scholarly journals GC-MS Analysis of Bioactive Compounds in Some Wild-Edible Mushrooms from Calabar, Southern Nigeria

Author(s):  
Juliet O. Oni ◽  
Ferdinand A. Akomaye ◽  
Aniedi-Abasi A. Markson ◽  
Augustine C. Egwu

Studies to exploit wild mushrooms as a source of biologically active compounds are gaining importance in the recent years. In that view, the present study was carried out to evaluate the bioactive compounds of six wild edible mushrooms: Lentinus squarrosulus Mont., Auricularia auricular-judae (Bull.) Wettst., Mycetinis copelandii (Desjardin) A.W. Wilson & Desjardin, Baeospora myosura (Fr.) Singer, Pleurotus ostreatus (Jacq. ex. fr) Kummer and Volvariella volvacea (Bull. ex. Fr.) Singer. The specimen was subjected to phytochemical screening using gas chromatography mass spectrometer (GCMS) with the view to identify the important volatile constituents. The dried sporocarp was subjected to methanol extraction using a Soxhlet extractor and rotatory evaporator. This led to the identification of 14, 26, 33, 5, 49 and 32 different compounds in L. squarrosulus, A. auricular-judae, M. copelandii, B. myosura, P. ostreatus and V. volvacea respectively. n-Hexadecanoic acid; 9,12-Octadecadienoic acid (Z,Z)- and 2(3H)-Furanone where the most frequently occurring compounds across the studied mushrooms. The identified compound reportedly shows diverse bioactive activities, including antiviral, anticancer, antimicrobial, antioxidant, hypocholesterolemic, anaphylactic, narcotic, neurostimulant, emollient, expectorant, laxative, pesticidal, insecticidal and insectifugal activities. This data may provide baseline information about the bioactive constituents of these species from Nigeria, in providing valuable compounds of substantial medicinal and agricultural importance.

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2996
Author(s):  
Krishan Kumar ◽  
Rahul Mehra ◽  
Raquel P. F. Guiné ◽  
Maria João Lima ◽  
Naveen Kumar ◽  
...  

Mushrooms are well-known functional foods due to the presence of a huge quantity of nutraceutical components. These are well recognized for their nutritional importance such as high protein, low fat, and low energy contents. These are rich in minerals such as iron, phosphorus, as well as in vitamins like riboflavin, thiamine, ergosterol, niacin, and ascorbic acid. They also contain bioactive constituents like secondary metabolites (terpenoids, acids, alkaloids, sesquiterpenes, polyphenolic compounds, lactones, sterols, nucleotide analogues, vitamins, and metal chelating agents) and polysaccharides chiefly β-glucans and glycoproteins. Due to the occurrence of biologically active substances, mushrooms can serve as hepatoprotective, immune-potentiating, anti-cancer, anti-viral, and hypocholesterolemic agents. They have great potential to prevent cardiovascular diseases due to their low fat and high fiber contents, as well as being foremost sources of natural antioxidants useful in reducing oxidative damages. However, mushrooms remained underutilized, despite their wide nutritional and bioactive potential. Novel green techniques are being explored for the extraction of bioactive components from edible mushrooms. The current review is intended to deliberate the nutraceutical potential of mushrooms, therapeutic properties, bioactive compounds, health benefits, and processing aspects of edible mushrooms for maintenance, and promotion of a healthy lifestyle.


Author(s):  
Sayed Rashad ◽  
Ghadir El-Chaghaby

Nowadays, the use of natural bio-products in pharmaceuticals is gaining popularity as safe alternatives to chemicals and synthetic drugs. Algal products are offering a pure, healthy and sustainable choice for pharmaceutical applications. Algae are photosynthetic microorganisms that can survive in different environmental conditions. Algae have many outstanding properties that make them excellent candidate for use in therapeutics. Algae grow in fresh and marine waters and produce in their cells a wide range of biologically active chemical compounds. These bioactive compounds are offering a great source of highly economic bio-products. The present review discusses the phytochemical and bioactive compounds present in algae biomass and their potent biological activities. The review focuses on the use of alga in therapy and their pharmaceutical applications with special reference to the possible preventive and therapeutic role of algae against COVID-19.


