Comparison of Different Analytical Strategies for Classifying Invasive Wetland Vegetation in Imagery from Unpiloted Aerial Systems (UAS)

Detecting newly established invasive plants is key to prevent further spread. Traditional field surveys are challenging and often insufficient to identify the presence and extent of invasions. This is particularly true for wetland ecosystems because of difficult access, and because floating and submergent plants may go undetected in the understory of emergent plants. Unpiloted aerial systems (UAS) have the potential to revolutionize how we monitor invasive vegetation in wetlands, but key components of the data collection and analysis workflow have not been defined. In this study, we conducted a rigorous comparison of different methodologies for mapping invasive Emergent (Typha × glauca (cattail)), Floating (Hydrocharis morsus-ranae (European frogbit)), and Submergent species (Chara spp. and Elodea canadensis) using the machine learning classifier, random forest, in a Great Lakes wetland. We compared accuracies using (a) different spatial resolutions (11 cm pixels vs. 3 cm pixels), (b) two classification approaches (pixel- vs. object-based), and (c) including structural measurements (e.g., surface/canopy height models and rugosity as textural metrics). Surprisingly, the coarser resolution (11 cm) data yielded the highest overall accuracy (OA) of 81.4%, 2.5% higher than the best performing model of the finer (3 cm) resolution data. Similarly, the Mean Area Under the Receiving Operations Characteristics Curve (AUROC) and F1 Score from the 11 cm data yielded 15.2%, and 6.5% higher scores, respectively, than those in the 3 cm data. At each spatial resolution, the top performing models were from pixel-based approaches and included surface model data over those with canopy height or multispectral data alone. Overall, high-resolution maps generated from UAS classifications will enable early detection and control of invasive plants. Our workflow is likely applicable to other wetland ecosystems threatened by invasive plants throughout the globe.

Comparison of Generic and Proprietary Aquatic Herbicides for Control of Invasive Vegetation : Part 2. Emergent Plants

Aquatic herbicides are one of the most effective and widespread ways to manage nuisance vegetation in the US After the active ingredient is selected, often there are numerous proprietary and generic branded products to select from. To date, limited efforts have been made to compare the efficacy of brand name and generic herbicides head to head; therefore, at tot al of 20 mesocosm trials were conducted to evaluate various 2,4 -D, glyphosate, imazapyr, and triclopyr products against alligatorweed (Alternanthera philoxeroides (Mart.) Griseb.), southern cattail (hereafter referred to as cattail, Typha domingensis Pers.), and creeping water primrose (hereafter referred as primrose, Ludwigia peploides (Kunth) P.H. Raven). All active ingredients were applied to foliage at broadcast rates commonly used in applications to public waters. Proprietary and generic 2,4 -D, glyphosate, imazapyr, and triclopyr were efficacious and provided 39 to 99% control of alligatorweed, cattail and primrose in 19 of the 20 trials. There were no significant differences i n product performance except glyphosate vs. alligatorweed (trial 1, Rodeo vs. Roundup Custom) and glyphosate vs. cattail (trial 1, Rodeo vs. Glyphosate 5.4). These results demonstrate under small -scale conditions, the majority of the generic and proprietary herbicides provided similar control of emergent vegetation, regardless of active ingredient

Effects of Water Level Fluctuations on the Growth Characteristics and Community Succession of Submerged Macrophytes: A Case Study of Yilong Lake, China

