scholarly journals Incidence of micronuclei in pregnant women and cord blood samples before and after the bombing of Serbia

2004 ◽  
Vol 12 (4) ◽  
pp. 200-202 ◽  
Author(s):  
Miroslava Stankovic ◽  
Gordana Joksic ◽  
Marija Guc-Scekic
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Calf Serum ◽  
Study Groups ◽  
Peripheral Blood Lymphocytes ◽  
Blood Samples ◽  
Blood Lymphocytes ◽  
Before And After ◽  
Other Substances ◽  
Fetal Cord Blood

BACKGROUND: This study provides the data regarding monitoring of population using CB-micronuclei assay in the period 1995-2001 in Serbia. The target groups consisted of 45 pregnant women of mean age 34.3(6.56) years, unaware of being exposed to chemicals drugs or other substances and undergoing cordocentesis. The incidence of micronuclei (MN) in peripheral blood lymphocytes and in fetal cord blood lymphocytes was analyzed. METHODS: The study was carried out on cultures of PHA-stimulated blood lymphocytes. Three drops of blood samples ware added into 5ml RPMI-1640 (Gibco) medium supplemented with 15% of calf serum and PHA (Gibco, 2.5?g/ml). For micronuclei preparation the cytokinesis block method was used (Fenech et al., 1993). RESULTS: The results of the study showed that in the year 1995, the incidence of micronuclei in pregnant women was 9.61(3.26) per 1000 binucleated (BN) cells, and 3.74(1.60) in cord blood samples per 1000 BN cells, respectively. In 2000 the incidence of micronuclei in study group was 28.26(7.87) per 1000 BN cells, and in cord blood samples 22.22(5.63) per 1000 BN cells. One year later (2001) the incidence of micronuclei in pregnant woman slightly decreased and reached the value of 26.98(4.50), while in cord blood it slightly increased up to 26.58(6.85) per 1000 BN cells. CONCLUSION: The monitoring data obtained in this study have shown significantly increase of micronuclei (2- to 3-fold) in study groups in 2000 and 2001.

scholarly journals Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

2008 ◽  
Vol 215 (4) ◽  
pp. 363-371 ◽  
Author(s):  
Darko Grujicic ◽  
Olivera Miloševic-Djordjevic ◽  
Slobodan Arsenijevic ◽  
Dragoslav Marinkovic
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Lymphocytes ◽  
Cord Blood Lymphocytes

scholarly journals CD81 expression in peripheral blood lymphocytes before and after treatment with interferon and ribavirin in HIV/HCV coinfected patients

HIV Medicine ◽  
2010 ◽  
Vol 11 (3) ◽  
pp. 161-169 ◽  
Author(s):  
D Micheloud ◽  
J González-Nicolás ◽  
J Berenguer ◽  
R Lorente ◽  
P Miralles ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Before And After

scholarly journals Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Maria García-Ricobaraza ◽  
Mercedes García-Bermúdez ◽  
Francisco J. Torres-Espinola ◽  
M. Teresa Segura Moreno ◽  
Mathieu N. Bleyere ◽  
...  
Keyword(s):  
Immune System ◽  
Gene Polymorphism ◽  
Cord Blood ◽  
Pregnant Women ◽  
Venous Blood ◽  
Innate Immune ◽  
Peroxisome Proliferator ◽  
Blood Samples ◽  
Inflammatory Reactions ◽  
Immune Parameters

Abstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of monocytes/macrophages and the control of inflammatory reactions. The aim of this study was to evaluate the relationship between the Pro12Ala (rs1808212) PPARG gene polymorphism on immune molecular and cellular components in mothers and their offspring participating in the PREOBE study. Methods DNA from maternal venous blood samples at 24, 34 and 40 gestational weeks, plus cord blood samples was extracted. Pro12Ala PPARG polymorphism genotyping was performed, and immune system markers were analyzed by flow cytometry. Results Study findings revealed no effect of rs1808212 PPARG genotypes on innate immune parameters in mothers and their offspring; however, CD4 + /CD8 + ratio were decreased at 24 and 34 weeks in pregnant women carrying the CG (Pro12Ala) rs1808212 polymorphism, (p = 0,012 and p = 0,030; respectively). Only CD19 levels in peripheral blood were significantly higher at delivery in pregnant women carrying the CC (Pro12Pro) genotype (p ≤ 0.001). Moreover, there were statistically significant differences in leukocytes and neutrophils maternal levels at 34 weeks of gestation, being lower in carriers of Pro12Ala genotype (p = 0.028 and p = 0.031, respectively). Conclusions Results suggest that Pro12Ala PPARG polymorphism may have an effect on some cell and immune parameters in pregnant women during pregnancy and at time of delivery. However, newborn innate immune system does not seems to be influenced by PPARG Pro12Ala polymorphism in cord blood.

