scholarly journals Pengujian Sifat Fisik dan Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Daun Lidah Mertua (Sansevieria trifasciata Prain)

2018 ◽  
Vol 1 (3) ◽  
pp. 078-085
Author(s):  
Setyo Nurwaini ◽  
Intan Dewi Saputri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Properties ◽  
Dosage Form ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Diffusion Method ◽  
Hand Sanitizer ◽  
Significant Difference

Daun Lidah mertua (Sansevieria trifasciata Prain) diketahui mengandung senyawa polifenol, flavonoid dan saponin yang merupakan agen antibakteri. Lidah mertua memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengetahui konsentrasi ekstrak daun lidah mertua dalam sediaan gel yang memiliki aktivitas antibakteri terbesar dalam penurunan jumlah angka kuman. Ekstraksi daun lidah mertua menggunakan metode maserasi dengan pelarut etanol 96%. Variasi konsentrasi ekstrak daun lidah mertua dalam sediaan gel hand sanitizer yang digunakan yaitu F1 (10%), F2 (15%) dan F3 (20%). Na CMC digunakan sebagai gelling agent dalam formulasi. Sediaan gel hand sanitizer daun lidah mertua diuji aktivitas antibakteri terhadap Escherichia coli menggunakan metode difusi sumuran, sifat fisik (organoleptik, homogenitas, pH, viskositas, daya sebar dan daya lekat) serta penurunan jumlah angka kuman dengan metode swabbing. Hasil uji aktivitas antibakteri gel hand sanitizer ekstrak daun lidah mertua memiliki zona hambat bakteri terhadap Escherichia coli pada F1, F2 dan F3 berturut-turut sebesar 7,33 mm, 8,67 mm dan 9,75 mm. Hasil evaluasi sifat fisik gel daun lidah mertua memenuhi kriteria gel yang baik, namun memiliki daya lekat kurang 1 detik. Hasil penurunan jumlah angka kuman gel hand sanitizer daun lidah mertua paling besar pada F3 sebesar 52%. Namun, analisa statistik dengan Anova One Way menunjukkan bahwa F1 (32%), F2 (35%) dan F3 (52%) tidak menunjukkan perbedaan yang signifikan dalam penurunan jumlah angka kuman. Berdasarkan hasil penelitian ini dapat disimpulkan gel hand sanitizer ekstrak daun lidah mertua memiliki kemampuan dalam menurunkan angka kuman dan dapat digunakan sebagai alternatif sebagai sediaan gel hand sanitizer.     Lidah Mertua leaves (Sansevieria trifasciata Prain) are known to contain polyphenols, flavonoids, and saponins which have antibacterial activity. Lidah Mertua leaves have antibacterial activity against Staphylococcus aureus and Escherichia coli. This study aimed to determine the concentration of Lidah Mertua leaves extract in a gel dosage form which showed the highest antibacterial activity in decreasing germ numbers. Lidah Mertua leaves extract was prepared using the maceration method with ethanol 96%. There was a various concentration of Lidah Mertua leaves extract in gel hand sanitizer, these include F1 (10%), F2 (15%) and F3 (20%). Na CMC is used as a gelling agent in the formulation. The evaluation of the antibacterial activity of hand sanitizer gel leaves was performed against Escherichia coli using disc diffusion method, meanwhile physical properties (organoleptic, homogeneity, pH, viscosity, dispersion and adhesion) and a decrease in the germ numbers were determined by swabbing method. The results of the antibacterial evaluation of gel hand sanitizer activity showed that lidah Mertua leaves extract had a bacterial inhibition zone against Escherichia coli in F1, F2 and F3 of 7.33 mm, 8.67 mm and 9.75 mm respectively. The results of the evaluation of the physical properties of the lidah mertua leaves extract showed that the gel met criteria of a good gel, but had adhesion less than 1 second. The highest activity in decreasing term number was shown in gel hand sanitizer of lidah mertua leaves extract at F3 which was 52%. However, statistical analysis using Anova One Way showed that F1 (32%), F2 (35%) and F3 (52%) did not show a significant difference in decreasing germ numbers. Based on the results of this study, it can be concluded that the  hand sanitizer gel from the extract of lidah mertua leaves has the ability to reduce the number of germs and can be used as an alternative as a gel hand sanitizer dosage form

scholarly journals Formulation of Gel Hand Sanitizer of Nagasari Leaf Extract (Mesua ferrea L.)

