scholarly journals CONSORTS MONITORING IN ECOSYSTEM: PLANT – PHYTOPATHOGENS – SOIL SAPROTROPHES

Author(s):  
E.Yu. Toropova ◽  
◽  
I.G. Vorobyova ◽  
R.I. Trunov ◽  
V.V. Piskarev ◽  
...  

Monitoring studies were carried out according to generally accepted methods in the northern forest-steppe of West Siberia in 2019–2020, using the varieties collection of the Institute of Cytology and Genetics SB RAS. Studies have shown that the genotypes of spring wheat varieties had a significant impact on the functioning of the “plant-phytopathogensaprotrophs” system. The parasitic activity of phytopathogens (Bipolaris sorokiniana Sacc. Shoem., Fusarium spp. Link.) differed in the varietal groups to 43.6 %. Varieties of Asian origin (China, India, Kazakhstan, Syria) were most affected by the root infections, Australian varieties were least affected by phytopathogens, due to the chemical composition of root exudates, which suppressed the number of parasitic and saprotrophic microorganisms. Varietal secretions of the wheat plants roots regulated the number of saprotrophic rhizospheric microorganisms. The share of the influence of the “variety” factor on the number of saprotrophic soil microorganisms was 24.3 % and was reliable at a 1 % level. The most sensitive to the composition and number of root exudates of wheat plants turned out to be bacteria of various trophic groups, the most resistant were micromycetes. In comparison with the background indices of non-rhizospheric soil, the number of cellulosolytic bacteria increased up to 3 times (West European varieties), bacteria consuming organic nitrogen – up to 5.6 times (Asian varieties), consumers of organic nitrogen forms – up to 2.9 times (West European varieties), oligonitrophilic bacteria – up to 12.7 times (Siberian varieties). Varieties far from the region of the study (Australia, Africa, America) exerted a repressive effect on the trophic groups of microorganisms, reducing their number in comparison with indicators of non-rhizospheric soil.

2018 ◽  
Vol 12 (4) ◽  
pp. 61-66
Author(s):  
Владимир Шаманин ◽  
Vladimir Shamanin ◽  
Инна Потоцкая ◽  
Inna Potockaya ◽  
Олег Кузьмин ◽  
...  

In connection with climate warming and, consequently, the deterioration of phytopathological situation, associated with the emergence of aggressive races of fungal diseases, as well as the cultivation of susceptible varieties on the main wheat sowing area in Western Siberia, it is important to expand the genotypic variety of wheat varieties and to search for new sources of long-term sustainability. The results of the assessment of spring soft wheat varieties of the KASIB breeding nursery, set up jointly by the scientific institutions of Western Siberia, Kazakhstan and CIMMYT International Center for the Improvement of Corn and Wheat are presented in the conditions of the southern forest-steppe of West Siberia. The research was conducted in 2011-2016, using field and laboratory methods. The results of the assessment of susceptibility of KASIB varieties to brown and stem rust have shown, that varieties with high resistance prevail among resistant varieties in all phases of plant development, which indicates targeted selection for immunity with genes of race-specific and age resistance. The use of cluster analysis made it possible to identify significant differences in productivity and resistance to rust diseases of the varieties of KASIB program. Wheat varieties from KASIB are underlined: 14, 15 - Lutestsens 141/03-2 and Sigma (SibNIISKh), from KASIB nursery 16, 17 - Erytrospermum 85-08 (Omsk SAU), Lutestsens 6/04-4 and Lutestsens 186/04- 61 (SibNIISKh), resistant to brown and stem rust, exceeding in terms of yield standards, which are recommended to be used as a starting material for wheat selection in Western Siberia.


2019 ◽  
pp. 24-28 ◽  
Author(s):  
M. G. Evdokimov ◽  
V. S. Yusov ◽  
I. V. Pakhotina ◽  
M. N. Kiriyakova

