scholarly journals MicroRNA-34 expression in gingival crevicular fluid correlated with orthodontic tooth movement

2020 ◽  
Vol 90 (5) ◽  
pp. 702-706
Author(s):  
Bin Zhang ◽  
Li Yang ◽  
Weilong Zheng ◽  
Ting Lin
Keyword(s):  
Baseline Level ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Level 3 ◽  
Polymerase Chain ◽  
Crevicular Fluid

ABSTRACT Objectives To explore the expression of miR-34a and its effect on expression of matrix metalloproteinases (MMPs) during orthodontic tooth movement (OTM). Materials and Methods Twenty patients, age 12–18 years old, who underwent orthodontic treatment were enrolled. The expression of miR-34a and MMPs (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMP-14) were detected in gingival crevicular fluid by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction at different time points. The miR-34a mimics or inhibitors were transfected into human periodontal ligament (hPDL) cells, and the MMP expression was measured by ELISA. Results The miR-34 expression in GCF on both the tension and pressure sides after orthodontic treatment were significantly downregulated, while the levels of MMPs were significantly upregulated compared with baseline level. The levels of miR-34 and MMPs returned to baseline level 3 months after orthodontic treatment. The expression of miR-34 was negatively correlated with the expression of MMP-2, MMP-9, and MMP-14. After transfection with miR-34, the MMP-2, MMP-9, and MMP-14 expression by hPDL cells were significantly downregulated compared with miR-control and miR-34 inhibitor. Conclusions Downregulated miR-34 expression was positively correlated with MMP-2, MMP-9, and MMP-14 expression. The miR-34a transfection into hPDL cells inhibited expression of MMPs. The results suggest that miR-34a is involved in expression of MMPs during OTM.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

scholarly journals Vibratory stimulation increases interleukin-1 beta secretion during orthodontic tooth movement

2015 ◽  
Vol 86 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Chidchanok Leethanakul ◽  
Sumit Suamphan ◽  
Suwanna Jitpukdeebodintra ◽  
Udom Thongudomporn ◽  
Chairat Charoemratrote
Keyword(s):  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Orthodontic Tooth Movement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Orthodontic Force ◽  
Electric Toothbrush ◽  
Experimental Canine ◽  
Crevicular Fluid ◽  
Canine Distalization

ABSTRACT Objectives:  To investigate the effects of application of vibratory stimuli on interleukin (IL)–1β secretion during maxillary canine distalization. Materials and Methods:  Split-mouth design study in 15 subjects (mean age, 22.9 years; range 19–25 years) whose bilateral maxillary first premolars were extracted with subsequent canine distalization. On the experimental side, light force (60 g) was applied to the canine for 3 months in combination with vibratory stimuli provided using an electric toothbrush 15 minutes a day for 2 months; only orthodontic force was applied to the contralateral control canine. Gingival crevicular fluid (GCF) was collected from the mesial and distal sides of each canine at each monthly appointment. IL-1β levels were analyzed using an enzyme-linked immunosorbent assay. Canine movement was measured monthly. Results:  Overall, enhanced IL-1β secretion was observed at the pressure sites of experimental canines compared to control canines (mean, 0.64 ± 0.33 pg/µL vs 0.10 ± 0.11 pg/µL, respectively, P < .001). The accumulative amount of tooth movement was greater for the experimental canine than for the control canine (mean, 2.85 ± 0.17 mm vs 1.77 ± 0.11 mm, respectively, P < .001). Conclusions:  This study demonstrates that, in combination with light orthodontic force, application of vibratory stimuli using an electric toothbrush enhanced the secretion of IL-1β in GCF and accelerated orthodontic tooth movement.

scholarly journals Amide I Band Analysis Applied to Vibrational Micro-Spectroscopies of Gingival Crevicular Fluid Samples for Orthodontic Treatment Monitoring

2021 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Carlo Camerlingo ◽  
Ludovica Nucci ◽  
Fabrizia D’Apuzzo ◽  
Vincenzo Grassia ◽  
Letizia Perillo ◽  
...  
Keyword(s):  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Deconvolution Analysis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Vibrational Spectroscopies ◽  
The Individual ◽  
Crevicular Fluid

