scholarly journals FID-Gas Chromatography of West-Algerian Cucurbita pepo (or Pumpkin) Seed Oil Extract

2021 ◽  
Vol 2 (3) ◽  
pp. 34-37
Author(s):  
Abdelkrim Berroukche ◽  
Abdelkader Ammam ◽  
Mohamed Terras ◽  
Mohamed Amine Souidi ◽  
Mohamed Chibani ◽  
...  

Fatty acid and phytosterol vegetable oils were extracted from seeds of Cucurbita pepo (or pumpkin) into (v/v) hexane. The extract obtained was characterized by the contents of sterols, unsaturated and saturated fatty acids. The content of the bioactive compounds was determined by gas-chromatography FID method. Pumpkin seed oil extracts showed higher content of poly-unsaturated fatty acids (49.10%) than that from saturated fatty acids (28%). Sterols exhibited different molecules dominated by β-sistostérol (47%), Stigmastérol (23.6%) and campesterol (21.5%). Several peaks were present on the FID GC chromatogram of two extracts (fatty acids and sterols respectively). GC spectra confirmed the presence of the predominant bioactive compounds (PUFA, β-sistostérol , Stigmastérol and campesterol). The composition of pumpkin seed oil extract was characterized by FID-GC spectra with maximum at 280 nm.

2019 ◽  
Vol 11 (2) ◽  
pp. 168-173
Author(s):  
Marijana Blažić ◽  
Elizabeta Kralj ◽  
Marija Agičić ◽  
Irena Perković ◽  
Ivana Kolić

Semi-hard cheese was made from fresh domestic cow milk and treated with pumpkin seed oil and coffee during its ageing. The energy value and composition of the cheese were examined by determining the content of dry matter, minerals, proteins, fat, caffeine and fatty acids. During the ageing period, the cheese was dipped into a sweet, freshly-prepared, high-quality coffee drink and coated with a mixture of milled coffee grains and cold pressed pumpkin seed oil to improve the sensory properties of the cheese, but also to improve its nutritional value. It is considered that treated cheese could have greater nutritional and pro-health properties then untreated cheese, due to nutritional and healing properties of pumpkin seed oil and coffee used during the maturation period. Pumpkin seed oil contains carbohydrates, minerals, proteins, and important unsaturated fatty acids, while coffee is rich in antioxidants and helps in the prevention of type 2 diabetes, Parkinson's and Alzheimer's disease, and high cholesterol. To determine the impact of the treatment, the fatty acid and caffeine contents were determined by the GC-FID and the UPLC-DAD method. The cheese treated with pumpkin seed oil and coffee had a higher amount of unsaturated fatty acids (UFA) and a lower amount of saturated fatty acids (SFA) than the untreated cheese. The proportions of long-chain UFA, such as the C18:2n6c (Omega 6) and the C18:3n3 (Omega 3), were higher in the treated cheese than those in the untreated cheese, as well as the C20:1, the C22:2 and the C24:1, which were not detected in the untreated cheese. Caffeine concentration in the treated cheese was 33.08 mg/L.


2018 ◽  
Vol 46 (2) ◽  
pp. 663-669 ◽  
Author(s):  
Leila REZIG ◽  
Moncef CHOUAIBI ◽  
Rosa Maria OJEDA-AMADOR ◽  
Sergio GOMEZ-ALONSO ◽  
Maria Desamparados SALVADOR ◽  
...  

Pumpkin seed oils are rich in bioactive compounds such as tocopherols, sterols, β-carotene, and lutein that have, along with some fatty acids, high nutritional value factors. In addition, it has so far been proven that these compounds have a positive effect on human health. The present study mainly aimed at evaluating the chemical composition and the bioactive compounds of pumpkin (Cucurbita maxima) seed oil of the ‘Béjaoui’ Tunisian cultivar using both cold pressing and solvent extraction methods. The seed oils contained substantial amounts of unsaturated fatty acids, particularly oleic and linoleic acids, with values ranging respectively from 28.19% for cold pressed pumpkin seed oil to 30.56% for pumpkin seed oil extracted by pentane and from 43.86% for pumpkin seed oil extracted by pentane to 46.67% for cold pressed pumpkin seed oil of the total amount of fatty acids. Investigations of different seed oils revealed that extraction techniques had significant effects on the antioxidant activity and the γ-tocopherol. Cold pressed pumpkin seed oil revealed the highest γ-tocopherol content (599.33 mg kg-1) and the highest oxidative stability (3.84 h). However, the chloroform/methanol extracted pumpkin seed oil, which is rich in total phenolics (54.41 mg Gallic Acid Equivalent kg-1), was a more effective scavenger of DPPH radicals (250 µmoles Trolox kg-1) than the poor phenolic pumpkin seed oils extracted by hexane and pentane (110 µmoles Trolox kg-1 vs 100 µmoles Trolox kg-1). Based on its unique seed oil features, cold press extracted pumpkin seed may add great value to product innovation in the industrial, nutritional, cosmetic, and pharmaceutical fields.