Author(s):  
R. Gaglio ◽  
A. Saitta ◽  
M. Cruciata ◽  
A. La Rosa ◽  
P. Barbaccia ◽  
...  

Background: The continuous worldwide increase of consumption of fresh mushrooms has registered in the recent years. The major goal of this study was to determine the microbiological characteristics of wild edible mushrooms and effect of temperature during storage of Morchella conica. Methods: Wild mushrooms of the species Boletus edulis, Cantharellus cibarius, and Leccinum aurantiacum were collected in a mixed forest of Picea abies, Betula pendula, and Pinus sylvestris located in Tartu territory, Estonia. Faecal indicators, potential pathogens, spoilage bacteria, and microfungi (yeasts and moulds) were evaluated. M. conica was microbiologically investigated for 24 days under different thermal regimes, including 4, 8, 12, 15, 20, and 28 °C. The statistical analysis was conducted with SAS 9.2 software. Results: The microbial counts of wild mushrooms, ranging from 6.81 to 7.68 log10 CFU/g for total mesophilic count, were generally higher (p<0.05) than those registered for marketed samples ranging from 4.60 to 7.39 log10 CFU/g. The dynamics of total mesophilic microorganisms on M. conica stored at different temperatures indicated that stationary and death phases occurred earlier with increasing temperature and that the highest levels were registered at 28 °C at the 2nd day of storage. Conclusion: This work highlights consistent differences in terms of microbiological properties among different mushrooms species. The results clearly showed that total mesophilic populations developed at high levels quickly at temperatures more than 15 °C, but also the refrigeration did not stop the microbiological decay of mushrooms.


2014 ◽  
Vol 490-491 ◽  
pp. 142-145 ◽  
Author(s):  
Ying Zhang ◽  
Ge Yang

Two heavy metals (nickel and cadmium ) in 15 specis of wild mushrooms were determined using flame atomic adsorption spectrometry (VARIAN, SpectrAA-220Fs). The mushrooms were collected from Miyi, China. The study explored the accumulation of heavy metals in different macrofung. It is found that the range of Nickel and Cadmium in mushrooms were 1.78-21.22 mg/kg and 0.47-21.84 mg/kg. Minimal and maximal concentrations of heavy metals were determined in Lentinus edodes and Boletus edulis for nickel, Russula alutacea and Russula delica for cadmium,respectively. The order of the levels of heavy metals in the mushrooms samples was found to be as Ni>Cd. Cadmium values in wild mushrooms collected from Miyi were generally higher than the previous studies. Nickel contents were in agreement with the literature value.


2011 ◽  
Vol 17 (6) ◽  
pp. 557-567 ◽  
Author(s):  
D. Kumari ◽  
M.S. Reddy ◽  
R.C. Upadhyay

A total of 18 wild edible mushrooms of Cantharellus species were collected from northwestern Himalayan region of India. The basic composition (moisture, total carbohydrates, dietary fiber, crude fat, ash, nitrogen and protein) and amino acid contents (by high-performance liquid chromatography) of these wild edible mushrooms were determined. The macronutrient profile in general revealed that the wild mushrooms were rich sources of protein and carbohydrates, and had low amounts of fat. Total phenolics and antioxidant activity from water and methanolic extracts of these mushrooms were also determined. These wild mushrooms also had significant amount of phenol content and antioxidant capacity. Studies also provide the precise antioxidant status of 18 indigenous species of mushrooms, which can serve as a useful database for the selection of mushrooms for the function of preparation of mushroom-based nutraceutics.


