Reference limits for routine haematological measurements in pre-school and school-children living at an urban area
The aim of this study is to estimate 95% reference limits for eight haematological constituents of blood in order to provide medically reliable evaluation of haematological laboratory results. Blood counts were performed using an automated haematology analyzer (Cell-Dyn 1500) on capillary blood samples from 1918 children (1?16 years old) residing in a region of Novi Beograd. Physiological variations corresponding to age and sex were studied as the most important factors affecting biological variation in haematological constituents of blood. Children were divided in four groups according to age: 1?3 yrs (281 boys and 241 girls), 4? 6 yrs (196 boys and 150 girls), 7?12 yrs (322 boys and 368 girls) and 13 ?16 yrs (174 boys and 186 girls). In this study all investigated parameters were not sex dependent until the age of 13. Boys between ages 13 and 16 years have higher mean values of haemoglobin (143.81 ? 13.40 g/L) and red blood count (4.81 ? 0.49 ? 1012/L), but lower MCV (86.6 ? 5.27 fL) than girls in same age group (136.81 ? 13.42 g/L, 4.57 ? 0.47 ? 1012/L and 88.0 ? 6.96 fL respectively). There was no significant difference according to sex in the oldest group for mean values of haematocrit (boys 0.42 ? 0.049 L/L and girls 4.57 ? 0.47 L/L), MCH (boys 29.9 ? 1.92 pg and girls 30.2 ? 1.96 pg), MCHC (boys 345.9 ? 18.4 g/L and girls 342.2 ? 19.7 g/L), platelet (boys 219.8 ? 49.6 ? 109/L and girls 225.3 ? 59.2 ? 109/L) and white blood count (boys 8.0 ? 2.60 ? 109/L and girls 8.01 ? 2.08 ? 109/L). Haemoglobin level, red blood count, haematocrit, MCV, MCH and MCHC increased with age. Levels of platelet count and white blood count decreased with age.