scholarly journals PENGIMBASAN KETAHANAN BIBIT PISANG AMBON KUNING TERHADAP PENYAKIT LAYU FUSARIUM DENGAN BEBERAPA JAMUR ANTAGONIS

2009 ◽  
Vol 9 (2) ◽  
pp. 130-140
Author(s):  
Loekas Soesanto ◽  
Ruth Feti Rahayuniati
Keyword(s):  
Population Density ◽  
Incubation Period ◽  
Disease Severity ◽  
Induced Resistance ◽  
Fusarium Wilt ◽  
Block Design ◽  
Antagonistic Fungi ◽  
Trichoderma Koningii ◽  
Randomized Block Design ◽  
Induce Resistance

Induced resistance of Ambon Kuning cultivar banana seedling to fusarium wilt with antagonistic fungi.  A research aiming at knowing the effect of antagonistic fungi supernatant on banana induced resistance, Fusarium wilt development, and banana growth was carried out from July up to December 2008. Randomized Block Design was used with four replicates. Treatments tested were control, with supernatant of Gliocladium virens, Trichoderma harzianum isolated from banana, ginger, and ginseng, Trichoderma koningii, and Fusarium equiseti, applied by injection to banana seedling corm and soaked for five minutes. Variables observed were incubation period, disease severity, Foc population density, germination inhibition, growth component, phenolic compound content, and supporting component. Result of the research indicated that the supernatant of G. virens, T. harzianum, and T. koningii could significantly induce resistance of the seedling showed by increasing the phenolic content such as glycoside, saponin, and tannin. The supernatant of all antagonistic fungi could effectively control the disease showed by lengthening incubation period as 48.71%, decreasing the disease severity as 53.57%, decreasing infection rate as 61.48%, increasing the antagonistic effectivity as 51.26%, decreasing the late population density as 45.35%, and decreasing the inoculum inside the plant as 60-100%. The extract could improve the seedling growth.

scholarly journals PENEKANAN HAYATI PENYAKIT MOLER PADA BAWANG MERAH DENGAN TRICHODERMA HARZIANUM, TRICHODERMA KONINGII, DAN PSEUDOMONAS FLUORESCENS P60

2007 ◽  
Vol 7 (1) ◽  
pp. 53-61
Author(s):  
Suprapto Edy Santoso ◽  
Loekas Soesanto ◽  
Totok Agung Dwi Haryanto
Keyword(s):  
Population Density ◽  
Pseudomonas Fluorescens ◽  
Incubation Period ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Research Result ◽  
Pathogen Population ◽  
Trichoderma Koningii ◽  
Spraying Method ◽  
Plot Design

Biological Suppression of Moler Disease on Shallot  by Trichoderma harzianum, Trichoderma koningii, and Pseudomonas fluorescens P60. Research aiming for (1) knowing efectivity of biological suppression with T. harzianum, T. koningii, and P. fluorescens P60 and (2) studying growth and production of shallot caused by the suppression carried out at the shallot farm. Split-Split Plot Design arranged in Randomized Completely Block Design was used with three replicates. The research result showed that P. fluorescens P60 was the most effective antagonistic agent to suppress the disease either alone or in combination while T. harzianumi and T. koningii did not suppress effectively. Pseudomonas fluorescens P60 could suppress the disease up to 41.96%. The best method of P. fluorescens P60 application was spraying method for 10 mL with 107 cfu/mL population density, which was able to dicrease incubation period, disease intensity, and final pathogen population up to 62.46, 18.19, and 80.67%, respectively. Growth and production of the crop tended to increase resulted from biological suppression by P. fluorescens P60, but not by T. harzianum nor T. koningii.

scholarly journals Pemanfaatan Tumbuhan Penghasil Minyak Atsiri untuk Pengendalian Fusarium oxysporum f. sp. cubense Penyebab Penyakit Layu Fusarium pada Tanaman Pisang

2011 ◽  
Vol 21 (4) ◽  
pp. 331
Author(s):  
Riska ◽  
Jumjunidang ◽  
Catur Hermanto
Keyword(s):  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Incubation Period ◽  
Fusarium Wilt ◽  
Block Design ◽  
Control Treatment ◽  
Initial Number ◽  
Combination Treatments ◽  
Lemon Grass ◽  
Randomized Block Design

