scholarly journals CHARACTERIZATIONS OF Trichoderma sp. AND ITS EFFECT ON Ralstonia solanacearum OF TOBACCO SEEDLINGS

2020 ◽  
Vol 21 (1) ◽  
pp. 8-19
Author(s):  
Sutarman Sutarman ◽  
Ahmad Khafidh Jalaluddin ◽  
Arrohmatus Syafaqoh Li’aini ◽  
Andriani Eko Prihatiningrum
Keyword(s):  
Ralstonia Solanacearum ◽  
Dry Weight ◽  
Symptom Index ◽  
In Vivo Experiments ◽  
Trichoderma Sp ◽  
Tobacco Seeds ◽  
Query Cover ◽  
Tobacco Seedlings ◽  
Trichoderma Isolates

Characterizations of Trichoderma sp. and its effect on Ralstonia solanacearum of tobacco seedlings. This study aims todetermine the molecular-based characteristics of Trichoderma sp. Tc-Jjr-02 and its effect as a biocontrol agent in protecting tobaccoseedlings against bacterial wilt caused by R. solanacearum. The characterization of biocontrol agents was based on morphologicaland molecular data’s observation using microscope and the key of determination. The in vivo experiments was consist of fivetreatments: (1) inoculation of Trichoderma isolates at six hours before R. solanacearum inoculation, (2) inoculation of Trichodermaisolates at six hours after R. solanacearum inoculation (3) simultaneous inoculation of Trichoderma isolates and R. solanacearum,(4) inoculated only with R. solanacearum, and (5) without any inoculation. The experiment was repeated six times. Based onBLAST’s analysis, the Tc-Jjr-02 sequence is in accordance with T. asperellum with 100% Query Cover. Inoculation of T. asperellumTc-Jjr-02 at six hours before and after and simultaneously with pathogens providing protection for young tobacco plants by slowingdown the time for the onset of blight by 100–162%, reducing the symptom index by 56–63%, and increasing the dry weight of plantbiomass by 39–53% compared to tobacco seeds which were only inoculated with R. solanacearum.

scholarly journals CHARACTERIZATIONS OF Trichoderma sp. AND ITS EFFECT ON Ralstonia solanacearum OF TOBACCO SEEDLINGS

2020 ◽  
Vol 21 (1) ◽  
pp. 8-19
Author(s):  
Sutarman Sutarman ◽  
Ahmad Khafidh Jalaluddin ◽  
Arrohmatus Syafaqoh Li’aini ◽  
Andriani Eko Prihatiningrum
Keyword(s):  
Plant Biomass ◽  
Dry Weight ◽  
Symptom Index ◽  
In Vivo Experiments ◽  
Trichoderma Sp ◽  
Tobacco Seeds ◽  
Query Cover ◽  
Tobacco Seedlings ◽  
Trichoderma Isolates

This study aims to determine the molecular-based characteristics of Trichoderma sp. Tc-Jjr-02 and its effect as a biocontrol agent in protecting tobacco seedlings against bacterial wilt caused by R. solanacearum. The characterization of biocontrol agents was based on morphological and molecular data’s observation using microscope and the key of determination. The in vivo experiments was consist of five treatments: (1) inoculation of Trichoderma isolates at six hours before R. solanacearum inoculation, (2) inoculation of Trichoderma isolates at six hours after R. solanacearum inoculation (3) simultaneous inoculation of Trichoderma isolates and R. solanacearum, (4) inoculated only with R. solanacearum, and (5) without any inoculation. The experiment was repeated six times. Based on BLAST’s analysis, the Tc-Jjr-02 sequence is in accordance with T. asperellum with 100% Query Cover. Inoculation of T. asperellum Tc-Jjr-02 at six hours before and after and simultaneously with pathogens providing protection for young tobacco plants by slowing down the time for the  onset of blight by 100–162%, reducing the symptom index by 56–63%, and increasing the dry weight of plant biomass by 39–53% compared to tobacco seeds which were only inoculated with R. solanacearum.