Vibrational micro-spectroscopies were applied to investigate the gingival crevicular fluid (GCF) for monitoring orthodontic treatment with fixed appliances. The GCF samples were investigated using Fourier transform infrared, Raman, and surface-enhanced Raman micro-spectroscopies. The GCF spectra collected at different times of orthodontic tooth movement were used to characterize the biochemical changes occurring during the treatment. We examined the amide I band region by means of deconvolution analysis using Gaussian–Lorentzian curves for infrared spectra and Lorentzian curves for Raman spectra. This analysis allowed us to evidence the contribution of the different subcomponents of the amide I band and the changes occurring during orthodontic treatment. These changes can be ascribed to modifications in the secondary structure of protein content and could contribute to make vibrational spectroscopies a useful tool for monitoring the individual patient’s response to orthodontic force application.

scholarly journals Effect of ovarian activity on orthodontic tooth movement and gingival crevicular fluid levels of interleukin-1β and prostaglandin E2 in cats

2012 ◽  
Vol 83 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Ahmet Arif Celebi ◽  
Serhat Demirer ◽  
Bulent Catalbas ◽  
Sevket Arikan
Keyword(s):  
Prostaglandin E2 ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Interleukin 1Β ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Activity ◽  
Coil Spring ◽  
Significant Difference ◽  
Crevicular Fluid

Abstract Objective: To evaluate whether there is any correlation between ovarian activity and two potent bone-resorbing mediators (prostaglandin E2 [PGE2], interleukin-1β [IL-1β]) secreted from the gingival crevicular fluid (GCF) during orthodontic tooth movement. Materials and Methods: Eighteen female cats were included in this study. Animals were randomly divided into three groups (estrous, anestrous, and ovariectomized groups), each having six queens. Estrous was induced by administration of 150 IU equine chorionic gonadotropin (eCG) to queens of the estrous group. A closed-coil spring, applied with 80 g of tipping force to the canine, was attached between the maxillary canine and mini-implant. GCF was collected on days 0, 6, and 12 from each cat to examine PGE2 and IL-1β during orthodontic tooth movement in cats. The PGE2 and IL-1β levels were determined with enzyme-linked immunosorbent assay. Results: There was no significant difference (P > .05) between anestrous and the ovariectomized groups in terms of tooth movement on days 6 and 12 of distalization. In contrast, tooth movement in the estrous group was lower (P < .05) than in the remaining two groups (anestrous and ovariectomized). The mean PGE2 and IL-1β levels of the canine teeth of the estrous groups were significantly lower than the remaining two groups on days 6 and 12 (P < .05) of coil spring applications. Conclusions: These results indicate that ovarian activity can affect orthodontic tooth movement and GCF levels of IL-1β and PGE2 in cats.

scholarly journals Biochemical markers of bone metabolism in gingival crevicular fluid during early orthodontic tooth movement

2012 ◽  
Vol 83 (1) ◽  
pp. 63-69 ◽  
Author(s):  
German Barbieri ◽  
Patricia Solano ◽  
Jose Antonio Alarcón ◽  
Rolando Vernal ◽  
Judith Rios-Lugo ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Transforming Growth Factor ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tension And Compression ◽  
Transforming Growth Factor ß1 ◽  
Crevicular Fluid ◽  
Orthodontic Forces

Abstract Objective: To evaluate the expression of an activator of nuclear factor-kappa (RANK), osteoprotegerin (OPG), osteopontin (OPN), and transforming growth factor ß1 (TGF-ß1) in gingival crevicular fluid (GCF) of teeth subjected to orthodontic forces. Materials and Methods: A randomized, pilot clinical trial including 10 healthy volunteers was conducted using a split-mouth design. Orthodontic elastic separators were placed between the second premolar and first molar, with the contralateral quadrant serving as a control. The GCF samples were collected from the tension and compression sites at baseline, 24 hours, and 7 days after the placement of separators. The GCF sample volumes were measured using a Periotron 8000, and total protein concentrations were determined. Levels of RANK, OPG, OPN, and TGF-ß1 were also analyzed using a multiplex enzyme-linked immunosorbent assay. Results: The control sites remained unchanged throughout the study. In contrast, the concentration of OPG significantly decreased at the compression site by 24 hours, and the amount and concentration of RANK differed significantly between the control, compression, and tension sites after 7 days. A significant increase in absolute TGF-ß1 levels was also detected at the compression site versus the control and tension sites after 7 days. Conclusion: Bone metabolism is affected by application of force to the teeth by elastic separators. Both increased expression of bone resorptive mediators (eg, RANK and TGF-ß1) and decreased expression of a bone-forming mediator (eg, OPG) on the compression side were detected.

