scholarly journals Reverse Phase HPLC Profiling of Antioxidant and Antimicrobial Activities of Sarcochlamys pulcherrima (Roxb.) Gaud.

2016 ◽  
Vol 5 (6) ◽  
pp. 49-54
Author(s):  
Pradip Doley ◽  
Naorem Manglembi Devi ◽  
Ch. B. Singh ◽  
A. V. Singh
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Reducing Power ◽  
Reverse Phase ◽  
Butanol Fraction ◽  
Total Flavonoids Content ◽  
Phenolics Content

The total phenolics content, total flavonoids content, antioxidant activity and antimicrobial activity of methanol extract and its derived fractions from Sarcochlamys pulcherrima (Roxb.) Gaud. were evaluated. The plant material was initially extracted with methanol. The fractionation was carried out using liquid-liquid extraction with hexane, ethyl acetate, n-butanol, and water. The Folin-Ciocalteu assay was used to quantify total phenolics content, Aluminum Chloride assay to quantify total flavonoids content, DPPH (2, 2-diphenyl-1-picrylhydrazine) and reducing power assay are to determine antioxidant activity. The ethyl acetate fraction was observed to contain high phenolic content (201.37 mg/g) followed by n-butanol fraction (197.76 mg/g), methanol extract (136.93mg), water fraction (55.05mg/g) and hexane fraction (36.94mg/g) respectively. The flavonoids content ranges from 109.48 mg/g to 8.63 mg/g dry extract. The highest antioxidant activity was found in n-butanol fraction (EC50 =14.69 µg/mL in DPPH and 162.65 µg/mL in reducing power). The antimicrobial activity of the samples was evaluated based on inhibition zone using agar-well diffusion assay and minimal inhibition concentration (MIC) values. Hexane fraction had no antimicrobial effect against test microorganisms. The zone of inhibitions ranged from 11 mm to 6 mm. The n-butanol fraction possessed the highest antimicrobial activity against E. coli among the four fractions with MIC value of 0.781mg/mL. In addition rutin, gallic acid, quercetin and kaempferol were quantified by reverse phase high performance liquid chromatography (HPLC) method. In ethyl acetate and n-butanol fraction, observed large amount rutin (77.53 ± 7.57 mg/g; 77.45 ± 1.71 mg/g) and gallic acid (61.01 ± 2.34 mg/g; 22.75 ± 1.54 mg/g) as compared to the quercetin and kaempferol.Doley et al., International Current Pharmaceutical Journal, May 2016, 5(6): 49-54

scholarly journals Influence of the Addition of Selected Spices on Sensory Quality and Biological Activity of Honey

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Aleksandra Wilczyńska ◽  
Joanna Newerli-Guz ◽  
Piotr Szweda
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Food Industry ◽  
Sensory Quality ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Positive Health ◽  
Functional Product ◽  
Phenolics Content ◽  
Growth Of Bacteria

Bee honey is nutritious and has numerous health benefits, but its taste is for many people too bland. Honey with addition of spices could be important to the food industry as a functional product with positive health image and interesting taste. Such product would definitely meet health-driven consumers’ expectations. Therefore, the aim of this study was to evaluate the effect of addition of selected spices on sensory, antimicrobial, and antioxidant properties of honey. Results showed that the addition of spices significantly affected the taste and the smell of honey (p<0,05) and that honey with the cinnamon was the most desired and easily accepted product by the consumers. The addition of spices had no significant effect on texture and appearance. All tested samples showed the ability to inhibit the growth of bacteria and, contrary to the assumptions, the addition of spices did not cause an increase of antimicrobial activity. The results also showed that the kind and amount of added spice significantly affected the antioxidant activity: ability to scavenge free radicals and total phenolics content. The highest antioxidant activity revealed the honey with cinnamon and the lowest revealed the honey with cardamom addition.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

scholarly journals Influence of steeping time on biological activity of black mulberry leaves tea

2016 ◽  
pp. 177-191 ◽  
Author(s):  
Sladjana Cestic ◽  
Marija Radojkovic ◽  
Aleksandra Cvetanovic ◽  
Pavle Maskovic ◽  
Sasa Djurovic
Keyword(s):  
Total Phenolics ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Mulberry Leaves ◽  
Tea Quality ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Polyphenolic Profile ◽  
Boiled Water

Black mulberry leaves teas (BMLTs) were prepared using boiled water and different steeping time (5 10, 20, 35 and 45 minutes). In order to establish the connection between steeping time and tea quality, total phenolics content (TPC), total flavonoids content TFC contents were measured, as well as two antioxidant assays (DPPH and reducing power assays), alone with antimicrobial and cytotoxic tests. The obtained results showed that TPC, TFC, IC50 and EC50 values increased with the increase in steeping time, while antimicrobial and cytotoxic activity exhibited different tendency. Based on the obtained results, the 10-minute steeping time was the optimum for tea preparation and this tea was used for the determination of polyphenolic profile using HPLC-MS technique. The results showed that the main compounds in BMLT were chlorogenic and caffeic acids with the contents of 7226.00 and 537.52 ?g/g, respectively.

