Antimicrobial and antioxidant activity of brown algae from the Aegean sea

The present study was conducted to evaluate the antioxidant and antimicrobial activity of methanol, dichloromethane and hexane extracts, as well as the essential oils of brown algae (Phaeophyta) Colpomenia sinuosa, Dictyota dichotoma, Dictyota dichotoma var. implexa, Petalonia fascia and Scytosiphon lomentaria. The essential oil of the macroalgae was obtained by steam distillation and analyzed by GC and GC/MS. The antioxidant activity of the algal extracts was determined using the procedures of inhibition of ?-carotene bleaching and ABTS+ methods. The antioxidant effects of the extracts were compared with those of commercial antioxidants, such as butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA) and ?-tocopherol. The hexane extracts of D. dichofoma var. implexa had a higher phenolic content than the other extracts. The dichloromethane extract of S. lomentaria was found to be more active in the decolorization of ABTS+ than the other extracts and generally the dichloromethane extracts were more active than the methanol and hexane extracts. Antimicrobial activities of the extracts were assessed against Gram (+) and Gram (-) bacteria and one yeast strain by the disk diffusion method. According to the results, the dichloromethane extracts generally showed more potent antimicrobial activity than the methanol and hexane extracts at concentrations 1.5 and 1.0 mg/disk.

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ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37495 ◽

2020 ◽

pp. 78-84

Author(s):

FAVIAN BAYAS-MOREJON ◽

ANGELICA TIGRE ◽

RIVELINO RAMON ◽

DANILO YANEZ

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Potassium Phosphate ◽

Natural Antioxidants ◽

Butylated Hydroxytoluene ◽

Penicillin G ◽

Diffusion Method ◽

E Coli ◽

Antibacterial And Antioxidant Activity

Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts. Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer. Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%. Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.

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Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48111782 ◽

2020 ◽

Vol 48 (1) ◽

pp. 53-65

Author(s):

Tatjana D. GOLUBOVIĆ ◽

Gordana S. STOJANOVIĆ ◽

Dušanka V. KITIĆ ◽

Bojan K. ZLATKOVIĆ ◽

Dragana R. PAVLOVIĆ ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Salmonella Enteritidis ◽

Ethanol Extract ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Fungal Organisms ◽

Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

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Antimicrobial activity of raw soybean, soybean flour and roasted soybean extracted by ethanol-hexane method

British Food Journal ◽

10.1108/bfj-10-2016-0499 ◽

2017 ◽

Vol 119 (10) ◽

pp. 2277-2286 ◽

Cited By ~ 1

Author(s):

Sireerat Laodheerasiri ◽

Nirasha Horana Pathirage

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Antimicrobial Compounds ◽

Soybean Flour ◽

Hexane Extraction ◽

Disk Diffusion Method ◽

Content Type ◽

E Coli ◽

Inhibitory Compounds

Purpose The purpose of this paper is to determine the antimicrobial compounds extracted from various types of soybean materials; raw soybean, soybean flour and roasted soybean. Bacteria growth inhibition was tested in E.coli and S. aureus by disk diffusion method. Efficiency of antimicrobial activities were compared among the extracted solution. Design/methodology/approach Soybean (Glycine max) isoflavones contain the ability to inhibit the growth of many strains of microorganisms. The solid-liquid extraction, ethanol-hexane extraction, was modified to isolate the inhibitory compounds from the three different types of soybean materials. All crude extracts at various concentrations performed under different extracted solutions (75, 50, 25, 12.5, 6.25 and 3.125 percent) were tested with E. coli. and S. aureus to determine the antimicrobial activities and the minimum inhibition concentration (MIC). Disk diffusion method was chosen to study the antimicrobial activity of isoflavones. Findings Soybean flour extract inhibited the growth of E. coli and S. aureus more effective than roasted soybean and raw soybean, respectively. The MIC of inhibitory compounds extracted from soybean flour was 6.25 percent (0.031 mg/ml), roasted soybean was 25 percent (0.125 mg/ml) and raw soybean was 50 percent (0.250 mg/ml). Crude extracts of soybean flour and roasted soybean showed better results than raw soybean. All the three of extracted soybeans inhibited the growth of E. coli better than S. aureus. Originality/value Ethanol-hexane extraction was successfully used to isolate the antimicrobial compounds from raw soybean, soybean flour and roasted soybean. Comparison of antimicrobial activity showed that soybean flour contained the highest inhibition activity.

