scholarly journals Biochemical and histopathological effects of carbendazim to rat male reproduction

Pesticidi ◽  
2002 ◽  
Vol 17 (1-2) ◽  
pp. 59-71 ◽  
Author(s):  
Nurhayat Barlas ◽  
Güldeniz Selmanoglu ◽  
Sibel Songür ◽  
Azru Koçkaya ◽  
Esra Erdemli
Keyword(s):  
Serum Testosterone ◽  
Male Reproduction ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Total Serum ◽  
Seminiferous Tubules ◽  
Total Testosterone ◽  
Electron Microscopic ◽  
Hormone Levels ◽  
Testis Tissue

The purpose of this study was to determine the effects of carbendazim (methyl 2-benzimidazole carbamate) on the some hormone levels and on testis tissue. Carbendazim in the doses of 0, 150, 300, and 600 mg/kg/day were administered (by gavage) to male rats, daily for 15 weeks. At the end of the experiment serum total testosterone and dihydrotestosterone (DHT) levels were analyzed and testis tissues were taken for light and electron microscopic examinations. A statistically significant decrease in total serum testosterone level in rats exposed to carbendazim (dose: 300 and 600 mg/kg/day), compared to the control, was observed. Also, dihydrotestosterone level was significantly decreased in all experimental groups. Histopathologically, carbendazim caused detrimental effects in testis tissues. These effects were vacuolization, disorganization and necrosis in germinal epithelium. In addition, multinucleated giant cells were observed in the lumen of the seminiferous tubules. These pathological findings were supported by electron microscopic examinations. These results suggest that carbendazim, in subchronic exposure, affects testis tissue and androgenic hormone levels.

scholarly journals Cichorium intybus L. extract ameliorates testicular oxidative stress induced by lead acetate in male rats

2020 ◽  
Vol 47 (3) ◽  
pp. 161-167
Author(s):  
Mehran Dorostghoal ◽  
Seyyed Mansour Seyyednejad ◽  
Marzieh Noroozi Tabrizi Nejad
Keyword(s):  
Oxidative Stress ◽  
Adverse Effects ◽  
Lead Acetate ◽  
Ethanolic Extract ◽  
Cichorium Intybus ◽  
Male Reproduction ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Testis Weight

Objective: Oxidative stress has been suggested as a possible mechanism for the adverse effects of heavy metal toxicity on male reproduction. Cichorium intybus L. is used in Iranian folk medicine as a hepatoprotective agent as well as for its supposed fertility-enhancing properties. The present study was performed to investigate whether the ethanolic extract of C. intybus leaves could protect male rats against lead-induced testicular oxidative stress.Methods: In this experimental study, adult Wistar rats were treated with 0.1% lead acetate in drinking water alone or with 50, 100, or 200 mg/ kg body weight of C. intybus extract via gavage once daily for 70 days. The weight of their reproductive organs, levels of serum hormones, histometric parameters of the seminiferous tubules, epidydimal sperm quality, and oxidative stress status were evaluated.Results: The testis weight, seminiferous tubule diameter, epididymal sperm count, serum testosterone level, and testicular levels of superoxide dismutase and glutathione peroxidase were significantly reduced (p<0.05) in the lead-treated rats. Moreover, significantly (p<0.05) higher levels of malondialdehyde were observed in the lead-exposed group compared to the control. However, the co-administration of C. intybus ethanolic extract in lead-treated rats was associated with a significant improvement in reproductive parameters.Conclusion: We conclude that C. intybus leaf extract has the potential to prevent lead-induced testicular toxicity and to suppress the adverse effects of lead on male reproductive health.

Estimations of total serum testosterone levels in Nigerian term neonates at birth using anogenital distance measurements

2020 ◽  
Vol 33 (5) ◽  
pp. 631-638
Author(s):  
Abiodun John Kareem ◽  
Joshua Aderinsola Owa ◽  
Jerome Boluwaji Elutayo Elusiyan
Keyword(s):  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Total Serum ◽  
Total Testosterone ◽  
Postnatal Life ◽  
Distance Measurements ◽  
Cross Sectional ◽  
Anogenital Distance ◽  
Term Neonates ◽  
Testosterone Levels

AbstractBackgroundIn genital anomalies, measurement of total testosterone is required but is expensive and technically difficult to assay. Therefore, the measurement of anogenital distance, which is non-invasive and cheap, could be used to estimate total serum testosterone in neonates. The objective if this study is to determine the relationship between total serum testosterone and anogenital distance and estimate total serum testosterone levels in term neonates using measurements of anogenital distance.MethodsThis was a prospective cross-sectional study. Consecutive healthy term neonates were recruited in the first 72 h of postnatal life. Anogenital distance was measured with a digital vernier calliper. Total serum testosterone was determined using enzyme linked immunoassay.ResultsA total of 240 term neonates comprising 124 (51.7%) males and 116 (48.3%) females were studied. The overall mean anogenital distance was 19.7 (7.7) mm and 26.5 (3.7) mm for males which was more than twice 12.4 (2.3) mm for females (t = 35.3, p < 0.001, 95% confidence interval [CI], 13–14). The overall mean total serum testosterone level was 267.1 (204.8) ng/dL; and 357.4 (241.7) ng/dL in males which was more than twice of 170.6 (80.7) ng/dL for females (t = 7.9, p < 0.001, 95% CI, 144–221). There was positive correlation between total serum testosterone and anogenital distance (r = 0.425, p < 0.001). The correlation was stronger in males than in females. The linear regression equation was as follows: total serum testosterone (ng/dL) = 44.3 + 11.3*AGD (mm) with 95% CI, 8–14.ConclusionsThe known value of anogenital distance could be used to estimate total serum testosterone levels in term neonates.