scholarly journals Control of lettuce bottom rot by isolates of Trichoderma spp

2014 ◽  
Vol 40 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Zayame Vegette Pinto ◽  
Matheus Aparecido Pereira Cipriano ◽  
Amaury da Silva dos Santos ◽  
Ludwig Heinrich Pfenning ◽  
Flávia Rodrigues Alves Patrício
Keyword(s):  
Mycelial Growth ◽  
High Capacity ◽  
Dry Weight ◽  
Second Step ◽  
Trichoderma Spp ◽  
In Vivo Experiments ◽  
Bottom Rot ◽  
Trichoderma Isolates

Bottom rot, caused by Rhizoctonia solani AG 1-IB, is an important disease affecting lettuce in Brazil, where its biological control with Trichoderma was not developed yet. The present study was carried out with the aim of selecting Trichoderma isolates to be used in the control of lettuce bottom rot. Forty-six Trichoderma isolates, obtained with baits containing mycelia of the pathogen, were evaluated in experiments carried out in vitro and in vivo in a greenhouse in two steps. In the laboratory, the isolates were evaluated for their capabilities of parasitizing and producing toxic metabolic substances that could inhibit the pathogen mycelial growth. In the first step of the in vivo experiments, the number and the dry weight of lettuce seedlings of the cultivar White Boston were evaluated. In the second step, 12 isolates that were efficient in the first step and showed rapid growth and abundant sporulation in the laboratory were tested for their capability of controlling bottom rot in two repeated experiments, and had their species identified. The majority of the isolates of Trichoderma spp. (76%) showed high capacity for parasitism and 50% of them produced toxic metabolites capable of inhibiting 60-100% of R. solani AG1-IB mycelial growth. Twenty-four isolates increased the number and 23 isolates increased the dry weight of lettuce seedlings inoculated with the pathogen in the first step of the in vivo experiments.In both experiments of the second step, two isolates of T. virens, IBLF 04 and IBLF 50, reduced the severity of bottom rot and increased the number and the dry weight of lettuce seedlings inoculated with R. solani AG1-IB. These isolates had shown a high capacity for parasitism and production of toxic metabolic substances, indicating that the in vitro and in vivo steps employed in the present study were efficient in selecting antagonists to be used for the control of lettuce bottom rot.

scholarly journals Effect of Allium fistulosum Extract on Ralstonia solanacearum Populations and Tomato Bacterial Wilt

Plant Disease ◽  
2012 ◽  
Vol 96 (5) ◽  
pp. 687-692 ◽  
Author(s):  
Péninna Deberdt ◽  
Benjamin Perrin ◽  
Régine Coranson-Beaudu ◽  
Pierre-François Duyck ◽  
Emmanuel Wicker
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Management Strategies ◽  
Antimicrobial Effect ◽  
Allium Fistulosum ◽  
Inhibition Assay ◽  
In Vivo Experiments ◽  
Tomato Bacterial Wilt

To control bacterial wilt (Ralstonia solanacearum, phylotype IIB/4NPB), the antimicrobial effect of Allium fistulosum aqueous extract was assessed as a preplant soil treatment. Three concentrations of extract (100, 50, and 25%, 1:1 [wt/vol]) were evaluated by in vitro inhibition assay and in vivo experiments in a growth chamber. In vitro, A. fistulosum (100 and 50%) suppressed growth of R. solanacearum. Preplant treatment of the soil with A. fistulosum extract significantly reduced the R. solanacearum populations. No pathogen was detected in the soil after treatment with 100% concentrated extract from the third day after application until the end of the experiment. A. fistulosum also significantly reduced the incidence of tomato bacterial wilt. In the untreated control, the disease affected 61% of the plants whereas, with 100 and 50% extracts, only 6 and 14% of the plants, respectively, were affected. These results suggest that A. fistulosum extracts could be used in biocontrol-based management strategies for bacterial wilt of tomato.

scholarly journals Mercury detoxification using genetic engineered Nicotiana tabacum

