scholarly journals Influence of steeping time on biological activity of black mulberry leaves tea

2016 ◽  
pp. 177-191 ◽  
Author(s):  
Sladjana Cestic ◽  
Marija Radojkovic ◽  
Aleksandra Cvetanovic ◽  
Pavle Maskovic ◽  
Sasa Djurovic
Keyword(s):  
Total Phenolics ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Mulberry Leaves ◽  
Tea Quality ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Polyphenolic Profile ◽  
Boiled Water

Black mulberry leaves teas (BMLTs) were prepared using boiled water and different steeping time (5 10, 20, 35 and 45 minutes). In order to establish the connection between steeping time and tea quality, total phenolics content (TPC), total flavonoids content TFC contents were measured, as well as two antioxidant assays (DPPH and reducing power assays), alone with antimicrobial and cytotoxic tests. The obtained results showed that TPC, TFC, IC50 and EC50 values increased with the increase in steeping time, while antimicrobial and cytotoxic activity exhibited different tendency. Based on the obtained results, the 10-minute steeping time was the optimum for tea preparation and this tea was used for the determination of polyphenolic profile using HPLC-MS technique. The results showed that the main compounds in BMLT were chlorogenic and caffeic acids with the contents of 7226.00 and 537.52 ?g/g, respectively.

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

scholarly journals Reverse Phase HPLC Profiling of Antioxidant and Antimicrobial Activities of Sarcochlamys pulcherrima (Roxb.) Gaud.

2016 ◽  
Vol 5 (6) ◽  
pp. 49-54
Author(s):  
Pradip Doley ◽  
Naorem Manglembi Devi ◽  
Ch. B. Singh ◽  
A. V. Singh
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Reducing Power ◽  
Reverse Phase ◽  
Butanol Fraction ◽  
Total Flavonoids Content ◽  
Phenolics Content

The total phenolics content, total flavonoids content, antioxidant activity and antimicrobial activity of methanol extract and its derived fractions from Sarcochlamys pulcherrima (Roxb.) Gaud. were evaluated. The plant material was initially extracted with methanol. The fractionation was carried out using liquid-liquid extraction with hexane, ethyl acetate, n-butanol, and water. The Folin-Ciocalteu assay was used to quantify total phenolics content, Aluminum Chloride assay to quantify total flavonoids content, DPPH (2, 2-diphenyl-1-picrylhydrazine) and reducing power assay are to determine antioxidant activity. The ethyl acetate fraction was observed to contain high phenolic content (201.37 mg/g) followed by n-butanol fraction (197.76 mg/g), methanol extract (136.93mg), water fraction (55.05mg/g) and hexane fraction (36.94mg/g) respectively. The flavonoids content ranges from 109.48 mg/g to 8.63 mg/g dry extract. The highest antioxidant activity was found in n-butanol fraction (EC50 =14.69 µg/mL in DPPH and 162.65 µg/mL in reducing power). The antimicrobial activity of the samples was evaluated based on inhibition zone using agar-well diffusion assay and minimal inhibition concentration (MIC) values. Hexane fraction had no antimicrobial effect against test microorganisms. The zone of inhibitions ranged from 11 mm to 6 mm. The n-butanol fraction possessed the highest antimicrobial activity against E. coli among the four fractions with MIC value of 0.781mg/mL. In addition rutin, gallic acid, quercetin and kaempferol were quantified by reverse phase high performance liquid chromatography (HPLC) method. In ethyl acetate and n-butanol fraction, observed large amount rutin (77.53 ± 7.57 mg/g; 77.45 ± 1.71 mg/g) and gallic acid (61.01 ± 2.34 mg/g; 22.75 ± 1.54 mg/g) as compared to the quercetin and kaempferol.Doley et al., International Current Pharmaceutical Journal, May 2016, 5(6): 49-54

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

scholarly journals Dietary intake of specific phenolic compounds and their effect on the antioxidant activity of daily food rations

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Wojciech Koch ◽  
Tomasz Baj ◽  
Wirginia Kukula-Koch ◽  
Zbigniew Marzec
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Female Students ◽  
Male And Female ◽  
Daily Food ◽  
Phenolic Components ◽  
Phenolics Content ◽  
Test Correlation