QUANTIFICATION OF TOTAL PHENOLS AND ANTIOXIDANT ACTIVITY IN CLEMATIS ERECTA AERIAL PARTS

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (12) ◽  
pp. 52-54
Author(s):  
R. Chawla ◽  
◽  
D. Kumar ◽  
K Rana ◽  
H. Joshi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Total Phenols ◽  
Standard Drug ◽  
Butanol Fraction ◽  
Aerial Parts

The present investigation was planned to estimate total phenols and antioxidant activity in Clematis erecta aerial parts. Properly identified powdered plant was successively extracted in Soxhlet apparatus with solvents in order of increasing polarity viz., petroleum ether, chloroform, methanol and water. Ethyl acetate fraction and 1-butanol fraction of methanol extract were prepared using standardized procedure. Quantification of phenols was done on the basis of standard curve of gallic acid using standardized procedures. In vitro antioxidant activity was evaluated using DPPH assay in comparison to standard drug rutin. Maximum phenols content and antioxidant activity was found in ethyl acetate fraction followed by methanol extract and 1-butanol fraction, whereas negligible phenols content and antioxidant activity was observed in petroleum ether, chloroform and water extracts. The available literature reveals that polyphenols have been reported to possess antioxidant properties. Finally, it can be concluded that these polyphenols may be responsible for antioxidant properties.

Antioxidant Activity of Lichen Parmotrema austrosinense and its Antimicrobial Effect on Selected Plant Pathogens

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Shyam Kumar Rajaram ◽  
Ganesh Moorthy Innasimuthu ◽  
Ronaldo Anuf Alexander ◽  
Rakesh Varghese ◽  
Murugan Munusamy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Benzene Extract

Parmotrema austrosinense(Zahlbr.) Hale is one of the lichens used as spices and also it has medicinal property. The goal of this investigation was to assess the antioxidant, reducing power, free radical scavenging and antiphyto pathogenic effect of P. austrosinense. Lichen sample was extracted with different solvents - benzene, methanol, chloroform and acetone. The benzene extract showed a strong antioxidant activity (5.665±0.0065) followed by chloroform extract (3.648±0.0029), acetone extract (2.770±0.0078) and methanol extract (2.430± 0.0046). The methanolic extract of lichen showed the highest reducing power followed by the acetone and nonpolar solvents like chloroform and benzene. Among the four solvents chosen, methanol extract of lichen showed the highest free radical scavenging activity followed by acetone, chloroform and benzene. The antimicrobial activity non-polar solvents like chloroform and benzene extract showed highest antimicrobial activity against three tested fungi (Fusarium oxysporum, Rhizoctonia solani, Sclerotium rolfsii) and bacterial plant pathogens(Bacillus sp.,Xanthomonas phaseoli and Erwinia chrysanthemi)followed by methanol and acetone extracts and streptomycin was used as a control antibiotic.

scholarly journals PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

2019 ◽  
pp. 55-57
Author(s):  
ACE BAEHAKI ◽  
SHANTI DWITA LESTARI ◽  
NORYATI SIREGAR
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Power Method ◽  
Activity Assessment ◽  
Sample Extraction ◽  
Phytochemical Compounds

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method. Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171. Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

scholarly journals Phytochemical Profile And Antioxidant Activity Of Guazuma ulmifolia Leaves Extracts Using Different Solvent Extraction

2020 ◽  
pp. 171
Author(s):  
Mohamad Rafi ◽  
Nadya Meitary ◽  
Dewi Anggraini Septaningsih ◽  
Maria Bintang
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolics ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
The Body ◽  
Ftir Spectrum ◽  
Dry Powder ◽  
Guazuma Ulmifolia ◽  
Phytochemical Profile

Guazuma ulmifolia is one of the common tropical plants that has long been used as a traditional medicine to reduce body weight as slimming herbs and to lower the cholesterol in the body. The leaves of G. ulmifolia contain phenolics compound such as flavonoids and tannin that contributes to its biological activities. In this research, we determined the content of total phenolics, flavonoids, tannins, antioxidant activity, and FTIR spectrum profile from four extracts of G. ulmifolia leaves. The extraction method used in this study was stepwise maceration with different solvents n-hexane, ethyl acetate, ethanol, and water. The content of total phenolics and flavonoids were found high in ethanol extract of G. ulmifolia leaves with 35.42 and 44.85 mg QE/g dry powder, respectively. The highest content of total tannins was obtained in ethyl acetate extract of G. ulmifolia leaves as 0.55%. Antioxidant activity from four extracts of G. ulmifolia leaves was measured using DPPH, CUPRAC, and reducing power method. The highest antioxidant activity for DPPH and CUPRAC method was obtained in ethyl acetate extract with the antioxidant capacity as 55.47 and 98.17 µmol trolox/g dry powder, respectively. While using reducing power assay gives the capacity 176.75 µmol trolox/g dry powder in the water extract. The pattern of FTIR spectra from four extracts of G. ulmifolia leaves gives the distinct characteristics of each spectrum profile. Principal component analysis using FTIR spectrum shows good clustering for each extract with 94% data variability (PC1= 77% and PC2 = 17%). It can be concluded that each extract of G. ulmifolia leaves gives a distinct phytochemical profile (phenolics content, flavonoids, tannins, and FTIR spectrum) that contributes to the antioxidant activity. This antioxidant activity mainly influenced by the concentration of phenolic compounds, which is known to have antioxidant properties.