2022 ◽  
Vol 1 ◽  
pp. 31
Author(s):  
Tuti Sri Suhesti ◽  
M. Mudrik H. Rohman ◽  
Sunarto Sunarto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Properties ◽  
Active Substance ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Stability Testing ◽  
Hand Sanitizer ◽  
Flavonoid Compounds

Nagasari (Mesua ferrea L.) is one of the biodiversity to be developed as an antiseptic preparation. These plants are known to contain flavonoid compounds, tannins, and terpenoids that act as antibacterial. Hand sanitizer gel preparations can increase the effectiveness of topically. The physical properties of a good gel depend on a gelling agent, one of which is HPMC. The purpose of this study was to determine the effect of variations in HPMC levels on physical properties and antibacterial activity. Gels were prepared with various HPMC levels of 1%, 2%, and 3%. The gel was tested for physical properties and stability. All formulas produced preparations that met the requirements for good physical properties and stability. Testing of antibacterial activity against Staphylococcus aureus showed that an increase in HPMC levels could decrease the ability to release the active substance of the preparation. The diameter of the inhibition zone obtained was 10.0 mm (HPMC 1%); 9.5 mm (2% HPMC) and 8.0 mm (3% HPMC). Increasing the concentration of HPMC will increase the viscosity and adhesion but decrease the spreadability. The three formulas had antibacterial activity against Staphylococcus aureus with moderate criteria.Keywords: Extract of nagasari leaf, Gel, HPMC, Staphylococcus aureus

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract on Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 3 (2) ◽  
pp. 26
Author(s):  
Asman Sadino ◽  
Idin Sahidin ◽  
Wahyuni Wahyuni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Weak Antibacterial Activity

The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique

scholarly journals Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Nurul Cholifah ◽  
Ahmad Ridhay ◽  
Pasjan Satrimafitrah ◽  
Ruslan ◽  
Hardi Ys
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

scholarly journals PENGARUH VARIASI GELLING AGENT KARBOMER 934 EKSTRAK ETANOL DAUN JERUK PURUT (Citrus hystrix DC) TERHADAP SIFAT FISIK GEL DAN AKTIVITAS ANTIBAKTERI Staphylococcus aureus

2019 ◽  
Vol 4 (1) ◽  
pp. 248-259
Author(s):  
Anggun Hari Kusumawati ◽  
Siti Hufi Hutami
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Topical Product ◽  
Gel Preparation ◽  
Made In

