scholarly journals Yield, chemical composition and functional properties of essential oils from Mentha spicata (Lamiaceae) in Santiago del Estero, Argentina

2019 ◽  
Vol 11 (3) ◽  
pp. 327-333
Author(s):  
Diego Ariel Meloni ◽  
Julia Andrea Lescano ◽  
Maria Paz Arraiza ◽  
Rosa Elizabeth Beltrán
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Pharmaceutical Products ◽  
Acetylcholinesterase Inhibition ◽  
Mentha Spicata ◽  
Essential Components ◽  
Dpph Method ◽  
Ic50 Values ◽  
High Antioxidant Activity

Introduction: Mentha spicata, commonly known as spearmint, is the most common and popular cultivated mint, and is used in food, perfumes and pharmaceutical products. Objective: To determine the yield, composition and properties of essential oil from M. spicata grown in Santiago del Estero, Argentina. Methods: We used steam distillation, gas chromatography and mass spectrometry to determine chemical composition, antioxidant activity and acetylcholinesterase inhibition. Results: Essential oil yield ranged from 0.8 to 1.3 mL·100 g-1 DM. The main essential components were carvone (60,72-68,09%), limonene (14,23-16,41%), α-pinene (0,12-3,14%), β-myrcene (0,35-2,33%), 1,8-cineole (1,69-2,73%), cis-dihydrocarvone (1,82-2,31%), trans-craveol (1,09-1,19%), β-bourbonene (2,44-3,17%) and β-Caryophyllene (1,42-1,82%). The oil had high antioxidant activity, with IC50 values among 7,5-12,06 µg/mL (DPPH method) and 26,94-38,14 µg/mL (β-carotene/linoleic acid method). It had good inhibition of acetylcholisterenase, with enzyme inhibition zones of 0,4-0,9 cm. Conclusion: The yield, quality and properties of the essential oil from M. spicata grown in Santiago del Estero meet market requirements.

scholarly journals Chemical Composition and Antioxidant Activity of Thymus fontanesii Essential Oil from Algeria

2020 ◽  
Vol 10 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Sid Ali Lamia ◽  
Brada Moussa ◽  
Fauconnier Marie-Laure ◽  
Lognay Georges
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Electromagnetic Induction ◽  
Reducing Power ◽  
Gas Chromatography Mass Spectrometry ◽  
The People ◽  
Assisted Extraction ◽  
Ic50 Values

Background: Thymus fontanesii is one of the important Algerian plants, used traditionally to treat the cough and cold. In addition, it may help to protect the people against lipid peroxidation and oxidative stress and can be used as an antioxidant agent for the preservation of processed food. Objective: The aim of this study was to determine the chemical composition of Algerian Thymus fontanesii essential oil and to test its antioxidant activity. Methods: The oil was extracted by electromagnetic induction (EMI) heating assisted extraction and by hydrodistillation, and was analysed by Gas Chromatography with Flame Ionization Detector (GC/FID) and Gas Chromatography/Mass Spectrometry (GC/MS). The antioxidant activity was evaluated by three assays mainly: DPPH assay, reducing power and β-carotene/linoleic acid. Results: The yield of the essential oil was varied from 2.1 ± 0.3 to 3.1 ± 0.1% (w/w), and from 1.8 ± 0.01 to 2.6 ± 0.02% (w/w), for the electromagnetic induction heating assisted extraction and hydrodistillation, respectively. Twenty seven components were identified representing 95.6 - 99.9% of the oil. Carvacrol (54.7 ± 1.2 - 63.9 ± 1.9%) was the major compound followed by p-cymene (9.2 ± 1.2 - 17.5 ± 1.2%) and γ-terpinene (8.8 ± 0.9 - 14.9 ± 0.8%). The Thymus fontanesii essential oil was found as a significant antioxidant with IC50 values ranging from 57.3 ± 1.4 to 236.7 ± 1.4 μg/mL, which were higher than that of butylated hydroxyl toluene (BHT) choosing as reference (9.1 ± 1.2 to 67.8 ± 0.1 μg/mL). Conclusion: The obtained results encourage the use of Thymus species with bioactive compounds for further food applications.

