scholarly journals PENGARUH PEMBERIAN KADMIUM TERHADAP TINGKAT KELANGSUNGAN HIDUP DAN KERUSAKAN STRUKTUR INSANG DAN HEPATOPANKREAS PADA UDANG REGANG [Macrobrachium sintangense (de Man)]

2004 ◽  
Vol 10 (1) ◽  
pp. 59-66
Author(s):  
Agoes Soegianto ◽  
Nia Adiani Primarastri ◽  
Dwi Winarni

The purpose of this research was to find out the impact of cadmium on the structure of gills, hepatopancreas and on survival of shrimp. The shrimp was divided into four groups. Group I (control) was exposed to 0 ppb of cadmium, group II exposed to10 ppb of Cd, group III exposed to 20 ppb of Cd and group IV exposed to 30 ppb of Cd. Three replications were applied in each treatment. Survival rate data were collected everyday, and every two days the water was substituted with new water. The experiment was stoped when 50 percent of shrimps dead. The rest of shrimps were prepared for histological study. All data were subjected on Kolmogorov-Smirnov (distribution test) then continued with ANOVA test and Kruscal-Wallis tests. The result of this experiment showed that structural damage on gills and hepatopancreas increase with increasing cadmium concentration in medium. The lamella of shrimp from control group did not show hyperplasia and necrosis; in second group: 24.02 percent of lamella showed hyperplasia, 2.77 percent necrosis and 28.02 percent vacuolization; third group: 70.01 percent of lamella showed hyperplasia, 20.60 percent necrosis and 48.79 percent vacuolization; fourth group: 32.60 percent of lamella demonstrated hyperplasia, 57.35 percent necrosis and 97.50 percent vacuolization. Increasing the structural damage of gills and hepatopancreas, it cause the decrease on survival rate of shrimp.

Author(s):  
Krishna Mohan Kumar

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.


2008 ◽  
Vol 24 (9) ◽  
pp. 595-601 ◽  
Author(s):  
JP Xu ◽  
YP Yin ◽  
XQ Zhou

The study was conducted to determine the effects of vitamin E on reproductive performance in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The 75 female and 40 male mice were randomly assigned into five groups. The levels of both TCDD and vitamin E given by gavage were 0 and 0 (Control group), 100 and 0 (experimental group I), 100 and 20 (experimental group II), 100 and 100 (experimental group III), and 100 ng/kg/day and 500 mg/kg/day (experimental group IV), respectively. Males and females were mixed to mate at the ratio of 1:2 after 4-week experiment. The gavage treatments were continued until the end of gestation in female mice after mating. The results showed that the litter number, survival rate, and body weight at birth of offspring in experimental group I had significantly decreased, and the females’ pregnancy rate and pup sex ratio in experimental group I had the decreasing tendencies when compared with the control group. The litter number in experimental group III, survival rate in experimental group II and III, body weight at birth in experimental group III and IV exhibited significant increase compared with experimental group I. The female pregnancy rate in both experimental group III and IV recovered to 100%, but there were no significant differences when compared with experimental group I. The pups’ sex ratio had a gradually increasing tendency with increase of vitamin E level, but there was no significant difference among experimental group I–IV. The results suggest that TCDD could induce reproductive toxicity in mice, whereas vitamin E alleviated adverse effects on reproductive performance in mice caused by TCDD.