AbstractThe determination of phenolic acids’ and flavonoids’ content in daily food rations (DFRs) of a selected group of male and female students and the development of chromatographic conditions is the primary goal of the study. The presence of 7 phenolic components were confirmed in the prepared extracts from all diets reconstructed within a period of 3 years. The highest concentrations were determined for hesperidin (124 and 55.6 mg for women and men, respectively), naringin (47.6 mg in female and 37 mg in male diets) and chlorogenic acid (19.7 and 19.8 mg for women and men). The antioxidant potential of the daily food rations, measured with a DPPH test, was higher for women (range 47.1‒78.8%) than for men (range 34.5‒78.0%) and was found to strongly correlate with the total phenolics content of the samples (Folin-Ciocalteu test) (correlation coefficient 0.90).

scholarly journals RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

2016 ◽  
Vol 8 (12) ◽  
pp. 236 ◽  
Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Total Flavonoids ◽  
Wide Range ◽  
Phenolics Content

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>

scholarly journals The Effect of Salicylic Acid on Response to Water Deficit in Basil

2018 ◽  
Vol 2 (1) ◽  
pp. 42
Author(s):  
Adisa Paric ◽  
Erna Karalija
Keyword(s):  
Salicylic Acid ◽  
Photosynthetic Pigments ◽  
Peroxidase Activity ◽  
Total Phenolics ◽  
Stem Length ◽  
Limiting Factor ◽  
Total Flavonoids ◽  
Induced Stress ◽  
Total Flavonoids Content ◽  
Response To Water

Drought-induced stress is the main limiting factor that affects growth and development in plants. In this study, the effect of exogenous application of salicylic acid (SA) on drought tolerance in basil was investigated. The analysis showed that application of SA under drought stress had significant effects on physiological and biochemical parameters, such as photosynthetic pigments content, total phenolics, flavonoids, flavanols and protein content and peroxidise activity, but had no significant effects on the morphological parameters, such as stem length, length and areaof leaves . In drought conditions, total phenolics and peroxidase activity reduced significantly, but all photosynthetic pigments, total flavonoids, flavanols and proteins increased significantly. Application of SA displayed some alleviating effects against drought induced stress through increase of plant growth, total flavonoids content and peroxidase activity

scholarly journals Hydrogen Peroxide Treatment and the Phenylpropanoid Pathway Precursors Feeding Improve Phenolics and Antioxidant Capacity of Quinoa Sprouts via an Induction of L-Tyrosine and L-Phenylalanine Ammonia-Lyases Activities

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Michał Świeca
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Capacity ◽  
Total Phenolics ◽  
Shikimic Acid ◽  
Reducing Power ◽  
Phenylpropanoid Pathway ◽  
Germination Time ◽  
Pal Activity ◽  
Hydrogen Peroxide Treatment ◽  
Phenolics Content

Hydrogen peroxide treatment and the phenylpropanoid pathway precursors feeding affected the antioxidant capacity of quinoa sprouts. Compared to the control, total phenolics content was significantly increased by treatment of control sprouts with 50 mM and 200 mM H2O2—an elevation of about 24% and 28%, respectively. The highest increase of flavonoids content was found for the sprouts treated with 200 mM H2O2obtained from seeds fed with shikimic acid. All the studied modifications increased the antioxidant potential of sprouts (at least by 50% compared to control). The highest reducing power was found for the sprouts treated with 200 mM H2O2obtained by phenylalanine feeding (5.03 mg TE/g DW) and those obtained from the seeds fed with tyrosine (5.26 mg TE/g DW). The activities of L-tyrosine (TAL) and L-phenylalanine (PAL) ammonia-lyases were strongly affected by germination time as well as the applied modification of sprouting. On the 3rd day the highest PAL activity was determined for both untreated and induced with 50 mM H2O2sprouts obtained by phenylalanine feeding. H2O2induced TAL activity; the highest TAL activity was determined for 3-day-old sprouts induced with 200 mM H2O2obtained from seeds fed with phenylalanine.

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

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