There have been presented study results of the spring durum wheat varieties grown in the southern forest-steppe area of the Omsk region on kernel hardness. The objects of research were the varieties of ecological variety testing conducted by various Russian and Ukrainian scientific institutions. The study was conducted in the period from 2003–2015. Experimental plots of 10 m2 were placed in 4 sequences. The varieties were sown in weedfree fallow. The soil of the experimental plot was weakly leached blackearth (chernozem), medium humus (6.2%), and loamy. The sowing date is 14–15 of May, the sowing rate is 4.5 million of germinated kernels per ha. The average hardness index in 2003–2015 was 70.1%. The varieties varied from 66.0 (the variety “Svetlana”) to 74.6% (the variety “Saratovskaya zolotistaya”). The differences between maximum and minimum were 8.6%. The variation through the years was from 51% to 92%, from 21% (the varieties “Angel”, “Omsky korund”) to 34% (the variety “Saratovskaya zolotistaya”). The calculated coefficient of variation showed that the variability degree ranged from little to medium. Coefficients of variation varied from 9.3% (the variety “Angel”) to 14.3% (the variety “Bezenchukskaya 182”). According to S. A. Eberhart, W. A. Russel the stability index indicates a lower variability of the varieties “Angel”, “Omsky korund”, “Altayskaya niva”, “Nik”, “Kharkovskaya 23”, “Tavolga”. The value of the regression coefficient (bi) for kernel hardness ranged from 0.77 to 1.23. The varieties “Voronezhskaya 9”, “Elizavetinskaya”, “Altayskaya niva”, “Omskaya stepnaya” and “Bezenchukskaya stepnaya” turned to be most responsive to the conditions (according to the Eberhart – Russell test). The varieties “Angel”, “Aleyskaya”, “Zarnitsa Altaya” had a weak reaction to the environmental conditions. There has been identified phenotypic correlation between the trait and productivity, 1000-kernel weight, nature weight, gluten quality, pasta color. The correlation between these traits is positive, on average r = 0.30–0.440.


2019 ◽  
Vol 49 (3) ◽  
pp. 41-46
Author(s):  
R. I. Polyudina

The results of breeding work on creation of the new cultivar of red clover are presented. The hybrid population (15-10-D (2n)) was created by the method of crossbreeding and selection in the conditions of artifi cial climate. Selection of winterhardy high-yielding forms of this hybrid population was conducted in the forest-steppe zone of West Siberia. The population 15-10-D (2n) was tested in three cycles of competitive variety trial (crops of 2006, 2008 and 2010) and was submitted to the State variety testing under the name Prima. According to the results of the competitive variety trial during 2007–2013, the new early-ripening diploid cultivar Prima showed high winter hardiness of 97.0-99.0%. The duration of the growing season is 115 days (on the level of standard early-ripening tetraploid cultivar Meteor). The yield of green mass in two cuts is 19.6-58.7 t/ha, the yield of dry matter is 4.2-13.5 t/ha, the yield of seeds is 276-392 kg/ ha. The average yield of green mass in two cuts of the new cultivar is 38.8 t/ha, the average yield of dry matter is 8.7 t/ha, the average yield of seeds is 317 kg/ha, which exceeds standard cultivar Meteor by 52%. The seed content of Prima cultivar is 52%, Meteor – 38%. The crude protein content in dry matter is 16.1%, the fi ber content is 26.0%. Prima cultivar has been included in the State Register of Breeding Achievements of the Russian Federation to be grown in the West Siberian region since 2019. The copyright certifi cate and patent for the breeding achievement were received.


2019 ◽  
pp. 3-27 ◽  
Author(s):  
L. M. Kipriyanova ◽  
M. A. Kleshchev

Information on the aquatic and semiaquatic vegetation of the major watercourses of West Siberia is relatively scarce, while this of small rivers is practically absent. There are 430 rivers whose length exceeds 10 km within the Novosibirsk Region. The aim of our work was to study the phytocenotic diversity of their aquatic and semiaquatic vegetation. 130 geobotanical relevés were performed by the authors in July–August 2003–2005. The object was aquatic communities formed by true aquatic (submergent and floating-leaved) plants and semiaquatic ones with emergent plants and these of water′s edge. In total 10 watercourses were studied (Table 1): 2–3 in each of five geomorphologic regions within the forest-steppe zone (Table 2). The upper, middle and lower courses (Fig. 1, Table 1) with the length of 2–2.5 km were studied in each case to ensure the 4–5 repetition of main elements (stream pools and ridges) that would give a reliable information on their flora and vegetation. The sample plots were selected above the settlements, whenever possible in sites with no to intensive anthropogenic impact. The data were collected according to J. Braun-Blanquet (1964) approach. The relevés were done on the sample plot of 100 m2 placed in the most homogeneous part of the community or, in case of its smaller area, within the natural boundaries. The following scale was used for abundance estimation: r — the species is extremely rare; + — rare, small cover; 1 — the number of individuals is large, the cover is small or individuals are sparse, but the cover is large; 2 —cover of 5–25 %; 3 — 26–50 %; 4 — 51–75 %; 5 — more than 75 %. The date on water depth, transparency (on a white Secchi disk with a diameter of 30 cm), temperature and the flow rate were obtained. The soil mechanical composition and color, the degree and nature of anthropogenic impact on vegetation and river banks were quantified. Water samples for general chemical analysis were taken in the middle course of each river. Computer programs TURBOVEG and MEGATAB (Hennekens, 1996) were used for database. The syntaxonomic affiliation of phytocenoses was determined using modern literature (Bobrov, Chemeris, 2006; Vegetace..., 2011; Chepinoga, 2015; Landucci et al. 2015; Mucina et al., 2016, etc.). 36 associations and 3 communities belonging to 12 alliances, 9 orders, and 5 classes have been identified (Tables 3–14, Fig. 2–9). Such great syntaxonomic diversity is determined by the significant ecotopic variety, the variability of substrates, the wide range of water flow rates and the different water trophicity. For comparison, 26 associations, 13 variants, 2 communities were identified in the study of 50 rivers of Lithuania (Sinkyavichene, 1992); altogether 84 associations are known for the Upper Volga region as a whole (Bob­rov, Chemeris, 2006), while 45 ones were recorded previously in 130 watercourses of this region (Bobrov, 1999). Information on small river macroalgae cenoses in the study area is partially reflected in the paper published earlier (Bobrov et al., 2005).


