Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of  Deteriorated Soybean Seed

Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integrated with rhizobateri to viability and vigor of deteriorated soybean seed. A completely randomized design with four replicates were used in this experiment. Seeds were treated with varied combination of hydration period in a rhizobacteria suspension and followed by dehydration time. Data were analysed using the F-test and significant differences were further tested with Least Significant Difference at the 5% level. Results showed that the hydration and dehydration period of seeds in a rhizobacteria suspension improved viability and vigor of seed which have 59.5% initial germination percentage. The improvement was 12.28%, 0.56, 25.4% and 1.4 days for standard germination test, vigor index, first count test percentage and T50, respectively. The hydration for 60 minutes followed by dehydration for 60 minutes gave the best results on seed viability and vigor.

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The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v7i1.18 ◽

2020 ◽

Vol 7 (1) ◽

pp. 62-66

Author(s):

Masniar Vina Kesti ◽

Bambang Irawan ◽

Priyambodo Priyambodo ◽

Martha Lulus Lande

Keyword(s):

Dry Weight ◽

Growing Media ◽

Randomized Design ◽

Vigor Index ◽

Growing Medium ◽

Significant Difference ◽

Seedling Roots ◽

Completely Randomized Design ◽

Water Gas ◽

Difference Test

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.

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Pengaruh Dosis Minyak Cengkeh Terhadap Populasi Hama Callosobruchus chinensis L., Viabilitas Dan Vigor Benih Dua Kultivar Kacang Hijau Setelah Periode Simpan Tiga Bulan

Agrologia ◽

10.30598/a.v7i1.353 ◽

2018 ◽

Vol 7 (1) ◽

Author(s):

Siti Syarah Nurbaekah ◽

Sumadi Sumadi ◽

Anne Nuraini

Keyword(s):

Mung Bean ◽

Germination Rate ◽

Seed Viability ◽

Seed Vigor ◽

Callosobruchus Chinensis ◽

Clove Oil ◽

Bean Cultivar ◽

Randomized Design ◽

Vigor Index ◽

Completely Randomized Design

Callosobruchus chinensis L. still become the primary pest of mung bean, it can cause damage until 70 percent. Pest control on mung bean seeds can be done by using anorganic protectant and bio protectant. The aim of the research was to determine the dosage of clove oil that could suppressed the population of weevil C. chinensis L. and maintaining seed viability and seed vigor of two mung bean cultivar after three months storage. The experimental design was Completely Randomized Design with 10 treatments combination of cultivar and clove oil dosage; all treatment were replicated three times. The mungbean seeds were cultivar Vima 1 and Vima 3. The clove oil dosages used were 0 mL kg-1, 0,5 mL kg-1, 1,0 mL kg-1, 1,5 mL kg-1 and 2,0 mL kg-1. The mungbean result of experiment showed that there were significant effect between cultivar and clove oil dosage on germination rate percentage after three months storage, but no significant effect on weevil population and vigor index. The dosage of 1,5 mL kg-1 clove oil was the better treatment to maintaining seed viabilty andits vigor.Keywords: Mung bean, C. chinensis L., Clove oil, Seed viability and Seed Vigor.

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Germination and Viability of Seeds of Caesalpinia pulcherrima Newly Harvested and Stored

Journal of Agricultural Science ◽

10.5539/jas.v11n7p73 ◽

2019 ◽

Vol 11 (7) ◽

pp. 73

Author(s):

Janaina S. Ferro ◽

Dougllas F. Rocha ◽

João Luciano A. M. Junior ◽

João C. A. Neto ◽

José V. Silva ◽

...

Keyword(s):

