scholarly journals DEPENDENCE BETWEEN THE ANTIMICROBIAL ACTIVITY AND THE MOLECULAR STRUCTURE OF PHENOLIC ACIDS AND THEIR SALTS WITH ALKALI METALS AS BIOLOGICALLY ACTIVE SUBSTANCES PRESENT IN THE NATURAL PRODUCTS

2020 ◽  
pp. 25-36
Author(s):  
Jolanta Piekut ◽  
Małgorzata Kowczyk-Sadowy ◽  
Małgorzata Krasowska
Keyword(s):  
Molecular Structure ◽  
Antimicrobial Activity ◽  
Natural Products ◽  
Phenolic Acids ◽  
Alkali Metals ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Substances

scholarly journals Opportunities for using biologically active substances Rhodiola rosea L. in the production of functional food with consideration for antimicrobial activity

2020 ◽  
Vol 176 ◽  
pp. 01011
Author(s):  
Alexandra Zaushintsena ◽  
Olga Babich ◽  
Evgeny Bryukhachev ◽  
Irina Milenteva ◽  
Anton Starcev
Keyword(s):  
Antimicrobial Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Growth Zone ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Diffusion Method ◽  
Antibiotic Effect ◽  
Active Substances

Biologically active substances extricated by extraction of water and 40% ethyl alcohol from rootstalks of Rhodiola rosea L.: gallic acid, forms of rosovin and salidroside have been studied. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used during the research. Determination of antimicrobial activity was performed by agar diffusion method (OFC.1.2.4.0010.15) [8]. The method is based on the evaluation of inhibition of the growth zone of testing cultures of microorganisms by certain concentrations of biologically active substances (BAS). String formed bacteria (8 strains), cocci bacteria (3) and fungal (4) microorganisms, bifidobacterium (10 strains) were used as testing cultures. Various responses of microorganisms to exposure by biologically active substances have been determined. Gallic acid and salidroside in the aqueous phase have the maximum inhibitory property to rod bacteria and coccoid microorganisms. Post-antibiotic effect is shown in rozovin. Salidroside in the butanol phase is more neutral to most microorganisms, with the exclusion of: Acinetobacter baumannii, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus. The lack of a clearly expressed reaction of BAS to bifidobacterium, most probably, gives the possibility of their use in the development of functional products of a new generation. However, first of all, it is required to organize a special experiment on the structure of the introduction of the studied components into the lactoserum. Next, determine the content of BAS in the derived product, the reaction of bifidobacterium admitted for use in biotechnology, and a standard set of microbiological strains. Gallic acid is suggested to be introduced in applied studies to explore as a target immunomodulator of resistance to the listed microflora and to develop an effective form of a new product.

scholarly journals ASSESSMENT OF THE PROSPECTS OF STUDYING AND USING MUSHROOMS OF AZERBAIJAN AS EFFECTIVE PRODUCERS OF BIOLOGICALLY ACTIVE SUBSTANCES

2020 ◽  
Vol 17 (34) ◽  
pp. 403-411
Author(s):  
Konul Farrukh BAKHSHALIYEVA ◽  
Nizami Rza NAMAZOV ◽  
Arzu Rasul HASANOVA ◽  
Fidan Rasim MAMMADOVA ◽  
Panah Zulfigar MURADOV
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Ganoderma Lucidum ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Culture Solution ◽  
Relationship Of ◽  
The Relationship ◽  
Active Substances ◽  
Biological Active Substances

In the carried out of the research, Xylotroph macromycetes have been researched as a perspective producer of bıologıcal actıve substances used for various purposes today in world practice which spread in Azerbaijan and some of their features (the biological activity of synthesized metabolites, toxicity, antimicrobial activity and dynamics of biomass formation ). It became clear that fungi such as Ganoderma lucidum (Curtis) P. Karst., Laetiporus sulphureus (Bull.) Murrill, Pleurotus ostreatus (Jacq.) P. Kumm, Schyzophyllum commune Fr and Trametes versicolor (L.) Lloyd considered as a perspective producer of bıologıcal actıve substances is widely spread in the forests of Azerbaijan, and some of them are even dominant species of xylomicobiot inherent in the nature of Azerbaijan. As a result of studies with isolated strains of these fungi, it was shown that both in the culture solution (CS) and in the vegetative mycelia (VB), there are metabolites with biological activity. The result of research also became clear that antimicrobial activity of CS in all cases is higher than in water or alcohol extracts of dry biomass (1,1-1,2 times), although it defines the overall biological activity of VB extracts to the relationship of Paramecium caudatum gives a higher increase effect. In addition, the selected active strains do not lag behind the known strains in terms of the amount of biomass formed (up to 8.7 g/l in 7 days) and manifestations of forms of biological activity. This gives a serious basis to realize the production of biologically active substances for different (food, feed, medical and other) purposes on their basis.

scholarly journals The Content of Secondary Metabolites and Antioxidant Activity of Wild Strawberry Fruit (Fragaria vescaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Magdalena Dyduch-Siemińska ◽  
Agnieszka Najda ◽  
Jan Dyduch ◽  
Magdalena Gantner ◽  
Kamila Klimek
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Strawberry Fruit ◽  
Dpph Radical ◽  
Dried Fruits ◽  
Wild Strawberry ◽  
Neutralization Ability ◽  
Active Substances