2021 ◽  
pp. 1-8
Author(s):  
Deep Shikha ◽  
Chandani Latwal ◽  
Elangbam Premabati Devi ◽  
Anupama Singh ◽  
Pawan K. Singh ◽  
...  

Abstract Genetic resources are of paramount importance for developing improved crop varieties, particularly for biotic and abiotic stress tolerance. Spot blotch (SB) is a destructive foliar disease of wheat prevalent in warm and humid regions of the world, especially in the eastern parts of South Asia. For the management of this disease, the most effective measure is the development of resistant cultivars. Thus, the present investigation was carried out to confirm SB resistance in 200 germplasm accessions based on phenotypic observations and molecular characterization. These elite breeding lines obtained from the International Centre for Maize and Wheat Improvement, Mexico, are developed deploying multiple parentages. These lines were screened for SB resistance in the field under artificially created epiphytotic conditions during 2014–15 and 2015–16 along with two susceptible checks (CIANO T79 and Sonalika) and two resistant checks (Chirya 3 and Francolin). Eighty-two out of 200 germplasm accessions were found resistant to SB and resistance in these lines was confirmed with a specific SSR marker Xgwm148. Three accessions, VORONA/CNO79, KAUZ*3//DOVE/BUC and JUP/BJY//URES/3/HD2206/HORK//BUC/BUL were observed possessing better resistance than the well-known SB-resistant genotype Chirya3. These newly identified resistant lines could be used by wheat breeders for developing SB-resistant wheat varieties.


2021 ◽  
Vol 2 (44) ◽  
pp. 22-22
Author(s):  
Alexander Saakian ◽  
◽  

The taxonomic composition and incidence of phytopathogenic fungi on the roots of soft spring wheat Triticum aestivum L. of nine varieties of Siberian origin (Altayskaya 70, Altayskaya 75, Krasnoyarskaya 12, Novosibirskaya 15, Novosibirskaya 16, Novosibirskaya 29, Novosibirskaya 31, Novosibirskaya 41 and Svirel) cultivated using wheat and fallow as a predecessor, was studied in the area of Kansk-Krasnoyarsk forest-steppe. Average incidence of fungal root infection was 24%. In plants grown using wheat as a predecessor, the incidence was statistically significantly (p <0.05) higher than in plants grown using fallow as a predecessor (27.3 versus 20.6%). Statistically significant (p <0.05) differences in the prevalence of root infection were revealed between cultivars. The maximal prevalence (33.3 and 32.3%, respectively) on average for the wheat predecessor and fallow was found for the varieties Svirel and Altayskaya 75, the minimal (16.7%) for the varieties Novosibirskaya 16 and Altayskaya 70. The complex of phytopathogenic fungi on the roots is represented by Fusarium spp., Bipolaris sorokiniana and Alternaria spp. (31.4, 44.9 and 23.7% of the pathogenic complex on average for varieties and variants, respectively). The composition of pathogens statistically significantly (p <0.01) depends on the predecessor. In the plants cultivated using wheat as a predecessor, the proportion of Alternaria spp. was higher whereas proportions of Fusarium spp. and Bipolaris sorokiniana were lower. No differences in prevalence and taxonomic composition of root infection between varieties originated from Novosibirsk territory, Krasnoyarsk territory and Altay territory were found. Keywords: SPRING WHEAT, ROOT ROT, KRASNOYARSK TERRITORY, FUSARIUM SPP., BIPOLARIS SOROKINIANA, ALTERNARIA SPP


Author(s):  
L. N. Korobova ◽  
T. A. Kizimova ◽  
A. A. Pobelenskaya ◽  
T. G. Lomova