Germination Percentage ◽

Water Volume ◽

Tetrazolium Salt ◽

Shrub Species ◽

Viability Analysis ◽

Randomized Design ◽

Significant Difference ◽

Medicinal Properties ◽

Completely Randomized Design ◽

Temperature Storage

Caesalpinia pulcherrima is an exotic shrub species, belonging to the Fabaceae family, that has medicinal properties, and is widely used for urban afforestation. The objective of this research was to evaluate the overcoming of the C. pulcherrima seed dormancy, the influence of temperature, storage, and water quantity on the substrate in the germination of the species, as well as the use of the tetrazolium test for viability analysis. The analyzes were carried out at the Plant Propagation Laboratory at the Agricultural Sciences Center of the Federal University of Alagoas, in the municipality of Rio Largo, Brazil, and at the Laboratory of Plant Physiology, Arapiraca Campus, at the same University. The experiments were performed under a completely randomized design, with four replicates of 25 seeds. The results demonstrated that during storage the seeds developed a possible secondary dormancy, which was overcome with a time of twelve months of storage. The C. pulcherrima seeds subjected to the test of light qualities presented a significant difference in their germination percentage when verified with the time of storage. In the far-red quality, the newly harvested seeds had a germination percentage of (98%), higher than seeds with 12 months of storage (80.5%). The water volume 3.5-fold the weight of the dry paper provides (90%) germination when compared to other volumes. The tetrazolium salt concentrations of 0.075% and 0.1%, under the temperature of 30°C within 2 hours, are indicated for the viability analysis of Caesalpinia pulcherrima seeds.

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Pengaruh Perendaman Benih Pada Berbagai Suhu Awal Air Terhadap Viabilitas Benih Kayu Afrika (Maesopsis Eminii)

Jurnal Sylva Lestari ◽

10.23960/jsl12101-108 ◽

2014 ◽

Vol 2 (1) ◽

pp. 101

Author(s):

Oben . ◽

Afif Bintoro ◽

Melya Riniarti

Keyword(s):

Seed Dormancy ◽

Initial Temperature ◽

Seed Viability ◽

Germination Percentage ◽

Temperature Treatment ◽

Randomized Design ◽

Series Of Experiments ◽

Completely Randomized Design ◽

Maesopsis Eminii ◽

Tree Seed

One of thepropagation problems in generative of umbrella treewas seed dormancy. To break the seed dormancy of umbrella tree seed, soaking at different initial temperatures of water was used. The objectives of this research were to find out the effect of initial temperature treatment to the germination percentage, the germination speed rate, and the ability to germinate, and also to find the optimum initial temperature for umbrella tree germination. The research hasstartedFebruary until May 2012 in PT. Anugerah Subur Sejahtera, Ulak Bandung Village, sub-districtofMuara Sahung, Kaur Regency, Bengkulu.This research used completely randomized design (CRD) with 4 treatment and 3 replications, each series of experiments used 100 seeds.The result shown that soaking treatment at different initial temperature of water has been significantly affected the germination percentage, and the umbrella tree seed without soaking gave the best result. Key words : germination, scarification, seed viability, umbrella tree

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LACK OF EFFECT OF PRETREATMENT ON THE VIABILITY OF MACADAMIA (Macadamia integrifolia) SEED

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v3n2.2002.58-61 ◽

2013 ◽

Vol 3 (2) ◽

pp. 58 ◽

Cited By ~ 2

Author(s):

Maharani Hasanah ◽

Sukarman Sukarman ◽

Devi Rusmin ◽

Devi Rusmin

Keyword(s):

Seed Germination ◽

Seed Viability ◽

Germination Percentage ◽

Macadamia Integrifolia ◽

Randomized Design ◽

Dehydrogenase Enzyme ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Good Quality Seed ◽

Dehydrogenase Enzyme Activity

One problem in developing macadamia in Indonesia is the limited availability of good quality seed. Viability of the seed is easily lost and erratic, and the seed has long dormancy periods. The objective of the study was to find a method for enhancing germination percentage of macadamia seed. The study evaluated different pretreatments of macadamia seeds before planting. The pretreatments were period of fermentation, i.e., 0, 1, 2, 3, 4, 5, 6, and 7 daysone day each soakingdrying-soakingone day each drying-soaking-dryingand alternate drying-soaking until the seeds were cracked. The treatments were arranged in completely randomized design. Results showed that no treatment significantly affected percentage of seed germination, index of speed of germination, number of leaves, and dehydrogenase enzyme activity, but treatments significantly affected seedling height and electric conductivity of seed leakage solution. Seed germination percentage ranged from 66.67 to 86.67.