2021 ◽  
Vol 26 (2) ◽  
pp. 2370-2374
Author(s):  
NATALIŢA IONESCU (BORDEI) ◽  
◽  
ANDREEA-MIRUNA NEAGU ◽  
ANCA DANIELA RAICIU ◽  
MARIANA POPESCU ◽  
...  

The aim of the paper was to conduct a study regarding the composition of saturated and unsaturated fatty acids for a series of vegetable oils in order to correlate it with their antioxidant activity. The oils were then used in the development of hydrogels based on nanostructured lipid carriers (NLC) with therapeutic effect on varicose veins. Several vegetable oils (pumpkin seed oil, wheat germ oil, evening primrose seed oil, castor seed oil, grape seed and jojoba oil) were analyzed by gas chromatography coupled with mass spectrometry (GC-MS), establishing the profile of saturated and unsaturated fatty acids. Antioxidant activities were determined by the FRAP (Ferric Reducing Antioxidant Power) method and were correlated with the composition ratio of saturated and unsaturated fatty acids. It has been established that pumpkin seed oil and wheat germ oil are most indicated in the development of NLC-based hydrogels.


Author(s):  
Syamsul RAHMAN ◽  
Salengke Salengke ◽  
Abu Bakar TAWALI ◽  
Meta MAHENDRADATTA

Palado (Aglaia sp) is a plant that grows wild in the forest around Mamuju regency of West Sulawesi, Indonesia. This plant is locally known as palado. Palado seeds (Aglaia sp) can be used as a source of vegetable oil because it contains approximately 14.75 % oil, and it has the potential to be used as food ingredients or as raw material for oil production. The purpose of this study was to determine the chemical properties and the composition of fatty acids contained in palado seed oil (Aglaia sp). The employed method involved the use of palado fruit that had been processed to be palado seed and undergoing flouring process. Palado flour was produced by the extraction process by using chloroform solvent with the soxhlet method. The characteristics of the chemical properties in the oil produced were analyzed by using a standard method, including iodine, saponification, and acid values. The analysis of fatty acid composition was conducted by using gas chromatography. The results showed that palado oil extracted with hexane had an iodine value of 15.38 mg/g, saponification value of 190.01 mg KOH/g, and acids value of 1.961 mg KOH/g. The fatty acid composition of the palado seed oil consisted of saturated fatty acids (41.601 %), which included palmitic acid (41.062 %), myristic acid (0.539 %), and unsaturated fatty acids (45.949 %), which included mono-unsaturated fatty acids (MUFA) such as (22.929 %), oleic acid and poly-unsaturated fatty acids (PUFA), which was linoleic acid (23.020 %).


2020 ◽  
Vol 53 (1) ◽  
pp. 105-120 ◽  
Author(s):  
W. SUN ◽  
M. H. SHAHRAJABIAN ◽  
M. KHOSHKHARAM ◽  
H. SHEN ◽  
Q. CHENG

