scholarly journals Total Phenolic Content, Antioxidant and Antibacterial Activities of Sargassum polycystum of Ethanol Extract from Waters of Kabung Island

2021 ◽  
Vol 9 (3) ◽  
pp. 139
Author(s):  
Ikha Safitri ◽  
W. Warsidah ◽  
Mega Sari Juane Sofiana ◽  
Arie A. Kushadiwijayanto ◽  
T. Novi Sumarni
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Antioxidant Vitamin ◽  
Dpph Method

Sargassum policystum is one of the genus Sargassum from the brown macroalgae group which has an abundance of chemical constituents of fucoidan, fucoxanthin, phenolic acids and alginate which have important biological activities, such as antioxidants and antibacterials. This species thrives in the waters of Kabung Island, Bengkayang, West Kalimantan and has not been exploited so that it tends to act as a marine weed. This study aimed to determine the total phenolic content of the ethanolic extract of S. policystum and its antioxidant and antibacterial activity. Determination of the total content of phenolic compounds using the Folin Ciocalteu method, antioxidant testing using the DPPH method (2,2-diphenyl-1-picrylhydrazyl) and the indicator of sample color change with the addition of these radicals was measured by UV-Vis spectrophotometer. Antibacterial testing was carried out by the test sample diffusion method and the inhibition of the growth of the test bacteria Staphylococcus aureus and Escherichia coli as test bacteria was indicated by the formation of a clear area around the sample wells. The results showed that the phenol content of the ethanolic extract of S. policystum was 79.8 mg/g extract, its antioxidant activity (IC50) was 98.903 ppm, classified as a strong antioxidant, while the comparison antioxidant, Vitamin C, had an IC50 of 6.26 ppm. The inhibitory ability of the ethanol extract against the test bacteria used was indicated by the formation of the largest inhibition diameter on S. aureus of 12.4 mm and E. coli of 12.0 mm.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

Chemical components and biological activities of two freshwater green algae from Ordu, Turkey / Türkiye’nin Ordu ilinden iki tatlı su yeşil alglerinin kimyasal bileşenleri ve biyolojik aktiviteleri

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Beyhan Taş ◽  
Ömer Ertürk ◽  
Özlem Yılmaz ◽  
Melek Çol Ayvaz ◽  
Emine Yurdakul Ertürk
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Algal Species ◽  
Acetone Extract ◽  
Diethyl Ester ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Green Algal

AbstractObjective: Scientists are looking for new resources which have biological activities. The present study was planned to evaluate the antioxidant and antimicrobial activity of ethanol and acetone extracts, as well as the volatile compounds of two freshwater green algal species Spirogyra spp. and Zygnema stellinum (Vaucher) C. Agardh belonging to Zygnemaphyceae (Conjugatophyceae) obtained from Ordu University Campus wetlands.Methods: The extracts were tested in vitro for their antimicrobial effects using disc diffusion method. Total phenolic content and the antioxidative activity according to FRAP and DPPH methods of the extracts were also determined. The secondary metabolites from the investigated extracts were identified using GC-MS.Results: The extracts dramatically inhibited almost all tested microorganisms. The antimicrobial activity of the acetone extract of the Zygnema against C. albicans was found as more higher than positive control Nystatin. In accordance with antimicrobial activity, the highest total phenolic content was also determined in the presence of the acetone extract of the Zygnema. Furthermore the highest FRAP value and the lowest EC50 (mg/mL) value were calculated for the same extract. The main components of the all consisted of dimethyl and diethyl ester of 1,2-benzenedicarboxylic acid. However, the abundance of these metabolites in the extracts was not associated with antimicrobial or antioxidant activity. Biological activities of these algal species could be attributed to chemicals such as 1-Pentadecene and 1-Tetradecene which were present in smaller amounts.Conclusion: Investigated algal species can be evaluated to use in biotechnological applications such as food industry and medicine.

scholarly journals The antimicrobial activity of Prunella vulgaris extracts

2015 ◽  
Vol 61 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Atefeh Mahboubi ◽  
Nastaran Kazempour
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Prunella Vulgaris ◽  
Ethanol Extracts

Summary Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

scholarly journals KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE

Biomedika ◽  
2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Ika Trisharyanti Dian Kusumowati ◽  
Rosita Melannisa ◽  
Kartikaning Ratri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Positive Correlation

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds

scholarly journals COMPARATIVE STUDIES OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF IN VIVO PLANT AND IN VITRO CALLUS EXTRACT OF CARDIOSPERMUM HALICACABUM L.

2021 ◽  
pp. 94-103
Author(s):  
THANIARASU R ◽  
LOGESHWARI M
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Biologically Active ◽  
Diffusion Method ◽  
Total Phenolic

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

Chemical characterization of ethanolic extract of Butterfly pea flower (Clitoria ternatea)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 127-134
Author(s):  
T.N.M. Tuan Putra ◽  
M.K. Zainol ◽  
N.S. MohdIsa ◽  
N. MohdMaidin
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Light Exposure ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Clitoria Ternatea ◽  
Dpph Method ◽  
Butterfly Pea

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

scholarly journals The Chemical Composition, Antioxidant and Anti-Tyrosinase Activities, and Irritation Properties of Sripanya Punica granatum Peel Extract

Cosmetics ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 7 ◽  
Author(s):  
Nachtharinee Laosirisathian ◽  
Chalermpong Saenjum ◽  
Jakkapan Sirithunyalug ◽  
Sukum Eitssayeam ◽  
Busaban Sirithunyalug ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Chorioallantoic Membrane ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Peel Extract