scholarly journals Effects of curcumin on sperm parameters abnormalities induced by morphine in rat

2017 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
S.H. Roshankhah ◽  
M.R. Salahshoor ◽  
S. Aryanfar ◽  
F. Jalili ◽  
M. Sohrabil ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Testosterone Level ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Treated Group ◽  
Sperm Parameters ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group

Opioids are the most potent and effective analgesics available and have become accepted as appropriate treatment for acute, cancer and non-cancer. Morphine, which is commonly used for the treatment of severe pain, gastrointestinal tract and kidneys. Curcumin petals consist of, glycosides, flavonoids, and anthocyanin. The study aims at evaluating curcumin effect and morphine on sperm parameters, testis tissue and serum testosterone level in rat. In this experimental study, 48 male rats with 28 weeks of age and limited weight of 270 to 300g were selected. They were divided into eight groups of 6, untreated control group; morphine – treated group (20 mg/kg/day); curcumin -treated groups (10, 30, 60 mg/kg/day); and morphine and curcumin treated group intraperitoneal administration for successive 28 days. After 24hours animals were killed. Sperm motility was measured using WHO protocols. The sperm parameter such as motility, sperm count, morphology, seminiferous tubules diameter, weight testis, and serum testosterone level were analyzed (oneway ANOVA). Curcumin (10, 30 and 60 mg/kg) significantly increased mean percentage of sperm motility, count, testis weight, and serum testosterone level compared to control group (p<0.05). Testosterone level decreased significantly in rats treated with morphine. Co-administration of curcumin to morphine-treated rats improved the histopathological alterations induced by morphine in testis and increased the sperm count. Curcumin has a very strong antioxidant effects at applied doses and it can probably be used as an antioxidant and food supplement in reproductive disorders.Journal of Medical and Biomedical Sciences (2017) 6(2), 1-10

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

scholarly journals Aphrodisiac Activity of Eulophia macrobulbon Extract on Erectile Dysfunction in Male Aged Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Watcharaporn Preedapirom ◽  
Kanokwan Changwichit ◽  
Piyarat Srisawang ◽  
Kornkanok Ingkaninan ◽  
Pornnarin Taepavarapruk
Keyword(s):  
Erectile Dysfunction ◽  
Serum Testosterone ◽  
Cyclic Guanosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Sildenafil Citrate ◽  
Male Rats ◽  
Cgmp Level ◽  
Seminiferous Tubules ◽  
Aged Rats ◽  
Sexual Performance

This study investigated the effect of Eulophia macrobulbon (EM) extract on sexual performance in aged-related erectile dysfunction (ED) rats. The ethanol EM extract at the doses of 15, 150, and 450 and sildenafil citrate at the dose of 5 mg/kg body weight (BW) were administered orally to the aged male rats once daily for 21 days. Mating parameters and intracavernosal pressure (ICP) were measured to evaluate their sexual and erection functions. Numbers of sperm and sperm motility as well as the diameter of seminiferous tubules were observed. The serum testosterone and 3’,5’-cyclic guanosine monophosphate (cGMP) concentration in the rat penile tissue were analyzed. The results showed the significant increased sexual motivation, copulatory performance, and ICP of aged rats treated with sildenafil citrate and all doses of EM extract as compared to control aged rats. Moreover, their serum testosterone levels were slightly increased and significant increase in penile cGMP concentration was observed in these aged rats treated with sildenafil citrate and EM extract. The results suggest that treatment with EM could inhibit activity of PDE5 in penile tissue resulting in the increased cGMP level and bring to the improvement of erectile function and sexual performance.

Decrease in serum testosterone levels after short-term occupational exposure to diisononyl phthalate in male workers

2020 ◽  
Vol 77 (4) ◽  
pp. 214-222 ◽  
Author(s):  
Jean-Bernard Henrotin ◽  
Eva Feigerlova ◽  
Alain Robert ◽  
Mathieu Dziurla ◽  
Manuela Burgart ◽  
...  
Keyword(s):  
Occupational Exposure ◽  
Serum Testosterone ◽  
Total Serum ◽  
Total Testosterone ◽  
Type I ◽  
Short Term ◽  
Testosterone Levels ◽  
Short Term Exposure ◽  
Diisononyl Phthalate ◽  
Male Workers