Author(s):  
SALEH NUHU ◽  
HAUWA IDRIS AHMAD ◽  
AISHA MUHAMMAD GARBA ◽  
TASIU ABDULLAHI SULAIMAN

Objectives: The objective of this study was to find the histologic and motor activity effect of lead on prenatally and postnatally exposed Wistar rats. Methods: In this study, twelve Wistar Rats were used and grouped into four groups of two females and one male. Group I rats served as the control and allowed feed and water freely. The rats in Group II were administered 500ppm of Pb through drinking water from gestation day 8 (GD8) to parturition (GD21). While Group III rats were given 500ppm of Pb in drinking water from postnatal day 1 (PND1) to PND21. The rats in the fourth group (Group IV) were given 500ppm of Pb from GD8 to PND21. Palmer grasp reflex was conducted to assess the motor activity of the rat pups. The animals were then humanely sacrificed and the frontal cortices were isolated for routine histological processing. Results: The histological study has shown normal neurons in the control group while degenerating cells exhibiting karyolysis, pyknosis, karyorrhexis, vacuolation were seen in the lead-treated groups. Group II and Group IV showed considerate deficit in their motor activity while Group III showed mild effect. Conclusion: From this study, lead exposure of Wistar rats at both prenatal and postnatal period of development has effect on the histology of the frontal cortex as well as on their motor activity.


Author(s):  
Ashlesh M Upadhyaya ◽  
Mandava V Rao ◽  
Devendrasinh D Jhala

 Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II - melatonin alone (10 mg/kg body weight); Group III - 2,4-D alone (50 mg/kg body weight); and Group IV - 2,4-D + melatonin was given orally for 45 days.Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules.Conclusion: Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity. 


2018 ◽  
Vol 11 (1) ◽  
pp. 10
Author(s):  
Etimad Alattar ◽  
Khitam Elwasife ◽  
Eqbal Radwan ◽  
Hadeer Abu Warda ◽  
Mohammed Abujami

The main objective was to determine the effect of mobile phone radiation on general health and electrolytes among students who use mobile phones. A questionnaire was designed and applied to 103 healthy and 36 deaf female students to select cases that meeting the inclusion criteria. For assessment of salivary parameters, the participants were classified into three groups. Group I was the control group, which included 17 deaf students. Group II was healthy students who have mobile phone for less than 5 years. Group III was healthy students who have mobile phone for 5 years or more. The results showed that the participants who use mobile phone had several problems in their health including dry mouth, bad odor from mouth, drooling of saliva, as well as ear and eye pain. The participants who use mobile phone complained of headache, anxiety and forgetfulness as compared to deaf participants. The study showed that there was no significant difference between pH and flow rate of saliva in all tested groups. This study has also shown that salivary level of Na+ and K+ were significantly lower in mobile phone users when compared to non-users of mobile phone.


2021 ◽  
Vol 17 (4) ◽  
pp. 809-817
Author(s):  
Al-Qtaitat Aiman ◽  
Al-Dalaien Said ◽  
Albtoosh Amal ◽  
Fardous Karawya

Glucocorticoids is the most common cause of secondary osteoporosis. Osteoporosis is widespread, costly and causes pain, deformity and disability. Several studies report health benefits of raisins. Raisins have a combination of compounds with antibacterial, antioxidant,anticarcinogenic and anti-inflammatory properties. The present study investigated the impact of Raisins onmethylprednisolone-induced osteoporosis in rats. Thirty male albino rats were randomly divided into three main groups, ten rats each. Group I control group; Group II, osteoporotic group, where osteoporosis was induced by injection of methylprednisolone; Group III, protected group, animals were given raisinsconcomitant daily with methylprednisolone. Bone biochemical markers were assisted, hydroxyproline, urinary calcium excretion, serum calcium, alkaline phosphatase and osteocalcin. In addition to histological findings in lumbar vertebrae of the rats.Our findings showed that raisin has a positive osteoprotective effects on methylprednisolone induced bone changes histologically and over the biomarkers examined.This study suggests that dietary raisins may moderate methylprednisolone bone induced changes.


Author(s):  
Etimad Alattar ◽  
Khitam Elwasife ◽  
Eqbal Radwan ◽  
Hadeer Abu Warda ◽  
Mohammed Abujami

The main objective was to determine the effect of mobile phone radiation on general health and electrolytes among students who use mobile phones. A questionnaire was designed and applied to 103 healthy and 36 deaf female students to select cases that meeting the inclusion criteria. For assessment of salivary parameters, the participants were classified into three groups. Group I was the control group, which included 17 deaf students. Group II was healthy students who have mobile phone for less than 5 years. Group III was healthy students who have mobile phone for 5 years or more. The results showed that the participants who use mobile phone had several problems in their health including dry mouth, bad odor from mouth, drooling of saliva, as well as ear and eye pain. The participants who use mobile phone complained of headache, anxiety and forgetfulness as compared to deaf participants. The study showed that there was no significant difference between pH and flow rate of saliva in all tested groups. This study has also shown that salivary level of Na+ and K+ were significantly lower in mobile phone users when compared to non users of mobile phone.


