Evaluation of lipid status parameters in the working population of Gradiska municipality with age and gender distributions

Introduction The aim of this investigation was to establish the frequency of hyperlipoproteinemia, as well as the distribution of desired, elevated and high risk values of certain lipid status parameters among working population of the Gradiska municipality with age and sex distribution. Material and methods This investigation included 250 workers,109 male and 141 female, 25-60 years of age of the Gradiska municipality on a regular checkup. Standard biochemical methods were used to determine values of total serum cholesterol, triglyceride and HDL cholesterol. LDL cholesterol and LDL to HDL cholesterol ratio were calculated. Results Total serum cholesterol was elevated in 44.04% of males and 44.68% of females, triglyceride in 20.02% of males and 19.15% of females and LDL cholesterol in 31.96% of males and 21.43% of females. High risk values of total cholesterol were established in 43.12% of males and 39.01% of females, and of triglyceride in 37.61% of males and 9.93% of females. HDL cholesterol was decreased in 55.96% of males and 41.84% of females, while highly decreased values were established only in 5.5% of males and 1.42% of females. Increase of total cholesterol and LDL cholesterol correlated with workers' age, but values of triglyceride did not. Hyperlipoproteinemia was evident in 76.4% of examinees. Discussion All tested parameters vary dramatically from desired levels. These results are probably associated with unhealthy food habits and lifestyle. Conclusion Our results point to the need to perform regular laboratory diagnostic procedures and routine check-ups in the working population.

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Evaluation of some biochemical changes in the serum of phensedyl addicts of Gaibandha District, Bangladesh

Journal of Bio-Science ◽

10.3329/jbs.v20i0.17656 ◽

2014 ◽

Vol 20 ◽

pp. 57-65

Author(s):

Md Rashidul Hasan ◽

Parvez Hassan ◽

Md Abdul Jalil Miah

Keyword(s):

Serum Cholesterol ◽

Biochemical Parameters ◽

Ldl Cholesterol ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Triglyceride ◽

Hdl Cholesterol ◽

Total Serum ◽

Total Serum Cholesterol ◽

P Value

Context: Abuse of the drug, Phensedyl like any other drug might exert adverse effects on vital organs of th e h u m an body like liver, kidney and heart. Objectives: To determine the effects of Phensedyl intake on the serum biochemical parameters of the addicts in order to access for damages of vital human organs like liver, kidney and heart. Materials and Methods: Study population consisted of 127 male Phensedyl addicts within the ages of 1855 years of defined criteria from Gaibandha district, a Northern part of Bangladesh, during July 2009 to December 2011. Fifty (50) non-drug dependent healthy men of matched age, height, and socioeconomic status were included as controls from the same community. Biochemical parameters analyzed were Serum creatinine, SGOT, SGPT and Lipid profiles (total serum cholesterol (TC), Serum triglyceride (TG), serum high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol determined by semiautomatic biochemistry analyzer using commercially available kits Results: Abuse of Phensedyl appears not to hamper the normal renal and liver function in most of the addicts even after 8 years of Phensedyl intake irrespective of age except in case of 4 abusers. Serum total cholesterol (TC) remained almost unchanged among the addicts abusing Phensedyl for less than 8 years. But very strikingly, addicts taking Phensedyl for more than 8 years had higher trends in serum cholesterol i.e. more than 200 ml/dl. Of the addicts, 44.36% abusing Phensedyl for less than 8 years had normal triglyceride (TG) values whereas, in 45.66% addicts abusing the drug for more than 8 years had clinically very significantly elevated triglyceride levels, which was also found to be statistically very significant (p value =0.0001), indicating the risk of developing cardiac diseases. Of the total addicts 53.53% had low levels of HDL cholesterol, which is clinically and statistically found to be very significant (p value =0.002). Of the addicts 43% taking the drug for less than 8 years had normal LDL levels but significantly elevated values were recorded in 34% of the abusers who had been taking the drug for more than 8 years. Conclusion: Long time (> 8 years) Phensedyl abusers are at the high risk of developing Brain stroke, Coronary Heart Disease (CHD), Ischemic stroke or transient ischemic attack (TIA) as there is the triad of: Elevated LDL cholesterol, Low HDL cholesterol and elevated Triglyceride. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17656 J. bio-sci. 20: 57-65, 2012

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NIGELLA SATIVA (KALONJI)

The Professional Medical Journal ◽

10.29309/tpmj/2011.18.01.1882 ◽

2011 ◽

Vol 18 (01) ◽

pp. 142-146

Author(s):

