scholarly journals Cichorium intybus L. extract ameliorates testicular oxidative stress induced by lead acetate in male rats

2020 ◽  
Vol 47 (3) ◽  
pp. 161-167
Author(s):  
Mehran Dorostghoal ◽  
Seyyed Mansour Seyyednejad ◽  
Marzieh Noroozi Tabrizi Nejad
Keyword(s):  
Oxidative Stress ◽  
Adverse Effects ◽  
Lead Acetate ◽  
Ethanolic Extract ◽  
Cichorium Intybus ◽  
Male Reproduction ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Testis Weight

Objective: Oxidative stress has been suggested as a possible mechanism for the adverse effects of heavy metal toxicity on male reproduction. Cichorium intybus L. is used in Iranian folk medicine as a hepatoprotective agent as well as for its supposed fertility-enhancing properties. The present study was performed to investigate whether the ethanolic extract of C. intybus leaves could protect male rats against lead-induced testicular oxidative stress.Methods: In this experimental study, adult Wistar rats were treated with 0.1% lead acetate in drinking water alone or with 50, 100, or 200 mg/ kg body weight of C. intybus extract via gavage once daily for 70 days. The weight of their reproductive organs, levels of serum hormones, histometric parameters of the seminiferous tubules, epidydimal sperm quality, and oxidative stress status were evaluated.Results: The testis weight, seminiferous tubule diameter, epididymal sperm count, serum testosterone level, and testicular levels of superoxide dismutase and glutathione peroxidase were significantly reduced (p<0.05) in the lead-treated rats. Moreover, significantly (p<0.05) higher levels of malondialdehyde were observed in the lead-exposed group compared to the control. However, the co-administration of C. intybus ethanolic extract in lead-treated rats was associated with a significant improvement in reproductive parameters.Conclusion: We conclude that C. intybus leaf extract has the potential to prevent lead-induced testicular toxicity and to suppress the adverse effects of lead on male reproductive health.

scholarly journals Biochemical and histopathological effects of carbendazim to rat male reproduction

Pesticidi ◽  
2002 ◽  
Vol 17 (1-2) ◽  
pp. 59-71 ◽  
Author(s):  
Nurhayat Barlas ◽  
Güldeniz Selmanoglu ◽  
Sibel Songür ◽  
Azru Koçkaya ◽  
Esra Erdemli
Keyword(s):  
Serum Testosterone ◽  
Male Reproduction ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Total Serum ◽  
Seminiferous Tubules ◽  
Total Testosterone ◽  
Electron Microscopic ◽  
Hormone Levels ◽  
Testis Tissue

The purpose of this study was to determine the effects of carbendazim (methyl 2-benzimidazole carbamate) on the some hormone levels and on testis tissue. Carbendazim in the doses of 0, 150, 300, and 600 mg/kg/day were administered (by gavage) to male rats, daily for 15 weeks. At the end of the experiment serum total testosterone and dihydrotestosterone (DHT) levels were analyzed and testis tissues were taken for light and electron microscopic examinations. A statistically significant decrease in total serum testosterone level in rats exposed to carbendazim (dose: 300 and 600 mg/kg/day), compared to the control, was observed. Also, dihydrotestosterone level was significantly decreased in all experimental groups. Histopathologically, carbendazim caused detrimental effects in testis tissues. These effects were vacuolization, disorganization and necrosis in germinal epithelium. In addition, multinucleated giant cells were observed in the lumen of the seminiferous tubules. These pathological findings were supported by electron microscopic examinations. These results suggest that carbendazim, in subchronic exposure, affects testis tissue and androgenic hormone levels.

Ethyl acetate fraction of Spathodea campanulata (Bignoniaceae) attenuates lead acetate induced testicular toxicity in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Glory Olubusayo Adesiyan ◽  
Temitope Olaoluwa Talabi ◽  
Oluwafemi Ezekiel Kale ◽  
Temitope Funmi Ogundare
Keyword(s):  
Ethyl Acetate ◽  
Lead Acetate ◽  
Octadecenoic Acid ◽  
Morphological Characteristics ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Male Rats ◽  
Bark Extract ◽  
Seminiferous Tubules ◽  
Testicular Toxicity