Cotton (Gossypium L.) is one of the most important commercial crops and it is famous as white gold. Cotton has a diversity of applications, principally medicinal and many other usages, such as pigments, derivatives for cattle feed, different uses of the oil extracts and etc. Cottonseed oil has a ration of 2:1 of polyunsaturated to saturated fatty acids and generally consists of 65-70% unsaturated fatty acids, including 18-24% monounsaturated (oleic) and 42-52% polyunsaturated (linoleic), and 26-35% saturated (palmitic and stearic). The most important health benefits of cotton is treat respiratory diseases, treat skin problems, treat wounds, beneficial for breastfeeding mothers, a good cure for rat bite, an appropriate cure for scorpion bite, for joint and eye pains, for swollen legs, for removing bacteria in teeth, and alternative medicine for various diseases such as cancer, HIV and etc. Cotton seed oil mostly extracted from Gossypium hirsutum and Gossypium herbaceum, that are also grown for cotton fiber and animal feed. Gossypol is one of the most effective ingredients, both in traditional pharmaceutical practices and alternative modern medicinal preparations. It is a toxic polyphenolic bisesquiterpene, which may have antifertility and antiviral properties. The obtained findings suggest potential of cotton as a natural resource in pharmaceutical industries.


2012 ◽  
Vol 260-261 ◽  
pp. 312-317 ◽  
Author(s):  
Ferdinando Corriere ◽  
Giorgia Peri ◽  
Vincenzo La Rocca

Biodiesel from vegetable oils has been analyzed and its suitability for internal combustion engines has been assessed. The Standard biofuel’s characteristics have been investigated. Gas chromatography (GC) tests were carried out to characterize the fatty acid behavior. It was found that the mass percentage of saturated fatty acids did not change substantially with time, while unsaturated fatty acids increased with time for both B100 and B30. An increase in density for B100 and B30 with time was noted. Biodiesel density was within the standardization. The calorific values of biodiesel and its blend increased with time. Higher heating value for B100 increased from 41 to 43 MJ/kg while B30 increased from 44 to 45 MJ/kg.


2016 ◽  
Vol 31 ◽  
pp. 429-436 ◽  
Author(s):  
Betsabé Hernández-Santos ◽  
Jesús Rodríguez-Miranda ◽  
Erasmo Herman-Lara ◽  
Juan G. Torruco-Uco ◽  
Roselis Carmona-García ◽  
...  

Author(s):  
Mohammad Asif ◽  
Guru Ram Das

Perilla frutescens seeds contain saturated fatty acids and unsaturated fatty acids which include the monounsaturated and polyunsaturated fatty acids. These seeds contain approximately 35-40% oil. The oil of Perilla frutescens contains ω-3 fatty acids (54-64%), ω-6 fatty acids (≈14%) and the ω-9 fatty acids (small amount). In comparing to other plant oils, Perilla oil contains one of the highest proportions of ω-3 fatty acids. These polyunsaturated fatty acids are the most beneficial to human health in prevention and control of various diseases like cardiovascular disorders, cancer, inflammation, rheumatoid arthritis etc. This review article describes briefly the benefits and the medicinal uses of Perilla frutescens seeds.


2021 ◽  
Author(s):  
Max Henry Barnhart ◽  
Edward V McAssey ◽  
Emily Dittmar ◽  
John M. Burke

Seed oil composition, an important agronomic trait in cultivated sunflower, varies latitudinally across the native range of its wild progenitor. This pattern is thought to be driven by selection for a higher proportion of saturated fatty acids in southern populations compared to northern populations, likely due to the different temperatures experienced during seed germination. To investigate whether these differences in fatty acid composition between northern and southern populations correspond to transcriptional variation in the expression of genes involved in fatty acid metabolism, we sequenced RNA from developing seeds of sunflowers from Texas, USA and Saskatchewan, Canada (the extreme ends of sunflower's latitudinal range) grown in a common garden. Over 4,000 genes were found to be differentially expressed between Texas and Canada, including several genes involved in lipid metabolism. Many differentially expressed oil metabolism genes colocalized with known oil QTL. The genes producing stearoyl-ACP-desaturases (SAD) were of particular interest because of their known role in the conversion of fully saturated into unsaturated fatty acids. Two SAD genes were more highly expressed in seeds from Canadian populations, consistent with the observation of increased levels of unsaturated fatty acids in seeds from that region. We also constructed a gene co-expression network to investigate regional variation in network modules. The results of this analysis revealed regional differentiation for eight of twelve modules, but no clear relationship with oil biosynthesis. Overall, the differential expression of SAD genes offers a partial explanation for the observed differences in seed oil composition between Texas and Canada, while the expression patterns of other metabolic genes suggest complex regulation of fatty acid production and usage across latitudes.


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