scholarly journals Barangan Banana (Musa acuminata L.) Shoots Induction By Application Of NAA And BAP Based On The Sources Of Explant Basal

2016 ◽  
Vol 1 (2) ◽  
pp. 13
Author(s):  
Hardi Yudha ◽  
Suci Rahayu ◽  
Saleha Hanum
Keyword(s):  
Key Words ◽  
Significant Influence ◽  
Concentration Factor ◽  
Musa Acuminata ◽  
Shoot Induction ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The University ◽  
North Sumatra ◽  
Number Of Shoots

The research or barangan banana (Musa acuminata L.) shoot induction by application of NAA and BAP based on the source of explans basal that had been carried out in the system Culture Laboratory of the University of North Sumatra. This study aims to discover the source of explants forming shoots by application of NAA and BAP and the number of shoots formed by the application of NAA and BAP based sources of banana explants. The study uses a completely randomized design with two factorial, i.e. the concentration factor NAA (0 mg/l, 1.5 mg/l, 2.0 mg/l, 2.5 mg/l, and 3.0 mg/l) and concentration factor BAP (0 mg/l, 4 mg/l, 5 mg/l, 6 mg/l, and 7 mg/l) with the source explants apical and basal parts. The best position that created the shoots explants contained in explant basal part by the number of shoot formed is 83. In the treatment of 1.5 mg/l NAA and 5 mg/l BAP average number of shoots most 3,75 and 2,30 cm tall shoots, the formation of the shoots is in the 80th day. The application of NAA and BAP on basal explants have a significant influence on the formation of the shoots.   Key words: Musa acuminata L., NAA, BAP,explants basal, shoot.

scholarly journals The Potential of Giving Silage Feed Based on Corn Plant Waste (Zea mays) Using Local Microorganisms (Indigenous Microorganism) Against the Percentage of Non-Carcass on Local Sheep

2019 ◽  
Vol 7 (2) ◽  
pp. 65-76
Author(s):  
Bahari Ryansyah Hasibuan ◽  
A H Daulay ◽  
Hasnudi ◽  
M Tafsin ◽  
Yunilas
Keyword(s):  
Significant Influence ◽  
Initial Weight ◽  
Corn Plant ◽  
Indigenous Microorganism ◽  
Randomized Design ◽  
Heart Blood ◽  
Completely Randomized Design ◽  
Four Levels ◽  
The University ◽  
North Sumatra

This study aims to examine the effect of complete corn waste based on silagefeed on the percentage of non carcasses on local sheep. The study was conducted at theLaboratory of Animal Biology at the University of North Sumatra in May - August2018. The design used was a Completely Randomized Design (CRD) with 4 treatmentsand 3 replications using sheep with an average initial weight of 11.2 ± 0.4 kg. Thetreatments consisted of four levels of complete silage feed based on corn waste P1(20%), P2 (40%), P3 (60%), and P4 (80%).The parameters observed were: weight,head, foot, skin, tail, trachea and lungs, liver, heart, blood, and digestive tract.Theresults showed that complete feed based corn waste silage did not have a significanteffect (P> 0.05) on the percentage of non-carcass weight such as skin, tail, trachea andlungs, blood and digestive tract, but gave a non-significant effect on the percentage noncarcass weights such as head, feet, heart and liver. The conclusion of complete feedsilage based on corn waste can have a significant influence on the internal non carcass(liver and heart) and external (head and foot) parts of male local sheep. In addition itgives a non significant influence on the non-internal carcass parts (trachea and lungs,blood and digestion) and the external (skin and tail) of male local sheep.

scholarly journals Effects of Organic-based Fortified Foliar Fertilizer on the Growth and Yield of ‘Lakatan’ Banana (Musa acuminata)

2019 ◽  
pp. 1-9
Author(s):  
Alminda Magbalot-Fernandez ◽  
Lara Montifalcon
Keyword(s):  
Fruit Weight ◽  
Musa Acuminata ◽  
Growth And Yield ◽  
Average Yield ◽  
Foliar Fertilizer ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Npk Fertilizer ◽  
Completely Randomized Design ◽  
The University