Penyakit layu yang disebabkan oleh cendawan Fusarium  oxysporum f. sp. cubense (Foc) merupakan salah satu penyakit penting pada tanaman pisang. Teknik pengendalian yang efektif dan berwawasan lingkungan perlu terus diupayakan, di antaranya melalui penggunaan pestisida nabati. Penelitian ini bertujuan mengetahui pengaruh pemberian daun beberapa tumbuhan penghasil minyak atsiri terhadap jumlah propagul awal Foc dalam tanah dan pengendalian penyakit layu Fusarium pisang pada skala rumah kasa. Penelitian dilakukan di Laboratorium Penyakit dan Rumah Kasa Balai Penelitian Tanaman Buah Tropika Solok mulai bulan Februari sampai dengan Juni 2009. Rancangan yang digunakan dalam percobaan ialah acak kelompok dengan lima perlakuan dan empat ulangan, masing-masing ulangan terdiri atas lima tanaman. Perlakuan tersebut adalah empat jenis daun tumbuhan penghasil minyak atsiri yaitu : (A) daun nilam, (B) serai, (C) daun kayu manis, (D) daun cengkeh, dan (E) tanpa perlakuan (kontrol). Tanaman uji adalah bibit pisang Ambon Hijau hasil perbanyakan kultur jaringan umur 2  bulan setelah aklimatisasi. Hasil penelitian menunjukkan bahwa pemberian daun tumbuhan penghasil minyak atsiri mampu menekan jumlah propagul awal Foc di dalam media tanam. Persentase penurunan propagul Foc awal dalam media yang berumur 5 minggu setelah pemberian tumbuhan penghasil minyak atsiri berkisar antara 50,1-70,6%. Semua perlakuan, kecuali daun nilam, juga mampu memperlambat munculnya gejala atau masa inkubasi penyakit. Masa inkubasi penyakit paling lama terjadi pada perlakuan pemberian daun cengkeh, diikuti dengan perlakuan pemberian daun kayu manis dan daun serai dengan perpanjangan masa inkubasi masing-masing sampai 22 dan 15 hari dibandingkan dengan kontrol. Pemberian daun tumbuhan mengandung minyak atsiri belum berakibat pada penurunan persentase dan intensitas serangan penyakit, sehingga perlakuan pemberian tumbuhan penghasil minyak atsiri perlu dikombinasikan dengan metode pengendalian lain agar lebih efektif dalam menekan penyakit layu Fusarium.<br /><br /><br /><br />Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is the most important disease on banana. Effective and environmental friendly techniques in controlling the disease need to be effort continually, among of them are with application of biopesticide to suppres Foc. The objectives of the research were to know the effect of some plant producing essential oils on initial number of propagule of Foc in soil and disease development of Fusarium wilt of banana. The research was conducted at Indonesian Tropical Fruits Research Institute Solok from February to June 2009. A randomized block design with five treatments and four replications was used, whereas each treatment consisted of five plants. Four types of plant producing essential oils as treatments, namely (A) crude of patchouly leaves, (B) crude of lemon grass, (C) crude of cassia leaves, (D) crude of clove leaves, and (E) water as control treatment were used. Ambon Hijau cultivar derived from tissue culture propagation of 2 months after acclimatization was used as experiemental material. The result showed that application of leaves of plant producing essential oils decreased initial number of Foc propagules in the banana cultivation media. Percentage of reducing the number of initial propagule of Foc in medium after infestation of plant producing essential oils ranged between 50.1-70.6%. All application of plant producing essential oils, except crude of patchouly leaves, was effective to reduce the incidence of wilting or incubation period of the disease.  The longest disease incubation period was determined on treatment with clove leaves, followed by cassia and lemon grass leaf with extending incubation period up to 22 and 15 days respectively compared to control. Application of the plant producing essential oils was not successfully applied in suppressing the percentage of wilt and disease intensity on banana under screenhouse condition. Therefore combination treatments with other techniques in conjunction to improve the effectivity of the plants in controlling Fusarium wilt disease are suggested.