scholarly journals Acetylcholinesterase Inhibition and Antioxidant Activity of Essential Oils from Schinus Areira L. and Schinus Longifolia (Lindl.) Speg

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ana P. Murray ◽  
María S. Vela Gurovic ◽  
Silvana A. Rodriguez ◽  
María G. Murray ◽  
Adriana A. Ferrero
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Acetylcholinesterase Inhibition ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.

scholarly journals Formulasi Masker Gel Antioksidan Mengandung Ekstrak Kulit Buah Naga Merah (Hylocereus polyrhizus)

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Sunarmi Sunarmi ◽  
Susilo Yulianto
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Fruit Peel ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Before And After ◽  
The Face ◽  
Ic50 Values ◽  
High Antioxidant Activity ◽  
Dispersive Power

Abstract: Antioxidant, Red Dragon Fruit, Gel Mask. Skin is an organ that covers the entire human body, which has protection against external influences. Free radicals and the oxidation reaction can be inhibited by an antioxidant compound. Red dragon fruit peel is rarely used but has high antioxidant activity that can be used for skin care, especially facial skin. Red dragon fruit peel can be formulated into various dosage form of antioxidants for the face skin, one of which peel-off mask gel. This study aimed to test the antioxidant activity of peel-off mask gel with the active ingredient of red dragon fruit peel extracts. This research uses experimental methods. Red dragon fruit peel extracted by maceration in 70% ethanol and concentrated. Gel mask made in 3 variations extract concentration, ie formula A, B and C 0.175 g, 0.350 g and 0,700 g respectively. The physical stability test was conducted by cycling test for 6 cycles. The physical properties of the preparation were tested before and after storage include adhesion, dispersive power, pH, viscosity and time to dry. Antioxidant activity tested with DPPH method. Based on test results obtained physical stability, third-formula gel mask physically qualified and stable, except for the dispersive power. Antioxidant activity decreased after the storage. The highest activity was shown by formula C with an IC50 value of 3497.113 ppm. Based on IC50 values, this gel mask has an inactive. antioxidant activity.

scholarly journals Helichrysum italicum (Roth) G. Don subsp. italicum from Herzegovina

2019 ◽  
Vol 92 (1) ◽  
pp. 69-77
Author(s):  
Stanislava Talić ◽  
Ilijana Odak ◽  
Anita Martinović Bevanda ◽  
Nikolina Crnjac ◽  
Mate Paštar
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibition Activity ◽  
Complex Chemical ◽  
Methanolic Extracts ◽  
Complex Chemical Composition ◽  
Ic50 Values ◽  
Vegetative Cycle ◽  
Inhibitory Potential

The chemical composition of essential oils isolated from immortelle (Helicrysum italicum subsp. italicum) collected in Herzegovina during five different periods, was investigated by GC/MS analysis. The main compounds were a-pinene (15.7 %) and γ-curcumene (12.8 %), followed by 4,6,9-trimethyldec-8-en-3,5-dione (8.7 %), neryl acetate (6.9 %), limonene (6.4 %) and β-selinene (5.3 %). In total, 69 components were identified whose share changed over the vegetative cycle. Antioxidant activity of methanolic extracts of immortelle were determined according to DPPH (IC50 = 23–34 μg/mL) and FRAP (29 μg/mL is equivalent to 1.1‒2.2 mM Fe2+) methods. Acetylcholinesterase inhibitory potential, investigated by modified Ellman’s assay and determined as IC50 values, were 340–440 μg/mL for methanol extracts and 135 μg/mL for essential oil. Metanolic extracts showed strong antioxidant activity and potential to inhibit AChE. Essential oil possesses complex chemical composition, inhibition activity of AChE and weak antioxidant capacity.

scholarly journals Chemical composition, antimicrobial and antioxidant activity of the essential oil of leaves of Eugenia involucrata DC.

