scholarly journals Gas chromatography-mass- spectroscopy analysis of bioactive compounds from Streptomyces spp. isolated from Tigris river sediments in Baghdad city

2020 ◽  
Vol 14 (1) ◽  
pp. 63-71
Author(s):  
Talib S. Al-rubaye ◽  
Mohsen H. Risan ◽  
Dalal Al-rubaye
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Mass Spectroscopy ◽  
River Sediments ◽  
Antibacterial Activities ◽  
Hexanoic Acid ◽  
Molecular Formula ◽  
Streptomyces Spp ◽  
Secondary Screening ◽  
Amine Compounds

Background:The Streptomyces are considered the most important bacterial source for bioactive compounds production including natural antibiotics. Objective: This study focused on analysis of these products to characterize the most active substances which may contain new antibiotics. Materials and methods: Samples with the highest antibacterial activities (21, M5, N- and D-) were chosen from a previous study after secondary screening for the intracellular (biomass) extract which showed more antagonism efficiency than that observed in extracellular crude extract.Gas chromatography – mass spectroscopy (GC-MS) was preformed to detect the structure of the compounds in intracellular crude extracts in these isolates. Results: The GC-MS analysis showed a total of 49 peaks observed in 4 isolates, isolate M5=14 peaks, isolate D=11peaks, isolate N= 20 peaks and isolate 21= 4peaks. Isolate D-, which showed the highest zone of inhibition in secondary screening than that in other isolates, is associated with the most prevalence active compounds like the Decane derivatives, in addition to Triadimenol; Azetidine, 1-(1,1-dimethylethyl)-3-methyl; Hexanoic acid, 2-ethyl-, 2-ethylhexyl ester and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane. While isolate 21, has less peaks in comparing with the other samples, with great occurrence in components: 1-Dimethylaminohexane with molecular formula C8H19N and molecular weight 129 and Propamocarb with molecular formula C9H20N2O2 and molecular weight 188, in addition to many volatile organic compounds. The greatest components of isolate M5 were Triadimenol and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane, in addition to the presence of Decane derivatives; amine compounds and Vitamin E. Isolate N- showed a great occurrence with components Triadimenol and Azetidine, 1-(1,1-dimethylethyl)-3-methyl-with a molecular formula C8H17N and with a molecular weight 127; also the presence of an important component Hexanoic acid, 2-methyl- with the molecular formula C7H14O2 and with molecular weight 103 which has been considered as an essential component of muramycin antibiotic; compounds which contain  Benzene ring. Conclusion: The most prominent compounds detected in the selected isolates by using GC-MS technique were Decane derivatives and Triadimenol.

scholarly journals Phytochemical Profiling of Leaf, Stem, and Tuber Parts of Solena amplexicaulis (Lam.) Gandhi Using GC-MS

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Karthika Krishnamoorthy ◽  
Paulsamy Subramaniam
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Plant Extracts ◽  
Mass Spectra ◽  
Molecular Formula ◽  
Good Source ◽  
Methanolic Extracts ◽  
Solena Amplexicaulis ◽  
Structure Category ◽  
Molecular Weight Peak

Objective. To explore the possible bioactive compounds in the methanolic extracts of leaf, stem, and tuber parts of the medicinal climber, Solena amplexicaulis, using GC-MS. Methods. GC-MS analysis of the plant extracts were performed by using GC-MS-5975C [Agilent] and mass spectra of the compounds found in the extract was matched with the data in the library of National Institute of Standards and Technology (NIST). Results. Thirty-five compounds were determined to be present in the parts studied. The active principles with their retention time, molecular formula, molecular weight, peak area, structure, category of the compounds, and activities were predicted. The most prevailing compounds were phytol (38.24%) in leaf, 4-(4-ethoxyphenyl) but-3-en-2-one (56.90%) in stem, and 9,17-octadecadienal, (Z)- (21.77%) in tuber. Conclusion. This study revealed that the species S. amplexicaulis is a good source of many bioactive compounds like terpenes, triazines, esters, alkanes, alcohols, hydrocarbons, aldehydes, amides, and so forth. That justifies the traditional usage of this species.

scholarly journals GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN ETHANOL LEAVES EXTRACT OF SPHENOCENTRUM JOLLYANUM AND THEIR BIOLOGICAL ACTIVITIES.