scholarly journals Virulensi Isolat Fusarium oxysporum f. sp. cubense VCG 01213/16 pada Pisang Barangan dari Varietas Pisang dan Lokasi yang Berbeda

2016 ◽  
Vol 21 (2) ◽  
pp. 145
Author(s):  
Jumjunidang ◽  
Catur Hermanto ◽  
Riska
Keyword(s):  
Fusarium Oxysporum ◽  
Incubation Period ◽  
Disease Severity ◽  
Control Method ◽  
Block Design ◽  
Severity Index ◽  
Disease Severity Index ◽  
Tropical Fruit ◽  
Randomized Block Design ◽  
Tissue Culture Propagation

Analisis genetik  isolat-isolat  cendawan Fusarium  oxysporum f. sp. cubense (Foc) VCG 01213/16 penyebab penyakit layu pada tanaman pisang menunjukkan adanya keragaman yang nyata. Penelitian bertujuan mempelajari keragaman virulensi isolat-isolat yang terkelompok dalam VCG 01213/16, berasal dari berbagai daerah dan varietas pisang yang berbeda.  Penelitian dilakukan di Laboratorium Penyakit dan Rumah Kasa Balai Penelitian Tanaman Buah Tropika (Balitbu Tropika) Solok, dari bulan Maret sampai dengan Juni 2009. Rancangan yang digunakan ialah acak kelompok dengan 10 perlakuan dan tiga ulangan, masing-masing perlakuan terdiri atas 10 tanaman. Perlakuan terdiri atas 10 isolat Foc VCG 01213/16 yang berasal dari varietas pisang dan lokasi berbeda. Tanaman uji ialah benih pisang Barangan hasil perbanyakan kultur jaringan. Hasil penelitian menunjukkan bahwa terdapat keragaman virulensi 10 isolat Foc VCG 01213/16 yang dinilai dari perbedaan masa inkubasi, persentase serangan, dan indeks keparahan penyakit pada bonggol dan daun pisang Barangan. Sembilan isolat Foc yang diuji mempunyai virulensi yang tinggi. Masa inkubasi berkisar antara 13,98 dan 16,80 hari, persentase serangan 93,33-100%, dan indeks keparahan penyakit pada bonggol dan daun masing-masing berkisar 3,46-5,35 dan 4,68-5,41. Isolat Foc VCG 01213/16 yang berasal dari Jabung-Lampung Timur dan diisolasi dari pisang varietas Ambon Kuning (isolat F) menunjukkan virulensi yang relatif lebih rendah dibanding sembilan isolat  Foc lainnya dengan masa inkubasi 30,27 hari, indeks keparahan penyakit pada bonggol dan daun masing-masing sebesar 2,14 dan 3,76.  Hasil penelitian ini bermanfaat dalam memberikan informasi tentang biologi F. oxysporum f. sp. cubense sebagai dasar untuk penyusunan teknik pengendalian yang tepat.<br /><br /><br />Genetic analysis of isolates of the Fusarium oxysporum f. sp. cubense (Foc) that are grouped in VCG 01213/16, as the causal agent of wilt disease in banana plants showed a considerable variation. This research aimed to study the variation in virulence of isolates that are grouped in VCG 01213/16 from different varieties of banana and regions. The study was conducted in the Protection Laboratory and the Screenhouse of Indonesian Tropical Fruit Research Institute (ITFRI) Solok, from March to June 2009. A randomized block design was used in this research with 10 treatments and three replications. Each treatments consisted of 10 banana plants. The treatment was 10 Foc isolates belonging to VCG 01213/16 originating from different varieties of banana and locations. Barangan plantlets produced from tissue culture propagation were used as the planting material. The results showed that there were high variations in virulence among 10 Foc isolates in VCG 01213/16 based on variables of the incubation period, percentage of wilt, and disease severity index on corm and leaves of Barangan variety. Nine of the 10 Foc isolates tested were highly virulent isolates. The incubation period ranged from 13.98 to 16.80 days, the percentage of wilt from 93.33 to 100%, and the disease severity index of corm and leaves ranged from 3.46 to 5.35 and from 4.68 to 5.41, respectively. The Foc VCG 01213/16 isolates originated from Jabung, East Lampung and from Ambon Kuning variety (isolate F) shown relatively low virulence than others isolates that the incubation period was 30.27 days and the disease severity index on the corm and leaves was 2.14 and 3.76, respectively. This result provides useful information on biology of F. oxysprum f. sp. cubense to find out the best control method of the pathogen.<br /><br />

scholarly journals Bio-priming of Aceh local chili seeds in the effort to increase production and begomo virus resistance