scholarly journals A Case Report to Assess Passive Immunity in a COVID Positive Pregnant Patient

2020 ◽  
Vol 37 (12) ◽  
pp. 1280-1282
Author(s):  
Lorraine E. Toner ◽  
Shari E. Gelber ◽  
Juan A. Pena ◽  
Nathan S. Fox ◽  
Andrei Rebarber
Keyword(s):  
Case Report ◽  
Cord Blood ◽  
Pregnant Patient ◽  
Passive Immunity ◽  
Molecular Testing ◽  
Transplacental Passage ◽  
Blood Samples ◽  
Key Points ◽  
Fetal Cord Blood ◽  
Red Blood Cell Isoimmunization

Introduction Data regarding transplacental passage of maternal coronavirus disease 2019 (COVID-19) antibodies and potential immunity in the newborn is limited. Case Report We present a 25-year-old multigravida with known red blood cell isoimmunization, who was found to be COVID-19 positive at 27 weeks of gestation while undergoing serial periumbilical blood sampling and intrauterine transfusions. Maternal COVID-19 antibody was detected 2 weeks after positive molecular testing. Antibodies were never detected on cord blood samples from two intrauterine fetal cord blood samples as well as neonatal cord blood at the time of delivery. Conclusion This case demonstrates a lack of passive immunity of COVID-19 antibodies from a positive pregnant woman to her fetus, neither in utero nor at the time of birth. Further studies are needed to understand if passage of antibodies can occur and if that can confer passive immunity in the newborn. Key Points

Intracellular Cytokine Profile of Cord and Adult Blood Lymphocytes

Blood ◽  
1998 ◽  
Vol 92 (1) ◽  
pp. 11-18 ◽  
Author(s):  
I.M.H. Chalmers ◽  
G. Janossy ◽  
M. Contreras ◽  
C. Navarrete
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Cytokine Profile ◽  
Color Flow ◽  
Blood Lymphocytes ◽  
Tnf Α ◽  
Ifn Γ

Umbilical cord blood (CB) transplantation is thought to be associated with a reduced risk of severe graft-versus-host-disease (GVHD) compared with bone marrow transplantation (BMT). The cytokine cascade is known to be important in the pathogenesis of GVHD; however, previous studies investigating the cytokine secretion pattern of CB cells have been contradictory because of variations in experimental techniques. In this study, the cytokine profile of cord and adult blood lymphocytes and lymphocyte subsets has been assessed at the single-cell level by flow cytometry, using CD4/CD8 and CD45RA/CD45RO markers. Cord and adult blood mononuclear cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin in the presence of monensin. After 4 to 24 hours of incubation, interleukin-2 (IL-2), IL-4, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) production was measured by three-color flow cytometry. The results show that cord blood lymphocytes (CBL) produce less IL-2, IL-4, IFN-γ, and TNF-α than adult peripheral blood lymphocytes (ABL). Further subset analysis showed that in CBL the majority of cytokine producing cells were CD4+CD45RA+, whereas in ABL the cytokine-producing cells were both CD4+CD45RO+ and CD8+CD45RO+. These results suggest that the reduced incidence of GVHD in CB transplantation may partly due to the altered cytokine profile seen in CBL.

scholarly journals Antibodies to Pneumococcal Proteins PhtD, CbpA, and LytC in Filipino Pregnant Women and Their Infants in Relation to Pneumococcal Carriage

2009 ◽  
Vol 16 (6) ◽  
pp. 916-923 ◽  
Author(s):  
Emma Holmlund ◽  
Beatriz Quiambao ◽  
Jukka Ollgren ◽  
Teija Jaakkola ◽  
Cécile Neyt ◽  
...  
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Vaccine Candidate ◽  
Geometric Mean ◽  
Protein A ◽  
Nasopharyngeal Swab ◽  
Serum Samples ◽  
Blood Samples ◽  
Clear Cut ◽  
Pneumococcal Carriage