Abstract Objectives The use of Spathodea campanulata in folklore medicine for the management of reproductive disorders has been poorly reported. We sought to investigate the protective potential of the ethyl acetate fraction of S. campanulata stem bark extract (EFSC) on lead acetate-induced (LA) testicular toxicity in male rats. Methods Animals during a 28 days treatment received dimethyl sulfoxide (DMSO, 0.1%), LA (20 mg/kg), and EFSC (200 mg/kg). Others received EFSC only (100, 200, and 400 mg/kg) or vitamin E (100 mg/kg) 1 h prior to LA (20 mg/kg) administration. Results LA administration decreased sperm counts and motility by 36.39 and 40.69% respectively in rats. Also, LA-untreated rats showed elevated malondialdehyde (MDA) and decreased total proteins in testis (260, 33%) and epididymis (62, 29%) respectively. However, EFSC (100, 200, or 400 mg/kg) administrations improved sperm morphological characteristics as well as antioxidant status in LA-treated rats. EFSC (400 mg/kg) showed improved testis seminiferous tubules that were almost normal in the LA-treated rats. Further, EFSC contains a high 9-octadecenoic acid methyl ester. Conclusions Overall, evidence by LA-induced testicular toxicity, EFSC provides chemopreventive roles via antioxidant mechanisms.

Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat

2007 ◽  
Vol 23 (7) ◽  
pp. 439-444 ◽  
Author(s):  
Suresh C Joshi ◽  
R. Mathur ◽  
N. Gulati
Keyword(s):  
Histopathological Examination ◽  
Sperm Count ◽  
The Body ◽  
Male Reproduction ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Albino Rat ◽  
Toxic Activity ◽  
Sperm Counts ◽  
Dose Levels

Organophosphates are among the most widely used synthetic insect pesticides. The widespread use of organophosphates has stimulated research into the possible existence of effects related with their reproductive toxic activity. Present study was therefore, undertaken to assess the effects of chlorpyrifos on testes, the main organ of male reproduction. Chlorpyrifos at the dose levels of 7.5, 12.5 and 17.5 mg/kg b. wt./day was administered orally to male rats of Wistar strain for 30 days to evaluate the toxic alterations in testicular histology, biochemistry, sperm dynamics and testosterone levels. The body weight of animals did not show any significant change, however, a significant reduction was observed in testes. Chlorpyrifos also brought about marked reduction in epididymal and testicular sperm counts in exposed males and a decrease in serum testosterone concentration. Histopathological examination of testes showed mild to severe degenerative changes in seminiferous tubules at various dose levels. Fertility test showed 85% negative results. A significant reduction in the sialic acid content of testes and testicular glycogen was noticed, whereas the protein and cholesterol content was raised at significant levels. All these toxic effects are moderate at low doses and become severe at higher dose levels. From the results of the present study it is concluded that chlorpyrifos induces severe testicular damage and results in reduction in sperm count and thus affect fertility. Small changes in sperm counts are known to have adverse affects on human fertility. Therefore, application of such insecticide should be limited to a designed programme. Toxicology and Industrial Health 2007; 23: 439—444.

scholarly journals Chitosan/Selenium Nanoparticles Attenuate Diclofenac Sodium-Induced Testicular Toxicity in Male Rats

Crystals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1477
Author(s):  
Samy M. El-Megharbel ◽  
Fawziah A. Al-Salmi ◽  
Sarah Al-Harthi ◽  
Khadeejah Alsolami ◽  
Reham Z. Hamza
Keyword(s):  
Oxidative Stress ◽  
Diclofenac Sodium ◽  
Chitosan Nanoparticles ◽  
Reproductive Toxicity ◽  
Male Reproduction ◽  
Male Rats ◽  
Antioxidant Enzyme Activities ◽  
Testicular Function ◽  
Wide Range ◽  
The Impact