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Adrieli Gorlin Toledo ◽  
Juliete Gomes de Lara de Souza ◽  
Jéssica Patrícia Borges da Silva ◽  
Wagner Alex Jann Favreto ◽  
Willian Ferreira da Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Potential ◽  
Gram Positive Bacteria ◽  
Dpph Method ◽  
Germacrene D ◽  
Antimicrobial And Antioxidant Activity ◽  
Reduction Capacity

In the Myrtaceae family, the species Eugenia involucrata DC., popularly known as "cerejeira-do-mato", is traditionally used for the antidiarrheal and digestive action of its leaves. However, no studies were found in the literature regarding its antimicrobial and antioxidant potential. In this context, the objective of the present study was to determine the chemical composition by gas chromatography coupled to mass spectrometry (GC-MS) to evaluate the antimicrobial activity by the broth microdilution technique and the antioxidant activity by the 2,2-diphenyl-1-picryl-hydrazila (DPPH) method of the essential oil of E. involucrata leaves. GC-MS identified 28 compounds, all sesquiterpenes, corresponding to 89.41% of the essential oil. The antimicrobial activity of the essential oil was observed for all Gram-positive bacteria tested (Staplylococcus epidermidis, Enterococcus faecalis, Bacillus subtilis and Staplylococcus aureus) and for yeast Candida albicans. The essential oil presented a reduction capacity of DPPH up to 66.81%, evidencing its antioxidant potential. It is suggested that the antimicrobial and antioxidant action of E. involucrata essential oil is related to the presence of the major compounds, elixene (26.53%), β-caryophyllene (13.16%), α-copaene (8.41%) and germacrene D (7.17%).

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Chemical Composition and Antibacterial and Antioxidant Activity of a Citrus Essential Oil and Its Fractions

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2888
Author(s):  
Carmen M. S. Ambrosio ◽  
Gloria L. Diaz-Arenas ◽  
Leidy P. A. Agudelo ◽  
Elena Stashenko ◽  
Carmen J. Contreras-Castillo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
E Coli ◽  
Beneficial Bacterium ◽  
Minor Compounds ◽  
Antibacterial And Antioxidant Activity ◽  
High Relative Amount

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

scholarly journals Impact of Harvest Conditions and Host Tree Species on Chemical Composition and Antioxidant Activity of Extracts from Viscum album L.

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3741
Author(s):  
Wioleta Pietrzak ◽  
Renata Nowak
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Chemical Composition ◽  
Tree Species ◽  
Plant Material ◽  
Plant Secondary Metabolites ◽  
Host Tree ◽  
Host Tree Species ◽  
High Antioxidant Activity ◽  
Mistletoe Extracts

The content of plant secondary metabolites is not stable, and factors such as the region/location effect and seasonal variations have an impact on their chemical composition, especially in parasitic plants. Research in this area is an important step in the development of quality parameter standards of medicinal plants and their finished products. The effects of the time and place of harvest and the host tree species on the chemical composition and antioxidant activity of mistletoe extracts were investigated. Statistical tools were used to evaluate the results of the spectrophotometric and LC-ESI-MS/MS studies of the phenolic composition and antioxidant activity. The investigations indicate that the qualitative and quantitative composition, influencing the biological activity of mistletoe extracts, largely depends on the origin of the plant. The mistletoe extracts exhibited a rich phenol profile and high antioxidant activity. The chemometric analysis indicated that mistletoe collected from conifers (Viscum abietis and Viscum austriacum) had the most advantageous chemical composition and antioxidant activity. Moreover, the chemical profile and biological activity of the plant material were closely related to the climatic conditions and location of the harvested plant. Higher levels of phenolic compounds and high antioxidant activity were found in extracts obtained from plant material collected in cold weather with the presence of snow and less sunshine (autumn–winter period).

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