To determine the effect of Organic-based Fortified Foliar Fertilizer (OFF) on the growth and yield of ‘Lakatan’ banana, this study was conducted at the University of Southeastern Philippines, Tagum – Mabini Campus, Mabini Unit, Mampising, Mabini, Compostela Valley Province, from December 2015 to March 2016.          The experiment was laid out in Completely Randomized Design (CRD) with six treatments replicated three times. The treatments were: T1- control; T2- Recommended Rate of NPK fertilizer/ha; T3 - ½ RR of NPK/ha; T4 –Organic-based Fortified Foliar fertilizer (OFF) at 100 ml/16 liters of water; T5 - ½ RR of NPK + OFF; and T6 - RR of NPK + OFF. Results showed that no significant effects were observed in terms of the plant height at 15, 30, 45 days after application (DAA), pseudostem girth at 15, 30, 45 DAA, and number of hands per bunch. However, significant differences were obtained in the number of leaves, number of fingers per bunch, fruit weight (kg) and yield (tons/ha) of ‘Lakatan’ banana. The highest number of leaves were obtained in T6 – RR of NPK fertilizer/ha + OFF which is comparable to T5 – ½ RR of NPK fertilizer/ha + OFF. T6 – RR of NPK+ OFF also had the highest number of fingers per hand than the rest of the treatments as much as 21% higher than the control. The fruit weight of T6 – RR of NPK+ OFF has 61% higher than untreated and the yield of 37 tons/ha is higher than the national average yield of 9.4 tons/ha in ‘Lakatan’ banana.

scholarly journals Induction of Barangan Banana shoot (Musa acuminata L.) from North Nias through Tissue Culture by giving 2,4-D and Kinetin

2016 ◽  
Vol 1 (2) ◽  
pp. 1
Author(s):  
Destarius Zebua ◽  
Suci Rahayu ◽  
Saleha Hanum
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Musa Acuminata ◽  
Basal Part ◽  
Banana Weevil ◽  
Shoot Induction ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Basal Position

The research on induction of banana barangan shoot (Musa acuminata L.) from North Nias through tissue culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue Culture  University of North Sumatera from May until October 2014. The main objective of this research was to know the explants of banana weevil in apical and basal position with growth regulator 2,4-D with kinetin which are able to induce shoots from callus initials. On this research, the treatment tested for induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1 mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was designed using Completely Randomized Design (CRD) two factorial with repetition of experiment. The results of research showed that the interaction between concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days) forming the shoot derived from explant basal part. The concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for 3.00 and average number of highest shoots for 1.50 cm. Keywords: Musa acuminata, weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin.

scholarly journals Pengaruh Jumlah Ruas Cabang Terhadap Pertumbuhan Setek Bambu Hitam (Gigantochloa Atroviolacea)

2014 ◽  
Vol 2 (1) ◽  
pp. 59
Author(s):  
Wenty Irvantia ◽  
Indriyanto . ◽  
Melya Riniarti
Keyword(s):  
Key Words ◽  
Bamboo Species ◽  
Randomized Design ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Shoots

Black bamboo (Gigantochloa atroviolacea) is one of the famous bamboo species used, because it has many functions.The effort to maintain bamboo population is by cultivation. Generative cultivation of bamboo is difficult, so that vegetative cultivation by branch cuttings is carried. The aim of this research were to know the effect of number of internodes on black bamboobranchcuttings and the best influence on percentage of life and growth.The experiment used Completely Randomized Design(CRD) with 3treatmentsand 5replications.The treatmentwerenumber ofblack bamboobranch, which were2, 3,and 4 internodes. Eachbranchesplantedin polybag had filledwith top soil.The growth variablesthat measured were the percentage of life, number of shoots, length of shoot, diameter of shoot, number of leaves andlength of root. Theresults showed that thenumber of black bamboo branchinternodesinfluenced onlength of shoot and number of leaves. Blackbamboobranch with 4 internodeswas thebestresultto growth ofblackbamboobranchcuttings because of highestvalue onlength of shoot (3.84 cm) and number of leaves(4.90 sheet). Key words: black bamboo, branch cuttings, number of internodes

scholarly journals INDUKSI TUNAS TABAT BARITO (Ficus deltoidea JACK) SECARA IN VITRO MENGGUNAKAN BENZIL ADENIN (BA) DAN NAPHTHALENE ACETIC ACID (NAA)