MUHAMMAD ANWAR BURIRO ◽

MUHAMMAD TAYYAB ◽

ALLAH DITTA

Keyword(s):

Serum Cholesterol ◽

Ldl Cholesterol ◽

Nigella Sativa ◽

Density Lipoprotein ◽

Hdl Cholesterol ◽

Total Serum ◽

Control Group ◽

Total Serum Cholesterol ◽

Blood Samples ◽

Cholesterol Levels

The increased level of LDL-c in the serum has a high risk and the increased serum HDL-c level has a low risk for the development of atherosclerosis. The effect of Nigella Sativa on levels of cholesterol fractions were determined in this study on rats. Methods: 24 albino rats of 08 weeks age having equal number of males and females were kept at optimum atmospheric condition. The blood samples were taken at the start and different control and experimental diets were given for 24 weeks. The experimental diets were added with Nigella Sativa as 30 mg/kg body weight. The blood samples were taken at the end of study. The blood samples drawn at the start and end of the study were estimated for serum cholesterol. The results of control and experimental groups were compared. Results: Total serum cholesterol in the control group showed increase from 8.3±3.30 to 13.96±9.3 at 24 weeks. The serum HDL cholesterol showed increase from 44.4±6.12 to 80.45±5.95 level at 24 weeks. The serum LDL cholesterol showed increase from 8.3±3.30 to 13.96±9.3 at 24 weeks. The total serum cholesterol in experimental group was increased from 76.9±6.5 to 117.5± 6.65 at 24 weeks. The serum HDL cholesterol levels was increased from 41.7±4.9 to 83.42±5.92 at 24 weeks as compared with control group. The LDL cholesterol levels were decreased from 12.7±6.9 to 8.5±7.8 at 24 weeks. Conclusions: This study shows significant decrease in serum low density lipoprotein cholesterol level, and increase in serum high density lipoprotein cholesterol levels.

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Effects of Intralipid infusion on rat serum lipoproteins

Laboratory Animals ◽

10.1258/002367794780745290 ◽

1994 ◽

Vol 28 (2) ◽

pp. 138-142 ◽

Cited By ~ 8

Author(s):

Kishor M. Wasan ◽

V. Bruce Grossie ◽

Gabriel Lopez-Berestein

Keyword(s):

Food Intake ◽

Total Cholesterol ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

Venous Catheter ◽

Total Serum ◽

Control Group ◽

Total Serum Cholesterol ◽

Rat Serum ◽

Fischer 344

The purpose of this investigation was to study the effects of continuous Intralipid® infusions on serum HDL and LDL levels in the rat. Male Fischer 344 rats were infused continuously via central venous catheter with 10% Intralipid® for 96 h and 5, or 2.5% Intralipid for 14 days. Blood samples were collected during the infusion period for total serum cholesterol, HDL-, and LDL-cholesterol measurements. Food intake was monitored during the studies. Total cholesterol, HDL-cholesterol and LDL-cholesterol levels were significantly elevated following 96 h of infusion with 10% Intralipid with food intake significantly decreased compared to a control group. In a second experiment, animals received a continuous infusion of either 5% Intralipid, 2.5% Intralipid or 0.45% saline for 14 days. Total cholesterol, HDL-cholesterol and LDL-cholesterol were significantly elevated following 14 days of infusion with 5% Intralipid group compared to controls but food intake remained constant for 12 days with no evident toxicity.

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Short-term Effect of Garlic Extract on Patients with Dyslipidemia

Nepal Medical College Journal ◽

10.3126/nmcj.v21i4.27626 ◽

2019 ◽

Vol 21 (4) ◽

pp. 301-305

Author(s):

A Limbu ◽

GP Rauniar ◽

SK Sharma ◽

DR Panday ◽

BK Shah ◽

...

Keyword(s):

