application (app), Seha, was introduced in 2018 that allows individuals to have face-to-face visual medical consultations with their doctors on their smartphones. Objective This study evaluated the effectiveness of the app in improving healthcare delivery by ensuring patient satisfaction with the care given, increasing access to care, and improving efficiency in the healthcare system. Methods A cross-sectional study design was used to assess the perceptions of users of the Seha app and non-users who continued with traditional health services. The data were collected using an online survey via Google Forms between June 2020 and September 2020. Independent t tests and chi-square (χ2) tests were conducted to answer the research questions. Results There was a significant difference between users and non-users in terms of ease of access to health services (t = − 9.38, p < 0.05), with app users having a higher mean score (4.19 ± 0.91) than non-users (3.41 ± 1.00); satisfaction with health services (t = − 6.33, p < 0.05), with users having a higher mean score (3.96 ± 0.91) than non-users (3.45 ± 0.94); and efficiency (only one visit needed for treatment) (t = − 3.20, p < 0.05), with users having a higher mean score (3.71 ± 0.93) than non-users (3.45 ± 0.93). There were significant associations between the use of the Seha app and age (χ2 = 8.79, p < 0.05), gender (χ2 = 22.19, p < 0.05), region (χ2 = 19.74, p < 0.05), and occupation (χ2 = 22.05, p < 0.05). There were significant relationships between the three items (on access, satisfaction, and efficiency) and experiencing technical issues (t = 4.47, t = 8.11, and t = 3.24, respectively, p < 0.05), with users who faced technical problems having significantly lower mean scores for all three items. Conclusion This study provided evidence that the Seha app improved the delivery of healthcare in Saudi Arabia. Users of the app had a better health experience in terms of their perceived ease of access to healthcare services; their satisfaction with healthcare services; and the efficiency of the system, measured by the number of required doctor visits. Other factors that appeared to influence the use of the app included age, gender, usual source of care, and technical difficulties.

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P20 Parental understanding of concussion in youth rugby is significantly improved by viewing the RFU concussion education video

Journal of Neurology Neurosurgery & Psychiatry ◽

10.1136/jnnp-2019-abn.97 ◽

2019 ◽

Vol 90 (3) ◽

pp. e31.1-e31

Author(s):

T Samuel ◽

K Aquilina ◽

W Dawes

Keyword(s):

Parental Education ◽

Cross Sectional Study ◽

Educational Video ◽

Sectional Study ◽

Online Questionnaire ◽

Cross Sectional ◽

Parental Experience ◽

Increased Risk ◽

Significant Difference ◽

Composite Scores

ObjectivesTo investigate the current understanding parents have of concussion in their rugby-playing children aged 9–17.DesignCross-sectional studySubjects86 parents from four clubs completed an online questionnaireMethodsAreas covered (1) Parental experience of concussion (2) Rate of viewing of the RFU concussion educational video (RFUCEV), (3) Identification of symptoms of concussion (4) Understanding of the risk of concussion. Each participant was given a composite score, out of 19, based on their performance in symptom identification and true/false questions. Independent-sample two-tailed t-tests were conducted to analyse scores, primarily controlling for viewing of the RFUCEVResultsThere was a significant difference in the scores between the group that had viewed the RFUCEV (n=32, M=14.75, SD=2.55) and those who had not (n=54, M=13.05, SD=2.87); t(84)=2.75, p=0.00721. Additionally, over 25% of parents reported that their child had suffered a concussion, and this was also found to significantly improve the awareness score (p=0.04678)ConclusionsStatistically significant improvement in composite scores after viewing the RFUCEV makes it reasonable to require compulsory parental education prior to signing up a child to play rugby. This would build on the protocol changes made by the RFU in 2014 and further progress the safety of the sport. We suggest the video be improved to emphasise the areas highlighted as inadequately understood, including the increased risk of concussion in under-18s compared to adults.

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Factors associated with physical inactivity among female and male rural adolescents in Borneo – a cross-sectional study

International Journal of Adolescent Medicine and Health ◽

10.1515/ijamh-2013-0319 ◽

2014 ◽

Vol 26 (3) ◽

pp. 447-453 ◽

Cited By ~ 3

Author(s):

Whye Lian Cheah ◽

Hazmi Helmy ◽

Ching Thon Chang

Keyword(s):