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The Effect of Dormancy Breakdown through Water Immersion with Temperature Stratification on the Germination of Sugar Palm Seeds (Arenga pinnata)

Jurnal Sylva Lestari ◽

10.23960/jsl1877-84 ◽

2020 ◽

Vol 8 (1) ◽

pp. 77

Author(s):

Astry Sri Rezeki Rumahorbo ◽

Duryat . ◽

Afif Bintoro

Keyword(s):

Germination Rate ◽

Water Immersion ◽

Germination Percentage ◽

Germination Test ◽

Physical Dormancy ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

C 50 ◽

Control Water

Sugar palm (Arenga pinnata) has a hard and impermeable pericarp, which make an obstacle for it’s germination. Scarification of seed is needed to breakdown the physical dormancy of seeds. One of the methods to break the physical dormancy is stratification by soaking seed in to the water in some different temperature level. The aims of the study were to determine the effectiveness of stratification and to figure out the best water temperature in breaking the dormancy. A Completely Randomized Design (CRD) in four treatments (control, water immersion at 25°C, 50°C, and 75°C) with four replications was employed as research design. Statistical analysis was conducted using homogenity of variance, variance analysis, and least significant difference tests. The result showed that the water immersion of arenga seed at 25°C and 50°C resulted in the germination percentage, germination rate and test germination that was comparable to control. Water immersion at 75°C reduced the percentage of germination and germination rate, however the treatment could increase the germination test. Water immersion of arenga seed at 25°C, 50°C, and 75°C were not effective to break the dormancy of sugar palm seed.Keywords: aren seeds, dormancy, water immersion

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Estimation of the cardinal temperatures for germination of four Satureja species growing in Iran

Herba Polonica ◽

10.1515/hepo-2016-0001 ◽

2016 ◽

Vol 62 (1) ◽

pp. 7-21 ◽

Cited By ~ 1

Author(s):

Hamed Zarif Ketabi ◽

Hamid Reza Khazaei ◽

Ahmad Nezami ◽

Seyed Reza Tabaei Aghdaei

Keyword(s):

Regression Models ◽

Germination Rate ◽

Germination Percentage ◽

Randomized Design ◽

Significant Difference ◽

Mean Germination Time ◽

Completely Randomized Design ◽

Genetic And Environmental Factors ◽

Cardinal Temperatures ◽

Temperature Water

SummaryIntroduction Seed germination is a complex physiological process regulated by genetic and environmental factors including temperature, water, oxygen, light and pH. Among them, temperature is one of the most important factors controlling the maximum rate and percentage of diaspore germination. Objective: The aim of the study was to determine the cardinal temperatures (Tb, To, Tc) of four Satureja species growing in Iran. Methods: Seeds of Satureja mutica Fish. et C. A. Mey., S. macrantha C. A. Mey., S. sahandica Bornm and S. bachtiarica Bunge were germinated at nine constant temperatures (from 0 to 40°C) with 5°C intervals. A factorial experiment based on completely randomized design with four replications was applied to determine the cardinal temperatures estimated by three regression models including intersected-lines (ISL), quadratic polynomial (QPN) and five-parameters beta (FPB). Results: The highest germination percentage (GP) occurred at 20°C for S. mutica (86%), S. macrantha (55%), S. sahandica (81%) and S. bachtiarica (89%), but there was no significant difference between 20 and 25°C, except S. sahandica. Germination stopped at 0°C and 40°C. The highest germination rate (GR), the lowest mean germination time (MGT) and time to 50% germination (D50) were obtained at 20-25°C for all species. The GRmfor S. bachtiarica was significantly (p≤0.05) higher than for three other species in all temperatures. None of the species did reach to 50% germination at temperatures higher than 30°C. Conclusion: Obtained results revealed the superiority of S. bachtiarica over the other species, v.s. S. macrantha was inferior. FPB and ISL models were most reliable for predicting cardinal temperatures, because of higher R2value and the lower root mean square error (RMSE). S. macrantha and S. mutica showed the lowest and the highest cardinal temperatures, respectively, in all three models.

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Effect of planting date and density on calendula and peppermint herbs

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v1i1.4241 ◽

2014 ◽

Vol 1 (1) ◽

pp. 25-29

Author(s):

Rahim Mohammadian ◽

Behnam Tahmasebpour ◽

Peyvand Samimifar

Keyword(s):

Mutual Effect ◽

Dry Weight ◽

Planting Date ◽

Mass Reduction ◽

Flower Number ◽

Planting Dates ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Total Maximum

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

Annals of Botany ◽

10.1093/aob/mcab009 ◽

2021 ◽

Author(s):

Filippo Guzzon ◽

Maraeva Gianella ◽

Jose Alejandro Velazquez Juarez ◽

Cesar Sanchez Cano ◽

Denise E Costich

Keyword(s):

Seed Germination ◽

Plant Genetic Resources ◽

Seed Viability ◽

Storage Conditions ◽

Seed Longevity ◽

Germplasm Bank ◽

Significant Difference ◽

Different Temperatures ◽

Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

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