scholarly journals Phytochemical Composition, Antioxidant, and Antimicrobial Attributes of Different Solvent Extracts from Myrica esculenta Buch.-Ham. ex. D. Don Leaves

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 357 ◽  
Author(s):  
Kabra ◽  
Sharma ◽  
Hano ◽  
Kabra ◽  
Martins ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Antioxidant Potential ◽  
Ftir Analysis ◽  
Fungal Strains

Background: Plant diversity is a basic source of food and medicine for local Himalayan communities. The current study was designed to assess the effect of different solvents (methanol, ethyl acetate, and water) on the phenolic profile, and the corresponding biological activity was studied. Methods: Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2″-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) assay, while the antimicrobial activity was evaluated by disk diffusion method using various bacterial and fungal strains. Results: The outcomes demonstrated that methanol acted as the most effective solvent for polyphenols extraction, as strengthened by the liquid chromatography and mass spectroscopy (LC-MS) and fourier transform infrared spectroscopy (FTIR) analysis. M. esculenta methanol extract showed the highest DPPH and ABTS radical scavenger antioxidant activity with IC50 values of 39.29 μg/mL and 52.83 μg/mL, respectively, while the ethyl acetate and aqueous extracts revealed minimum antioxidant potential. Methanol extract also revealed higher phenolic content, 88.94±0.24 mg of equivalent gallic acid (GAE)/g), measured by the Folin–Ciocalteu method, while the minimum content was recorded for aqueous extract (62.38±0.14 GAE/g). The highest flavonoid content was observed for methanol extract, 67.44±0.14 mg quercetin equivalent (QE)/g) measured by an aluminum chloride colorimetric method, while the lowest content was recorded for aqueous extract (35.77±0.14 QE/g). Antimicrobial activity findings also reveal that the methanol extract led to a higher inhibition zone against bacterial and fungal strains. FTIR analysis reveals the presence of various functional groups, viz. alkenes, amines, carboxylic acids, amides, esters, alcohols, phenols, ketones, carboxylic acids, and aromatic compounds. This FTIR analysis could serve as a basis for the authentication of M. esculenta extracts for future industrial applications. Compounds identified by LC-MS analysis were gallic acid, myricanol, myricanone, epigallocatechin 3-O-gallate, β-sitosterol, quercetin, p-coumaric acid, palmitic acid, n-pentadecanol, n-octadecanol, stigmasterol, oleanolic acid, n-hexadecanol, cis-β-caryophyllene, lupeol, and myresculoside. Conclusion: This study suggests that the methanolic extract from M. esculenta leaves has strong antioxidant potential and could be a significant source of natural antioxidants and antimicrobials for functional foods formulation.

scholarly journals Invitro Antimicrobial and Antioxidant Activities of Salvadora persica (Meswak) Roots, Leaves and Stems Extracts

2021 ◽  
Vol 10 (9) ◽  
pp. 498-509
Author(s):  
Dipeshkumar Patel Falaknaaz Shaikh
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Antimicrobial Property ◽  
High Antimicrobial Activity ◽  
Salvadora Persica ◽  
Cell Tissue ◽  
Phenolics Content ◽  
Harmful Side Effect

As microorganisms have developed the inherent ability to develop and adopt a mechanism of resistance against antibiotic. The harmful side effect of antibiotic including their cost of drug development have slowly shifted toward the plant derived phytochemical based medicines. Screening of antimicrobial property of medicinal plants S. persica gives a positive result against the different species of bacteria (S. aureus, P. aeruginosa) and fungi (A.niger, Fusarium). Firstly, a study of phytochemicals shows that the important part to prevent and protect the plant against the microorganisms. Secondly, the importance of phytochemicals of S.perica provide the information about the compound which are responsible for the antimicrobial activity like alkaloids, phenolics, flavonoids etc. Finally, an antioxidant activity involves in the prevention of plant cell tissue damage. Antioxidant activity is measured by DPPH. The total phenolics content of this plant was good and there for this, has high antimicrobial activity. The S.persica has many applications in mouth associated problems, useful to produce antiplaque, analgesic, anticonvulsant, antimycotic, cytotoxic, antifertility, deobstruent, carminative, diuretic, and also applicable in rheumatism.

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