ObjectiveThe objective of the study was to examine the effects of occupational exposure to diisononyl phthalate (DINP) on serum testosterone levels in male workers.MethodsFrom 2015 to 2018, 97 male workers were recruited from six French factories in the plastics industry. In a short longitudinal study, changes over 3 days in the level of total or free serum testosterone and DINP exposure were measured. DINP exposure was measured by urinary biomonitoring: mono-4-methyl-7-oxo-octyl phthalate (OXO-MINP), mono-4-methyl-7-hydroxy-octyl phthalate (OH-MINP) and mono-4-methyl-7-carboxyheptylphthalate (CX-MINP). We further analysed changes in follicle-stimulating hormone, luteinising hormone, total testosterone to oestradiol ratio and two bone turnover markers (procollagen-type-I-N propeptide, C terminal cross-linking telopeptide of type I collagen), and erectile dysfunction via standardised questionnaires (International Index of Erectile Function, Androgen Deficiency in Aging Males). Linear mixed models were used with the variables ‘age’ and ‘abdominal diameter’ included as confounder.ResultsIncreased urinary OXO-MINP was associated with a significant decrease in total serum testosterone concentrations, but only for workers who exhibited the smallest variations and lowest exposures (p=0.002). The same pattern was observed for CX-MINP but was not significant; no association with OH-MINP was detectable. More self-reported erectile problems were found in workers exposed directly to DINP at the workstation (p=0.01). No changes were observed for the other biological parameters.ConclusionsShort-term exposure to DINP is associated with a decrease in total serum testosterone levels in male workers. Our results suggest that DINP could present weak antiandrogenic properties in humans, but these need to be confirmed by other studies.

Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat

2007 ◽  
Vol 23 (7) ◽  
pp. 439-444 ◽  
Author(s):  
Suresh C Joshi ◽  
R. Mathur ◽  
N. Gulati
Keyword(s):  
Histopathological Examination ◽  
Sperm Count ◽  
The Body ◽  
Male Reproduction ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Albino Rat ◽  
Toxic Activity ◽  
Sperm Counts ◽  
Dose Levels

Organophosphates are among the most widely used synthetic insect pesticides. The widespread use of organophosphates has stimulated research into the possible existence of effects related with their reproductive toxic activity. Present study was therefore, undertaken to assess the effects of chlorpyrifos on testes, the main organ of male reproduction. Chlorpyrifos at the dose levels of 7.5, 12.5 and 17.5 mg/kg b. wt./day was administered orally to male rats of Wistar strain for 30 days to evaluate the toxic alterations in testicular histology, biochemistry, sperm dynamics and testosterone levels. The body weight of animals did not show any significant change, however, a significant reduction was observed in testes. Chlorpyrifos also brought about marked reduction in epididymal and testicular sperm counts in exposed males and a decrease in serum testosterone concentration. Histopathological examination of testes showed mild to severe degenerative changes in seminiferous tubules at various dose levels. Fertility test showed 85% negative results. A significant reduction in the sialic acid content of testes and testicular glycogen was noticed, whereas the protein and cholesterol content was raised at significant levels. All these toxic effects are moderate at low doses and become severe at higher dose levels. From the results of the present study it is concluded that chlorpyrifos induces severe testicular damage and results in reduction in sperm count and thus affect fertility. Small changes in sperm counts are known to have adverse affects on human fertility. Therefore, application of such insecticide should be limited to a designed programme. Toxicology and Industrial Health 2007; 23: 439—444.

Evaluation of the reproductive toxicity of naproxen sodium and meloxicam in male rats

2014 ◽  
Vol 34 (4) ◽  
pp. 415-429 ◽  
Author(s):  
B Uzun ◽  
O Atli ◽  
BO Perk ◽  
D Burukoglu ◽  
S Ilgin
Keyword(s):  
Naproxen Sodium ◽  
Histopathological Examination ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Prostaglandin E ◽  
Cox 2 ◽  
Cox 1 ◽  
Testis Tissue

Nonsteroidal anti-inflammatory drugs that are cyclooxygenase (COX) enzyme inhibitors have generally been used in short-term pain management and also to treat inflammation chronically. It is known that COX enzyme and prostaglandins play important roles in the regulation of reproductive functions in females. However, there are relatively few studies for the male reproductive system, and the results of these studies are contradictory. In this study, sperm count and motility, COX-1, COX-2, prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), and prostaglandin F2α (PGF2α) levels in testis tissue, plasma follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels, and histopathological examination of testis tissue were evaluated after naproxen sodium and meloxicam administration in male rats. Also, testis superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione (GSH) levels were measured to investigate the oxidation status. According to our results, sperm count and motility were significantly decreased in treatment groups. Plasma hormone levels did not show any statistical differences between the groups. COX-1, PGE2, and PGF2α levels were significantly decreased, while the decreases in COX-2 and PGE1 levels did not show any significance statistically. Testis SOD, catalase, GPx, and GSH levels were decreased significantly. According to the results of histopathological examination, damage in seminiferous tubules, where spermatogenesis developed, was observed. In conclusion, naproxen sodium and meloxicam decreased the sperm count and motility and also induced the damage of seminiferous tubules as a direct effect without affecting plasma hormone levels in our study. The mechanism of the reproductive toxicity induced by these agents may be based on the inhibition of prostaglandin synthesis and the induction of oxidative stress can be emphasized as a secondary factor.

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