Serum Cholesterol ◽

Ldl Cholesterol ◽

Serum Triglyceride ◽

Hdl Cholesterol ◽

Lipid Lowering ◽

Total Serum ◽

Total Serum Cholesterol ◽

P Value ◽

Vldl Cholesterol ◽

Significant Difference

Dyslipidemia is a metabolic abnormality leading to a persistent increase in the plasma concentration of cholesterol and triglycerides. Dyslipidemia is known to promote atherosclerosis. It is a complex disease and is a major risk factor for adverse cardiovascular events. Allium sativum commonly known as garlic has been found to have several medicinal value including, lipid-lowering property, blood pressure decreasing, anti-diabetic, anti-cancer and anti-oxidant activity. It was a double-blind, two-parallel-group, prospective interventional clinical trial. Total no of 112 patients were enrolled in the study and divided into two equal groups (56 in each group) viz; Group 1 (Lasuna TM) and Group 2 (placebo). Lipid profile was measured at day 0 and 90. Independent–sample‘t’ test was applied to find out the significant difference between the two groups, P-value being 0.05. The mean difference Total Serum Cholesterol, Serum Triglyceride, Serum LDL Cholesterol and Serum VLDL cholesterol on day 0 was nonsignificant with p being 0.539, 0.811, 0.230, 0.770 and 0.811 and on 90th day was significantly lower in group taking garlic with p being 0.001, 0.014, 0.003 and 0.008 respectively whereas Serum HDL cholesterol on 90th day was significantly higher in group taking garlic with p being 0.001. Garlic, when given as supplement decreases Total Serum Cholesterol, Serum Triglycerides, Serum LDL Cholesterol and Serum VLDL Cholesterol and on the other hand it increases Serum HDL Cholesterol.

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Effect of interventions with ingestion of legumes and/or supervised exercise on the lipid profile of young, healthy sedentary women

Colombia Medica ◽

10.25100/cm.v41i3.708 ◽

2010 ◽

pp. 224-234 ◽

Cited By ~ 1

Author(s):

Luis F. Fajardo ◽

Dora G. Castellanos ◽

Myriam Chinchilla ◽

Luz N. Vargas ◽

Martha Guerra ◽

...

Keyword(s):

Lipid Profile ◽

Life Style ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

Serum Levels ◽

Total Serum ◽

Total Serum Cholesterol ◽

Supervised Exercise ◽

Healthy Life Style ◽

Sedentary Women

Objective: To contribute to the knowledge of some aspects of the Healthy Life Style by studying the effects of including legumes in the diet and exercise at two intensity levels, along with the lipid profile of young sedentary women living at 2640 meters above sea level. Materials and methods: The study included a non-randomized clinical trial with four intervention groups: exercise at 45% VO2 peak plus legumes in diet, exercise at 65% VO2 peak plus legumes in diet, only exercise at 65% VO2 peak, and only inclusion of legumes in diet. In each group, 20 to 23 sedentary women were included. The intervention was carried out for four weeks, three days a week. Exercise prescription was based on measurement of VO2 peak by ergospirometry; and the current intervention was monitored with heart-rate monitors. The outcome variables were total serum cholesterol, LDL cholesterol, HDL cholesterol, and triacylglycerols measured at baseline and after two and four weeks of intervention. Results: The measurements taken of participants in the group of exercise at 65% VO2 peak plus legumes in diet showed a reduction of 19.8 mg/dl in total cholesterol, of 21.8 mg/dl of LDL cholesterol, of 20.7 mg/dl of tracylglycerol, and an increase of 6.2 mg/dl of HDL cholesterol. The serum levels of HDL also increased in the group of only exercise at 65% VO2 peak. No significant changes in serum levels were documented for participants in the group with only dietary modifications. Conclusions: The results suggest that different interventions that meet some of the criteria for healthy eating and life style show different effects with regards to the level of change in the lipid profile components.

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Total serum cholesterol by isotope dilution/mass spectrometry: a candidate definitive method.

Clinical Chemistry ◽

10.1093/clinchem/26.7.854 ◽

1980 ◽

Vol 26 (7) ◽

pp. 854-860 ◽

Cited By ~ 97

Author(s):

A Cohen ◽

H S Hertz ◽

J Mandel ◽

R C Paule ◽

R Schaffer ◽

...

Keyword(s):

Mass Spectrometry ◽

Total Cholesterol ◽

Serum Cholesterol ◽

Isotope Dilution ◽

Isotope Dilution Mass Spectrometry ◽

Weight Ratio ◽

Molecular Ions ◽

Total Serum ◽

Total Serum Cholesterol ◽

Definitive Method

Abstract We describe a highly accurate and precise method for determination of total cholesterol in serum by isotope dilution/mass spectrometry. The method was developed for a Study Group of the Committee on Standards of the American Association for Clinical Chemistry, for use in establishing the accuracy of a candidate reference method for total cholesterol, and fulfills their criteria for a definitive method. Cholesterol-d7 is added to serum, with the weight ratio of cholesterol-d7 to total serum cholesterol kept near to 1:1. The esters are hydrolyzed and the cholesterol is separated and converted into the trimethylsilyl ether derivative for measurement by combined gas chromatography/mass spectrometry. The intensity ratio of the molecular ions at m/z 465 and 458 is measured for each sample and for two calibration mixtures, according to a prescribed bracketing protocol. A weight ratio for the sample is obtained by linear interpolation of the ion-intensity ratios, and the total cholesterol is then calculated. The method was applied four times over several weeks to each of five serum pools. Statistical analysis involving consideration of both replication error and variability between weeks gave a coefficient of variation for a single measurement of 0.36%. The absence of interferences in the method was demonstrated by measurements at several other masses.