The detrimental effect of diclofenac sodium (Diclo-Na) on male reproductive organs is reported upon in this paper. Chitosan is a polysaccharide composed of various amounts of glucosamine. Chitosan nanoparticles (CH-NPs) have attracted much attention owing to their biomedical activity. Selenium (Se) has a vital role in nutrition, plays an important role in enhancing male reproduction, and has a wide range of free radical scavenging activities. However, the study of the impact of chitosan nanoparticles in combination with Se (IV) (CH-NPs/Se) on male reproductive toxicity associated with Diclo-Na administration is lacking in recent literature. The current study assessed the ameliorative effects of complexes of CH-NPs/Se (IV) on Diclo-Na and the ways in which they alter reproductive toxicity in male rats. Male rats were treated for 30 days successively, either with Diclo-Na (10 mg/kg) or co-treated with a CH-NPs/Se complex (280 mg/kg). Sperm characteristics, marker enzymes of testicular function, LH, FSH, and testosterone were evaluated in addition to oxidative stress markers and histological alterations. CH-NPs/Se significantly alleviated Diclo-Na-induced decline in sperm count and motility, testicular function enzymes, and levels of LH and testosterone in serum. Additionally, CH-NPs/Se co-administration at 280 mg/Kg, inhibited the Diclo-Na-induced decline of antioxidant enzyme activities and elevated oxidative stress indices and reactive free radicals in testicular homogenates of male rats. CH-NPs/Se (280 mg/kg) alone improved Diclo-Na and ameliorated histological damages in exposed rats. In conclusion, chitosan improved testicular function in Diclo-Na-treated rats by enhancing the testosterone hormone levels, ameliorating testicular tissue, and inhibiting markers of oxidative stress in male rats.

scholarly journals Pyrethroids Toxicity to Male Reproductive System and Offspring as a Function of Oxidative Stress Induction: Rodent Studies

2021 ◽  
Vol 12 ◽  
Author(s):  
Xu Zhang ◽  
Tongtong Zhang ◽  
Xiaohan Ren ◽  
Xinglin Chen ◽  
ShangQian Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Motility ◽  
Reproductive System ◽  
Sperm Quality ◽  
Sperm Count ◽  
Meta Analysis ◽  
Serum Testosterone ◽  
Male Reproductive System ◽  
Serum Testosterone Level ◽  
Testis Weight

Pyrethroids may be related to male reproductive system damage. However, the results of many previous studies are contradictory and uncertain. Therefore, a systematic review and a meta-analysis were performed to assess the relationship between pyrethroid exposure and male reproductive system damage. A total of 72 articles were identified, among which 57 were selected for meta-analysis, and 15 were selected for qualitative analysis. Pyrethroid exposure affected sperm count (SMD= -2.0424; 95% CI, -2.4699 to -1.6149), sperm motility (SMD=-3.606; 95% CI, -4.5172 to -2.6948), sperm morphology (SMD=2.686; 95% CI, 1.9744 to 3.3976), testis weight (SMD=-1.1591; 95% CI, -1.6145 to -0.7038), epididymal weight (SMD=-1.1576; 95% CI, -1.7455 to -0.5697), and serum testosterone level (SMD=-1.9194; 95% CI, -2.4589 to -1.3798) in the studies of rats. We found that gestational and lactational exposure to pyrethroids can reduce sperm count (SMD=1.8469; 95% CI, -2.9010 to -0.7927), sperm motility (SMD=-2.7151; 95% CI, -3.9574 to -1.4728), testis weight (SMD=-1.4361; 95% CI, -1.8873 to -0.9848), and epididymal weight (SMD=-0.6639; 95% CI, -0.9544 to -0.3733) of F1 offspring. Exposure to pyrethroids can increase malondialdehyde (SMD=3.3451; 95% CI 1.9914 to 4.6988) oxide in testes and can reduce the activities of glutathione (SMD=-2.075; 95% CI -3.0651 to -1.0848), superoxide dismutase (SMD=-2.4856; 95% CI -3.9612 to -1.0100), and catalase (SMD=-2.7564; 95% CI -3.9788 to -1.5340). Pyrethroid exposure and oxidative stress could damage male sperm quality. Gestational and lactational pyrethroid exposure affects the reproductive system of F1 offspring.

Di(2-ethylhexyl)phthalate induces reproductive toxicity via JAZF1/TR4 pathway and oxidative stress in pubertal male rats

2019 ◽  
Vol 35 (3) ◽  
pp. 228-238 ◽  
Author(s):  
Yu-Qin Shi ◽  
Guo-Qing Fu ◽  
Jing Zhao ◽  
Shen-Zhou Cheng ◽  
You Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Morphological Changes ◽  
Reproductive Toxicity ◽  
Male Reproduction ◽  
Male Rats ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Cyclin A1 ◽  
Ethylhexyl Phthalate ◽  
Dehp Exposure ◽  
And Oxidative Stress