Water level fluctuation (WLF) has a significant effect on aquatic macrophytes, but few experimental studies have examined the effect of WLF on submerged community succession, especially from a large-scale perspective. In this study, field monitoring of WLF and submerged macrophytes was conducted in Yilong Lake (SE China) over two years, and the impacts of WLF on the growth characteristics and the community structure of submerged macrophytes were determined. The results show that the biomass of submerged macrophytes decreased significantly after the water level increased and submerged macrophytes could cope with the adverse environment by adjusting their growth posture, for example, increasing plant length and reducing branch number. However, different submerged plants have different regulatory abilities, which leads to a change in the community structure. Myriophyllum spicatum, Stuckenia pectinata, and Najas marina had better adaptation abilities to WLF than Najas minor and Utricularia vulgaris. Changes in water depth, dissolved oxygen, and transparency significantly contribute to the effect of WLF on submerged plant communities. Therefore, when determining the range of WLF, the above three critical factors and submerged plant species should be considered. WLF changed the spatial distribution of the aquatic plant community. When water levels rose, the density of the submerged macrophyte community in the original growth region reduced as the emergent plants migrated to shallower water, and the seed bank germination was aided by transparent water produced among emergent plants. This can be used as a pioneering measure to restore submerged plants in eutrophic lakes with low transparency. In addition, a suitable water depth created by WLF was conducive to activating the seed bank and improving the diversity of aquatic plants. Finally, a distribution map of aquatic plants in Yilong Lake is drawn.

The Roles of Competition and Facilitation in Producing Zonation Along an Experimental Flooding Gradient: A Tale of Two Tails with Ten Freshwater Marsh Plants

Plant zonation is conspicuous in wetlands. The cause is frequently assumed to be the direct physiological effects of physical factors (termed ‘stress’), however many experiments show that competition and facilitation also cause zonation patterns. We conducted a field experiment with freshwater marsh emergent plants to test the causes of zonation along a single stress gradient: flooding duration. We constructed an experimental wetland with ten flooding levels to ensure that the environmental conditions represented the full range of potential flooding levels, from never flooded to continually flooded. We planted ten common marsh plants with varied ecology along the flooding duration gradient. We grew them alone and in mixture for three years and measured changes in the minimum and maximum limits, the mode and the range of distribution, and interaction importance. The mode of distribution did not shift, whether species were grown alone or with neighbours. We found strong effects of competition under low flooding stress. We found no effects from facilitation under high flooding stress. Flooding duration alone controlled the lower limits of plants. The effects of competition were intense enough to eliminate half of the species within three growing seasons. Our experiment showed that competition and physical stresses, but not facilitation, controls the zonation of emergent macrophytes along a flooding duration gradient, at least in freshwater wetlands. Models guiding wetland restoration need to include competition as well as flood duration as causal factors, but not facilitation.

Heterogenous Impact of Water Warming on Exotic and Native Submerged and Emergent Plants in Outdoor Mesocosms

Some aquatic plants present high biomass production with serious consequences on ecosystem functioning. Such mass development can be favored by environmental factors. Temperature increases are expected to modify individual species responses that could shape future communities. We explored the impact of rising water temperature on the growth, phenology, and metabolism of six macrophytes belonging to two biogeographic origins (exotic, native) and two growth forms (submerged, emergent). From June to October, they were exposed to ambient temperatures and a 3 °C warming in outdoor mesocosms. Percent cover and canopy height were favored by warmer water for the exotic emergent Ludwigia hexapetala. Warming did not modify total final biomass for any of the species but led to a decrease in total soluble sugars for all, possibly indicating changes in carbon allocation. Three emergent species presented lower flavonol and anthocyanin contents under increased temperatures, suggesting lower investment in defense mechanisms and mitigation of the stress generated by autumn temperatures. Finally, the 3 °C warming extended and shortened flowering period for L. hexapetala and Myosotis scorpioides, respectively. The changes generated by increased temperature in outdoor conditions were heterogenous and varied depending on species but not on species biogeographic origin or growth form. Results suggest that climate warming could favor the invasiveness of L. hexapetala and impact the structure and composition of aquatic plants communities.

