Amide I Band Analysis Applied to Vibrational Micro-Spectroscopies of Gingival Crevicular Fluid Samples for Orthodontic Treatment Monitoring

Vibrational micro-spectroscopies were applied to investigate the gingival crevicular fluid (GCF) for monitoring orthodontic treatment with fixed appliances. The GCF samples were investigated using Fourier transform infrared, Raman, and surface-enhanced Raman micro-spectroscopies. The GCF spectra collected at different times of orthodontic tooth movement were used to characterize the biochemical changes occurring during the treatment. We examined the amide I band region by means of deconvolution analysis using Gaussian–Lorentzian curves for infrared spectra and Lorentzian curves for Raman spectra. This analysis allowed us to evidence the contribution of the different subcomponents of the amide I band and the changes occurring during orthodontic treatment. These changes can be ascribed to modifications in the secondary structure of protein content and could contribute to make vibrational spectroscopies a useful tool for monitoring the individual patient’s response to orthodontic force application.

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Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

Journal of Clinical Medicine ◽

10.3390/jcm10071405 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1405

Author(s):

Fabrizia d’Apuzzo ◽

Ludovica Nucci ◽

Ines Delfino ◽

Marianna Portaccio ◽

Giuseppe Minervini ◽

...

Keyword(s):

Periodontal Ligament ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

High Sensitivity ◽

Data Analysis Procedure ◽

Vibrational Spectroscopies ◽

Composition Structure ◽

The Individual ◽

Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

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Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

Applied Sciences ◽

10.3390/app11020521 ◽

2021 ◽

Vol 11 (2) ◽

pp. 521

Author(s):

Simina Chelărescu ◽

Petra Șurlin ◽

Mioara Decusară ◽

Mădălina Oprică ◽

Eugen Bud ◽

...

Keyword(s):

Young Adults ◽

Orthodontic Treatment ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Early Stage ◽

Statistical Tests ◽

Orthodontic Tooth Movement ◽

Adolescents And Young Adults ◽

Periodontal Tissues ◽

Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

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MicroRNA-34 expression in gingival crevicular fluid correlated with orthodontic tooth movement

The Angle Orthodontist ◽

10.2319/090219-574.1 ◽

2020 ◽

Vol 90 (5) ◽

pp. 702-706

Author(s):

Bin Zhang ◽

Li Yang ◽

Weilong Zheng ◽

Ting Lin

Keyword(s):

Baseline Level ◽

Orthodontic Treatment ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Level 3 ◽

Polymerase Chain ◽

Crevicular Fluid

ABSTRACT Objectives To explore the expression of miR-34a and its effect on expression of matrix metalloproteinases (MMPs) during orthodontic tooth movement (OTM). Materials and Methods Twenty patients, age 12–18 years old, who underwent orthodontic treatment were enrolled. The expression of miR-34a and MMPs (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMP-14) were detected in gingival crevicular fluid by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction at different time points. The miR-34a mimics or inhibitors were transfected into human periodontal ligament (hPDL) cells, and the MMP expression was measured by ELISA. Results The miR-34 expression in GCF on both the tension and pressure sides after orthodontic treatment were significantly downregulated, while the levels of MMPs were significantly upregulated compared with baseline level. The levels of miR-34 and MMPs returned to baseline level 3 months after orthodontic treatment. The expression of miR-34 was negatively correlated with the expression of MMP-2, MMP-9, and MMP-14. After transfection with miR-34, the MMP-2, MMP-9, and MMP-14 expression by hPDL cells were significantly downregulated compared with miR-control and miR-34 inhibitor. Conclusions Downregulated miR-34 expression was positively correlated with MMP-2, MMP-9, and MMP-14 expression. The miR-34a transfection into hPDL cells inhibited expression of MMPs. The results suggest that miR-34a is involved in expression of MMPs during OTM.

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Detection and assessment of Cathepsin K in gingival crevicular fluid during human orthodontic tooth movement

IP Indian Journal of Orthodontics and Dentofacial Research ◽

10.18231/j.ijodr.2019.031 ◽

2019 ◽

Vol 5 (4) ◽

pp. 143-146

Author(s):

Kiran H ◽

◽

Rajkumar S. Alle ◽

Dharmesh H.S ◽

Bharathi V.S ◽

...

Keyword(s):

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Orthodontic Tooth Movement ◽

Cathepsin K ◽

Crevicular Fluid

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Highly variable rate of orthodontic tooth movement measured by a novel 3D method correlates with gingival inflammation

Clinical Oral Investigations ◽

10.1007/s00784-020-03502-2 ◽

2020 ◽

Author(s):

Marc A. de Gouyon Matignon de Pontouraude ◽

Johannes W. Von den Hoff ◽

Frank Baan ◽

Robin Bruggink ◽

Marjon Bloemen ◽

...

Keyword(s):

Biological Markers ◽

Orthodontic Treatment ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Contact Point ◽

Anterior Segment ◽

Three Dimensional ◽

Orthodontic Tooth Movement ◽

Large Individual ◽

Gingival Inflammation

Abstract Objectives Individual orthodontic treatment duration is hard to predict. Individual biological factors are amongst factors influencing individual rate of orthodontically induced tooth movement (OTM). The study aim is to determine the rate of OTM by a novel 3D method and investigate parameters that may predict the rate of tooth movement. Materials and methods In this prospective cohort study, rate of OTM was determined from 90 three-dimensional intra-oral scans in 15 patients (aged 12–15) undergoing orthodontic treatment. For each patient, intra-oral scans were taken every week for up to 6 weeks (T0–T5). The teeth were segmented from the scans and the scans were superimposed on the palatal rugae. The rate of OTM was calculated for each tooth. Other parameters were gingival inflammation, contact-point displacement and the biological markers, matrix metalloproteinases (MMP), MMP-9 and MMP-2 in gingival crevicular fluid (GCF). Results Our study showed a high variation in the rate of OTM, varying from 0.15 to 1.24 mm/week. Teeth in the anterior segment tended to move more compared with the posterior segment. The contact point displacement and gingival inflammation varied greatly amongst the patients. The MMPs measured did not correlate with tooth movement. However, the gingival inflammation index showed a significant correlation with OTM. Future studies should include other biological markers related to bone-remodeling. Conclusion This novel and efficient 3D method is suitable for measuring OTM and showed large individual variation in rate of OTM. Clinical relevance Patients show different rates of OTM. The rate of OTM in an individual patient can provide guidance in timing of follow-up appointments.

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