2014 ◽  
Vol 884-885 ◽  
pp. 611-614
Author(s):  
Ying Zhang ◽  
Shao Juan Jiang ◽  
Ge Yang

The aim of the present study was to analyze and compare contents of copper, zinc and chromium in 15 kinds of wild mushrooms. heavy metals were determined using flame atomic adsorption spectrometry (VARIAN, SpectrAA-220Fs). The mushrooms were collected from Miyi, China. The study explored the accumulation of heavy metals in different macrofung. Minimal and maximal concentrations of heavy metals were determined in Termitomyces microcarpus and Amanita esculenta for copper, Ramaria flava and Termitomyces for zinc, Hygrophorus eburnesus and Boletus aereus for chromium ,respectively. chromium values in wild mushrooms collected from Miyi were generally higher than the previous studies. Copper and zinc contents were in agreement with the literature value.


2021 ◽  
Vol 27 (4) ◽  
pp. 4157-4163
Author(s):  
Tsonka Dimitrova ◽  
◽  
Petko Marinov ◽  
Galina Yaneva ◽  
Dobri Ivanov ◽  
...  

Purpose: The interest in evaluation of real knowledge of wild edible mushrooms at individual and population level increases mainly in developing countries. The purpose of our inquiry study was to assess the level of knowledge and awareness of the wild mushrooms in a random sample from the population of Varna region in Bulgaria. Material/Methods: We performed an anonymous questionnaire investigation of 200 adult individuals, 100 males and 100 females, from Varna region, including 17 potential opinions from their personal knowledge of wild edible mushroom usage. Statistical data processing was performed using descriptive methods, variation and correlation analyses. Results: Most respondents acquired their knowledge of wild edible mushrooms from their relatives. From all 200 persons investigated 78 respondents were not capable of recognizing at least one wild edible mushroom at all. More respondents trusted other people, who have very good experience with preparation of wild mushrooms, thus relying on differentiation of a mushroom poison. Their awareness was associated with their capacity to properly recognize wild mushrooms as well as their long-term practice to recognize toxic mushrooms. Just a minority of respondents shared that wild mushrooms in their vicinity could be a problem and had a slight idea of mycotherapy. Only two females had heard about training for wild mushroom recognition. There were statistically significant correlation dependences between respondent’s awareness of wild edible mushrooms, on one hand, and individual sex status, educational level and main residence, on the other hand. Conclusion: Our study identified two most recognized wild edible mushroom species in Varna region, edible boletus (Boletus edulis Bull) and parasol mushroom (Macrolepiota procera S. F. Gray). Most respondents acquired their knowledge of wild edible mushrooms from relatives. A problem-oriented training of the population on this topic should be performed to improve awareness of common mushroom features and avoid mushroom poisoning.


Jurnal Wasian ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 121-135
Author(s):  
Ivan Permana ◽  

Belitong Island, The Province of Bangka Belitung is one of the exotic places with the biodiversity which has not been optimally inventoried. One of the potential germplasm that has not been recorded properly on Belitong Island is the mushrooms diversity and their potential uses. Mushroom are known as non-timber forestry product that is often sought after by local ethnic of Belitong Island and used as food. Apart from being consumed, these wild mushrooms are also traded, so they are one of the important seasonal commodities for the local community. This research aimed to provide the basic taxonomical information of wild edible mushroom in Belitong island as well as the biological characters. Observations were conducted 2 times in 2018 – 2019 using opportunistic sampling method.The utilization data of wild edible mushroom were collected to complete the macrofungi description in this study. A number of 5 edible wild mushrooms with th eeconomic value reported in this study were: Amanita sect. caesarea (kulat pelandok), Heimioporus sp. (kulat pelawan), Hygrocybe cf. conica (kulat tiong), Phylloporus sp. (kulat sukatan), and Volvariella sp. (kulat sawit). Three species of fungi are ectomycorrhizal forming fungi, namely kulat pelandok with Schima wallichii, kulat pelawan with Tristaniopsis merguensis, and kulat sukatan which host is still unclear. Meanwhile the rest are saprophytic macrofungi. All wild edible mushrooms are members of the phylum Basidiomycota which are divided into 2 orders and 4 families. Heimioporus sp. is known to be the highest selling price mushroom compared to the others, which indicated the potential prospect to proceed to the cultivation stage. In addition, conservation management of plants that are hosts of ectomycorrhizal fungi need to be an important concern on Belitong Island.


Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1972 ◽  
Author(s):  
Zaw Min Thu ◽  
Ko Ko Myo ◽  
Hnin Thanda Aung ◽  
Marco Clericuzio ◽  
Chabaco Armijos ◽  
...  

Mushrooms have a long history of uses for their medicinal and nutritional properties. They have been consumed by people for thousands of years. Edible mushrooms are collected in the wild or cultivated worldwide. Recently, mushroom extracts and their secondary metabolites have acquired considerable attention due to their biological effects, which include antioxidant, antimicrobial, anti-cancer, anti-inflammatory, anti-obesity, and immunomodulatory activities. Thus, in addition to phytochemists, nutritionists and consumers are now deeply interested in the phytochemical constituents of mushrooms, which provide beneficial effects to humans in terms of health promotion and reduction of disease-related risks. In recent years, scientific reports on the nutritional, phytochemical and pharmacological properties of mushroom have been overwhelming. However, the bioactive compounds and biological properties of wild edible mushrooms growing in Southeast Asian countries have been rarely described. In this review, the bioactive compounds isolated from 25 selected wild edible mushrooms growing in Southeast Asia have been reviewed, together with their biological activities. Phytoconstituents with antioxidant and antimicrobial activities have been highlighted. Several evidences indicate that mushrooms are good sources for natural antioxidants and antimicrobial agents


Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 575
Author(s):  
El Hadi Erbiai ◽  
Luís Pinto da Silva ◽  
Rabah Saidi ◽  
Zouhaire Lamrani ◽  
Joaquim C.G. Esteves da Silva ◽  
...  

The present study aimed to investigate the chemical composition, bioactive compounds, and antioxidant activity of two wild edible mushrooms, the honey fungus (Armillaria mellea) and the parasol mushroom (Macrolepiota procera), collected from Northern Morocco (MA) and Portugal (PT). Those species were chosen due to their edibility, nutraceutical, and medicinal properties. Bioactive compounds (ascorbic acid, tannin, total phenolic, total flavonoid, β-carotene, and lycopene) and their antioxidant activity were determined by spectrophotometric methods. Herein, the fruiting body of the samples revealed a significantly higher amount of bioactive compounds, and values varied between the Moroccan and the Portuguese ones. Methanolic extracts shown a strong antioxidant capacity: Using DPPH free radical-scavenging activity radicals (IC50 1.06–1.32 mg/mL); inhibition of β-carotene bleaching radicals (IC50 0.09–0.53 mg/mL); and, reducing power radicals (IC50 0.52–1.11 mg/mL). The mushroom species with the highest antioxidant capacity was A. mellea from MA. Chemical composition was analyzed by GC-MS and LC-MS methodologies. GC-MS analysis showed that the most abundant biomolecules group was sugar compositions in the four samples (62.90%, 48.93%, 59.00%, and 53.71%) and the main components were galactitol 16.74%, petroselinic acid 19.83%, d-galactose 38.43%, and glycerol 24.43% in A. mellea (MA), A. mellea (PT), M. procera (MA), and M. procera (PT), respectively. LC-MS analysis of individual phenolic compounds revealed that vanillic acid (198.40±2.82 µg/g dry weight (dw) and cinnamic acid (155.20 ± 0.97 µg/g dw) were the main compounds detected in A. mellea, while protocatechuic acid (92.52 ± 0.45 and 125.50 ± 0.89 µg/g dw) was predominated in M. procera for MA and PT samples, respectively. In general, the results of this comparative study demonstrate that the geographic and climatic conditions of the collection site can influence biomolecule compounds and antioxidant properties of wild mushrooms. This study contributes to the elaboration of nutritional, nutraceutical, and pharmaceutical databases of the worldwide consumed mushrooms.


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