scholarly journals Changes in the vitality, lipid peroxidation and hydrogen peroxyde content of maize /Zea mays l./ seedlings during controlled accelerated ageing

2021 ◽  
Vol 13 (29) ◽  
pp. 110-117
Author(s):  
Radoslav Chipilski ◽  
◽  
Albena Pencheva ◽  

Seeds from the maize /Zea mays L./ variety Kneja 310 and landrace B0E0170 were subjected to controlled accelerated aging test (AA) for 72 hours and 120 hours at temperature 41±0.5°C and 100.0% air relative humidity. For the control variant, not subjected to the АА test seeds were used. After AA test, all seed variants were germinated and were used for an analysis of germination energy. The emerging coleoptiles were studied for the presence of anti-oxidative response (MDA, hydrogen peroxide, cell membrane stability), and morphological parameters and relative chlorophyll content were assessed as well as. A vegetation pot experiment was performed with the AA-72h variant and 15-day seedlings were morphologically and physiologically assessed. The aim of the study was to evaluate the relationship between the vitality of the seeds and their stress response to the controlled AA test, which simulated low-temperature storage. The germination rate of seeds and the fresh and dry weight of coleoptiles following ageing treatments were decreased. These results correlated well with the increased accumulation of total hydrogen peroxide and MDA content and the decrease of cell membrane stability. Negative changes were observed in chlorophyll content index, fresh and dry weight and leaf area of 15-day seedlings in pot trial. It was concluded that the controlled accelerated ageing could be used as a model for estimation of seed deterioration in maize after long-term storage.

1990 ◽  
Vol 59 (2) ◽  
pp. 354-358 ◽  
Author(s):  
Gnanasiri S. PREMACHANDRA ◽  
Hirohumi SANEOKA ◽  
Hideaki MATSUURA ◽  
Shoitsu OGATA

2017 ◽  
Vol 42 (3) ◽  
pp. 475-485 ◽  
Author(s):  
JA Chowdhury ◽  
MA Karim ◽  
QA Khaliq ◽  
AU Ahmed

An experiment was conducted in a venylhouse at the environmental stress site of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during September to December 2012 to study the effect of drought stress on proline content, soluble sugar content, chlorophyll content and cell membrane stability of soybean genotypes. Four studied genotypes viz., Shohag, BARI Soybean-6 and BD2331 (relatively stress tolerant) and BGM2026 (susceptible) were tested against two water regimes such as water stress and non-stress. Results indicated that due to drought stress there was an increase in proline content and soluble sugar content and decrease in chlorophyll a content, chlorophyll b content, total chlorophyll content, chlorophyll a/b ratio and cell membrane stability. Proline and soluble sugar showed more content in tolerant genotype than in susceptible ones. Chlorophyll reduction was most significant and cell membrane stability was found minimal in susceptible genotypes. From the result, genotype BGM2026 which recorded the lowest proline, soluble sugar content and highest chlorophyll reduction and cell membrane injury was considered as drought susceptible. The variety/genotype of soybean such as BARI Soybean-6, Shohag and BD2331 were more drought stress tolerant and better mechanisms of drought tolerance.Bangladesh J. Agril. Res. 42(3): 475-485, September 2017


2014 ◽  
Vol 58 (1) ◽  
pp. 64-70 ◽  
Author(s):  
M. S. Saleh ◽  
A. A. Al-Doss ◽  
A. A. Elshafei ◽  
K. A. Moustafa ◽  
F. H. Al-Qurainy ◽  
...  

2020 ◽  
Vol 12 (3) ◽  
pp. 216-220
Author(s):  
R. Chipilski ◽  
B. Kyosev ◽  
R. Cholakova

Abstract. The objective of this study was to investigate the reaction processes of seedlings originating from wheat seeds after they were subjected to accelerated ageing, which imitated low-temperature storage. Germination, vigor, morphological characteristics, relative chlorophyll content and antioxidant enzyme activity were measured in seedlings and young plants of wheat (Triticum aestivum L.) after processes of accelerated ageing of the seeds. The ageing procedures of the seeds were done according to the standard conditions (40±0.5°C and 100% air relative humidity) of the International Seed Testing Association for 72 and 120 hours of treatments of the Bulgarian varieties Geya-1 and Sadovo 772. For the control were used seeds, which were not subjected to the accelerated ageing conditions. The coleoptile of 5 days seedlings and second leaf of 10 and 15 days plants in seedling stage, following the seed accelerated ageing procedure, was measured in the laboratory and the greenhouse. The seed germination rate and vigor, fresh and dry weight of growing coleoptiles were inhibited after different periods of ageing exposure and well correlated with increased accumulation of total hydrogen peroxide, malondialdehyde content, guaiacol peroxidase activity and rate of cells membrane stability index. The negative changes of chlorophyll content index, fresh and dry weight and leaf area of the leaves of young plants descending from aged seeds sowed in pots were found. It was established that the modern variety Geya-1 was more tolerant to the applied ageing condition than the older Sadovo 772. In conclusion, we considered that accelerated ageing could be used as a model for estimation of seed deterioration in wheat after long-term storage.


2018 ◽  
Vol 16 (1) ◽  
pp. 1-6 ◽  
Author(s):  
I Nowsherwan ◽  
G Shabbir ◽  
SI Malik ◽  
M Ilyas ◽  
MS Iqbal ◽  
...  

The present study was designed to evaluate the changes in different physiological traits such as proline content, cell membrane stability, relative water content and chlorophyll content under drought stress in sixteen wheat genotypes. Wheat genotypes (99FJ-03, Marvi-2000, WC- 13, WC-24, WC-19, Faisalabad-85, Kaghan, Bahawalpur, Zarlashta, Punjab-96, Shafaq, Maxi-pak, WC-20, Chenab-70, AUR-0809, Chakwal) were sown during rabiseason of 2013-14 following randomized complete block design with three replications. Drought stress was induced by withholding water for 30 days at heading and anthesis stage. Genotypes were significant for different physiological traits like relative water content, proline content, cell membrane stability and chlorophyll content under drought stress which indicated that some genotypes were more tolerant against drought stress than others. Among tested wheat genotypes, Maxi-Pak was found to be potential variety for relative water content, cell membrane stability, chlorophyll content and yield. Hence, it can be used in future wheat breeding programme for developing drought tolerant genotypes.SAARC J. Agri., 16(1): 1-6 (2018)


1988 ◽  
Vol 68 (4) ◽  
pp. 935-940 ◽  
Author(s):  
M. TOLLENAAR ◽  
T. W. BRUULSEMA

The response of rate and duration of kernel dry matter accumulation to temperatures in the range 10–25 °C was studied for two maize (Zea mays L.) hybrids grown under controlled-environment conditions. Kernel growth rates during the period of linear kernel growth increased linearly with temperature (b = 0.3 mg kernel−1 d−1 °C−1). Kernel dry weight at physiological maturity varied little among temperature treatments because the increase in kernel growth rate with increase in temperature was associated with a decline in the duration of kernel growth proportional to the increase in kernel growth rate.Key words: Zea mays L, period of linear kernel dry matter accumulation, controlled-environment conditions, kernel growth rate


2015 ◽  
Vol 67 (3) ◽  
pp. 993-1000 ◽  
Author(s):  
Lydia Shtereva ◽  
Roumiana Vassilevska-Ivanova ◽  
Tanya Karceva

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.


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