Di(2-ethylhexyl)phthalate (DEHP) is a typical endocrine-disrupting chemical and reproductive toxicant. Although previous studies have attempted to describe the mechanism by which DEHP exposure results in reproductive dysfunction, few studies focused on puberty, a critical period of reproductive development, and the increased susceptibility to injury in adolescents. To elucidate the mechanism underpinning the testicular effects of DEHP in puberty, we sought to investigate the JAZF1/TR4 pathway in the testes of pubertal rats. Specifically, we focused on the role of the JAZF1/TR4 pathway in male reproduction, including the genes JAZF1, TR4, Sperm 1, and Cyclin A1. In the present study, rats were exposed to increasing concentrations of DEHP (0, 250, 500, and 1000 mg/kg/day) by oral gavages for 30 days. Then we assayed testicular zinc and oxidative stress levels. Our results indicated that DEHP exposure could lead to oxidative stress and decrease the contents of testicular zinc. Additionally, significant morphological changes and cell apoptosis were observed in testes exposed to DEHP, as identified by hematoxylin and eosin staining and the terminal deoxynucleotidyl transferase-mediated nick and labeling assay. By measuring the expression levels of the above relevant genes by qPCR, we found the DEHP-induced increased expression of JAZF1 and decreased expression of TR4, Sperm 1, and Cyclin A1. Therefore, we have demonstrated that in vivo exposure to DEHP might induce reproductive toxicity in pubertal male rats through the JAZF1/TR4 pathway and oxidative stress.

Cytoprotective Effects of Zingiber officinale Against the Oxidative Stress Induced by Lead Acetate Toxicity in Rats

2020 ◽  
Author(s):  
O. Aouacheri ◽  
S. Saka
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Lead Acetate ◽  
Zingiber Officinale ◽  
Treated Group ◽  
Normal Diet ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diet ◽  
Group I

The evaluation of the effect of ginger on the modulation of toxic effects induced by lead is the objective of our study. Forty male rats were randomly divided into four groups and treated daily for 3 consecutive months. Group I (0-0) was kept as control; group II (0-G) received an experimental diet with 2% of ginger; group III (Pb-0) received 2% lead acetate dissolved in drinking water with a normal diet; and group IV (Pb-G) received 2% lead acetate in drinking water and an experimental diet containing 2% ginger. Lead acetate exposure caused a significant increase of organosomatic indexes, hepatic, lipid, and urine profiles. In addition, lead acetate has a pro-oxidative effect expressed by a significant decrease in tissue GSH levels and the enzymatic activity of GPx and CAT. This pro-oxidative action was also marked by an increase in MDA level and GST activity in lead-treated group. Feeding ginger-supplemented diet to lead acetate-treated rats restored all the parameters studied as compared to control. These results suggest that ginger treatment exerts a protective effect on metabolic disorders by decreasing the oxidative stress.

Effects of subchronic exposure to carbendazim on spermatogenesis and fertility in male rats

2009 ◽  
Vol 25 (1) ◽  
pp. 41-47 ◽  
Author(s):  
G Yu ◽  
Q Guo ◽  
L Xie ◽  
Y Liu ◽  
X Wang
Keyword(s):  
Germ Cells ◽  
Flow Cytometric Analysis ◽  
Testicular Tissue ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Testis Weight ◽  
Subchronic Exposure ◽  
Flow Cytometric ◽  
Sperm Counts

The aim of this study is to investigate the effects of subchronic exposure to carbendazim on spermatogenesis and fertility in male rats. Ninety-eight healthy male rats were divided into four groups: three exposure groups and a control group. Carbendazim was administered orally to male rats at 0, 20, 100 and 200 mg/kg for 80 days prior to mating. Each male was cohabited with an unexposed female for a maximum of 5 days. In 100 and 200 mg/kg groups, the mating index was relatively increased, the fertility index was decreased, and the testis weight, the sperm counts and motility were also decreased. The levels of luteinizing hormone (LH) showed a decreasing tendency and there was a statistical difference between the 200 mg/kg group and the control group. There were no obvious effects on the levels of follicle stimulating hormone (FSH) and testosterone (T). Histopathological evaluation showed atrophic seminiferous tubules, decreased germ cells, and increased sloughing of germ cells. Flow cytometric analysis of the testicular tissue revealed that carbendazim inhibited meiotic transformation and interfered with the spermatogenic process. These results suggest that carbendazim has adverse effects on spermatogenesis, resulting in reduced fertility in male rats.

scholarly journals Overexpression of miR-506-3p Aggravates DBP-Induced Testicular Oxidative Stress in Rats by Downregulating ANXA5 via Nrf2/HO-1 Signaling Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Min Tang ◽  
Lei Zhang ◽  
Zheng Zhu ◽  
Ran Li ◽  
Shangqian Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Male Rats ◽  
Adolescent Male ◽  
Seminiferous Tubules ◽  
Stress Injury ◽  
Oxidative Stress Injury ◽  
Testicular Injury