2022 ◽  
Vol 06 (01) ◽  
Author(s):  
Emmanuel Uka1,
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Molecular Formula ◽  
Hexadecanoic Acid ◽  
Total Extract ◽  
Medicinal Uses ◽  
Ms Analysis

Sphenocentrum jollyanum is a plant genus of the family Menispermaceae. It has high medicinal importance as it is used traditionally to treat various diseases such as jaundice, breast engorgement related to the menstrual cycle, tumour, fibroids and improve the health of people. The present investigation was carried out to analyze the bioactive compounds present in ethanol crude extract of Sphenocentrum jollyanum leaves using GC-MS analysis. GC-MS analysis of ethanol extract Sphenocentrum jollyanum was done using a 7890A GC system (Agilent Technologies), coupled with 5977B MSD (Agilent Technologies) while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. A total of 45 bioactive compounds representing 99.98% of the total extract based on the retention time, peak area, molecular formula, molecular weight, and biological activities were identified by GC-MS which ranges from high molecular weight to low molecular weight compounds. The major compounds identified with their peak area percentages were 2,4-Di-tertbutylphenol, (21.05%), Z-8-Methyl-9-tetradecenoic acid (19.12), Hexadecanoic acid, ethyl ester (7.86%), Diisooctyl phthalate (7.13%), Phytol, Oleic Acid (7.03), 6,9,12- Octadecatrien-1-ol (6.65%), 3-Eicosene, (E)-(4.63%), 2-Methyl-Z, Z-3,13-octadecadienol (4.24%), n- Hexadecanoic acid (4.09%), trans-13-Octadecenoic acid (3.81%), Cyclohexene, 4-(4-ethylcyclohexyl) -1- pentyl- (3.74%), Dibutyl phthalate (3.20%), and 9-Oxabicyclo (6.1.0) nonane, cis-(3.18%). The presence of these major phytoconstituents in the leaf extract provides various biological activities including antifungal, antibacterial, antioxidant, anti-inflammatory, and anti-tumour which supports the ethno-medicinal uses of the plant in curing diseases. We recommend

Analysis of Bioactive Compounds of Methanolic Leaves extract of Mentha pulegium Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique

2017 ◽  
Vol 8 (04) ◽  
Author(s):  
Israa Adnan Ibraheam ◽  
Mohammed Yahya Hadi ◽  
Imad Hadi Hameed
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Mentha Pulegium

The objective of this study was analysis of the secondary metabolite products. Bioactives are chemical compounds often referred to as secondary metabolites. Sixteenth bioactive compounds were identified in the methanolic extract of Mentha pulegium. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Mentha pulegium revealed the existence of the Erythritol , Cyclohexanone , 3-methyl-,(R)- , 2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one , 1-Oxaspiro[2.5]octan-4-one ,2,2,6-trimethyl-, cis- , Terpinyl formate , Acetamide , N-methyl-N-[4-(3-hydroxypyrrolidinyl)-2-butynyl]- , Pulegone , 2-Oxabicyclo[3.3.0]oct-7-en-3-one , 7-(1-hydroxypentyl)- , 2(3H)-Naphthalenone ,4,4a,5,6,7,8-hexahydro-1-methoxy- , 2-Cyclopenten-1-one , 2-(2-butenyl)-4-hydroxy-3-methyl-,(Z)- , (5β)Pregnane-3,20β-diol 14α,18α-[4-methyl-3-oxo-(1-oxa-4- , 2-(4-(But-2-yl)phenyl) propnoic acid , Nootkaton-11,12-epoxide , 2-Heptanone , 6-methyl-6-[3-methyl-3-(1-methylethenyl)-1-cyclo , Cholestan-3-ol , 2-methylene-, (3β,5α)- , 1-Heptatriacotanol and Digitoxin.