2013 ◽  
Vol 10 (3) ◽  
pp. 432-438
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Inorganic Mercury ◽  
Dry Weight ◽  
Mercury Resistance ◽  
Wild Type ◽  
Mercury Compounds ◽  
Tobacco Seeds ◽  
Tobacco Seedlings ◽  
Mercury Detoxification

Phytoremediation is a low cost alternative solution to soil contamination compared with traditional removal and/or disposal techniques. One of the phytoremediation technologies is the phytovolatilization, whereby the contaminant is not primarily accumulated in above-ground tissues, but is instead transformed by the plant into the atmosphere. The detoxification of highly toxic organmercurial compounds and subsequent volatilization of elemental mercury is a unique example for the successful phytoremediation based on genetic engineering approach. For mercury removal techniques we constructed a dicistronic construct containing the bacterial mercury detoxification genes merA and merB under the control of the Arabidopsis Actin2 promoter and terminator. The resulted construct was introduced to Agrobacterium competent cells using heat shock transformation method. In the mean time, we germinated tobacco (Nicotiana tabacum var. Gold leaf) seeds on MS media and infected leaf discs of 6-8 weeks tobacco seedlings with Agrobacterium containing Ti plasmid harboring merA/merB dicistronic construct. The results showed that about 90% of tobacco seedlings were carrying the mer genes. Tobacco seeds were collected from wild type and transgenic lines and tested for mercury resistance. The results showed that transgenic plants are resistant to both Phenyl Mercuric Acetate (PMA) and HgCl2. The root length and dry weight of wild and transgenic seedlings growing on both media amended with mercury compounds and media without mercury (control) were scored. The results showed that the root lengths and dry weight of the transgenic lines are significantly higher by 60 and 17-folds, respectively, compared to wild type. The results showed clear evidence that the transgenic plants are resistant to both organic and inorganic mercury compounds and can be used to clean up mercury contaminated sites.

Stem Cell Cure for Kidney Injury: Therapy to Solve Most Complex Issues in Renal System

2020 ◽  
Author(s):  
Prithiv K R Kumar
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Kidney Injury ◽  
Cell Types ◽  
Tissue Remodelling ◽  
Major Health Problem ◽  
In Vivo Experiments ◽  
Renal Regeneration ◽  
Induced Pluripotent

Renal failure is a major health problem. The mortality rate remain high despite of several therapies. The most complex of the renal issues are solved through stem cells. In this review, different mechanism for cure of chronic kidney injury along with cell engraftment incorporated into renal structures will be analysed. Paracrine activities of embryonic or induced Pluripotent stem cells are explored on the basis of stem cell-induced kidney regeneration. Several experiments have been conducted to advance stem cells to ensure the restoration of renal functions. More vigour and organised protocols for delivering stem cells is a possibility for advancement in treatment of renal disease. Also there is a need for pressing therapies to replicate the tissue remodelling and cellular repair processes suitable for renal organs. Stem cells are the undifferentiated cells that have the ability to multiply into several cell types. In vivo experiments on animal’s stem cells have shown significant improvements in the renal regeneration and functions of organs. Nevertheless more studies show several improvements in the kidney repair due to stem cell regeneration.

In vivo Experiments of Natural Products Protection of Antagonistic Effects of Lead on Iron

2018 ◽  
Vol 68 (12) ◽  
pp. 2747-2751
Author(s):  
Marioara Nicula ◽  
Nicolae Pacala ◽  
Lavinia Stef ◽  
Ioan Pet ◽  
Dorel Dronca ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Iron Homeostasis ◽  
Iron Concentration ◽  
Antagonistic Effect ◽  
Tissue Samples ◽  
Antagonistic Effects ◽  
In Vivo Experiments ◽  
Living Organisms ◽  
Toxic Potential