ABSTRAK Ekstrak etanolik daun jeruk purut (Citrus hystrix D.C) mempunyai banyak zat khasiat dimana salah satu zat aktif tersebut berkhasiat sebagai antibakteri. Penggunaan ekstrak etanolik dalam bentuk kental sangat tidak efisien dalam pemakaiannya, sehingga perlu dibuat dalam bentuk sediaan topical, misalnya gel. Penggunaan Karbomer 934 sebagai gelling agent dapat meningkatkan konsistensi basis yang akan berpengaruh terhadap pelepasan zat aktif di dalam gel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi kadar basis Karbomer 934 gel ekstrak etanolik daun jeruk purut terhadap sifat fisik gel dan aktivitas antibakteri terhadap Staphylococcus aureus. Gel dibuat dalam 4 formula dengan konsentrasi basis Karbomer 934 yaitu 0,5 %, 1%, 1,5%, dan 2%. Gel diuji sifat fisik (organolepstis, homogenitas, pH, viskositas, daya lekat, daya sebar, dan uji hedonik) dan daya antibakteri terhadap Staphylococcus aureus. Data dari uji sifat fisik dan pengukuran diameter zona hambat anti bakteri dianalisis dengan statistika Uji Kruskall-Wallis dan Mann-Withney Hasil penelitian dapat disimpulkan bahwa variasi konsentrasi Karbomer 0,5 %, 1 %, 1,5 % dan 2% sebagai gelling agent gel ekstrak etanol daun jeruk purut berpengaruh terhadap sifat fisik gel dan aktifitas antibakteri terhadap Staphylococcus aureus. Semakin besar konsentrasi Karbomer 934, viskositas semakin besar dengan FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, menurunkan daya sebar dengan FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, meningkatakan daya lekat dengan FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm dan menurunkan efektivitas antibakteri dengan FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Kata Kunci : gel, gelling agent, Karbomer 934, Citrus hystrix D.C , Staphylococcus aureus.   ABSTRCT Ethanolic extract of kaffir lime leaves (Citrus hystrix D.C) Has many active compounds, antimicrobial activity have been reported numerously. Ethanolic extract of Kaffir Lime leaves can’t directly exposure to the human skin. It can be increased of skin rash and eritema. The extract can be formulated in topical product like gel preparation. The aim of this work was to determine the effect of variations in base levels of Karbomer 934 gel ethanolic extract of kaffir lime leaves on the physical properties of gel and antibacterial activity against Staphylococcus aureus. The gel is made in 4 formulas with a base concentration of Carbomer 934 which is 0.5%, 1%, 1.5%, and 2%. Gel was tested for physical properties (organolepstis, homogeneity, pH, viscosity, adhesion, dispersion, and hedonic test) and antibacterial power to Staphylococcus aureus. Measuring the diameter of the antibacterial inhibition zone after incubation at 37ºC for 24 hours. Data from the physical properties test and measuring the diameter of the anti-bacterial inhibitory zone were analyzed by statistical with Kruskal-Wallis test and Mann-Withney test. The results of this study concluded that variations in Carbomer 934 concentration as gelling agent gel ethanol extract of kaffir lime leaves had an effect on the physical properties of the gel and antibacterial activity against Staphylococcus aureus. The greater concentration of Carbomer 934, increase the viscosity value with FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, decrease the spreadability with FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, increase in adhesion with FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm and decrease the effectiveness of the antibacterial with FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Keywords: gel, gelling agent, Carbomer 934, Citrus hystrix D.C , Staphylococcus aureus.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals UJI AKTIVITAS ANTIBAKTERI ALGA HIJAU (Ulva sp.) DARI PANTAI SORIDO BIAK TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Melisa Selly Liswandari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Green Algae ◽  
Antibacterial Agent ◽  
Solvent Polarity ◽  
Negative Control ◽  
Diffusion Method ◽  
Significant Difference

UJI AKTIVITAS ANTIBAKTERI ALGA HIJAU (Ulva sp.) DARI PANTAI SORIDO BIAK TERHADAP BAKTERI Escherichia coli  DAN Staphylococcus aureus Melisa Selly Liswandari1), Daniel Lantang2), Septriyanto Dirgantara1)1)Program Studi Farmasi, Fakultas MIPA Universitas Cenderawasih, Jayapura2)Jurusan Biologi, Fakultas MIPA Universitas Cenderawasih, Jayapura, Kampus UNCEN Waena. Jl Perumnas III Waena Jayapura 99351 PapuaEmail*: [email protected]  ABSTRACT The green algae (Ulva sp.) are potentially as antibacterial agent because it contains triterpenoids, flavonoids, and saponins. The objective of this research are to know antibacterial activity of green algae (Ulva sp.) extracts using different levels of solvent polarity and the most effective concentration inhibits the growth of bacterial Escherichia coli and Staphylococcus aureus. Green algae (Ulva sp.) extracted by stratified soxletation method using solvent, eq diethyl ether, ethyl acetate, and ethanol 96%. Test antibacterial activity using the disc diffusion method. This research using the of CRD (Completely Randomize Design) with 7 treatments was concentration of 100 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm, positive control (ciprofloxacin 5µg) and negative control (aquades steril) with 3 repetitions. The data was analyzed using ANOVA and continued by HSD (Honestly Significant Difference) test with 95% confidence level. The result was demonstrated that extract green algae (Ulva sp.) the most effective to inhibits the growth of bacterial Escherichia coli is ethyl acetate extract with concentration 250 ppm (9,26 mm) and bacterial Staphylococcus aureus is ethanol 96% extract with concentration 750 ppm (9,57 mm). Keywords : Ulva sp., antibacterial agent, Escherichia coli, Staphylococcus aureus. ABSTRAK             Alga hijau (Ulva sp.) berpotensi sebagai antibakteri karena mengandung triterpenoid, flavonoid, dan saponin. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak alga hijau (Ulva sp.) yang menggunakan tingkat kepolaran pelarut berbeda dan konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Alga hijau (Ulva sp.) diekstraksi dengan metode sokletasi bertingkat menggunakan pelarut dietil eter, etil asetat, dan etanol 96%. Uji aktivitas antibakteri menggunakan metode difusi cakram. Penelitian ini menggunakan RAL dengan 7 perlakuan yaitu konsentrasi 100 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm, kontrol positif (ciprofloxasin 5µg) dan kontrol negatif (akuades steril) dengan 3 pengulangan. Data analisis menggunakan ANOVA dan dilakukan uji lanjut BNJ dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak alga hijau (Ulva sp.) yang paling efektif untuk menghambat pertumbuhan bakteri Escherichia coli adalah ekstrak etil asetat dengan konsentrasi 250 ppm (9,26 mm) dan bakteri Staphylococcus aureus adalah ekstrak etanol 96% dengan konsentrasi 750 ppm (9,57 mm). Kata kunci : Ulva sp., antibakteri, Escherichia coli, Staphylococcus aureus. 