2020 ◽  
Vol 15 (1) ◽  
pp. 33
Author(s):  
NATALINI NOVA KRISTINA
Keyword(s):  
Second Step ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
High Concentration ◽  
Shoot Height ◽  
Ficus Deltoidea ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Number Of Shoots

<p>ABSTRAK<br />Tabat barito (Ficus deltoidea Jack), merupakan salah satu tanaman<br />obat yang dikategorikan langka dan digunakan sebagai bahan afrodisiak<br />wanita. Perbanyakan tanaman secara in vitro dilakukan untuk mendapatkan<br />bahan tanaman dalam jumlah banyak. Penelitian bertujuan untuk<br />mendapatkan media terbaik tabat barito dan telah dilakukan di laboratorium<br />kultur jaringan Plasma Nutfah dan Pemuliaan Balittro, pada bulan<br />Januari sampai dengan Desember 2007. Penelitian ini dilakukan dalam tiga<br />tahap, yaitu : 1) respon tunas pada media perbanyakan, menguji media<br />multiplikasi tunas dengan media sitokinin tunggal yaitu : MS + Benzil<br />Adenin (BA) 0,5; 1,0; 1,5 dan 2,0 mg/l; tahap 2) respon tunas pada media<br />kombinasi sitokinin dan auksin, yaitu : MS + BA 0,5 mg/l + NAA 0,1<br />mg/l; MS + BA 0,5 mg/l + NAA 0,5 mg/l; MS + BA 1,0 mg/l + NAA 0,1<br />mg/l dan MS + BA 1,0 mg/l + NAA 0,5 mg/l. Tahap 3) Daya multiplikasi<br />dan penampilan tunas setelah subkultur pada media yang sama. Masingmasing<br />percobaan disusun dengan rancangan acak lengkap, dan terdiri atas<br />5 ulangan. Parameter pengamatan meliputi jumlah tunas, tinggi tunas dan<br />jumlah ruas serta penampilan visual. Hasil penelitian menunjukkan bahwa<br />pada tahap pertama, penggunaan media MS + BA 0,5 menghasilkan<br />jumlah tunas yang paling banyak dibandingkan yang lain, tetapi tidak<br />berbeda nyata pada jumlah ruas dan tinggi eksplan. Sementara pada tahap<br />dua, jumlah tunas terbaik didapat pada media dengan auksin rendah baik<br />pada kombinasi sitokinin rendah dan tinggi. Sementara untuk jumlah ruas<br />media terbaik adalah media dengan konsentrasi BA tinggi yang<br />dikombinasi dengan NAA. Untuk tinggi tunas, media terbaik adalah MS +<br />BA 1,0 mg/l + NAA 0,5 mg/l, tapi ditemukan eksplan yang menguning.<br />Pada tahap ketiga, dari hasil subkultur kembali terlihat bahwa tunas yang<br />bersumber dari pucuk pertumbuhannya baik sementara tunas yang berasal<br />dari ruas ke-2 dan 3 sebagian menguning.<br />Kata kunci : Ficus deltoidea Jack, tunas, induksi, in vitro, BA, NAA</p><p>ABSTRACT<br />In vitro shoot induction of Mistleteo fig (Ficus deltoidea<br />Jack) in Murashige &amp; Skoog (MS) media with addition of<br />BA and NAA<br />Mistleteo fig (Ficus deltoidea) is one of endangered medicinal<br />plants and used for female aphrodisiac. In vitro multiplication of the plant<br />was done to find a number of shoots. This experiment was conducted in<br />tissue culture laboratory of Germplasm and Breeding of IMACRI from<br />January to December 2007, and aimed to find best media for shoot<br />multiplication. This experiment was carried out in three steps: step 1)<br />shoot respon in multiplication media using single cytokinin : MS + BA<br />(0.5; 1.0; 1.5 and 2 mg/l); step 2) shoot respon in multiplication media of<br />combined cytokinin and auxin : MS + BA 0.5 mg/l + NAA 0.1mg/l; MS<br />+ BA 0.5 mg/l + NAA 0.5 mg/l; MS + BA 1.0 mg/l + NAA 0.1 mg/l and<br />MS + BA 1.0 + NAA 0.5 mg/l; and step 3) viability and visualization of<br />the shoots after subcultured in the same media. The experiment was<br />arranged using completely randomized design with 5 replicates. The<br />parameters observed were of shoots and nodes, shoot height and<br />performance. The results in the first step showed that MS + BA 0.5 mg/l<br />media resulted in the highest number of shoots, but they were not<br />significantly different in the number of nodes and shoots height. In the<br />second step, highest number of shoots was found using low concentration<br />of auxin combined with low and high concentration of cytokinin. Best<br />medium for number of nodes was MS with high concentration of BA<br />combined with NAA. For shoot height, the best medium was MS + BA<br />0.1 mg/l + NAA 0.5 mg/l, but the shoots turned yellow. In the third step,<br />after subcultured, the shoots originated from plant tips performed well,<br />however, those taken from second and third inter nodes partially turned<br />yellow.<br />Key words : Ficus deltoidea Jack, shoot, induction, in vitro, BA, NAA</p>