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Combined effect ofLactobacillus acidophilusandβ-cyclodextrin on serum cholesterol in pigs

British Journal Of Nutrition ◽

10.1017/s0007114515003736 ◽

2015 ◽

Vol 115 (1) ◽

pp. 1-5 ◽

Cited By ~ 10

Author(s):

L. Alonso ◽

J. Fontecha ◽

P. Cuesta

Keyword(s):

Treatment Group ◽

Serum Cholesterol ◽

Ldl Cholesterol ◽

Combined Treatment ◽

Combined Effect ◽

Total Serum ◽

Control Group ◽

Total Serum Cholesterol ◽

Treatment Groups ◽

The Individual

AbstractA total of twenty-four Yorkshire gilt pigs of 6–7 weeks of age were used in a 2×2 factorial experiment to determine the individual and combined effects of the inclusion of two dietary factors (cholesterol rich, 3 %β-cyclodextrin (BCD) andLactobacillus acidophiluscultures) on total cholesterol and LDL-cholesterol levels in blood serum. Pigs were assigned randomly to treatment groups (n6). Total serum cholesterol concentrations decreased after 3 weeks in all the experimental treatment groups, including diets with BCD,L.acidophilusor both. Similar trends were observed for serum LDL-cholesterol concentrations among the experimental treatments. No statistically significant differences from the control group were observed in either total serum cholesterol or LDL-cholesterol concentrations (P<0·05) for each of the individual treatment groups: BCD orL.acidophilus. However, significant differences in total serum cholesterol concentrations were observed when comparing the combined treatment group (BCD andL.acidophilus) with the control group, which consisted of a basal diet and sterile milk. The combined treatment group exhibited 17·9 % lower total serum cholesterol concentration after 3 weeks. Similar significant differences were observed when comparing the combined effect experimental group with the control group after 3 weeks. The combined treatment group exhibited 27·9 % lower serum LDL-cholesterol concentrations.

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The effect of coffee consumption on serum total cholesterol in the Sami and Norwegian populations

Public Health Nutrition ◽

10.1017/s1368980010000376 ◽

2010 ◽

Vol 13 (11) ◽

pp. 1818-1825 ◽

Cited By ~ 7

Author(s):

Tove Nystad ◽

Marita Melhus ◽

Magritt Brustad ◽

Eiliv Lund

Keyword(s):

Total Cholesterol ◽

Serum Cholesterol ◽

Coffee Consumption ◽

Cross Sectional Study ◽

Total Serum ◽

Total Serum Cholesterol ◽

Cross Sectional ◽

Cholesterol Levels ◽

The Mean ◽

The Impact

AbstractObjectiveTo assess coffee consumption in the Sami and Norwegian populations and to investigate the impact of unfiltered boiled coffee consumption on serum cholesterol concentrations.DesignA cross-sectional study. Information was collected by self-administrated questionnaires and total serum cholesterol was analysed. Participants were divided into three ethnic groups: Sami I (Sami used as home language in the last three generations), Sami II (at least one Sami identity marker) and Norwegian.SettingIn an area with Sami, Kven/Finnish and Norwegian populations, the SAMINOR study, 2003–2004.SubjectsA total of 5647 men and 6347 women aged 36–79 years.ResultsMore than 90 % of the study populations were coffee drinkers. Only 22 % were unfiltered coffee consumers. Sami I had the highest proportion of participants who consumed nine or more cups of unfiltered coffee per day, although the number of participants was limited. Total coffee consumption was associated with increased total cholesterol for men (P < 0·01) and women (P < 0·0001). For those who drank only unfiltered coffee, a significant association was found only in Norwegian men, adjusted for physical activity in leisure time, BMI and smoking habits (P < 0·001). From the lowest (less than five cups) to the highest (nine or more cups) unfiltered coffee consumption category, the mean total cholesterol levels increased by 0·29 mmol/l in Norwegian men.ConclusionsUnfiltered coffee consumption was lower in the present study compared to previous reports. In general, total coffee consumption was positively associated with total cholesterol levels. However, for unfiltered coffee consumption, an association was found only in Norwegian men.

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