Wetland Restoration with Hydrophytes: A Review

Restoration cases with hydrophytes (those which develop all their vital functions inside the water or very close to the water surface, e.g., flowering) are less abundant compared to those using emergent plants. Here, I synthesize the latest knowledge in wetland restoration based on revegetation with hydrophytes and stress common challenges and potential solutions. The review mainly focusses on natural wetlands but also includes information about naturalized constructed wetlands, which nowadays are being used not only to improve water quality but also to increase biodiversity. Available publications, peer-reviewed and any public domain, from the last 20 years, were reviewed. Several countries developed pilot case-studies and field-scale projects with more or less success, the large-scale ones being less frequent. Using floating species is less generalized than submerged species. Sediment transfer is more adequate for temporary wetlands. Hydrophyte revegetation as a restoration tool could be improved by selecting suitable wetlands, increasing focus on species biology and ecology, choosing the suitable propagation and revegetation techniques (seeding, planting). The clear negative factors which prevent the revegetation success (herbivory, microalgae, filamentous green algae, water and sediment composition) have to be considered. Policy-making and wetland restoration practices must more effectively integrate the information already known, particularly under future climatic scenarios.

Interannual and Seasonal Variations of Hydrological Connectivity in a Large Shallow Wetland of North China Estimated from Landsat 8 Images

Hydrological connectivity is an important characteristic of wetlands that maintains the stability and functions of an ecosystem. This study investigates the temporal variations of hydrological connectivity and their driving mechanism in Baiyangdian Lake, a large shallow wetland in North China, using a time series of open water surface area data derived from 36 Landsat 8 multispectral images from 2013–2019 and in situ measured water level data. Water area classification was implemented using the Google Earth Engine. Six commonly used indexes for extracting water surface data from satellite images were compared and the best performing index was selected for the water classification. A composite hydrological connectivity index computed from open water area data derived from Landsat 8 images was developed based on several landscape pattern indices and applied to Baiyangdian Lake. The results show that, reflectance in the near-infrared band is the most accurate index for water classification with >98% overall accuracy because of its sensitivity to different land cover types. The slopes of the best-fit linear relationships between the computed hydrological connectivity and observed water level show high variability between years. In most years, hydrological connectivity generally increases when water levels increase, with an average R2 of 0.88. The spatial distribution of emergent plants also varies year to year owing to interannual variations of the climate and hydrological regime. This presents a possible explanation for the variations in the annual relationship between hydrological connectivity and water level. For a given water level, the hydrological connectivity is generally higher in spring than summer and autumn. This can be explained by the fact that the drag force exerted by emergent plants, which reduces water flow, is smaller than that for summer and autumn owing to seasonal variations in the phenological characteristics of emergent plants. Our study reveals that both interannual and seasonal variations in the hydrological connectivity of Baiyangdian Lake are related to the growth of emergent plants, which occupy a large portion of the lake area. Proper vegetation management may therefore improve hydrological connectivity in this wetland.

Evaluating the Effects of Acetic Acid and d-Limonene on Four Aquatic Plants

The foundation of most aquatic weed management programs in Florida is synthetic herbicides because many of these U.S. Environmental Protection Agency (USEPA)-registered products are effective, selective, and inexpensive compared with other strategies such as mechanical harvesting. However, stakeholders have expressed concern regarding their use and managers are interested in exploring alternative methods for aquatic weed control. To that end, we evaluated the efficacy, selectivity, and costs of the “natural” products acetic acid and d-limonene (alone and in combination with each other and citric acid) on the invasive floating plants waterhyacinth (Eichhornia crassipes) and waterlettuce (Pistia stratiotes), and the native emergent plants broadleaf sagittaria (Sagittaria latifolia) and pickerelweed (Pontederia cordata). These products, plus an industry-standard synthetic herbicide (diquat dibromide), were applied once as foliar treatments to healthy plants, which were grown out for 8 weeks after treatment to allow development of phytotoxicity symptoms. A 0.22% concentration of diquat dibromide eliminated all vegetation, but neither “natural” product alone provided acceptable (>80%) control of floating weeds, even when applied at the maximum concentrations under evaluation (20% acetic acid, 30% d-limonene). Citric acid (5% or 10%) had no effect on the activity of acetic acid or d-limonene, but some combinations of acetic acid and d-limonene controlled floating weeds effectively without causing unacceptable damage to native plants. However, these treatments are much more expensive than the synthetic standard and managers would realize a 22- to 26-fold increase in product cost alone without factoring in other expenses such as additional labor and application time. Combinations of acetic acid and d-limonene may have utility in some areas where the use of synthetic herbicides is discouraged, but broad-scale deployment of this strategy would likely be prohibitively expensive.