scholarly journals Cold Steeping Infusion, a Novel Lectin Extraction Technique for the Isolation, Purification and Partial Characterization of Lectins from the Green Venezuelan Marine Alga Caulerpa serrulata

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Pablo Djabayan-Djibeyan ◽  
Brian Carpenter ◽  
Gerardo Medina-Ramírez ◽  
Felix Andueza-Leal ◽  
Andrés León-Leal ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Red Blood Cells ◽  
Ammonium Sulfate ◽  
Bioactive Compounds ◽  
Blood Cells ◽  
Marine Alga ◽  
Continuous Production ◽  
Isotonic Saline ◽  
Size Exclusion ◽  
Extraction Technique

A lectin from the green Venezuelan marine alga Caulerpa serrulata was extracted with phosphate buffered saline (PBS) using cold steeping infusion (CSI) and by grinding with liquid nitrogen (GLN). The proteins were precipitated using solid ammonium sulfate. Both the crude extracts and ammonium sulfate precipitated proteins were tested for hemagglutinins using native and papain-treated human red blood cell suspensions in isotonic saline solution. Purification of lectins was achieved using affinity chromatography-sugar-epoxy-sepharose 6B and molecular weight was assessed by size exclusion chromatography using Bio-gel® P-100 and SDS-PAGE with 2-mercaptoethanol. IEF-urea 8M was also evaluated. Using CSI it was shown that the marine alga released hemagglutinating compounds into the solutions; the same hemagglutinating compounds were also obtained by GLN. Ammonium sulfate precipitated proteins exhibited agglutinating activity against native and papain-treated human red blood cells. Temperature and EDTA were shown to affect dramatically the lectin activity towards red blood cells. A lectin was purified efficiently and the molecular weight calculated as approximately 78,000 Daltons. The CSI technique demonstrated that the alga could be returned to an active metabolic state by immersion in a simple buffer after having been kept dormant by freezing at −20°C for long periods. It was also shown that the alga was releasing bioactive compounds into the solutions and, therefore, this procedure is being suggested as a good, gentle, non-disruptive extraction technique and we postulate CSI as a possible bioreactor for the continuous production of bioactive compounds from green marine algae.

scholarly journals Effects of Culture Conditions on the Antibacterial Activity of Streptomyces Spp. against Erwinia Spp. Causing Soft Rot Disease on Asparagus Officinalis

2021 ◽  
Vol 4 (2) ◽  
pp. 1077-1086
Author(s):  
Nguyen Thanh Trung ◽  
Nguyen Thi Van Anh ◽  
Tran Thi Dao ◽  
Nguyen Thanh Huyen ◽  
Pham Le Anh Minh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ornamental Plants ◽  
Soft Rot ◽  
Antibacterial Activities ◽  
Culture Conditions ◽  
Asparagus Officinalis ◽  
Effective Control ◽  
Soft Rot Disease ◽  
Streptomyces Spp ◽  
Rot Disease

Erwinia is a genus of Enterobacteriacea containing mostly pathogens, which cause soft rot disease in many ornamental plants and crops, including Asparagus officinalis. Chemical treatments to control Erwinia have lost their attractiveness because of the development of resistant strains and the negative impacts on the environment and human health. Therefore, the study of biological controls of soft rot disease has gained great importance. There are several types of microorganisms that show activity against Erwinia spp. such as Pseudomonas fluorescence, Bacillus subtilis, and Streptomyces spp. Among them, Streptomyces spp. are found to be the most effective control agents. In this study, 64 isolates of Streptomyces were screened for their antibacterial activity against Erwinia spp. The results indicated that 18 isolates showed an antagonistic reaction against Erwinia spp. Among them, isolate D5.1 showed the highest inhibition activity. In addition, the morphological and antibacterial activities of isolate D5.1 grown in different conditions were also characterized. 