The authors studied the effect of the bacterial-humic preparation AFG-b containing the spore-forming bacteria Bacillus subtilis and Bacillus amyloliquefaciens in a production experiment in the northern forest-steppe of the Novosibirsk Priob’ye region. The preparation was used on midearly spring wheat, which forms grain at the level of valuable wheat. The drug was used as an antistressant together with herbicides against dicotyledonous and monocotyledonous plants. The tank mixture used was metsulfuron-methyl, fenoxaprop-P-ethyl with the antidote cloquintoset-mexyl and 2-ethyl hexyl ester of 2,4-D with florasulam. The bacterial-humic preparation AFG-b is known to level out herbicide stress in wheat and improve plant health. It improves the condition of leaf cell membranes by preventing the escape of electrolytes from the cells. On the roots of plants one month after applying the antistressant, the authors observed a 1.5-2 times reduction in the development and prevalence of root rot of fusarium-helminthosporiosis etiology. The authors also marked the most pronounced phytosanitary effect of the preparation’s bacteria, which are antagonists of phytopatho- gens for plants’ primary roots and epicotyl. An increase in plant productivity manifests the anti-stress and growth-stimulating effect of AFG-b. During the growing season of 2020, the bio preparation combined with herbicides provided a reliable increase of 40.2% of the grain of spring wheat and improved its quality by enhancing the protein and gluten content. Under 2019 conditions, AFG-b increased grain yield relative to herbicides by about 8% and did not affect grain quality. Application of AFG-b as an anti-stressant is not accompanied by improvement of seed quality of the new crop. It does not improve its phytosanitary status in infestation by phytopathogens Bipolaris sorokiniana, Fusarium sp., Alternaria, Stagonospora nodorum, Penicillium and Aspergillus relative to herbicides alone.


Author(s):  
Hana Kaňová ◽  
Joffrey Carre ◽  
Valerie Vranová ◽  
Klement Rejšek ◽  
Pavel Formánek

This study was conducted to determine the composition of sugars and organic acids in root exudates of Miscanthus × Giganteus and to find out if microorganisms of the rhizospheric soil are limited by mi­ne­ral nutrients. The following sugars and organic acids were determined in root exudates of this plant: glucose, saccharose, and acids such as succinic, propionic, citric, tartaric, malic, oxalic, ascorbic, acetic and fumaric. Respiration of soil from rhizosphere of Miscanthus × Giganteus was found to be limited by N, K and Ca. Respiration rate after application of mineral compounds increased in following orther: nitrate > calcium > potassium > ammonium, giving approx. 165, 99, 52 and 31 % increase compared to control. Further research is necessary to determine the role of plant nutrients from the point of their limitations for rhizosphere microorganisms, to broader very rare knowledges in this topic, especially for polluted soils to stimulate efficiency of phytoremediations.


2019 ◽  
Vol 180 (1) ◽  
pp. 38-43 ◽  
Author(s):  
P. N. Nikolaev ◽  
O. A. Yusova ◽  
N. I. Aniskov ◽  
I. V. Safonova

Background. An extremely important task today is to develop new hulless barley cultivars, capable of yielding large and high-quality grain harvests, and introduce them into agricultural production. Objective. The purpose was to study three hulless barley cultivars ‘Omsky golozerny 2’, ‘Omsky golozerny 4’ and ‘Maysky’, developed at Omsk Agrarian Scientific Center, in order to describe their agrobiological characteristics.Materials and methods.The experimental part of the work was carried out in 2015–2017 on the experimental fields of Omsk ASC in the southern forest steppe (third crop rotation after the wheat predecessor; fourth crop after fallow). There were 4 replications on the plot of 10 m2. The seeding rate was 4 million viable seeds per 1 ha. Agricultural practice used in the experiments was conventional for West Siberia. Parameters of stability and plasticity were calculated according to Eberhart and Russell, Wricke; adaptability, according to Zhivotkov; homeostasis, according to Khangildin; stress tolerance and compensatory ability, according to Rossielle and Hemblin.Results. Many years of breeding work at Omsk ASC resulted in the development of three hulless barley cultivars: ‘Omsky golozerny 2’ (listed in the State Register of the Russian Federation for regions 9, 10 and 11), ‘Omsky golozerny 4’ (submitted for the State Crop Variety Trials in 2017), and ‘Maysky’ (not included in the State Register). The new hulless barley cultivar ‘Omsky golozerny 4’, considering its higher productivity (+1.36 g to the reference in 1000 grain weight, and +0.73 t/ha to cv. ‘Maysky’), in the yield of nutrients per area unit equaled the reference ‘Omsky golozerny 2’, but exceeded ‘Maysky’ (+47.2 kg/ha of protein, +390 kg/ha of starch, and +42.4 kg/ha of crude fat). ‘Omsky golozerny 4’ was also characterized by stability (stability = 4.8), increased compensatory ability (3.29) and adaptability (103%). There was an increase in productivity with improved growing conditions (ecological plasticity = 1.25). ‘Maysky’ had higher stress tolerance (–1.35), while the reference ‘Omsky golozerny 2’ showed increased homeostasis (0.118). Thus, ‘Omsky golozerny 4’ exceeded the previous two cultivars in adaptability and stability.


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