INVESTIGATIONS ON FORAGE YIELD OF OAT GENOTYTPES UNDER IRRIGATED CONDITIONS OF KONYA

2021 ◽  
Vol 9 (2) ◽  
pp. 85-88
Author(s):  
Muhammad Ahsan Qureshi ◽  
Waqar Shafqat
Keyword(s):  
Mangifera Indica ◽  
Forage Yield ◽  
Root Induction ◽  
Shoot Induction ◽  
Tropical Fruits ◽  
Asexual Propagation ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
High Concentrations ◽  
Direct Shooting

Shoot tips of newly emerging mango shoots were used as an explant and cultured on MS media for direct shooting. Different plant growth regulators i.e. BAP, NAA and IAA in varying concentrations were added to basal MS media. Experiment was laid out according to Completely Randomized Design (CRD) with four treatments and each treatment was replicated thrice. Maximum shoot induction (55.56%) was observed with 1 mg/L of BAP. Maximum mortality (83.30%) was observed when maximum concentration of BAP (3 mg/L) was used. Minimum days (12.633) to induce shoots were observed on MS media supplemented with 1 mg/L of BAP. Maximum number of shoots (2.50) were recorded in MS media supplemented with 1 mg/L of BAP. Low concentration of NAA initiated roots earlier in regenerated shoots as compare to high concentrations of NAA and IAA. MS media supplemented with NAA (1 mg/L) took less days (21 days) to induce roots. The combination of auxins (MS+ NAA 3 mg /L + IAA 1 mg /L) proved the best for root induction (38.87%). Keywords: Mangifera indica, tropical fruits, asexual propagation, plant tissue culture, micropropagation, phenolic exudation, acclimatization.

scholarly journals PENGARUH JUMLAH MATA TUNAS BATANG ATAS DAN TINGGI BATANG BAWAH PADA SAMBUNG PUCUK TERHADAP PERSENTASE TUMBUH JAMBU AIR (Syzygium Samarangense)

2020 ◽  
Vol 7 (2) ◽  
pp. 99
Author(s):  
Supriyono Supriyono ◽  
Tarwa Mustopa ◽  
Nunuk Helilusiatiningsih ◽  
Fristama Maulana
Keyword(s):  
Significant Interaction ◽  
Treatment Combination ◽  
Design Factor ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Shoots