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In vitro antimicrobial activity of douro wines against clinical Helicobacter Pylori Strains

Nova ◽

10.22490/24629448.5499 ◽

2021 ◽

Vol 19 (37) ◽

pp. 121-134

Author(s):

Lidia Po Catalao Dionisio ◽

Alejandro Manuel Labella ◽

María Palma ◽

Juan José Borrego

Keyword(s):

Antimicrobial Activity ◽

Helicobacter Pylori ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Horse Blood ◽

H Pylori

Aim. In vitro antimicrobial activities of seven wines (5 reds and 2 whites) from the Douro region (Iberian Peninsule) against eleven clinical strains of Helicobacter pylori were evaluated. Methods. The disk diffusion method, using Columbia Agar supplemented with horse blood (CAB), were used to determine the antimicrobial properties of some wine components against H. pylori strains. Potential interactions of antioxidants contained in the wines and two antimicrobials (amoxicillin and metronidazole) were studied by the disk diffusion method. Results. All the tested strains showed growth in CAB supplemented with 9% of the tested wines but none of them grew in media supplemented with 45% and 67.5% of wine. Similarly, all the tested strains grew in media with the concentration of proanthocyanidins present in the different types of the studied wines. The Minimal Inhibitory Concentration (MIC) values of the wine antioxidant components tested (benzoic acid, catechin, quercetin, and resveratrol) indicate that resveratrol was the most powerful inhibitory substance against H. pylori. An effect of potentiation between amoxicillin and metronidazole and the antioxidants tested was also established. The interaction of amoxicillin and resveratrol or metronidazole and catechin increased the antimicrobial activity against H. pylori. Conclusions. The results obtained suggested a potential role of resveratrol as a chemopreventive agent for H. pylori infection.

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Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

Current Bioactive Compounds ◽

10.2174/1573407214666180802125128 ◽

2020 ◽

Vol 16 (2) ◽

pp. 109-114

Author(s):

Souad Khebri ◽

Naima Benkiki ◽

Mouloud Yahia ◽

Hamada Haba

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Positive Control ◽

Diffusion Method ◽

Β Carotene

Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

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Comparison of Antimicrobial Activity ofAllium sativumCloves from China and Taşköprü, Turkey

Advances in Pharmacological Sciences ◽

10.1155/2018/9302840 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Ali Yetgin ◽

Kerem Canlı ◽

Ergin Murat Altuner

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

In this study, antimicrobial activities of two different samples ofAllium sativumL. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species ofBacillus,Enterobacter,Enterococcus,Escherichia,Klebsiella,Listeria,Pseudomonas,Salmonella, andStaphylococcuswere used for testing antibacterial activity. In addition, antifungal activity againstCandida albicanswas also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.

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Uji Aktivitas Antimikroba dan Antioksidan dari Ekstrak Etanol Daun Keji Beling (Strobilanthes crispus BI)

Talenta Conference Series Science and Technology (ST) ◽

10.32734/st.v2i2.511 ◽

2019 ◽

Vol 2 (2) ◽

Author(s):

Rumondang Bulan ◽

Firman Sebayang ◽

Emma Zaidar Nasution ◽

Putri Wulandari

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Candida Albicans ◽

Bacillus Cereus ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Microsporum Gypseum ◽