2022 ◽  
Vol 951 (1) ◽  
pp. 012081
Author(s):  
S Hafsah ◽  
Yusnizar ◽  
Nura ◽  
K S Kaloko ◽  
F Reza ◽  
...  
Keyword(s):  
Incubation Period ◽  
Disease Incidence ◽  
Block Design ◽  
Resistance Response ◽  
Disease Symptoms ◽  
Crown Width ◽  
Randomized Block Design ◽  
Two Factors ◽  
Induce Resistance ◽  
Banda Aceh

Abstract The purpose of this study was to determine whether P60 could induce resistance in local Aceh chili varieties to begomovirus and compared with national varieties. This research was conducted at the Experimental Farm, Genetics and Plant Breeding Laboratory, Faculty of Agriculture, Syiah Kuala University, Banda Aceh, from March to July 2021. This study used a factorial randomized block design. The tested factors consisted of two factors. The first factor is bio priming. The second factor consists of four local Aceh chili varieties, namely: V1 = Odeng, V2 = Lanyoe, V3 = Super Lamando and V4 = LamandoLapaben and one national variety, namely V5 = Baja F1 as a comparison variety. The results showed that the best local Aceh variety was LamandoLapaben for the variables of resistance response, incubation period, disease intensity, disease incidence, stem diameter, and crown width. The best local variety Aceh Lanyoe on plant height and dichotomous height parameters. Giving P60 can reduce the percentage of disease incidence by 52.00% and slow down the process of emergence of disease symptoms (incubation period) 27.11 DAP. The best combination treatment was the LamandoLapaben variety, which was 1.41 milligram Units-1 (Umg)-1.

PERANAN TRICHOKOMPOS DAN PUPUK KCL DALAM MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN BAWANG MERAH DI TANAH BERPASIR (The Role of Trichokompos and KCL Fertilizer to Control Fusarium Will Disease on Onion in Sandy Soil)

AgriPeat ◽  
2019 ◽  
Vol 20 (01) ◽  
pp. 19-26
Author(s):  
Admin Journal
Keyword(s):  
Research Design ◽  
Fusarium Wilt ◽  
Sandy Soil ◽  
Incubation Time ◽  
Block Design ◽  
Wilt Disease ◽  
Single Factor ◽  
Fusarium Wilt Disease ◽  
Randomized Block Design

ABSTRACTThe role of Trichocompost and KCl fertilizer to control Fusarium wilt disease on onion in sandy soil. Fusarium wilt on onion is an interesting disease it is can loss the onion yield. The purpose of research to study trichocompost and KCl fertilizer role to control Fusarium wilt disease on ann onion. The research design used a Factorial Randomized Block Design with 2 factors. The first factor is 4 levels trichocompost, it is: without trichocompost (T0), trichocompost 10 t.ha-1 dosage (T1), trichocompost 20 t.ha-1 dosage (T2), trichocompost 30 t.ha-1 dosage (T3). The second factor is 3 levels KCl fertilizer, it is: without KCl (K0), KCl 100 kg.ha-1 dosage (K1), KCl 200 kg.ha-1 dosage (K2). Result of this research showed the application of trichocompost 10 t.ha-1 dosage and KCl 100 kg.ha-1 dosage can inhibit Fusarium wilt incubation time, can inhibit the patogen development with effective value 89,23%, the single factor it is aplication trichocompost 10 t.ha-1 dosage and trichocompost 30 t.ha-1 dosage not significant to dried onion bulb weight per clump of onion plant.Key words: Trichocompost, KCl fertilizer, Fusarium wilt disease, onion, sandy soil.ABSTRAKPenyakit layu Fusarium merupakan salah satu penyakit penting dapat menurunkan produksi bawang merah hingga 50%. Tujuan penelitian untuk mengetahui peranan trichokompos dan pupuk KCl dalam mengendalikan penyakit layu fusarium pada tanaman bawang merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial dua faktor perlakuan. Faktor pertama 4 taraf dosis trichokompos yaitu: tanpa trichokompos (T0), trichokompos dosis 10 t.ha-1 (T1), trichokompos dosis 20 t.ha-1 (T2), trichokompos dosis 30 t.ha-1 (T3). Faktor kedua 3 taraf dosis pupuk KCl yaitu: tanpa pupuk KCl (K0), pupuk KCl dosis 100 KCl kg.ha-1 (K1), pupuk KCl dosis 200 KCl kg.ha-1 (K2). Hasil penelitian menunjukkan pemberian trichokompos 10 t.ha-1 dan pupuk KCl 100 kg.ha-1 dapat memperpanjang masa inkubasi penyakit, menekan serangan penyakit layu Fusarium dengan nilai efektivitas sangat baik (89,23%), perlakuan tunggal trichokompos dosis 10 t.ha-1 tidak berbeda nyata dengan dosis 30 t.ha-1 terhadap bobot umbi kering per rumpun tanaman bawang merah.Kata kunci: penyakit layu Fusarium, pupuk KCl, tanah berpasir, tanaman bawang merah, trichokompos.