ABSTRACTThis study focuses on the immunogenicity of the following three pneumococcal vaccine candidate proteins in Filipino infants, all inducing protection in animal models: pneumococcal histidine triad protein D (PhtD), choline binding protein A (CbpA), and the lysozyme LytC. The immunoglobulin G antibody concentrations to PhtD, its putative, protective, and exposed C-terminal fragment (PhtD C), CbpA, and LytC were measured by enzyme immunoassay in 52 serum samples from pregnant women, 39 cord blood samples, and consecutive serum samples (n= 263) from 52 newborns between 6 weeks and 10 months of age scheduled to be taken at six time points. A nasopharyngeal swab to detect pneumococcal carriage was taken parallel to the serum samples. The antibody concentrations in the cord blood samples were similar to those in the samples from the mothers. In infant sera, the geometric mean antibody concentrations (GMCs) for all three proteins decreased until the age of 18 weeks and started to increase after that age, suggesting that the infants' own antibody production started close to the age of 4 to 5 months. The increase in GMCs by age, most clear-cut for CbpA, was associated with pneumococcal carriage. Anti-PhtD concentrations were higher than anti-PhtD C concentrations but correlated well (rof 0.89 at 10.5 months), suggesting that antibodies are directed to the supposedly exposed and protective C-terminal part of PhtD. Our results show that young children are able to develop an antibody response to PhtD, CbpA, and LytC and encourage the development of pneumococcal protein vaccines for this age group.

scholarly journals Impact of antenatal counselling on couples’ knowledge and practice of contraception in Mansoura, Egypt

1999 ◽  
Vol 5 (5) ◽  
pp. 1002-1013
Author(s):  
M. H. Soliman
Keyword(s):  
Family Planning ◽  
Antenatal Care ◽  
Pregnant Women ◽  
Study Groups ◽  
Study Group ◽  
Before And After ◽  
Joint Decisions ◽  
Knowledge And Practice ◽  
The Impact ◽  
Interview Questionnaire

The impact of antenatal counselling on couples’ knowledge and practice of contraception was investigated. An interview questionnaire was used before and after conducting counselling sessions with 200 pregnant women and 100 spouses. The participants were followed up immediately after delivery and 3 months later. Both the control and study groups displayed a lack of knowledge of contraception. Counselling sessions improved the couples’ knowledge and practice in the study group. Involving husbands in family planning counselling sessions led to joint decisions being made and encouraged women’s use of contraception. The majority of couples retained most of the information given. Integrating family planning counselling into antenatal care in all facilities and involving the husband are recommended

scholarly journals FEATURES OF LYMPHOCYTES IN THE BLOOD IN PATIENTS WITH BASAL-LIKE BREAST CANCER SOUTH-EAST UKRAINE

2016 ◽  
Author(s):  
T. Yu. Pohorila ◽  
N. F. Shchurov ◽  
S. M. Pashchenko
Keyword(s):  
Breast Cancer ◽  
Peripheral Blood ◽  
Antitumor Immunity ◽  
Morphological Characteristics ◽  
Peripheral Blood Lymphocytes ◽  
Comprehensive Treatment ◽  
Breast Cancer Patients ◽  
Blood Lymphocytes ◽  
Before And After ◽  
Basal Like Breast Cancer

Lymphocytes are important in antitumor immunity. Therefore, the estimate of the magnitude of lymphocytes, could be used to determine the protective capabilities of the organism basal-like breast cancer patients. The purpose of the study: building a morphological features peripheral blood lymphocytes of patients with basal-like breast cancer South-East Ukraine, receiving comprehensive chemoradiotherapy. It was found that treatment BLT changes the structure of peripheral blood lymphocytes. After the comprehensive treatment of peripheral blood lymphocytes of patients significantly increased in size, they decreased nuclear-cytoplasmic ratio. For definitions immune status of patients basal-like breast cancer no small role played by morphological characteristics of lymphocytes in the peripheral blood before and after complex treatment.

scholarly journals P2 Receptor Subtypes in Human Hematopoietic Cells of Peripheral and Cord Blood

2020 ◽  
Vol 11 (1) ◽  
pp. 8121-8128
Keyword(s):  
Cord Blood ◽  
Peripheral Blood ◽  
P2y Receptors ◽  
P2x Receptors ◽  
Peripheral Blood Lymphocytes ◽  
Receptor Subtypes ◽  
Blood Monocytes ◽  
P2x7 Receptors ◽  
Blood Lymphocytes ◽  
Significant Difference

P2 receptors have been found in several blood cells and their progenitors. However, most studies lack data about receptor subtypes and of receptors expression time in the process of cell differentiation. The aim of our study was to identify the subtypes of P2Y and P2X receptors on human CD34+ cells, c-kit+ cells, monocytes, lymphocytes of cord and peripheral blood. Expression of P2Y1, P2Y4, and P2Y6 receptors was the uniform in the cord and peripheral blood of all studied cells with the prevalence of monocytes expressing P2Y-receptors (up to 71%). At the same time, a significant difference was found between cells of cord and peripheral blood expressing subtypes of P2X2, P2X3, P2X4, P2X5, P2X6, P2X7 receptors. Cord blood lymphocytes contained a higher percentage of P2X receptors than peripheral blood lymphocytes. Similarly, the percentage of the peripheral blood monocytes, containing P2X receptors was significantly higher than the monocytes of cord blood.

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