This is the first study to investigate the effect of ethanol concentrations on the phenolic content and biological activities of Sripanya Punica granatum peel extract. Various concentrations of ethanol, (20 to 80% v/v), were used in the maceration process. Extracts were investigated for total phenolic content by the Folin–Ciocalteu method, antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and anti-tyrosinase activity by a spectrophotometric method. The chemical composition was investigated using high-performance liquid chromatography. The irritation of the extracts was determined by Hen’s egg test–chorioallantoic membrane assay. The results demonstrated that E60, which was extracted using 60% v/v ethanol, had the highest yield (66.23 ± 0.38% w/w), the highest total phenolic content (490.42 ± 7.20 μg GAE/g), and the highest antioxidant and anti-tyrosinase activities. IC50 values on DPPH• and ABTS•+ inhibition were 10.97 ± 0.97 and 6.48 ± 0.06 µg/mL, respectively. The 60% v/v ethanol extract (E60) possessed strong tyrosinase inhibition with an IC50 value of 0.10 ± 0.13 µg/mL, which is more potent than kojic acid. The major constituents were punicalagin (4.05 ± 0.26% w/w) and ellagic acid (0.63 ± 0.04% w/w). E60 was safe for topical application since it did not induce irritation. Therefore, we suggest that E60 is a promising agent for further product development.

scholarly journals Korelasi Kajian Fisikokimia Ekstrak Klika Faloak (Sterculia populifolia DC.) Menggunakan Variasi Pelarut Terhadap Penghambatan Bakteri Patogen

2018 ◽  
Vol 4 (1) ◽  
pp. 12-17
Author(s):  
Reny Syahruni ◽  
Syamsu Nur ◽  
Akhmad Amrullah ◽  
Novianti Tonapa ◽  
Vivi Shelina
Keyword(s):  
Inhibitory Activity ◽  
Total Phenolic Content ◽  
Pathogenic Bacteria ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Solvent Polarity ◽  
Acetone Extract ◽  
Diffusion Method ◽  
Bark Extract ◽  
Total Phenolic

Faloak (Sterculia populifolia DC.) is one of species of sterculiaceae found in East Nusa Tenggara which has potential as a medicinal plant mainly as an antimicrobial. This study aims to determine the correlation of physicochemical study of faloak bark extracts with variation of solvents in inhibiting of pathogenic bacteria. The sample was extracted by maceration method with different polarity level of solvents i.e acetone, acetone 70%, water, ethanol 96%, ethanol 70% and ethanol 50%. The results of extraction through maceration indicate the difference of yield recovery from each of the extraction solvents. The highest yield was obtained from 70% ethanol extract, while the lowest yield of acetone extract. The increase of solvent polarity in this study did not give effect to the amount of recovery of yield. It is also seen from the highest total phenolic content obtained from 70% acetone extract while the lowest in aquadest extract. The antibacterial activity of faloak bark extract on Salmonella typhi was tested using agar diffusion method with 1% of extract solution. Both of ethanol 96% and acetone extracts did not show any inhibitory activity. The largest inhibitory activity was demonstrated by 50% ethanol extract. The polarity level of the extract, the level of total phenolic content and the magnitude of rendement  did not show correlation of increased inhibitory activity on Salmonella thypi as well.

scholarly journals Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to Superoxide Dismutase (SOD) Level in Mice

2018 ◽  
Vol 8 (3) ◽  
pp. 101
Author(s):  
Hendri Asrin ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Marianne Marianne
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
Total Phenolic Content ◽  
Staining Method ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Control Group ◽  
Dpph Method

A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi

scholarly journals Ethnobotanical, Phytochemical, and Biological Study of Tamarix aphylla and Aerva javanica Medicinal Plants Growing in the Asir Region, Saudi Arabia

2019 ◽  
Vol 12 ◽  
pp. 194008291986948
Author(s):  
Mohamed Hammad Adam Suleiman
Keyword(s):  
Saudi Arabia ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Biological Study ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Medicinal Uses ◽  
Phytochemical Constituents ◽  
Asir Region

In this study, Tamarix aphylla and Aerva javanica, plants used in folkloric medicine in the Asir region of Saudi Arabia were studied ethnobotanically, chemically, and biologically to assess their medicinal uses, phytochemical constituents, and biological activities. Ethnobotanical data were collected using semistructured interviews and the use values were calculated. A total of 61 informants were interviewed and results indicated high-value uses (0.9 and 0.7) for the plants. Phytochemical investigation indicated that the plants contained most of the phytochemicals that were tested for. The antioxidant activity was examined by assaying total phenolic content, 1,1-Diphenyl-2-picrylhydrazyl (DPPH), and hydrogen peroxide (H2O2) radical scavenging activity. .The bark extract of T. aphylla showed the best antioxidant effect in total phenolic content, DPPH, and H2O2 assays (278.02 ± 0.16 mg GAE/100 g; The half-maximal inhibitory concentration (IC50): DPPH, 18.39 ± 0.62 μg/mL; H2O2, 252.94 ± 1.86 μg/mL), followed by extract of the A. javanica aerial parts (228.60 ± 2.09 mg GAE/100 g; IC50: DPPH, 28.54 ± 0.53 μg/mL; H2O2, 154.17 ± 0.78 μg/mL) compared with ascorbic acid (DPPH: 27.27 ± 0.11 μg/mL; H2O2: 164.9 ± 0.37 μg/mL). The disc diffusion method performed for antimicrobial activity revealed weak activities in T. aphylla and A. javanica extracts (100 μg/mL) against six human pathogens. It was concluded that these plants possess therapeutic potential in the treatment of 18 types of ailments, which are important phytochemical constituents and antioxidant activities that justify their therapeutic uses in traditional medicine. Thus, this study laid sufficient background for further pharmacological research on extracts of these plants.

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