Water guava (Syzygium Samarangense) was a popular plant in Indonesia because it was fresh and sweet. The aim of this research was to analyze the effect of the number of shoots of the upper stems and height of the rootstock at the shoot graft on the percentage of growing water guava. The method used in this study was a completely randomized design, factor into one rootstock length, namely 10 cm, 15 cm, and 20 cm. and the second factor was the number of buds on the scion, namely 2, 3, and 4. The results showed that there was a significant interaction in the treatment combination of the number of 4 buds on the upper stem and the length of the rootstock 15 cm at 35 days after grafting affected the growth percentage and growth in the number of leaves and length of the scion.

scholarly journals Motilitas dan viabilitas spermatozoa semen sexing kambing peranakan etawa (pe) menggunakan metode sedimentasi putih telur dengan pengencer yang berbeda

2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Significant Influence ◽  
Research Method ◽  
Egg Yolk ◽  
Egg White ◽  
Variance Analysis ◽  
Laboratory Research ◽  
Sedimentation Method ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.

scholarly journals Comparison between Single and Double Injection of Pituitary Gland (PG) on the Breeding Performance of Climbing Perch, Anabas Testudineus (Bloch)

1970 ◽  
Vol 14 ◽  
pp. 57-60
Author(s):  
Shahid Uddin Mahmood
Keyword(s):  
Pituitary Gland ◽  
Key Words ◽  
Single Injection ◽  
Breeding Performance ◽  
Female Fish ◽  
Anabas Testudineus ◽  
Double Injection ◽  
Climbing Perch ◽  
Randomized Design ◽  
Completely Randomized Design

An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. Key words: Breeding, pituitary gland (PG), fertilization, hatching J. bio-sci. 14: 57-60, 2006

scholarly journals The Insecticide Toxicity and Repelling Ability of Jeruk Purut (Citrus hystrix D.C) Leaf Extract on Maize Weevil (Sitophilus zeamais Motsch)

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Alfia Wulansari ◽  
Nur Rochman ◽  
Setyono Setyono
Keyword(s):  
Mortality Rate ◽  
Key Words ◽  
Leaf Extract ◽  
Insect Pest ◽  
Sitophilus Zeamais ◽  
Maize Weevil ◽  
Insecticide Toxicity ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sitophilus Zeamais Motsch

Sitophilus zeamais Motsch. is a primary storage insect pest damaging maize grains. Control of S. zeamais can be done by applying biopesticide. This study was aimed at assessing the ability of Jeruk Purut leaf extract, as biopesticide, to kill and repel Sitophilus zeamais Motsch. The study was conducted at Entomology Laboratory, Seameo Biotrop in March to August 2018. A completely randomized design with three replicates in each rate of Jeruk Purut leaf extract was used. In preliminary pesticide toxicity experiment, five rates of Jeruk Purut leaf extract, namely 0%, 2%, 4%, 6%, and 8% were applied. In preliminary repelling ability experiment, five rates of Jeruk Purut leaf extract, namely 1%, 2%, 4%, 6%, and 8% were applied. In the main pesticide toxicity and repelling ability trial, five rates of Jeruk Purut leaf extract, namely 3%, 6%, 9%, 12%, and 15% were applied. Results showed that in 24 hour after treating (HAT) no significant effects were found. Significant effects of Jeruk Purut leaf extract as insecticide on S. zeamais were found in 48 and 72 HAT. The highest mortality rate (82.2%) of S. zeamais was found in 15% rate of Jeruk Purut leaf extract in 72 HAT. The application of Jeruk Purut leaf extract was not found to give significant effects on repelling S. zeamais in 24, 48, and 72 HAT as indicated in their sharply fluctuating repellent values. The highest repelling ability (95%) was found in the application of 9% Jeruk Purut leaf extract in 24 HAT. It was concluded that Jeruk Purut leaf extract was more potential as insecticide repellent for Sitophilus zeamais Motsch. Key words: biopesticide, repellent, insecticide, maize, primary storage insect pest

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