scholarly journals Diallel analysis of maize resistance to Phaeosphaeria maydis

2004 ◽  
Vol 61 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Herberte Pereira da Silva ◽  
José Roberto Moro
Keyword(s):  
Disease Severity ◽  
Block Design ◽  
Genetic Resistance ◽  
Diallel Analysis ◽  
Inbred Lines ◽  
Randomized Block Design ◽  
Whole Plant ◽  
Sources Of Variation ◽  
Specific Hybrid ◽  
Additive Genetic Effects

In breeding programs directed towards genetic resistance against diseases, the estimation of genetic parameters that control resistance allows the introduction of resistance into suscetible germplasm to be clearly focused. The purposes of this study were to estimate heterosis effects, and the general (GCA) and specific (SCA) combining abilities by using two rating methods for resistance to Phaeosphaeria maydis in a diallel analysis of 36 F1 maize hybrids and their nine inbred lines. Trials were conducted in three environments. Disease severity was evaluated in the whole plant (PI) and in the leaf positioned just below the point of insertion of the main ear (AFA). The trials followed a randomized block design with plots represented by a 5 m long rows. Differences among combining ability estimates for different environments and in both evaluation methods showed significant effects (P < 0.01) for environment (E), GCA, and GCA × E. The SCA, and SCA × E effects were not significant for any of the disease severity variables. The GCA effects were more important than SCA for this set of inbred lines, suggesting that additive genetic effects are the most important sources of variation for this trait. Heterosis effects for resistance were estimated, and it was possible to identify specific hybrid combinations between lines which have high potential for genetic control of this pathogen. Results for both disease severity variables were practically identical, even though the PI method was more convenient to use.

scholarly journals The Effect of Population Density and Intercropping with Tomato on the Growth and Yield of Winged Bean (Psophocarpus tetragonolobus)

2019 ◽  
Vol 6 (02) ◽  
pp. 81-88
Author(s):  
Gerson Hans Maure ◽  
Muhammad Achmad Chozin ◽  
Edi Santosa
Keyword(s):  
Population Density ◽  
Photosynthetic Activity ◽  
Block Design ◽  
Nutrient Content ◽  
Winged Bean ◽  
Growth And Yield ◽  
Psophocarpus Tetragonolobus ◽  
Randomized Block Design ◽  
High Nutrient ◽  
Almost All

Winged bean (Fabaceae) is a tropical crop that has high nutrient content, and almost all parts of the plant are edible. The research aimed to evaluate the growth and production of winged bean in an intercropping system with tomato. The study was conducted at the Cikabayan Experimental Field of Bogor Agricultural University, Bogor, Indonesia, in a randomized block design with three replications during the rainy season of December 2017 to June 2018. Winged beans were planted in high and low population densities and intercropped with three tomato genotypes, “Tora”, F70030081-12-16-3 and “Apel Belgia”. Monoculture winged bean was assigned as control. Tomatoes were planted between the rows of the winged beans in the intercropping treatment. The results showed that the growth of winged bean in monoculture and intercropping systems was not significantly different. The photosynthetic activity of winged bean at three to five weeks after planting remained high, indicating that the vegetative growth was optimal in both systems. Intercropping increased the winged bean number of pods per plant by 12.66-19.52% compared to monoculture, irrespective of population density. Therefore, winged bean could be considered as suitable to grow in intercropping systems.