scholarly journals Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

2021 ◽  
Vol 10 (7) ◽  
pp. 1405
Author(s):  
Fabrizia d’Apuzzo ◽  
Ludovica Nucci ◽  
Ines Delfino ◽  
Marianna Portaccio ◽  
Giuseppe Minervini ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
High Sensitivity ◽  
Data Analysis Procedure ◽  
Vibrational Spectroscopies ◽  
Composition Structure ◽  
The Individual ◽  
Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

scholarly journals Assessment of Aggregatibacter actinomycetemcomitans and Prevotella intermedia Counts around Healthy Implants, Diseased Implants and Sound Teeth: A Preliminary Study

2017 ◽  
Vol 9 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Siamak Yaghobee ◽  
Afshin Khorsand ◽  
Nojan Jahedmanesh ◽  
Mahdi Kadkhodazadeh
Keyword(s):  
Polymerase Chain Reaction ◽  
Gingival Crevicular Fluid ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Prevotella Intermedia ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Standard Curve ◽  
Preliminary Study ◽  
Polymerase Chain ◽  
Crevicular Fluid

Background. This study aimed to assess Aggregatibacter actinomycetemcomitans (Aa) and Prevotella intermedia (PI) counts in gingival crevicular fluid (GCF) around healthy implants, diseased implants and sound teeth. Methods. Eight patients (four males and four females), who had healthy implants, implants with peri-implantitis and sound teeth, were selected. Samples (GCF) were analyzed using real-time polymerase chain reaction (RT-PCR). The above-mentioned bacteria were detected and counted. Data analysis in RT-PCR was carried out based on the standard curve using Prism software to compare Pi and Aa counts between the three areas (GCF around sound teeth, healthy implants and implants with peri-implantitis). Results. Pi counts were significantly higher in GCF around implants with peri-implantitis (8 implants) than around healthy implants (8 implants) (P<0.001) and sound teeth (8) (P=0.012). No significant differences were found in Pi counts in GCF around healthy implants and sound teeth (P=0.063). Aa counts in GCF around implants with peri-implantitis were significantly higher than those around healthy implants (P=0.002) and sound teeth (P=0.024). No significant differences were noted in Aa counts in GCF around healthy implants and sound teeth (P=0.57). Conclusion. Aa and Pi counts in GCF around diseased implants were higher than around healthy implants and sound teeth. Also, Aa counts were significantly higher than Pi counts.

scholarly journals Highly variable rate of orthodontic tooth movement measured by a novel 3D method correlates with gingival inflammation

2020 ◽  
Author(s):  
Marc A. de Gouyon Matignon de Pontouraude ◽  
Johannes W. Von den Hoff ◽  
Frank Baan ◽  
Robin Bruggink ◽  
Marjon Bloemen ◽  
...  
Keyword(s):  
Biological Markers ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Contact Point ◽  
Anterior Segment ◽  
Three Dimensional ◽  
Orthodontic Tooth Movement ◽  
Large Individual ◽  
Gingival Inflammation

Abstract Objectives Individual orthodontic treatment duration is hard to predict. Individual biological factors are amongst factors influencing individual rate of orthodontically induced tooth movement (OTM). The study aim is to determine the rate of OTM by a novel 3D method and investigate parameters that may predict the rate of tooth movement. Materials and methods In this prospective cohort study, rate of OTM was determined from 90 three-dimensional intra-oral scans in 15 patients (aged 12–15) undergoing orthodontic treatment. For each patient, intra-oral scans were taken every week for up to 6 weeks (T0–T5). The teeth were segmented from the scans and the scans were superimposed on the palatal rugae. The rate of OTM was calculated for each tooth. Other parameters were gingival inflammation, contact-point displacement and the biological markers, matrix metalloproteinases (MMP), MMP-9 and MMP-2 in gingival crevicular fluid (GCF). Results Our study showed a high variation in the rate of OTM, varying from 0.15 to 1.24 mm/week. Teeth in the anterior segment tended to move more compared with the posterior segment. The contact point displacement and gingival inflammation varied greatly amongst the patients. The MMPs measured did not correlate with tooth movement. However, the gingival inflammation index showed a significant correlation with OTM. Future studies should include other biological markers related to bone-remodeling. Conclusion This novel and efficient 3D method is suitable for measuring OTM and showed large individual variation in rate of OTM. Clinical relevance Patients show different rates of OTM. The rate of OTM in an individual patient can provide guidance in timing of follow-up appointments.

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