scholarly journals Enrichment of mesophilic acidophiles from the Underground Copper Mine Bor

2009 ◽  
Vol 63 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Vesna Conic ◽  
Vladimir Cvetkovski ◽  
Emina Pozega ◽  
Milovan Vukovic ◽  
Milena Cvetkovska

In this work, autotrophic growth of mesophilic acidophiles from the Underground Copper Mine Bor was performed. Two selected solution samples collected from the 'Tilva Ros' ore body were prepared in a 9K nutrient medium (Silverman and Lundgren, 1959). The first sample TR k-16 was obtained during the hole drilling of the ore body, and the second TR k-31 from the drainage channel. Two samples of 9K media (Silverman and Lundgren, 1959) were inoculated with two selected solution samples from the underground mine Tilva Ros. Inoculated culture media were incubated without prior autoclaving in the period of 6 days at a temperature of 28 ?C with purging air through the system with enough oxygen and carbon dioxide. Oxidation rate of ferrous ions in the first 3 days of incubation was 14.8 and 10.7 wt.% Fe2+/day, the next 3 days 17.3 and 13.6 and for the total period of 6 days 98.3 and 74.8 wt.% for the first and second sample, respectively, i.e. 100 wt.% with initial percentage of ferrous ion in each medium. After centrifugation of enriched samples of culture media at 3000 rpm for 5 min, a plenty of mesophilic acidophiles were determined by microscopic method. According to Karavaiko [6], in the processes of incubition for 9K nutrient solution cells number reach a value of 108 cells/cm3.

2016 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Nur Amalina binti Mustafa ◽  
Muhammad Ashraf bin Redzuan ◽  
Muhamad Hazim bin Zuraimi ◽  
Muhamad Shuhaimi bin Shuib ◽  
Shahnaz Majeed ◽  
...  

Objective: Owing to the habit of consuming ready food among the citizens of Malaysia a study was conducted to evaluate 20 samples of canned soya milk for the presence of possible microbial content. The samples were collected randomly from shopping malls, restaurants and kiosk in Ipoh Malaysia. Methods: All samples collected across Ipoh, were subjected to test for presence bacteria in nutrient agar, blood agar and macConkey media. The possible microbial load was swapped from surface and soya milk content with a sterile cotton and streaked on nutrient agar, blood agar and macConkey culture media. The streaked petri plates were incubated for 48 hours at 37oC. Results: The study revealed negative microbial growth in all except two samples from the surface and soya milk content collected from a restaurant in nutrient agar and blood agar medium. The presence of microbes was conformed as gram positive staphylococcus sp. through gram staining. The positive growth may be imputed to poor storage condition at the restaurant. Conclusion: It can be computed from the study that the majority of the samples were free from bacterial growth, suggesting strong in house quality control mechanism at the processing unit and exquisite storage conditions in malls and kiosk suggesting that soya milk available in malls and kiosk are fit for human consumption.


Minerals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 443 ◽  
Author(s):  
Jinmiao Wang ◽  
Hui Zhao ◽  
Lin Bi ◽  
Liguan Wang

Modeling ore body in 3D is the basis of digital intelligent mining. However, most existing three-dimensional mining software uses the contour approach that requires too much man–machine interaction and difficult partial updating. Moreover, accounting for uncertainty and low geometric quality picking is very difficult in the direct contour approach. Therefore, an implicit modeling approach to automatically build the three-dimensional model for ore body by means of spatial interpolation directly based on the geological borehole data with Hermite radial basis function (HRBF) algorithm as the core is proposed. Furthermore, in order to solve the problems of weak continuity of models due to the long-distance original boreholes as well as the boundary-point normal solution error, the densification of original borehole data with the virtual borehole as well as the calculation of point-cloud normal direction based on the adjacent hole-drilling method is proposed. The verification of two mine engineering projects and comparison with the explicit modeling results show that this approach could realize the automatic building of three-dimensional models for the ore body with high geometric quality, timely update and accurate results.