Background. Di-N-butylphthalate (DBP) is a kind of unique endocrine toxicity linked to hormonal disruptions that affects the male reproductive system and has given rise to more and more attention. However, the mechanism of DBP-induced testicular injury remains unclear. Here, the objective of this study was to investigate the potential molecular mechanism of miR-506-3p in DBP-induced rat testicular oxidative stress injury via ANXA5 (Annexin A5)/Nrf2/HO-1 signaling pathway. Methods. In vivo, a total of 40 adolescent male rats were treated from 2 weeks with 800 mg/kg/day of DBP in 1 mL/kg corn oil administered daily by oral gavage. Among them, some rats were also injected subcutaneously with 2 nmol agomir-506-3p and/or 10 nmol recombinant rat ANXA5. The pathomorphological changes of testicular tissue were assessed by histological examination, and the antioxidant factors were evaluated. Subsequently, ANXA5, Nrf2, and its dependent antioxidant enzymes, such as HO-1, NQO1, and GST, were detected by Western blotting or immunohistochemical staining. In vitro, TM3 cells (Leydig cells) were used to detect the cell activity by CCK-8 and the transfection in the DBP-treated group. Results. Differentially expressed miRNAs between the DBP-treated and normal rats were analyzed, and qRT-PCR showed miR-506-3p was highly expressed in testicular tissues of the DBP-treated rats. DBP-treated rats presented severe inflammatory infiltration, increased abnormal germ cells, and missed cell layers frequently existed in seminiferous tubules, resulted in oxidative stress and decreased testicular function. Meanwhile, upregulation of miR-506-3p aggravated the above changes. In addition, miR-506-3p directly bound to ANXA5, and overexpression of miR-506-3p could reduce the ANXA5 expression and also decrease the protein levels of Nrf2/HO-1 signaling pathway. Additionally, we found that recombinant rat ANXA5 reversed the DBP-treated testicular oxidative stress promoting injury of miR-506-3p in rats. In vivo results were reproduced in in vitro experiments. Conclusions. This study provided evidence that miR-506-3p could aggravate the DBP-treated testicular oxidative stress injury in vivo and in vitro by inhibiting ANXA5 expression and downregulating Nrf2/HO-1 signaling pathway, which might provide novel understanding in DBP-induced testicular injury therapy.

scholarly journals Potential therapeutic effect of turmeric (Curcuma longa) against adverse effects of penconazole fungicide to white rats

2016 ◽  
Vol 4 (2) ◽  
pp. 178 ◽  
Author(s):  
Mona Abdel Rasoul ◽  
Gehan Marei
Keyword(s):  
Adverse Effects ◽  
Curcuma Longa ◽  
Lethal Dose ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Gamma Glutamyl Transferase ◽  
White Rats ◽  
Testicular Weight ◽  
Liver And Kidney ◽  
Glutamyl Transferase

This study aimed to investigate the prophylactic effect of turmeric (Curcuma longa) Rhizome Ethanolic extract (CLRE) at 250 mg/kg as antioxidant effects against penconazole induced sub-acute toxicity. Hepatic, renal and testicular pathological changes caused by oxidative damage induced by penconazole in rats were biochemically and histologically evaluated. Male rats were treated with penconazole, via oral route, at doses of 0.5 mg/ kg body weight (b.w.; acute reference dose, ARfD), 25 mg/kg b.w. (no observed adverse effects level, NOAEL) and 100 mg/ kg b.w. (1/20 lethal dose [LD50]) for 28 consecutive days. Penconazole treatments had significant (p < 0.05) and gradual reductions in body and relative testicular weight accompanied by significant elevation in the relative liver and kidney weights. Significant increase serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dhydrogenase(LDH), gamma-glutamyl transferase (GGT), creatinine (Cre), uric acid and blood glucose was observed due to penconazole treatments. However, total protein and testosterone hormone were significantly decreased. Exposure to penconazole caused increase in lipid peroxidation (LPO) and decreased of liver and kidney antioxidant enzymes activity as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx). Histopathological studies confirmed the ameliorative beneficial effects of turmeric biochemical parameters. On the basis of this study, the use of tumeric rhizomes as a functional food or as a nutraceutical product could be a useful approach to protect individuals who are regularly exposed to penconazole.

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