2018 ◽  
Vol 68 (2) ◽  
pp. 231
Author(s):  
S. KAYA ◽  
M. ÖĞÜN ◽  
H. ÖZEN ◽  
M. KURU ◽  
L. ŞAHIN ◽  
...  

This study was aimed to investigate the presence of uterine infection and the relationship between the severity of uterine infection and some oxidative stress parameters in cows in early postpartum period. Sixty six cows that were in the period between the 30th and 32rd days of postpartum were included in the study. Endometrial inflammation was scored according to the condition of the uterus at rectal and ultrasonographic examinations and the character and amount of uterine discharge from endometritis I through III, the latest being the most severe. Endometrial samples were taken from the clinically healthy animals by cytobrush method, and then stained with Giemsa for presence of polymorphonuclear leukocytes (PMN). Cows with a PMN percentage less than 18% were considered to be healthy (control, n=20). All cows were sampled for blood. Nitric oxide (NO) levels in Group I (Endometritis I; n=20), Group II (Endometritis II; n=16), Group III (Endometritis III; n=10) and the control group were determined to be 23.0±0.63 nmol/mL, 32.23±0.97 nmol/mL, 36.56±0.48 nmol/mL, and 11.10±0.29 nmol/mL, respectively. The differences among the groups were found to be statistically significant. Malondialdehyde (MDA) level was highest in Group III (6.38±0.13 μmol/L), and its level significantly decreased with the reduced severity of endometritis. The comparison of the groups for total antioxidant capacity (TAC) and total oxidant capacity (TOC) demonstrated that endometritis caused a decrease in TAC levels (P<0.05), but did not affect TOC levels. Endometritis increased serum NO and MDA levels, and decreased TAC levels, but had no effect on TOC levels. In conclusion, NO and MDA, as well as TAC can be used as a biochemical marker for estimation of the severity of endometritis in cows.


Author(s):  
Ashlesh M Upadhyaya ◽  
Mandava V Rao ◽  
Devendrasinh D Jhala

 Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II - melatonin alone (10 mg/kg body weight); Group III - 2,4-D alone (50 mg/kg body weight); and Group IV - 2,4-D + melatonin was given orally for 45 days.Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules.Conclusion: Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity. 


Author(s):  
Anton Bózner ◽  
Mikuláš Gažo ◽  
Jozef Dostál

It is anticipated that Japanese quail /Coturnix coturnix japonica/ will provide animal proteins in long term space flights. Consequently this species of birds is of research interest of international space program INTERCOSMOS. In the year 1987 we reported on an experiment /2/ in which the effect of chronic acceleration of 2 G hypergravitation, the hypodynamy and the simultaneous effect of chronic acceleration and the location in the centre of the turntable of the centrifuge on the protein fractions in skeletal muscles was studied. The ultrastructure of the heart muscle was now in this experiments examined as well.Japanese quail cockerels, aged 48 days were exposed to 2 G hypergravitation /group IV/ in a 6,4 m diameter centrifuge, to hypodynamy /group III/ and their combination /group V/, respectively for 6 days / Fig.1/. The hypodynamy in group III was achieved by suspending the birds in jackets without contact the floor. The group II was located in the centre ofthe turntable of the centrifuge. The control group I. was kept under normal conditions. The quantitative ultrastructure of myocard was evaluated by the methods of Weibel/3/ - this enables to determine the number, relative size and volume of mitochondria volume of single mitochondria, defficiency of mitochondrial cristae and volume of myofibrils.


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