scholarly journals Kemampuan Bacillus subtilis dan Lysinibacillus sp. dalam Silika Nano dan Serat Karbon untuk Menginduksi Ketahanan Bawang Merah terhadap Penyakit Bercak Ungu (Alternaria porri (Ell.) Cif)

Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
Hersanti Hersanti ◽  
Sudarjat Sudarjat ◽  
Andina Damayanti
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Fiber ◽  
Block Design ◽  
Antagonistic Bacteria ◽  
Nano Silica ◽  
Green House ◽  
Randomized Block Design ◽  
Plant Pest ◽  
Alternaria Porri ◽  
Induce Resistance

ABSTRACTThe ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotchPurple blotch disease caused by Alternaria porri is one of the major disease on shallot. One of the methods that can be applied to control the disease is the use of antagonistic bacteria. Antagonistic bacteria can be used as a resistance inducer to suppress pathogen development. In this study, Bacillus subtilis and Lysinibacillus sp. were formulated in carbon fiber as a carrier and nano silica 3% as a complementary. This study was conducted to determine the ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotch. The experiment was conducted at the Laboratory of Phytopathology, Departement of Plant Pest and Diseases and Ciparanje Green House, Faculty of Agriculture, Universitas Padjadjaran from December 2017 until July 2018. Suspension of Bacillus subtilis and Lysinibacillus sp. singly or mixed were formulated in carbon fiber 80 Mesh and 3% nano silica. The experiment used Randomized Block Design consisted of 8 treatments with 3 replications. Each replication consisted of 5 plants. The results showed that the mixture of Bacillus subtilis and Lysinibacillus sp. in 3% silica nano and carbon fiber was the ablest treatment to increase the resistance of shallot to purple blotch by 71,2%.Keywords: Antagonistic bacteria, BiocontrolABSTRAKPenyakit bercak ungu yang disebabkan oleh Alternaria porri merupakan salah satu penyakit utama pada bawang merah. Salah satu metode yang dapat digunakan untuk pengendalian yaitu menggunakan bakteri antagonis. Bakteri antagonis dapat digunakan sebagai penginduksi ketahanantanaman untuk menekan perkembangan penyakit. Pada penelitian ini, Bacillus subtilis dan Lysinibacillus sp. diformulasikan dalam serat karbon sebagai bahan pembawa dan silika nano 3% sebagai bahan pelengkap. Percobaan ini dilakukan untuk mengetahui kemampuan bakteri Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran dalam serat karbon dan silika nano3% untuk menginduksi ketahanan bawang merah terhadap penyakit bercak ungu. Percobaan ini dilaksanakan di Laboratorium Fitopatologi, Departemen Hama dan Penyakit Tumbuhan dan Rumah Kaca, Ciparanje, Fakultas Pertanian, Universitas Padjadjaran mulai dari bulan Desember 2017 hingga Juli 2018. Suspensi Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran diformulasikan pada serat karbon dan silika nano 3%. Percobaan menggunakan rancangan acak kelompok yang terdiri dari 8 perlakuan dengan 3 ulangan. Setiap ulangan terdiri dari 5 tanaman. Dari hasil percobaan diketahui bahwa campuran B. subtilis dan Lysinibacillus sp. dalam silika nano 3% serta serat karbon mampu meningkatkan ketahanan bawang merah terhadap penyakit bercak ungu dengan persentase hambatan sebesar 71,2%.Kata Kunci: Bakteri antagonis, Biokontrol

scholarly journals EFISIENSI PENGGUNAAN Trichoderma sp UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM (Fusarium oxysporium) DAN PERTUMBUHAN BIBIT TANAMAN PISANG