scholarly journals FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF HAND SANITIZER ETHANOL EXTRACT OF BASIL (Ocimum Sanctum. L) AGAINST Escherichia coli dan Streptococcus Aureus

2020 ◽  
Vol 15 (2) ◽  
pp. 228
Author(s):  
Nurfiddin Farid ◽  
Nurhikma Nurhikma ◽  
Suhartina Hamzah ◽  
Muhammad Yusuf ◽  
Rahmania Rahmania
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Ocimum Sanctum ◽  
Hand Sanitizer ◽  
Activity Test ◽  
Before And After ◽  
And Staphylococcus Aureus

Basil leaves (Ocimum sanctum L) Against to inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria to increase its effectiveness, it is made in practical and easy-to-use preparations. One of the effective dosage forms for topical use is Hand Sanitizer. The factor that influences the quality of gel preparations is Carbopol as a gelling agent. To know the antibacterial effect and ethanol extract concentration of basis leaves (Ocimum sanctum L). preparation of Hand sanitizer, in which the selection and type of carbopol concentration will determine the stability of the gel formed. Basil leaf extract was obtained by maceration method using 96% ethanol solvent. The product is formulation in the form a gel Hand sanitizer with the addition of carbopol as a gelling agent with a concentration of 1,5% this type of research is  laboratory Experiment with Stability Test, Organoleptic Test, pH, Homogeneity and Scattering power and Antibacterial Test on Escherichia coli and Staphylococcus aureus. For testing antibacterial activity the method used is the diffusion method. Form the research results obtained antibacterial Hand sanitizer preparations with concentration of 1%, 3% and 5%  showed changes in odor , color and shape before and after storage at pH 4,8- 6,4. Organoleptic  testing showed no changes in odor, color and shape before and after accelerated storage. pH testing shows charges before and after storage are caused by temperature light factors. Homogeneity testing showed no changes before and after storage. Scatter tests indicate changes before and after storage caused by temperature light factors. Antibacterial activity test shows that the formulation of Hand sanitizer ethanol extract of basil leaves (Ocimum sanctum L) with a concentration of 1%, 3% and 5%. can inhibit the growth of Escherichia coli and Staphylococcus aureus with Medium -Strong inhibitory zones. From the results of research conducted it was concluded that the antibacterial Hand sanitizer ethanol of basis was physically and chemically stable for Organiletic, pH, Homogeneity and Scattering effects.

scholarly journals Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
Hosea Jaya Edy ◽  
Marchaban Marchaban ◽  
Subagus Wahyuono ◽  
Agung Endro Nugroho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %.  Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

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