The Use of Constructed Wetland for Mitigating Nitrogen and Phosphorus from Agricultural Runoff: A Review

The loss of nitrogen and phosphate fertilizers in agricultural runoff is a global environmental problem, attracting worldwide attention. In the last decades, the constructed wetland has been increasingly used for mitigating the loss of nitrogen and phosphate from agricultural runoff, while the substrate, plants, and wetland structure design remain far from clearly understood. In this paper, the optimum substrates and plant species were identified by reviewing their treatment capacity from the related studies. Specifically, the top three suitable substrates are gravel, zeolite, and slag. In terms of the plant species, emergent plants are the most widely used in the constructed wetlands. Eleocharis dulcis, Typha orientalis, and Scirpus validus are the top three optimum emergent plant species. Submerged plants (Hydrilla verticillata, Ceratophyllum demersum, and Vallisneria natans), free-floating plants (Eichhornia crassipes and Lemna minor), and floating-leaved plants (Nymphaea tetragona and Trapa bispinosa) are also promoted. Moreover, the site selection methods for constructed wetland were put forward. Because the existing research results have not reached an agreement on the controversial issue, more studies are still needed to draw a clear conclusion of effective structure design of constructed wetlands. This review has provided some recommendations for substrate, plant species, and site selections for the constructed wetlands to reduce nutrients from agricultural runoff.

Uptake Kinetics of \({\text{NH}_{4}^{+} }\), \({\text{NO}_{3}^{-} }\) and \({\text{H}_{2}\text{PO}_{4}^{-} }\) by Typha orientalis, Acorus calamus L., Lythrum salicaria L., Sagittaria trifolia L. and Alisma plantago-aquatica Linn

The emergent plants may differ in their capacity to assimilate nutrients from eutrophic water bodies, so the utilization of suitable emergent plants is the key part for successful restoration of shallow eutrophic lakes and rivers. This research applied the depletion method to study the kinetics of uptake of nutrient (H2PO4−, NH4+, NO3−) in different nutrient stresses by the five emergent aquatic plants (Acorus calamus L., Typha orientalis, Lythrum salicaria L., Sagittaria trifolia L., Alisma plantago-aquatica Linn) in the riverine zones of Dashi River (39°30′–39°40′ N, 115°59′–116°5′ E), a shallow eutrophic river located in Fangshan District, Beijing. The results showed that at the three phosphorus levels, A. calamus and A. plantago-aquatica had the highest maximum uptake rate values for NH4+ under low to moderate phosphorus conditions, and high phosphorus, respectively. T. orientalis had the highest maximum uptake rate values for NO3− at all phosphorus concentrations, while the Michaelis-Menten constant values of L. salicaria and A. plantago-aquatica were smaller. At the three nitrogen levels, the maximum uptake rate values for H2PO4− were the greatest for A. plantago-aquatica at the low to moderate nitrogen levels and L. salicaria at high levels. Meanwhile, T. orientalis and L. salicaria had the smallest Michaelis-Menten constant values. In this study, nitrogen microbial transformations, such as nitrification, denitrification and their coupling were not measured and their role in measuring kinetics was not assessed. Thus, achieved results shall be considered as a synthesis of several processes mediated by plants, a theoretical guidance to the selection of plant species for phytoremediation of polluted water bodies with different nutrient stresses for quality improvement around the diverse rivers in Haihe River basin.