Self Efficacy ◽

Physical Inactivity ◽

Cross Sectional Study ◽

Perceived Barriers ◽

Anthropometric Measurement ◽

Rural Adolescents ◽

Sectional Study ◽

Cross Sectional ◽

Factors Associated ◽

Significant Difference

Abstract Rural communities have shown marked increase in metabolic syndrome among young people, with physical inactivity as one of the main contributing factors. This study aimed to determine factors associated with physical inactivity among male and female rural adolescents in a sample of schools in Malaysia. A cross-sectional study was conducted among 145 students aged 13–15 years. Data on socio-demographic, health-related, and psychosocial factors (perceived barriers, self-efficacy, social influences) were collected using a self-administered questionnaire. Anthropometric measurement was taken to generate body mass index (BMI)-for-age, while physical activity (PA) level was assessed using pedometers. The mean steps per day was 6251.37 (SD=3085.31) with males reported as being more active. About 27% of the respondents were either overweight or obese, with more females in this group. There was no significant difference in steps among males and females (p=0.212), and nutritional status (BMI-for-age) (p=0.439). Females consistently scored higher in most items under perceived barriers, but had significantly lower scores in self-efficacy’s items. Males were more influenced by peers in terms of PA (p<0.001) and were more satisfied with their body parts (p=0.047). A significantly higher body size discrepancy score was found among females (p=0.034, CI –0.639, –0.026). PA level was low and almost one-third of the respondents were overweight and obese. Female students faced more barriers and had lower self-efficacy with regards PA. Based on the findings, it is recommended that interventions focus on reducing barriers while increasing support for PA. This is particularly important in improving the health status of the youth, especially among the females.

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Awareness of basic life support among undergraduate medical students in Syria, Iraq and Jordan: a cross sectional study

10.21203/rs.3.rs-315179/v1 ◽

2021 ◽

Author(s):

Mohammad Nour Shashaa ◽

Mohamad Shadi Alkarrash ◽

Mohammad Nour Kitaz ◽

Roaa Rhayim ◽

Mohammed Ismail ◽

...

Keyword(s):

Medical Students ◽

Basic Life Support ◽

Life Support ◽

Clinical Stage ◽

Sudden Cardiac Arrest ◽

Cross Sectional Study ◽

Sectional Study ◽

Undergraduate Medical Students ◽

Cross Sectional ◽

Significant Difference

Abstract Background Sudden cardiac arrest considers one of the most leading cause for death in all over the world. It is important for all medical students to train basic life support. This study evaluated the awareness of basic life support among medical students. Methods An electronic questionnaire based cross sectional study was conducted in November 2020 among 2114 medical student in Syria, Iraq and Jordan. We evaluated BLS skills according to mean score. A chi-squared test was used to determine if there were differences between those who attended a basic life support course and those who did not. Results 1656 of the participants (78.3%) stated that they did not attend a basic life support course. There was a significant difference between the participants from different countries where the mean score in Syria, Jordan and Iraq was 18.3, 24.3 and 18.8 respectively (p < 0.05). The participants were divided into 3 level according to total score; low (0–12), moderate (13–24) and high (25–37). In total, 18.3%, 72.8% and 8.9% of participants had high, intermediate and low level respectively. Conclusions The overall knowledge of basic life support among medical students is not adequate and need significant improvements. This study showed that an attendance a basic life support course previously had an effect on knowledge level. Hence, there is an urgent need to apply basic life support courses into the pre-clinical stage at universities.

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Denver Developmental Screening Test in two-year old infants delivered by vacuum extraction

Paediatrica Indonesiana ◽

10.14238/pi41.1.2001.27-32 ◽

2017 ◽

Vol 41 (1) ◽

pp. 27

Author(s):

Meriah Sembiring ◽

Iskandar Iskandar ◽

Amir Syarifuddin ◽

Bistok Saing

Keyword(s):

Physical Examination ◽

Screening Test ◽

Cross Sectional Study ◽

Developmental Screening ◽

Vacuum Extraction ◽

Sectional Study ◽

Chi Square ◽

Cross Sectional ◽

Developmental Retardation ◽

Significant Difference

The aim of this study was to determine the developmental retardation of infants of two years of age who were delivered by vacuum extraction. This cross-sectional study examined 44 infants delivered by vacuum extraction, comprising 25 males and 19 females who were born in Tembakau Deli and St. Elizabeth Hospitals, between August 1993 until February 1994. The examination included interview and physical examination in the patient's house. Chi-square statistics analysis was used with a significant level of 95% (1'=0.05). The results showed Ihat of the 44 infants delivered by vacuum extraction. 28 (32%) had had were found with mild asphyxia, while 2 infants (5%). whose mothers work as private clerk and entrepreneur, had development retardation. We concluded that there was no significant difference in development between infants delivered by vacuum extraction and those who were born spontaneously. Developmental retardation was found in infants whose mothers lack time to communicate.

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