Disk Diffusion Method ◽

Strobilanthes Crispus

Uji aktivitas antimikroba dari ekstrak etanol daun keji beling (Strobilanthes crispus BI) telah dilakukan terhadap mikroba Candida albicans, Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae dengan metode difusi cakram. Uji aktivitas antioksidan ekstrak etanol daun keji beling dilakukan dengan metode DPPH. Daun keji beling yang telah halus, diekstraksi dengan metode maserasi menggunakan pelarut etanol 96% selama 2 hari, dan dipekatkan dengan rotary vacum evaporator. Uji aktivitas antimikroba dilakukan dengan variasi konsentrasi 100; 200; 300; 400; 500 mg/ml. Hasil skrining fitokimia ekstrak etanol daun keji beling menunjukkan hasil positif terhadap senyawa golongan alkaloid dan fenolik. Hasil penelitian menunjukkan ekstrak etanol daun keji beling efektif terhadap mikroba Candida albicans dan tidak efektif terhadap mikroba Microsporum gypseum, Bacillus cereus, dan Shigella dysentriae. Ekstrak etanol daun kejibeling mampu menghambat pertumbuhan Candida albicans dengan diameter zona bening untuk konsentrasi 100; 200; 300; 400; 500 mg/ml masing-masing adalah 11,53; 13,96; 15,46; 16,80; dan 18,20 mm. Uji aktivitas antioksidan ekstrak etanol daun keji beling dengan metode DPPH menunjukkan nilai IC50 sebesar 155,2 ppm. The antimicrobial and antioxidant activity evaluation of leaves ethanol extract of KejiBeling (Strobilanthescrispus BI) has been carried out. The leaves of KejiBeling was grounded into powder and extracted using ethanol 96% (v/v) for 2x24 h and concentrated with rotary vacum evaporator. The antimicrobial activity was performed against several microbes, i.e. Candida albicans, Microsporumgypseum, Bacillus cereus, andShigella dysentriaeusing disk diffusion method. The antimicrobial activity was performed using several concentrations of leaves extract from 100-500mg/mL.The phytochemical screening showed the presence of alkaloid and phenolic group intheleaves ethanol extract of KejiBeling. The leaves ethanol extract of KejiBeling was antimicrobial active against Candida albicans with inhibition zone diameter of 11,53; 13,96; 15,46; 16,80; and 18,20 mm for the concentration of 100; 200; 300; 400; 500 mg/ml, respectively. The antioxidant activity of leaves ethanol extract showed IC50 value of 155,2 ppm

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In Vitro Comparative Study on the Antibacterial and the Antioxidant Activity of Pergularia tomentosa L.

Asian Journal of Research in Chemistry ◽

10.52711/0974-4150.2021.00049 ◽

2021 ◽

pp. 285-291

Author(s):

Souad Belakehal ◽

Brahim Labed ◽

Louiza Zenkhri ◽

Khedidja Benzahi ◽

Ahmed Tabchouche

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Constituents ◽

Maximum Activity ◽

Diffusion Method ◽

Disk Diffusion Method ◽

A Value ◽

Antioxidant And Antimicrobial Activity

Ethanolic extracts of Pergularia tomentosa L. were used for the relative analysis of antioxidant and antimicrobial activity against two types of Gram-negative bacteria. (Escherichia coli G (-) and Pseudomonas aeruginosa G (-)) and a gram positive (Staphylococus aureus G (+)). Maximum activity was recorded against Escherichia coli, with a maximum inhibition diameter of 23mm using the disk diffusion method. In addition, the results revealed a higher bacterial activity against Escherichia coli with an inhibition diameter of 23mm compared to standard antibiotics Ampicillin/Nystatin with an inhibition diameter of 11.75mm. The study of the antioxidant activity by the DPPH and FRAP methods showed that vitamin C is the most active with an IC50 equal to 0.010 g/ml, followed by the crude extract, with a value of 0.012 g/ml and finally Tocopherol (VE) which seems to be the least effective with an IC50 of 0.015 g/ml. The results obtained in the present study suggest that Pergularia tomentosa L. can be used in the treatment of diseases caused by the organisms tested. Further chemical and pharmacological investigations can be carried out to isolate and identify the chemical constituents in selected plants responsible for antimicrobial activity.