scholarly journals An Investigation of Moroccan Vinegars: Their Physicochemical Properties and Antioxidant and Antibacterial Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Driss Ousaaid ◽  
Hamada Imtara ◽  
Hassan Laaroussi ◽  
Badiaa Lyoussi ◽  
Ilham Elarabi
Keyword(s):  
Physicochemical Properties ◽  
Bioactive Compounds ◽  
Antibacterial Activities ◽  
Storage Conditions ◽  
Production Methods ◽  
Maximum Value ◽  
Processing And Storage ◽  
And Storage ◽  
Excellent Source

Vinegar is a natural product rich in bioactive compounds such as phenols, flavonoids, and organic acids. Several factors affect the quality of vinegars such as apple origin, environmental conditions, production methods, processing, and storage conditions. We investigated the quality of apple vinegars as well as their physicochemical properties and the antioxidant and antibacterial activities of vinegars collected from different areas in Morocco. For physicochemical properties, the outcomes showed the following values: pH (3.18–3.83), electrical conductivity (2.11–2.90), acidity (0.24–5.6), Brix (3.25–6), and density (1.0123–1.0233). The polyphenols content of samples was 68.91 mg AG/100 mL in sample S6 as the minimum value and 147.54 mg AG/100 mL in sample S2 as the maximum value. The best ability to scavenge the DPPH radical was observed in sample S3 (IC50 = 0.45 ± 0.013 µL/mL). Sample S2 showed moderate antibacterial effect against microorganisms tested with MICs ranging from 0.78 µL/mL to 1.125 µL/mL and with a diameter of inhibition ranging from 15.65 mm to 27.65 mm. In addition, a strong correlation was observed between the antibacterial activity of vinegars and physicochemical parameters (pH and total acidity). These outcomes have shown that our vinegar samples are an excellent source of bioactive compounds with potent antioxidant and antibacterial potentials.

Antibacterial Activities of Antibacterial Proteins/Peptides Isolated from Organs and Mucus of Clarias Gariepinus Reared at High Stocking Density

2012 ◽  
Vol 455-456 ◽  
pp. 455-460 ◽  
Author(s):  
Xiao Mei Wang ◽  
Wei Dai ◽  
Ke Zhing Xing ◽  
Tian Jun Li ◽  
Xin Wang
Keyword(s):  
Molecular Weight ◽  
Antibacterial Activity ◽  
Crude Protein ◽  
Clarias Gariepinus ◽  
Gel Filtration ◽  
Stocking Density ◽  
Antibacterial Activities ◽  
Edwardsiella Tarda ◽  
Bacterial Strains ◽  
Sds Page

. Antibacterial proteins/peptides are important parts of the innate immune system in Clarias gariepinus. To examine potential antibacterial proteins/peptides in organs and mucus of C. gariepinus, crude protein/peptide extracts were isolated with ammonium sulfate precipitation from mucus, skin, gill, suprabranchial organ and intestine. Following further extraction using Sephadex G-50 gel filtration chromatography, the proteins/peptides associated with two absorption peaks (AP1 and AP2) were pooled, respectively, and assayed for their antibacterial activities against Escherichia coli, Aeromonas hydrophila and Edwardsiella tarda. The results showed that AP1 and AP2 from all the sampled tissues and mucus at concentration of 100 mg mL-1 exhibited antibacterial activity against the tested bacterial strains. Differences in antibacterial activity were observed among sample extracts. The protein profiles of AP1 obtained by Tricine-SDS-PAGE gel showed a broad range of peptides/proteins, and molecular weight of the mutual abundant peptide obtained was about 27 kDa. Antibacterial activity of AP2 extracted from intestine was due to peptide with molecular weight of 5.5 kDa.

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