2013 ◽  
Vol 6 (1) ◽  
pp. 30 ◽  
Author(s):  
Madhur Nayan ◽  
Mohamed A. Elkoushy ◽  
Sero Andonian

Introduction: The current Canadian Urological Association (CUA)guideline recommends two 24-hour urine collections in the metabolic evaluation for patients with urolithiasis. The aim of the present study was to compare two consecutive 24-hour urine collections in patients with a history of urolithiasis presenting to a tertiary stone clinic.Methods: We retrospectively reviewed 188 patients who had two24-hour collections upon presentation between January 2010 and December 2010. Samples were collected on consecutive days and examined for the following 11 urinary parameters: volume, creatinine, sodium, calcium, uric acid, citrate, oxalate, potassium, phosphorous, magnesium and urea nitrogen. For each parameter, the absolute value of the difference between the two samples rather than the direct difference was compared with zero. Similarly, the percent difference between samples was calculated for each parameter.Results: The means of the absolute differences between the twosamples were significantly different for all 11 urinary parameters(p < 0.0001). The percent differences for all urinary parametersranged from 20.5% to 34.2%. Furthermore, 17.1% to 47.6% ofpatients had a change from a value within normal limits to anabnormal value, or vice-versa. Significance was maintained when patients with incomplete or over-collections were excluded.Conclusions: Significant variations among the two 24-hour urinecollections were observed in all of the 11 urinary parameters analyzed. This variation may change clinical decision-making in up to 47.6% of patients if only a single 24-hour urine collection is obtained. The present study supports the CUA guideline of performing two 24-hour urine collections.


1968 ◽  
Vol 70 (3) ◽  
pp. 391-392 ◽  
Author(s):  
K. J. Carpenter ◽  
C. L. Johnson

Although beans are grown primarily because samples they are richer in protein than the cereal grains, the energy they contribute to mixed diets is also important. An unpublished computer calculation of least-cost formulae for laying hen diets has indicated that if two samples of bean meal were of the same composition, except that one had 300 kcal/lb more metabolizable energy (M. E.) than the other, its worth would be more than £3/ton greater. The actual range of values taken for bean meals in this country is considerably wider than 300 kcal/lb. Bolton (1963) lists a value of 830 kcal/lb, whilst Bolton (1967) lists 1400 kcal/lb. Titus (1955) lists 610 and 640 kcal/lb though specifying that these values refer to navy beans and pinto beans respectively (both being Phaseolus vulgaris). Bletner, Chalhoub & Geff (1963) were unable to find any published value for ‘horse’ or ‘field’ beans as (i. e. Vicia faba), the type normally used for animal feeding in Britain.


Author(s):  
Rusydi Indra ◽  
Erniwati Ibrahim ◽  
ApikIndarty Moedjiono ◽  
Syamsuar M ◽  
Agus Bintara Birawida ◽  
...  

Purpose: This study aims to determine the density of Aedes aegypti larvae based on the knowledge, attitudes and actions of terminal managers at the Regional Terminal Daya Makassar City. Methods: The research design used was descriptive observational. There are two samples in this study, namely the container sample and the terminal manager sample. The sampling technique for containers used total sampling method and for terminal managers, purposive sampling method was used. Data were analyzed by univariate. Results: The results of the analysis showed that the number of positive containers for larvae was 69 (CI = 33%) with a value of DF = 8 and was a high density category. A total of 9 respondents (39.1%) had good knowledge and 14 respondents (60.9%) had poor knowledge. A total of 10 respondents (43.5%) had a good attitude and as many as 13 respondents (56.5%) had a bad attitude. In terms of action, (4.3%) of the respondents reflected good, while 22 respondents (95.7%) had actions in the unfavorable category. Conclusion: It is concluded that increasing public knowledge is essential to eradicate mosquito nests in the community. By enhancing the knowledge of the individuals, the  PSN activities carried out routinely can break the life cycle of the mosquitoes that can cause DHF.The level of education that the terminal manager has, the better the ability to absorb the information provided will affect the knowledge held by the terminal manager.