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Phytochemical screening, antioxidant and antimicrobial activities of grape (Vitis vinifera L.) seed extracts from red globe and Valenci Algerian varieties

Acta periodica technologica ◽

10.2298/apt2051137l ◽

2020 ◽

pp. 137-147

Author(s):

Khadidja Labri ◽

Houria Moghrani ◽

Affaf Kord ◽

Ahmed Doukara ◽

Abdelkrim Gueffai ◽

...

Keyword(s):

Antimicrobial Activity ◽

Vitis Vinifera ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Vitis Vinifera L ◽

Phytochemical Analysis ◽

Disk Diffusion Method ◽

Antioxidant And Antimicrobial Activities ◽

Seed Extracts

Until now, there is no documentation concerning the composition, biological and pharmaceutical activities of Algerian grape (Vitis vinifera L.) seeds. The present study aims to evaluate the phytochemical analysis, antioxidant and antimicrobial activities of grape seed extracts (GSEs) from the Algerian Red globe and Valenci varieties. The total polyphenols content (TPC) in the GSEs was evaluated by spectrophotometry, it was 398.01 ? 18.12 mg GAE/g GSEs and 335.11 ? 11.44 mg GAE/g GSEs for the Red globe and Valenci varieties respectively. The antioxidant activity of GSEs was also evaluated by spectrophotometry on stable free radicals of DPPH. The IC50 for the Red globe variety was 4 ? 0.2 ?g/ml, while for the Valenci variety it was 4.6 ? 0.36 ?g/ml. The antimicrobial activity was determined using the disk diffusion method. Both extracts showed antimicrobial activity against the eight studied strains: Escherichia coli, Pseudomonas aeruginosa, Pasteurella sp., Salmonella sp., Staphylococcus aureus, Streptococcus faecalis, Clostridium sp., and Candida albicans. The extracts produced a clear inhibition zone for all the tested strains. Inhibition diameters greater than 19 mm were registered. The obtained results in this study indicate the differences between the Red globe and Valenci varieties, in terms of polyphenol content, antioxidant and antimicrobial activities in Algerian GSEs.

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Composition, Antioxidant and Antimicrobial Activities of Methanol Extracts of Some Acinos Miller Species

Natural Product Communications ◽

10.1177/1934578x1400900539 ◽

2014 ◽

Vol 9 (5) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Tatjana Golubović ◽

Radosav Palić ◽

Dušanka Kitić ◽

Gordana Stojanović ◽

Bojan Zlatković ◽

...

Keyword(s):

Antioxidant Activity ◽

Salmonella Enteritidis ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Serbia And Montenegro ◽

Methanol Extracts ◽

Methanolic Extracts ◽

Aerial Parts ◽

The Common

GC and GC/MS analyses of the methanol extracts obtained from the aerial parts of six Acinos Miller species from Serbia and Montenegro were performed. Seventy-four constituents, accounting for 84.9-99.0% of the total composition of the extracts, were identified. The common feature of the A. suaveolens, A. majoranifolius and A. alpinus methanol extracts was the high content of monoterpenes, while the common feature of the A. graveolens, A. arvensis and A. hungaricus methanol extracts was the prevalence of sesquiterpenes. The total flavonoids, polyphenols and tannins content, as well as antioxidant activity (FRAP and DPPH assay) of the methanolic extracts were investigated. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all polyphenol classes examined. A disk diffusion method was used for the evaluation of the antimicrobial activities of the extracts against a panel of microorganisms (bacteria: Bacillus subtilis, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus, Sarcina lutea, Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enteritidis and Escherichia coli; fungi: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae). The preliminary bioassay results indicated that the methanol extract of A. alpinus could be a possible source of antioxidant and antimicrobial compounds.

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