Chemical analyses carried out in 2011–2013 aimed at evaluating the contents of flavonoids, free phenolic acids, tannins, anthocyanins, and antioxidant activity (%) by means of DPPH radical neutralization ability in fresh and air-dried fruits of three wild strawberry cultivars. Examinations revealed differences in contents of biologically active substances determined in raw versus dried material depending on the cultivar. Mean concentrations of flavonoids and tannins were highest in raw fruits of “Baron von Solemacher” cv., which amounted to 1.244 mg·g−1and 6.09%, respectively. Fresh fruits of “Regina” cv. were characterized by the highest average content of phenolic acids and anthocyanins: 4.987 mg·g−1and 0.636 mg·100 g−1. The pattern of mean contents of biologically active substances analyzed in air-dried fruits was similar. Significant differences in abilities to neutralize the DPPH radical to diphenylpicrylhydrazine by extracts made of examined wild strawberry fruits were also indicated.

scholarly journals Quantitative analysis of biologically active substances and the investigation of antioxidant and antimicrobial activities of some extracts of Osage orange fruits

Pharmacia ◽  
2021 ◽  
Vol 68 (4) ◽  
pp. 731-739
Author(s):  
Ani Slavik Dadayan ◽  
Lala Ashot Stepanyan ◽  
Tatevik Hovhannes Sargsyan ◽  
Anahit Mkhitar Hovhannisyan ◽  
Slavik Arshak Dadayan
Keyword(s):  
Antimicrobial Activity ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Osage Orange ◽  
Wide Range ◽  
Pharmacologically Active ◽  
Active Substances

Biologically active substances, the antioxidant and antimicrobial activity of aqueous, 70% ethylate and ethyl acetate extracts of fruits of maclura orange (Osage Orange), a representative of the Moraceae genus growing in the Ararat region of Armenia have been studied. It is found that plant extracts are rich in a wide range of pharmacologically active substances, in particular, tannins, flavonoids, organic acids, vitamins, anthocyanins, micro- and microelements, etc. Based on the investigations performed, extracts of maclura orange can be recommended as a source of biologically active substances (BAS), as well as a preventive and corrective agent in deficiency or imbalance of macro- and microelements in tissue and cellular structures in various pathological processes in the body. An ethanol extract of maclura fruits exhibits the antimicrobial activity against Bacillus subtilis 1820, E. Coli 5002, Serratia marcescens 5251 and Staphylococcus aureus ATCC-6538 strains. Maclura extracts can be used as an environmentally friendly source of antioxidants against early aging.

scholarly journals Innovative Method for Obtaining Biologically Active Compounds from Brewery Mash

2021 ◽  
Vol 51 (1) ◽  
pp. 113-124
Author(s):  
Konstantin Kobelev ◽  
Marina Gernet ◽  
Irina Gribkova
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Raw Materials ◽  
Ethanol Solution ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Cellulolytic Enzyme ◽  
Enzyme Preparations ◽  
Naoh Solution ◽  
Active Substances

Introduction. Brewery mash, or brewer’s spent grain (BSG), is a by-product of brewing industry. It is known to contain valuable biologically active substances. However, their extraction is complicated by the presence of various polymers. The research featured various physicochemical methods for obtaining valuable biological compounds from brewery waste. The new method modified complex non-starch polysaccharides, lignin, arabinoxylans, and other high-molecular compounds associated with phenolic compounds. The research objective was to solve the problem of recycling industrial by-products that accumulate in large quantities and require expensive processing or disposal. The paper introduces new technological approaches for deep processing of BSG as a source of secondary raw materials in order to obtain extracts fortified with polyphenolic compounds. Study objects and methods. The research featured BSG from malt subjected to treatment with ECA-activated water (catholyte with pH 9.6 ± 0.1), followed by enzymatic hydrolysis of cellulolytic enzyme preparations and extraction with a polar solvent of the resulting free polyphenolic substances. The experiments were based on standard methods for assessing the content of various biologically active substances. Results and discussion. A 70% water-ethanol solution proved to be optimal at the BSG:extractant ratio of 2:1, process temperature = 50 ± 2°C, and extraction time = 60 ± 5 min. Under the same conditions, 70 %vol. of beer distillate made it possible to extract phenolic acids, flavonoid rutin, irreplaceable and nonessential amino acids, and non-starch polysaccharide β-glucan from the BSG matrix. The BSG treatment with 1M NaOH solution delivered viscous hydrolysates fortified with flavonoids rutin and quercetin, which did not happen when acid hydrolysis was used. The combined use of ECA-treated water (catholyte with pH 9.6 ± 0.1) for 24 ± 0.05 h, combined with biocatalysis with the enzyme preparation Viskoflo MG for 2 ± 0.05 h, made it possible to obtain BSG extracts with a high content of phenolic acids and aldehydes, as well as flavonoid rutin. Conclusion. The study revealed the mechanism of hydrolytic decomposition of BSG non-starch polysaccharides, considering the compounds contained in the extracts. The BSG hydrolysates fortified with various phenolic compounds can be used in various food technologies, e.g., in fermented drinks.

Antimicrobial activity of water-alcohol extracts of Hedysarum neglectum

2020 ◽  
Author(s):  
L.S. Dyshlyuk L.S. ◽  
◽  
N.V. Fotina N.V.
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Alcohol ◽  
Aqueous Ethanol ◽  
Maximum Activity ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Ethanol Extracts ◽  
Promising Source ◽  
Active Substances ◽  
Water Alcohol

Hedysarum neglectum is a promising source of biologically active substances that exhibit antimicrobial activity. We determined the antimicrobial activity of aqueous ethanol extracts of Hedysarum neglectum. The maximum activity is observed in the extract obtained at a temperature of 50 °C using 50 % ethyl alcohol.

Microscopic fungi of lithobiont communities of Argentine Islands Region: Data from the 22nd Ukrainian Antarctic Expedition

2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell
Keyword(s):  
Filamentous Fungi ◽  
Mucor Circinelloides ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Microscopic Fungi ◽  
Rhodotorula Rubra ◽  
Yeast Fungi ◽  
Antarctic Expedition ◽  
Geomyces Pannorum ◽  
Active Substances

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.

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