Metals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1112 ◽  
Author(s):  
Kevin Pérez ◽  
Norman Toro ◽  
Eduardo Campos ◽  
Javier González ◽  
Ricardo I. Jeldres ◽  
...  

Exotic type deposits include several species of minerals, such as atacamite, chrysocolla, copper pitch, and copper wad. Among these, copper pitch and copper wad have considerable concentrations of manganese. However, their non-crystalline and amorphous structure makes it challenging to recover the elements of interest (like Cu or Mn) by conventional hydrometallurgical methods. For this reason, black copper ores are generally not incorporated into the extraction circuits or left unprocessed, whether in stock, leach pads, or waste. Therefore, to dilute MnO2, the use of reducing agents is essential. In the present research, agitated leaching was performed to dissolve Mn of black copper in an acidic medium, comparing the use of ferrous ions and tailings as reducing agents. Two samples of black copper were studied, of high and low grade of Mn, respectively, the latter with a high content of clays. The effect on the reducing agent/black copper ratio and the concentration of sulfuric acid in the system were evaluated. Better results in removing Mn were achieved using the highest-grade black copper sample when working with ferrous ions at a ratio of Fe2+/black copper of 2/1 and 1 mol/L of sulfuric acid. Besides, the low-grade sample induced a significant consumption of H2SO4 due to the high presence of gangue and clays.


2020 ◽  
Vol 12 (3) ◽  
pp. 741-751
Author(s):  
Samira MEZIANI ◽  
Souad SAIDANI ◽  
Noreddine MENADI ◽  
Hayet MEHIDDA ◽  
Mohammed ZAIRI ◽  
...  

From this context we have been interested in biochemical and phytochemical parameters of local and foreign varieties of wheat bran whose purpose is to search to see if there are intraspecific and interspecific varietal differences. The study concerned five varieties of soft wheat bran selected at CCLS Sidi Bel Abbés and consumed in many regions of Algeria. The biochemical compounds of grains (proteins, cellulose, ash content and phytochemical (total phenols, flavonoids and antioxidant activity) were determined in all varieties. The wheat bran, the subject and material of this study, was obtained from whole wheat grains collected from three varieties of soft wheat and used in our case to produce and extract the maximum of soft wheat bran. The varieties used were ‘HD’ (SWBHD) and ‘Anzar’ (SWBA) local variety and one imported, ‘Habbour’ variety (SWBHB). Two samples of marketed soft wheat bran, imported, were provided in this work to compare their nutritional components. Different techniques and methods were used in this experimental study (infra-red approach spectrophotometer, flame spectrophotometer). The results obtained show that ‘Eriad’ soft wheat bran (SWBE) is rich in protein with a variation of 15.78% at 18.07%; and is slightly elevated compared to other samples. Regarding the results obtained for cellulose, a high value was recorded for (SWBTAZ) variety 11.3%, and a lower one for (SWBHB) marketed at a value of 3.6%. The maximum concentration of potassium and sodium was obtained in the SWBAZ variety with a level of 3.16 mg/l and 30.36 mg/l respectively. The evaluation of phytochemicals has shown the presence of considerable amounts of polyphenols and flavonoids qualitatively and quantitatively. SWBAZ variety was very rich in total polyphenols, flavonoids and DPPH respectively with values of (1.101±0.01 mg EAG/g) and (0.174±0.001 EC/g) (1.39±0.01 EAG/g) compared to the different varieties studied. In conclusion, the SWBAZ variety could thus be considered, in our case, as an important source of phytonutrients.


1999 ◽  
Vol 183 ◽  
pp. 248-248
Author(s):  
Paul Haines ◽  
G.F.R. Ellis

We compare the density fields from POTENT and IRAS. We vary the smoothing scale, and use a non-parametric test to obtain a value for the linear bias parameter b, as a function of offset c, which arises because the normalisation volumes for the two samples are different (Dekel et al., 1993). The smoothed fractional overdensities are related by δP = δI/b + c.


Sign in / Sign up

Export Citation Format

Share Document