2018 ◽  
Vol 14 (2) ◽  
pp. 14-28
Author(s):  
Bukhari Bukhari ◽  
Nuryulsen Safridar
Keyword(s):  
Analysis Of Variance ◽  
Fusarium Wilt ◽  
Block Design ◽  
Wilt Disease ◽  
Variance Test ◽  
Medium Intensity ◽  
Fusarium Wilt Disease ◽  
Randomized Block Design ◽  
Trichoderma Sp ◽  
Two Factors

This research was conducted in Pante Cermin, Padang Tiji Subdistrict, Pidie District. in March to August 2015, with the aim to know the use efficienci of Trichoderma sp on the growth of several types of Banana seedlings (Musa Paracica L.) This research using Randomized Block Design (RAK) factorial pattern with the factors studied is the type of banana and trichoderma dose . Both factors consist of 4 levels: Banana Barangan (J1), Ambon banana (J2), Banana King (J3) and Geupok banana (J4). While the dose of Tricoderma sp is: TO = 0 g / seeds T1 = 15 g / seeds T2 = 30 g / seedlings and T3 = 45 g / seedlings. So there are 16 treatment combinations and repeated 3 times, which resulted in 48 experimental units.To determine the influence of each treatment and its interaction on the growth of banana seedlings, the analysis of variance (Test F) and continued with the test of Beda Nyata Jujur (BNJ) at 5% level.The results showed that the growth of good banana seedlings among 4 species studied was shown by Barangan bananas, but not unlike bananas. However, trichoderma administration until the age of 4 months has not shown the growth and intensity of different attacks. So it should be extended the study period to 6 months. After 6 months of research there has been a difference in growth and intensity of attack, where the intensity of the greatest attack is shown by T0 (without trichoderma). Medium intensity of smallest attack is shown by T3 (Dose trichoderma sp 45 gr / banana seedlings). The type of banana does not affect the growth and intensity of fusarium wilt attack. and the interaction of these two factors had no significant effect on the growth of banana seedlings and the intensity of Fusarium wilt disease.

scholarly journals EFFECTIVENESS OF ANTAGONISTIC FUNGI Gliocladium sp. AND Trichoderma harzianum TO CONTROL Colletotrichum spp. on chili (Capsicum annuum L.)

Agrivet ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 17
Author(s):  
Siti Dailah ◽  
Mofit Eko Poerwanto ◽  
Supono Budi Sutoto
Keyword(s):  
Direct Method ◽  
Block Design ◽  
Fresh Weight ◽  
Antagonistic Fungi ◽  
Anthracnose Disease ◽  
Randomized Block Design ◽  
Three Samples ◽  
Colletotrichum Spp ◽  
The Mean

The productivity of chili in terms of both quality and quantity is suppressed by the attacks of anthracnose disease caused by Colletotrichum spp. Pesticides is commonly used to control the disease. Researched was conducted to determine the antagonistic fungi and the effective dose in inhibiting the growth of the Colletotrichum spp. Antagonistic tests were performed in vivo using the direct method with three replicates. Inhibition test was arranged in a Complete Randomized Block Design (RCBD) with 7 treatments: Control, Gliocladium sp (150, 250, and 350 mL/plant) and T. harzianum (150, 250, and 350 mL/plant) with four replicates and three samples out of 20 plants per treatment. The results showed that the percentage of inhibition of T. harzianum against Colletotrichum sp. is 55% while Gliocladium sp. is 70.33% in antagonistic test. The mean of disease intensity at 11 up to 32 DAI on control is 5.61 ± 0.71% up to 20.30 ± 1.35 % respectively. It is significantly higher than Gliocladium sp. 350 mL/Plant (1.94 ± 0.45 % up to 11.15 ± 0.87 %), T. harzianum 350 mL/Plant (1.10 ± 0.58 % up to 8.68 ± 0.91 %), and T. harzianum 250 mL/Plant (2.28 ± 0.43 % up to 8.75 ± 0.79 %). No significant different is observed on mean of fresh weight of red chili fruit yield per plant. T. harzianum and Gliocladium sp. fungi are effective in controlling the attack of anthracnose disease in chili. The most efficient dose to control anthracnose disease is the application of T. Harzianum or Gliocladium sp at the dose of 250 mL/plant.Key Words: Chilli, Anthracnose, Gliocladium, Trichoderma, Colletotrichum.

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