Living organisms take nutrients from the environment, and together with them, substances with toxic potential � such as heavy metals. Lead is one common metal pollutant especially in aquatic environment, from where the fish can be intoxicated very easily. Bioavailability, distribution, toxic action, synergistic and antagonistic effects are characteristics which can alter the fish health. Our experimental study followed the effects of lead overload in water on iron distribution, in different tissues sample Carassius gibelio Bloch fish. We performed the experiment in four different fish groups: control C; lead � Pb (administration of lead in water 0.075mg/mL of water, as Pb(NO3)2 x � H2O); lead (the same dose) and 2% of freeze-dry garlic incorporated into fishes� food � Pb+garlic; lead (the same dose) and 2% chlorella incorporated into fishes� food � Pb+chlorella, for 21 consecutive days. The iron concentration was analysed with AAS (Atomic Absorption Spectroscopy) from gills, muscle, skin (and scales), intestine, liver, heart, brain, ovary, testicles, and kidney. The obtained data presented a significantly decrease of iron content in all tested tissue samples that demonstrated, alteration of iron homeostasis, explained by a strong antagonistic effect of lead on iron. Our experiment showed that biologic active principles from garlic and chlorella act like natural protectors, and potentiate the iron deficiency even in the case of lead overload in aquatic environment, for fish.

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

Human Fibronectin Extra-Domain B (EDB)-Specific Aptide (APTEDB) Radiolabelling with Technetium-99m as a Potent Targeted Tumour-Imaging Agent

2018 ◽  
Vol 18 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Mohsen Mohammadgholi ◽  
Nourollah Sadeghzadeh ◽  
Mostafa Erfani ◽  
Saeid Abediankenari ◽  
Seyed Mohammad Abedi ◽  
...  
Keyword(s):  
Radioligand Binding ◽  
Specific Binding ◽  
Specific Protein ◽  
Tumour Imaging ◽  
Specific Binding Ratio ◽  
Tumour Uptake ◽  
Technetium 99M ◽  
In Vivo Experiments ◽  
Human Fibronectin

Background: Human fibronectin extra-domain B (EDB) is particularly expressed during angiogenesis progression. It is, thus, a promising marker of tumour growth. Aptides are a novel class of peptides with high-affinity binding to specific protein targets. APTEDB is an antagonist-like ligand that especially interacts with human fibronectin EDB. Objective: This study was the first attempt in which the hydrazinonicotinamide (HYNIC)-conjugated APTEDB was labelled with technetium-99m (99mTc) as an appropriate radiotracer and tricine/EDDA exchange labeling. Methods: Radiochemical purity, normal saline, and serum stability were evaluated by HPLC and radio-isotope TLC scanner. Other examinations, such as protein-binding calculation, dissociation radioligand binding assay, and partition coefficient constant determination, were also carried out. The cellular-specific binding of 99mTc- HYNIC-conjugated APTEDB was assessed in two EDB-positive (U87MG) and EDB-negative (U373MG) cell lines. Bio-distribution was investigated in normal mice as well as in U87MG and U373MG tumour-bearing mice. Eventually, the radiolabelled APTEDB was used for tumour imaging using planar SPECT. Results: Radiolabelling was achieved with high purity (up to 97%) and accompanied by high solution (over 90% after overnight) and serum (80% after 2 hours) stability. The obtained cellular-specific binding ratio was greater than nine-fold. In-vivo experiments showed rapid blood clearance with mainly renal excretion and tumour uptake specificity (0.48±0.03% ID/g after 1h). The results of the imaging also confirmed considerable tumour uptake for EDB-positive cell line compared with the EDB-negative one. Conclusion: Aptides are considered to be a potent candidate for biopharmaceutical applications. They can be modified with imaging or therapeutic agents. This report shows the capability of 99mTc-HYNIC-APTEDB for human EDB-expressing tumours detection.

Passive Cavitation Mapping by Cavitation Source Localization from Aperture-Domain Signals - Part 2: Phantom and in vivo Experiments

2020 ◽  
pp. 1-1
Author(s):  
Arsenii V. Telichko ◽  
Taehwa Lee ◽  
Dongwoon Hyun ◽  
Sayan M. Chowdhury ◽  
Sunitha Bachawal ◽  
...  
Keyword(s):  
Source Localization ◽  
In Vivo Experiments
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